Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization
Aim To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP‐1). Methodology THP‐1 cells were treated with resin monomers at noncytotoxic concentrations for 48...
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| Veröffentlicht in: | International endodontic journal 2019-07, Vol.52 (7), p.987-998 |
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| creator | Kim, I.‐S. Park, H. C. Quan, H. Kim, Y. Wu, L. Yang, H.‐C. |
| description | Aim
To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP‐1).
Methodology
THP‐1 cells were treated with resin monomers at noncytotoxic concentrations for 48 h and were analysed for CD86 and CD206 expressions using flow cytometry. The cells were stimulated for polarization in the presence of resin monomers (co‐treatment) or after treatment with monomers (pre‐treatment). CD86 and CD206 mRNA in co‐treated cells was evaluated using quantitative real‐time polymerase chain reaction. The release of TNF‐α and TGF‐β by pre‐treated and co‐treated cells was assessed using enzyme‐linked immunosorbent assay. Morphological changes of macrophages during polarization were observed using bright‐field microscopy. One‐way analysis of variance was used for statistical analysis.
Results
TEGDMA (1 mmol L−1) and HEMA (2 mmol L−1) did not induce CD86 and CD206 expressions in THP‐1 cells but rather inhibited their expressions in the co‐treated cells. The inhibitory effects also appeared at the transcription level. However, the expression of surface markers was not affected by pre‐treatment with resin monomers. The release of TNF‐α and TGF‐β by M1‐ and M2‐stimulated cells, respectively, was suppressed by co‐treatment (P |
| doi_str_mv | 10.1111/iej.13088 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2179499414</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2237766802</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3538-61379e8cb4a8480bada52bbc6eb16ff92fbd2b86989695221742396a002bd81b3</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMouq4e_AMS8KKHaj7aNDmKrF8IXvQcknbqdkmbNemi9dcbXRUUnEuYmScvyYPQASWnNNVZC4tTyomUG2hCuSgyVii6iSaE5jxjUhY7aDfGBSGkIJxuox1OSsJZLicIZk0D1RCxb_AQWhjmo4Me8JMbK-9w3XZpZKowOjMANn2N52Md_Ov4SeJfW9_jLjV-OTdPgJfemdC-maH1_R7aaoyLsP91TtHj5ezh4jq7u7-6uTi_yypecJkJyksFsrK5kbkk1tSmYNZWAiwVTaNYY2tmpVBSCVUwRsuccSUMIczWklo-Rcfr3GXwzyuIg-7aWIFzpge_ijrdULlSefIyRUd_0IVfhT69TjPGy1IISViiTtZU-laMARq9DG1nwqgp0R_udXKvP90n9vArcWU7qH_Ib9kJOFsDL62D8f8kfTO7XUe-A-L6jro</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2237766802</pqid></control><display><type>article</type><title>Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization</title><source>Wiley Online Library Journals Frontfile Complete</source><creator>Kim, I.‐S. ; Park, H. C. ; Quan, H. ; Kim, Y. ; Wu, L. ; Yang, H.‐C.</creator><creatorcontrib>Kim, I.‐S. ; Park, H. C. ; Quan, H. ; Kim, Y. ; Wu, L. ; Yang, H.‐C.</creatorcontrib><description>Aim
To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP‐1).
Methodology
THP‐1 cells were treated with resin monomers at noncytotoxic concentrations for 48 h and were analysed for CD86 and CD206 expressions using flow cytometry. The cells were stimulated for polarization in the presence of resin monomers (co‐treatment) or after treatment with monomers (pre‐treatment). CD86 and CD206 mRNA in co‐treated cells was evaluated using quantitative real‐time polymerase chain reaction. The release of TNF‐α and TGF‐β by pre‐treated and co‐treated cells was assessed using enzyme‐linked immunosorbent assay. Morphological changes of macrophages during polarization were observed using bright‐field microscopy. One‐way analysis of variance was used for statistical analysis.
Results
TEGDMA (1 mmol L−1) and HEMA (2 mmol L−1) did not induce CD86 and CD206 expressions in THP‐1 cells but rather inhibited their expressions in the co‐treated cells. The inhibitory effects also appeared at the transcription level. However, the expression of surface markers was not affected by pre‐treatment with resin monomers. The release of TNF‐α and TGF‐β by M1‐ and M2‐stimulated cells, respectively, was suppressed by co‐treatment (P < 0.05). Microscopic studies revealed that co‐treatment with resin monomers suppressed polarization‐associated morphological changes such as cell volume increase.
Conclusions
TEGDMA and HEMA inhibited macrophage polarization to both M1 and M2 at the transcription level, and the inhibitory effects disappeared upon the removal of resin monomers from the cell culture.</description><identifier>ISSN: 0143-2885</identifier><identifier>EISSN: 1365-2591</identifier><identifier>DOI: 10.1111/iej.13088</identifier><identifier>PMID: 30703248</identifier><language>eng</language><publisher>England: Wiley Subscription Services, Inc</publisher><subject>CD86 antigen ; Cell culture ; Cell size ; Dental restorative materials ; Dentistry ; Endodontics ; Flow cytometry ; hydroxyethyl methacrylate ; macrophage ; Macrophages ; Monocytes ; Monomers ; Morphology ; Polarization ; Polymerase chain reaction ; Statistical analysis ; Surface markers ; Transcription ; Triethylene glycol dimethacrylate ; Tumor necrosis factor</subject><ispartof>International endodontic journal, 2019-07, Vol.52 (7), p.987-998</ispartof><rights>2019 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><rights>2019 International Endodontic Journal. Published by John Wiley & Sons Ltd.</rights><rights>Copyright © 2019 International Endodontic Journal. Published by John Wiley & Sons Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3538-61379e8cb4a8480bada52bbc6eb16ff92fbd2b86989695221742396a002bd81b3</citedby><cites>FETCH-LOGICAL-c3538-61379e8cb4a8480bada52bbc6eb16ff92fbd2b86989695221742396a002bd81b3</cites><orcidid>0000-0001-9420-7894</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fiej.13088$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fiej.13088$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30703248$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, I.‐S.</creatorcontrib><creatorcontrib>Park, H. C.</creatorcontrib><creatorcontrib>Quan, H.</creatorcontrib><creatorcontrib>Kim, Y.</creatorcontrib><creatorcontrib>Wu, L.</creatorcontrib><creatorcontrib>Yang, H.‐C.</creatorcontrib><title>Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization</title><title>International endodontic journal</title><addtitle>Int Endod J</addtitle><description>Aim
To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP‐1).
Methodology
THP‐1 cells were treated with resin monomers at noncytotoxic concentrations for 48 h and were analysed for CD86 and CD206 expressions using flow cytometry. The cells were stimulated for polarization in the presence of resin monomers (co‐treatment) or after treatment with monomers (pre‐treatment). CD86 and CD206 mRNA in co‐treated cells was evaluated using quantitative real‐time polymerase chain reaction. The release of TNF‐α and TGF‐β by pre‐treated and co‐treated cells was assessed using enzyme‐linked immunosorbent assay. Morphological changes of macrophages during polarization were observed using bright‐field microscopy. One‐way analysis of variance was used for statistical analysis.
Results
TEGDMA (1 mmol L−1) and HEMA (2 mmol L−1) did not induce CD86 and CD206 expressions in THP‐1 cells but rather inhibited their expressions in the co‐treated cells. The inhibitory effects also appeared at the transcription level. However, the expression of surface markers was not affected by pre‐treatment with resin monomers. The release of TNF‐α and TGF‐β by M1‐ and M2‐stimulated cells, respectively, was suppressed by co‐treatment (P < 0.05). Microscopic studies revealed that co‐treatment with resin monomers suppressed polarization‐associated morphological changes such as cell volume increase.
Conclusions
TEGDMA and HEMA inhibited macrophage polarization to both M1 and M2 at the transcription level, and the inhibitory effects disappeared upon the removal of resin monomers from the cell culture.</description><subject>CD86 antigen</subject><subject>Cell culture</subject><subject>Cell size</subject><subject>Dental restorative materials</subject><subject>Dentistry</subject><subject>Endodontics</subject><subject>Flow cytometry</subject><subject>hydroxyethyl methacrylate</subject><subject>macrophage</subject><subject>Macrophages</subject><subject>Monocytes</subject><subject>Monomers</subject><subject>Morphology</subject><subject>Polarization</subject><subject>Polymerase chain reaction</subject><subject>Statistical analysis</subject><subject>Surface markers</subject><subject>Transcription</subject><subject>Triethylene glycol dimethacrylate</subject><subject>Tumor necrosis factor</subject><issn>0143-2885</issn><issn>1365-2591</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAQhoMouq4e_AMS8KKHaj7aNDmKrF8IXvQcknbqdkmbNemi9dcbXRUUnEuYmScvyYPQASWnNNVZC4tTyomUG2hCuSgyVii6iSaE5jxjUhY7aDfGBSGkIJxuox1OSsJZLicIZk0D1RCxb_AQWhjmo4Me8JMbK-9w3XZpZKowOjMANn2N52Md_Ov4SeJfW9_jLjV-OTdPgJfemdC-maH1_R7aaoyLsP91TtHj5ezh4jq7u7-6uTi_yypecJkJyksFsrK5kbkk1tSmYNZWAiwVTaNYY2tmpVBSCVUwRsuccSUMIczWklo-Rcfr3GXwzyuIg-7aWIFzpge_ijrdULlSefIyRUd_0IVfhT69TjPGy1IISViiTtZU-laMARq9DG1nwqgp0R_udXKvP90n9vArcWU7qH_Ib9kJOFsDL62D8f8kfTO7XUe-A-L6jro</recordid><startdate>201907</startdate><enddate>201907</enddate><creator>Kim, I.‐S.</creator><creator>Park, H. C.</creator><creator>Quan, H.</creator><creator>Kim, Y.</creator><creator>Wu, L.</creator><creator>Yang, H.‐C.</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-9420-7894</orcidid></search><sort><creationdate>201907</creationdate><title>Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization</title><author>Kim, I.‐S. ; Park, H. C. ; Quan, H. ; Kim, Y. ; Wu, L. ; Yang, H.‐C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3538-61379e8cb4a8480bada52bbc6eb16ff92fbd2b86989695221742396a002bd81b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>CD86 antigen</topic><topic>Cell culture</topic><topic>Cell size</topic><topic>Dental restorative materials</topic><topic>Dentistry</topic><topic>Endodontics</topic><topic>Flow cytometry</topic><topic>hydroxyethyl methacrylate</topic><topic>macrophage</topic><topic>Macrophages</topic><topic>Monocytes</topic><topic>Monomers</topic><topic>Morphology</topic><topic>Polarization</topic><topic>Polymerase chain reaction</topic><topic>Statistical analysis</topic><topic>Surface markers</topic><topic>Transcription</topic><topic>Triethylene glycol dimethacrylate</topic><topic>Tumor necrosis factor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, I.‐S.</creatorcontrib><creatorcontrib>Park, H. C.</creatorcontrib><creatorcontrib>Quan, H.</creatorcontrib><creatorcontrib>Kim, Y.</creatorcontrib><creatorcontrib>Wu, L.</creatorcontrib><creatorcontrib>Yang, H.‐C.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>International endodontic journal</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, I.‐S.</au><au>Park, H. C.</au><au>Quan, H.</au><au>Kim, Y.</au><au>Wu, L.</au><au>Yang, H.‐C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization</atitle><jtitle>International endodontic journal</jtitle><addtitle>Int Endod J</addtitle><date>2019-07</date><risdate>2019</risdate><volume>52</volume><issue>7</issue><spage>987</spage><epage>998</epage><pages>987-998</pages><issn>0143-2885</issn><eissn>1365-2591</eissn><abstract>Aim
To evaluate the effects of hydrophilic dental resin monomers, triethylene glycol dimethacrylate (TEGDMA) and hydroxyethyl methacrylate (HEMA), on the polarization of a human monocyte cell line (THP‐1).
Methodology
THP‐1 cells were treated with resin monomers at noncytotoxic concentrations for 48 h and were analysed for CD86 and CD206 expressions using flow cytometry. The cells were stimulated for polarization in the presence of resin monomers (co‐treatment) or after treatment with monomers (pre‐treatment). CD86 and CD206 mRNA in co‐treated cells was evaluated using quantitative real‐time polymerase chain reaction. The release of TNF‐α and TGF‐β by pre‐treated and co‐treated cells was assessed using enzyme‐linked immunosorbent assay. Morphological changes of macrophages during polarization were observed using bright‐field microscopy. One‐way analysis of variance was used for statistical analysis.
Results
TEGDMA (1 mmol L−1) and HEMA (2 mmol L−1) did not induce CD86 and CD206 expressions in THP‐1 cells but rather inhibited their expressions in the co‐treated cells. The inhibitory effects also appeared at the transcription level. However, the expression of surface markers was not affected by pre‐treatment with resin monomers. The release of TNF‐α and TGF‐β by M1‐ and M2‐stimulated cells, respectively, was suppressed by co‐treatment (P < 0.05). Microscopic studies revealed that co‐treatment with resin monomers suppressed polarization‐associated morphological changes such as cell volume increase.
Conclusions
TEGDMA and HEMA inhibited macrophage polarization to both M1 and M2 at the transcription level, and the inhibitory effects disappeared upon the removal of resin monomers from the cell culture.</abstract><cop>England</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30703248</pmid><doi>10.1111/iej.13088</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0001-9420-7894</orcidid></addata></record> |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | CD86 antigen Cell culture Cell size Dental restorative materials Dentistry Endodontics Flow cytometry hydroxyethyl methacrylate macrophage Macrophages Monocytes Monomers Morphology Polarization Polymerase chain reaction Statistical analysis Surface markers Transcription Triethylene glycol dimethacrylate Tumor necrosis factor |
| title | Effects of triethylene glycol dimethacrylate and hydroxyethyl methacrylate on macrophage polarization |
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