Testicular function in cultured postnatal mouse testis fragments is similar to that of animals during the first wave of spermatogenesis

Background Previously, we evaluated optimal organ culture conditions to produce elongated spermatids in an in vitro mouse testis culture system. However, differences in testicular function between the cultured testis fragments and animal testis have not been determined. Methods To examine how closel...

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Veröffentlicht in:	Birth defects research 2019-03, Vol.111 (5), p.270-280
Hauptverfasser:	Pence, Lisa M., Schmitt, Thomas C., Beger, Richard D., Del Valle, Pedro L., Nakamura, Noriko
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Approximation Biomarkers Cell culture Cell Differentiation Differentiation (biology) Flow cytometry Fragments Gene expression Histology in vitro organ culture Liquid chromatography Male Mass spectrometry Mass spectroscopy Mice Mice, Inbred C57BL Organ culture Organ Culture Techniques - methods Primary Cell Culture - methods Seminiferous Tubules - metabolism Spermatids Spermatids - cytology Spermatogenesis Spermatogenesis - physiology Spermatozoa - cytology Testes Testis - metabolism Testis - physiology Testosterone testosterone levels Tubules
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