Breast Cancer‐Derived Exosomes Reflect the Cell‐of‐Origin Phenotype
A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. These vesicles contain lipids, nucleic acids, and proteins, and are said to reflect the cell‐of‐origin. However, for the exosomal protein content, there is limited evidence in the literature to ver...
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| Veröffentlicht in: | Proteomics (Weinheim) 2019-04, Vol.19 (8), p.e1800180-n/a |
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| creator | Wen, Shu Wen Lima, Luize G. Lobb, Richard J. Norris, Emma L. Hastie, Marcus L. Krumeich, Sophie Möller, Andreas |
| description | A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. These vesicles contain lipids, nucleic acids, and proteins, and are said to reflect the cell‐of‐origin. However, for the exosomal protein content, there is limited evidence in the literature to verify this statement. Here, proteomic assessment combined with pathway‐enrichment analysis is used to demonstrate that the protein cargo of exosomes reflects the epithelial/mesenchymal phenotype of secreting breast cancer cells. Given that epithelial‐mesenchymal plasticity is known to implicate various stages of cancer progression, the results suggest that breast cancer subtypes with distinct epithelial and mesenchymal phenotypes may be distinguished by directly assessing the protein content of exosomes. Additionally, the work is a substantial step toward verifying the statement that cell‐derived exosomes reflect the phenotype of the cells‐of‐origin. |
| doi_str_mv | 10.1002/pmic.201800180 |
| format | Article |
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These vesicles contain lipids, nucleic acids, and proteins, and are said to reflect the cell‐of‐origin. However, for the exosomal protein content, there is limited evidence in the literature to verify this statement. Here, proteomic assessment combined with pathway‐enrichment analysis is used to demonstrate that the protein cargo of exosomes reflects the epithelial/mesenchymal phenotype of secreting breast cancer cells. Given that epithelial‐mesenchymal plasticity is known to implicate various stages of cancer progression, the results suggest that breast cancer subtypes with distinct epithelial and mesenchymal phenotypes may be distinguished by directly assessing the protein content of exosomes. 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Additionally, the work is a substantial step toward verifying the statement that cell‐derived exosomes reflect the phenotype of the cells‐of‐origin.</description><subject>Animals</subject><subject>Blotting, Western</subject><subject>breast cancer</subject><subject>Breast Neoplasms - metabolism</subject><subject>Breast Neoplasms - pathology</subject><subject>Breast Neoplasms - ultrastructure</subject><subject>Cell Line, Tumor</subject><subject>Chromatography, Liquid</subject><subject>Epithelial-Mesenchymal Transition - physiology</subject><subject>epithelial‐to‐mesenchymal transition</subject><subject>exosomes</subject><subject>Exosomes - metabolism</subject><subject>Exosomes - pathology</subject><subject>Exosomes - ultrastructure</subject><subject>Female</subject><subject>Humans</subject><subject>Mass Spectrometry</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microscopy, Electron, Transmission</subject><subject>proteomic</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkLtOwzAUhi0EolBYGVFGlhRfEjseIRSoVNQKwWw57gkNyg07BbrxCDwjT4Kjlq4M5zJ859fRh9AZwSOCMb1sq8KMKCYJ7msPHRFO4lAmnOzv9pgN0LFzrx4RiRSHaMAwF5QQcYQm1xa064JU1wbsz9f3DdjiHRbB-LNxTQUueIS8BNMF3RKCFMrSM03u28wWL0UdzJdQN926hRN0kOvSwel2DtHz7fgpvQ-ns7tJejUNTRQJHoqMC6xFQpOYZpHO8jg3MWgpJfdTRhJLQzUjXHCuI82SBdGUU8A60xpnhA3RxSa3tc3bClynqsIZ_5iuoVk5RYnoU1hCPTraoMY2zlnIVWuLStu1Ilj1-lSvT-30-YPzbfYqq2Cxw_98eSDeAB9FCet_4tT8YZIShmPOfgGfVn2U</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Wen, Shu Wen</creator><creator>Lima, Luize G.</creator><creator>Lobb, Richard J.</creator><creator>Norris, Emma L.</creator><creator>Hastie, Marcus L.</creator><creator>Krumeich, Sophie</creator><creator>Möller, Andreas</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8618-6998</orcidid></search><sort><creationdate>201904</creationdate><title>Breast Cancer‐Derived Exosomes Reflect the Cell‐of‐Origin Phenotype</title><author>Wen, Shu Wen ; Lima, Luize G. ; Lobb, Richard J. ; Norris, Emma L. ; Hastie, Marcus L. ; Krumeich, Sophie ; Möller, Andreas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4476-7b670a782852b4abf5fc5ea9996c5e94909c2a316766a4a38d1a262e0abaa0b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Blotting, Western</topic><topic>breast cancer</topic><topic>Breast Neoplasms - metabolism</topic><topic>Breast Neoplasms - pathology</topic><topic>Breast Neoplasms - ultrastructure</topic><topic>Cell Line, Tumor</topic><topic>Chromatography, Liquid</topic><topic>Epithelial-Mesenchymal Transition - physiology</topic><topic>epithelial‐to‐mesenchymal transition</topic><topic>exosomes</topic><topic>Exosomes - metabolism</topic><topic>Exosomes - pathology</topic><topic>Exosomes - ultrastructure</topic><topic>Female</topic><topic>Humans</topic><topic>Mass Spectrometry</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microscopy, Electron, Transmission</topic><topic>proteomic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wen, Shu Wen</creatorcontrib><creatorcontrib>Lima, Luize G.</creatorcontrib><creatorcontrib>Lobb, Richard J.</creatorcontrib><creatorcontrib>Norris, Emma L.</creatorcontrib><creatorcontrib>Hastie, Marcus L.</creatorcontrib><creatorcontrib>Krumeich, Sophie</creatorcontrib><creatorcontrib>Möller, Andreas</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wen, Shu Wen</au><au>Lima, Luize G.</au><au>Lobb, Richard J.</au><au>Norris, Emma L.</au><au>Hastie, Marcus L.</au><au>Krumeich, Sophie</au><au>Möller, Andreas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Breast Cancer‐Derived Exosomes Reflect the Cell‐of‐Origin Phenotype</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2019-04</date><risdate>2019</risdate><volume>19</volume><issue>8</issue><spage>e1800180</spage><epage>n/a</epage><pages>e1800180-n/a</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>A manner in which cells can communicate with each other is via secreted nanoparticles termed exosomes. 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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Blotting, Western breast cancer Breast Neoplasms - metabolism Breast Neoplasms - pathology Breast Neoplasms - ultrastructure Cell Line, Tumor Chromatography, Liquid Epithelial-Mesenchymal Transition - physiology epithelial‐to‐mesenchymal transition exosomes Exosomes - metabolism Exosomes - pathology Exosomes - ultrastructure Female Humans Mass Spectrometry Mice Mice, Inbred C57BL Microscopy, Electron, Transmission proteomic |
| title | Breast Cancer‐Derived Exosomes Reflect the Cell‐of‐Origin Phenotype |
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