Helicobacter pylori‐downregulated tumor necrosis factor receptor‐associated protein 1 mediates apoptosis of human gastric epithelial cells
Heat shock proteins (HSPs) are crucial proteins in maintaining the homeostasis of human gastric epithelial cells. Tumor necrosis factor receptor‐associated protein 1 (TRAP1), a member of the HSP90 family, has been shown to be involved in various crucial physiological processes, particularly against...
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| Veröffentlicht in: | Journal of cellular physiology 2019-09, Vol.234 (9), p.15698-15707 |
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| container_title | Journal of cellular physiology |
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| creator | Teng, Yongsheng Liu, Xin Han, Bin Ma, Qiang Liu, Yugang Kong, Hui Lv, Yipin Mao, Fangyuan Cheng, Ping Hao, Chuanjie Yang, Shiming Zhang, Jinyu Peng, Liusheng Zou, Quanming Zhuang, Yuan |
| description | Heat shock proteins (HSPs) are crucial proteins in maintaining the homeostasis of human gastric epithelial cells. Tumor necrosis factor receptor‐associated protein 1 (TRAP1), a member of the HSP90 family, has been shown to be involved in various crucial physiological processes, particularly against apoptosis. However, the regulation and function of TRAP1 in Helicobacter pylori infection is still unknown. Here, we found that TRAP1 expression was downregulated on human gastric epithelial cells during
H. pylori infection by real‐time polymerase chain reaction (PCR) and western blot analysis. Through virulence factors mutant
H. pylori strains infection and inhibitors screening, we found that
H. pylori vacuolating cytotoxin A (
vacA), but not cytotoxin‐associated gene A (
cagA) protein, induced human gastric epithelial cells to downregulate TRAP1 via P38MAPK pathway by real‐time PCR and western blot analysis. Furthermore, downregulation of TRAP1 with lentivirus carrying TRAP1 short hairpin RNA constructs impairs mitochondrial function, and increases apoptosis of gastric epithelial cells. The results indicate that
H. pylori vacA downregulated TRAP1 is involved in the regulation of gastric epithelial cell apoptosis.
Helicobacter pylori vacA‐induced gastric epithelial cells to downregulate tumor necrosis factor receptor‐associated protein 1 (TRAP1) via P38MAPK pathway. Downregulated TRAP1 expression impairs mitochondrial function and increases of activated (cleaved) caspase‐3 expression, which results in an increased apoptosis of gastric epithelial cells. |
| doi_str_mv | 10.1002/jcp.28223 |
| format | Article |
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H. pylori infection by real‐time polymerase chain reaction (PCR) and western blot analysis. Through virulence factors mutant
H. pylori strains infection and inhibitors screening, we found that
H. pylori vacuolating cytotoxin A (
vacA), but not cytotoxin‐associated gene A (
cagA) protein, induced human gastric epithelial cells to downregulate TRAP1 via P38MAPK pathway by real‐time PCR and western blot analysis. Furthermore, downregulation of TRAP1 with lentivirus carrying TRAP1 short hairpin RNA constructs impairs mitochondrial function, and increases apoptosis of gastric epithelial cells. The results indicate that
H. pylori vacA downregulated TRAP1 is involved in the regulation of gastric epithelial cell apoptosis.
Helicobacter pylori vacA‐induced gastric epithelial cells to downregulate tumor necrosis factor receptor‐associated protein 1 (TRAP1) via P38MAPK pathway. Downregulated TRAP1 expression impairs mitochondrial function and increases of activated (cleaved) caspase‐3 expression, which results in an increased apoptosis of gastric epithelial cells.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.28223</identifier><identifier>PMID: 30710368</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>Apoptosis ; Epithelial cells ; gastric epithelial cells ; Heat shock proteins ; Helicobacter pylori ; Homeostasis ; Hsp90 protein ; Infections ; Mitochondria ; Necrosis ; Polymerase chain reaction ; Proteins ; Ribonucleic acid ; RNA ; TRAP1 ; Tumor necrosis factor ; Tumor necrosis factor-TNF ; Tumors ; vacA ; Virulence ; Virulence factors</subject><ispartof>Journal of cellular physiology, 2019-09, Vol.234 (9), p.15698-15707</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4193-b7a168c880026ec4b20c0fff663e32537d1ac7b9aabc7729cc1e30d95b6de89d3</citedby><cites>FETCH-LOGICAL-c4193-b7a168c880026ec4b20c0fff663e32537d1ac7b9aabc7729cc1e30d95b6de89d3</cites><orcidid>0000-0001-7825-0032</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.28223$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.28223$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30710368$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Teng, Yongsheng</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Han, Bin</creatorcontrib><creatorcontrib>Ma, Qiang</creatorcontrib><creatorcontrib>Liu, Yugang</creatorcontrib><creatorcontrib>Kong, Hui</creatorcontrib><creatorcontrib>Lv, Yipin</creatorcontrib><creatorcontrib>Mao, Fangyuan</creatorcontrib><creatorcontrib>Cheng, Ping</creatorcontrib><creatorcontrib>Hao, Chuanjie</creatorcontrib><creatorcontrib>Yang, Shiming</creatorcontrib><creatorcontrib>Zhang, Jinyu</creatorcontrib><creatorcontrib>Peng, Liusheng</creatorcontrib><creatorcontrib>Zou, Quanming</creatorcontrib><creatorcontrib>Zhuang, Yuan</creatorcontrib><title>Helicobacter pylori‐downregulated tumor necrosis factor receptor‐associated protein 1 mediates apoptosis of human gastric epithelial cells</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Heat shock proteins (HSPs) are crucial proteins in maintaining the homeostasis of human gastric epithelial cells. Tumor necrosis factor receptor‐associated protein 1 (TRAP1), a member of the HSP90 family, has been shown to be involved in various crucial physiological processes, particularly against apoptosis. However, the regulation and function of TRAP1 in Helicobacter pylori infection is still unknown. Here, we found that TRAP1 expression was downregulated on human gastric epithelial cells during
H. pylori infection by real‐time polymerase chain reaction (PCR) and western blot analysis. Through virulence factors mutant
H. pylori strains infection and inhibitors screening, we found that
H. pylori vacuolating cytotoxin A (
vacA), but not cytotoxin‐associated gene A (
cagA) protein, induced human gastric epithelial cells to downregulate TRAP1 via P38MAPK pathway by real‐time PCR and western blot analysis. Furthermore, downregulation of TRAP1 with lentivirus carrying TRAP1 short hairpin RNA constructs impairs mitochondrial function, and increases apoptosis of gastric epithelial cells. The results indicate that
H. pylori vacA downregulated TRAP1 is involved in the regulation of gastric epithelial cell apoptosis.
Helicobacter pylori vacA‐induced gastric epithelial cells to downregulate tumor necrosis factor receptor‐associated protein 1 (TRAP1) via P38MAPK pathway. Downregulated TRAP1 expression impairs mitochondrial function and increases of activated (cleaved) caspase‐3 expression, which results in an increased apoptosis of gastric epithelial cells.</description><subject>Apoptosis</subject><subject>Epithelial cells</subject><subject>gastric epithelial cells</subject><subject>Heat shock proteins</subject><subject>Helicobacter pylori</subject><subject>Homeostasis</subject><subject>Hsp90 protein</subject><subject>Infections</subject><subject>Mitochondria</subject><subject>Necrosis</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>TRAP1</subject><subject>Tumor necrosis factor</subject><subject>Tumor necrosis factor-TNF</subject><subject>Tumors</subject><subject>vacA</subject><subject>Virulence</subject><subject>Virulence factors</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp1kb1u2zAUhYmiRe06GfoCAYEs6SCbP5IojoHRxAkCtEMyExR1ldCQRIWUYHjrEwR5xjxJqdjtUKATf-7Hw3PvQegrJUtKCFttTb9kBWP8A5pTIkWS5hn7iOaxRhOZpXSGvoSwJYRIyflnNONEUMLzYo5eNtBY40ptBvC43zfO27dfr5XbdR4ex0YPUOFhbJ3HHRjvgg24jnA8ezDQx03EdQjO2He2924A22GKW6imq4B17yI3vXQ1fhpb3eFHHQZvDYbeDk_RgW6wgaYJJ-hTrZsAp8d1gR6uvt-vN8ndj-ub9eVdYlIqeVIKTfPCFEXsMAeTlowYUtd1nnPgLOOiotqIUmpdGiGYNIYCJ5XMyryCQlZ8gS4OutHu8whhUK0NkwPdgRuDYlTItOAyfrZA5_-gWzf6LrpTjDFZZJQLGqlvB2qaUfBQq97bVvu9okRNIakYknoPKbJnR8WxjEP6S_5JJQKrA7CzDez_r6Ru1z8Pkr8BHbSg1g</recordid><startdate>201909</startdate><enddate>201909</enddate><creator>Teng, Yongsheng</creator><creator>Liu, Xin</creator><creator>Han, Bin</creator><creator>Ma, Qiang</creator><creator>Liu, Yugang</creator><creator>Kong, Hui</creator><creator>Lv, Yipin</creator><creator>Mao, Fangyuan</creator><creator>Cheng, Ping</creator><creator>Hao, Chuanjie</creator><creator>Yang, Shiming</creator><creator>Zhang, Jinyu</creator><creator>Peng, Liusheng</creator><creator>Zou, Quanming</creator><creator>Zhuang, Yuan</creator><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-7825-0032</orcidid></search><sort><creationdate>201909</creationdate><title>Helicobacter pylori‐downregulated tumor necrosis factor receptor‐associated protein 1 mediates apoptosis of human gastric epithelial cells</title><author>Teng, Yongsheng ; Liu, Xin ; Han, Bin ; Ma, Qiang ; Liu, Yugang ; Kong, Hui ; Lv, Yipin ; Mao, Fangyuan ; Cheng, Ping ; Hao, Chuanjie ; Yang, Shiming ; Zhang, Jinyu ; Peng, Liusheng ; Zou, Quanming ; Zhuang, Yuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4193-b7a168c880026ec4b20c0fff663e32537d1ac7b9aabc7729cc1e30d95b6de89d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Apoptosis</topic><topic>Epithelial cells</topic><topic>gastric epithelial cells</topic><topic>Heat shock proteins</topic><topic>Helicobacter pylori</topic><topic>Homeostasis</topic><topic>Hsp90 protein</topic><topic>Infections</topic><topic>Mitochondria</topic><topic>Necrosis</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>TRAP1</topic><topic>Tumor necrosis factor</topic><topic>Tumor necrosis factor-TNF</topic><topic>Tumors</topic><topic>vacA</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Teng, Yongsheng</creatorcontrib><creatorcontrib>Liu, Xin</creatorcontrib><creatorcontrib>Han, Bin</creatorcontrib><creatorcontrib>Ma, Qiang</creatorcontrib><creatorcontrib>Liu, Yugang</creatorcontrib><creatorcontrib>Kong, Hui</creatorcontrib><creatorcontrib>Lv, Yipin</creatorcontrib><creatorcontrib>Mao, Fangyuan</creatorcontrib><creatorcontrib>Cheng, Ping</creatorcontrib><creatorcontrib>Hao, Chuanjie</creatorcontrib><creatorcontrib>Yang, Shiming</creatorcontrib><creatorcontrib>Zhang, Jinyu</creatorcontrib><creatorcontrib>Peng, Liusheng</creatorcontrib><creatorcontrib>Zou, Quanming</creatorcontrib><creatorcontrib>Zhuang, Yuan</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Teng, Yongsheng</au><au>Liu, Xin</au><au>Han, Bin</au><au>Ma, Qiang</au><au>Liu, Yugang</au><au>Kong, Hui</au><au>Lv, Yipin</au><au>Mao, Fangyuan</au><au>Cheng, Ping</au><au>Hao, Chuanjie</au><au>Yang, Shiming</au><au>Zhang, Jinyu</au><au>Peng, Liusheng</au><au>Zou, Quanming</au><au>Zhuang, Yuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helicobacter pylori‐downregulated tumor necrosis factor receptor‐associated protein 1 mediates apoptosis of human gastric epithelial cells</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2019-09</date><risdate>2019</risdate><volume>234</volume><issue>9</issue><spage>15698</spage><epage>15707</epage><pages>15698-15707</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Heat shock proteins (HSPs) are crucial proteins in maintaining the homeostasis of human gastric epithelial cells. Tumor necrosis factor receptor‐associated protein 1 (TRAP1), a member of the HSP90 family, has been shown to be involved in various crucial physiological processes, particularly against apoptosis. However, the regulation and function of TRAP1 in Helicobacter pylori infection is still unknown. Here, we found that TRAP1 expression was downregulated on human gastric epithelial cells during
H. pylori infection by real‐time polymerase chain reaction (PCR) and western blot analysis. Through virulence factors mutant
H. pylori strains infection and inhibitors screening, we found that
H. pylori vacuolating cytotoxin A (
vacA), but not cytotoxin‐associated gene A (
cagA) protein, induced human gastric epithelial cells to downregulate TRAP1 via P38MAPK pathway by real‐time PCR and western blot analysis. Furthermore, downregulation of TRAP1 with lentivirus carrying TRAP1 short hairpin RNA constructs impairs mitochondrial function, and increases apoptosis of gastric epithelial cells. The results indicate that
H. pylori vacA downregulated TRAP1 is involved in the regulation of gastric epithelial cell apoptosis.
Helicobacter pylori vacA‐induced gastric epithelial cells to downregulate tumor necrosis factor receptor‐associated protein 1 (TRAP1) via P38MAPK pathway. Downregulated TRAP1 expression impairs mitochondrial function and increases of activated (cleaved) caspase‐3 expression, which results in an increased apoptosis of gastric epithelial cells.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30710368</pmid><doi>10.1002/jcp.28223</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-7825-0032</orcidid></addata></record> |
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| subjects | Apoptosis Epithelial cells gastric epithelial cells Heat shock proteins Helicobacter pylori Homeostasis Hsp90 protein Infections Mitochondria Necrosis Polymerase chain reaction Proteins Ribonucleic acid RNA TRAP1 Tumor necrosis factor Tumor necrosis factor-TNF Tumors vacA Virulence Virulence factors |
| title | Helicobacter pylori‐downregulated tumor necrosis factor receptor‐associated protein 1 mediates apoptosis of human gastric epithelial cells |
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