Effects of iron oxide (Fe3O4) nanoparticles on Escherichia coli antibiotic‐resistant strains
Aims Antibiotic resistance of different bacteria requires the development of alternative approaches for overcoming this phenomenon. The antibacterial effects of iron oxide (Fe3O4) nanoparticles (NPs) (from 50 to 250 μg ml−1) on Escherichia coli antibiotic‐resistant strains have been aimed. Methods a...
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Veröffentlicht in: | Journal of applied microbiology 2019-04, Vol.126 (4), p.1108-1116 |
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creator | Gabrielyan, L. Hakobyan, L. Hovhannisyan, A. Trchounian, A. |
description | Aims
Antibiotic resistance of different bacteria requires the development of alternative approaches for overcoming this phenomenon. The antibacterial effects of iron oxide (Fe3O4) nanoparticles (NPs) (from 50 to 250 μg ml−1) on Escherichia coli antibiotic‐resistant strains have been aimed.
Methods and Results
The study was performed with ampicillin‐resistant E. coli DH5α‐pUC18 and kanamycin‐resistant E. coli pARG‐25 stains. Specific growth rate of bacteria (μ), lag phase duration and colony‐forming units (CFU) were determined to evaluate growth properties. Fe3O4 NPs (average size of 10·64 ± 4·73 nm) coated with oleic acid and synthesized by modified co‐precipitation method were used. The medium pH, H+ efflux, membrane H+ conductance, redox potential determinations and H2 yield assay were done using potentiometer methods. Growth properties were changed by NPs in concentration‐dependent manner. NPs decreased (up to twofold) H+‐fluxes through bacterial membrane more in E. coli in the presence of the N,N′‐dicyclohexylcarbodiimide, inhibitor of ATPase, indicating that antibacterial activity of these NPs was connected with ATP‐associated metabolism. Membrane‐associated H2 production was lowered up to twofold. Moreover, the synergetic interactions of NPs and antibiotics were found: combination of NPs and antibiotics provided the higher H+ conductance, lower H+‐fluxes and H2 yield.
Conclusions
Fe3O4 NPs can be suggested as alternative antibacterial agents, which can substitute antibiotics in different applications.
Significance and Impact of the Study
The antibacterial effects of Fe3O4 NPs on the growth properties and membrane activity of E. coli antibiotic‐resistant strains have been demonstrated. These NPs have potential as antibacterial agents, which can substitute for antibiotics in bacterial disease treatment in biomedicine, pharmaceutical and environmental applications. |
doi_str_mv | 10.1111/jam.14214 |
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Antibiotic resistance of different bacteria requires the development of alternative approaches for overcoming this phenomenon. The antibacterial effects of iron oxide (Fe3O4) nanoparticles (NPs) (from 50 to 250 μg ml−1) on Escherichia coli antibiotic‐resistant strains have been aimed.
Methods and Results
The study was performed with ampicillin‐resistant E. coli DH5α‐pUC18 and kanamycin‐resistant E. coli pARG‐25 stains. Specific growth rate of bacteria (μ), lag phase duration and colony‐forming units (CFU) were determined to evaluate growth properties. Fe3O4 NPs (average size of 10·64 ± 4·73 nm) coated with oleic acid and synthesized by modified co‐precipitation method were used. The medium pH, H+ efflux, membrane H+ conductance, redox potential determinations and H2 yield assay were done using potentiometer methods. Growth properties were changed by NPs in concentration‐dependent manner. NPs decreased (up to twofold) H+‐fluxes through bacterial membrane more in E. coli in the presence of the N,N′‐dicyclohexylcarbodiimide, inhibitor of ATPase, indicating that antibacterial activity of these NPs was connected with ATP‐associated metabolism. Membrane‐associated H2 production was lowered up to twofold. Moreover, the synergetic interactions of NPs and antibiotics were found: combination of NPs and antibiotics provided the higher H+ conductance, lower H+‐fluxes and H2 yield.
Conclusions
Fe3O4 NPs can be suggested as alternative antibacterial agents, which can substitute antibiotics in different applications.
Significance and Impact of the Study
The antibacterial effects of Fe3O4 NPs on the growth properties and membrane activity of E. coli antibiotic‐resistant strains have been demonstrated. These NPs have potential as antibacterial agents, which can substitute for antibiotics in bacterial disease treatment in biomedicine, pharmaceutical and environmental applications.</description><identifier>ISSN: 1364-5072</identifier><identifier>EISSN: 1365-2672</identifier><identifier>DOI: 10.1111/jam.14214</identifier><identifier>PMID: 30703295</identifier><language>eng</language><publisher>England: Oxford University Press</publisher><subject>Adenosine triphosphatase ; Ampicillin ; Antibacterial activity ; Antibacterial agents ; Antibiotic resistance ; Antibiotics ; Bacteria ; Bacterial diseases ; Dicyclohexylcarbodiimide ; E coli ; Efflux ; Electric potential ; Escherichia coli ; Escherichia coli antibiotic‐resistant strains ; Fluxes ; Growth rate ; H+‐fluxes ; H2 production ; Hydrogen ; Hydrogen production ; iron oxide nanoparticles ; Iron oxides ; Kanamycin ; Lag phase ; mechanisms of action ; Medical treatment ; Membrane conductance ; Metabolism ; Nanoparticles ; Oleic acid ; physiology and bacterial growth ; Potentiometers ; Properties (attributes) ; Redox potential ; Resistance ; Strains (organisms) ; Substitutes</subject><ispartof>Journal of applied microbiology, 2019-04, Vol.126 (4), p.1108-1116</ispartof><rights>2019 The Society for Applied Microbiology</rights><rights>2019 The Society for Applied Microbiology.</rights><rights>Copyright © 2019 The Society for Applied Microbiology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3534-799743160cd2aa984f224fa7117dab58fdf2b13bc4a7cb7b328ae917bf2a49683</citedby><cites>FETCH-LOGICAL-c3534-799743160cd2aa984f224fa7117dab58fdf2b13bc4a7cb7b328ae917bf2a49683</cites><orcidid>0000-0002-0901-4893</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjam.14214$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjam.14214$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30703295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Gabrielyan, L.</creatorcontrib><creatorcontrib>Hakobyan, L.</creatorcontrib><creatorcontrib>Hovhannisyan, A.</creatorcontrib><creatorcontrib>Trchounian, A.</creatorcontrib><title>Effects of iron oxide (Fe3O4) nanoparticles on Escherichia coli antibiotic‐resistant strains</title><title>Journal of applied microbiology</title><addtitle>J Appl Microbiol</addtitle><description>Aims
Antibiotic resistance of different bacteria requires the development of alternative approaches for overcoming this phenomenon. The antibacterial effects of iron oxide (Fe3O4) nanoparticles (NPs) (from 50 to 250 μg ml−1) on Escherichia coli antibiotic‐resistant strains have been aimed.
Methods and Results
The study was performed with ampicillin‐resistant E. coli DH5α‐pUC18 and kanamycin‐resistant E. coli pARG‐25 stains. Specific growth rate of bacteria (μ), lag phase duration and colony‐forming units (CFU) were determined to evaluate growth properties. Fe3O4 NPs (average size of 10·64 ± 4·73 nm) coated with oleic acid and synthesized by modified co‐precipitation method were used. The medium pH, H+ efflux, membrane H+ conductance, redox potential determinations and H2 yield assay were done using potentiometer methods. Growth properties were changed by NPs in concentration‐dependent manner. NPs decreased (up to twofold) H+‐fluxes through bacterial membrane more in E. coli in the presence of the N,N′‐dicyclohexylcarbodiimide, inhibitor of ATPase, indicating that antibacterial activity of these NPs was connected with ATP‐associated metabolism. Membrane‐associated H2 production was lowered up to twofold. Moreover, the synergetic interactions of NPs and antibiotics were found: combination of NPs and antibiotics provided the higher H+ conductance, lower H+‐fluxes and H2 yield.
Conclusions
Fe3O4 NPs can be suggested as alternative antibacterial agents, which can substitute antibiotics in different applications.
Significance and Impact of the Study
The antibacterial effects of Fe3O4 NPs on the growth properties and membrane activity of E. coli antibiotic‐resistant strains have been demonstrated. These NPs have potential as antibacterial agents, which can substitute for antibiotics in bacterial disease treatment in biomedicine, pharmaceutical and environmental applications.</description><subject>Adenosine triphosphatase</subject><subject>Ampicillin</subject><subject>Antibacterial activity</subject><subject>Antibacterial agents</subject><subject>Antibiotic resistance</subject><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Bacterial diseases</subject><subject>Dicyclohexylcarbodiimide</subject><subject>E coli</subject><subject>Efflux</subject><subject>Electric potential</subject><subject>Escherichia coli</subject><subject>Escherichia coli antibiotic‐resistant strains</subject><subject>Fluxes</subject><subject>Growth rate</subject><subject>H+‐fluxes</subject><subject>H2 production</subject><subject>Hydrogen</subject><subject>Hydrogen production</subject><subject>iron oxide nanoparticles</subject><subject>Iron oxides</subject><subject>Kanamycin</subject><subject>Lag phase</subject><subject>mechanisms of action</subject><subject>Medical treatment</subject><subject>Membrane conductance</subject><subject>Metabolism</subject><subject>Nanoparticles</subject><subject>Oleic acid</subject><subject>physiology and bacterial growth</subject><subject>Potentiometers</subject><subject>Properties (attributes)</subject><subject>Redox potential</subject><subject>Resistance</subject><subject>Strains (organisms)</subject><subject>Substitutes</subject><issn>1364-5072</issn><issn>1365-2672</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10MtOAjEUBuDGaATRhS9gmriBxUBvM6VLQsBLMGx066TTaUPJMMV2iLLzEXxGn8QK6MLEbk5z8uXPyQ_AJUZ9HN9gKVd9zAhmR6CNaZYmJOPkePdnSYo4aYGzEJYIYYrS7BS0KOKIEpG2wfPEGK2aAJ2B1rsaujdbatidajpnPVjL2q2lb6yqdDQ1nAS10N6qhZVQucpCWTe2sC6Kz_cPr4MNTVzB0Hhp63AOToysgr44zA54mk4ex7fJbH5zNx7NEkVTyhIuBGcUZ0iVREoxZIYQZiTHmJeySIemNKTAtFBMclXwgpKh1ALzwhDJRDakHdDd5669e9no0OQrG5SuKllrtwk5wVywVCAmIr3-Q5du4-t4XVQCEy4op1H19kp5F4LXJl97u5J-m2OUf5eex9LzXenRXh0SN8VKl7_yp-UIBnvwaiu9_T8pvx897CO_AKXHizY</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Gabrielyan, L.</creator><creator>Hakobyan, L.</creator><creator>Hovhannisyan, A.</creator><creator>Trchounian, A.</creator><general>Oxford University Press</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7TM</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-0901-4893</orcidid></search><sort><creationdate>201904</creationdate><title>Effects of iron oxide (Fe3O4) nanoparticles on Escherichia coli antibiotic‐resistant strains</title><author>Gabrielyan, L. ; Hakobyan, L. ; Hovhannisyan, A. ; Trchounian, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3534-799743160cd2aa984f224fa7117dab58fdf2b13bc4a7cb7b328ae917bf2a49683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adenosine triphosphatase</topic><topic>Ampicillin</topic><topic>Antibacterial activity</topic><topic>Antibacterial agents</topic><topic>Antibiotic resistance</topic><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Bacterial diseases</topic><topic>Dicyclohexylcarbodiimide</topic><topic>E coli</topic><topic>Efflux</topic><topic>Electric potential</topic><topic>Escherichia coli</topic><topic>Escherichia coli antibiotic‐resistant strains</topic><topic>Fluxes</topic><topic>Growth rate</topic><topic>H+‐fluxes</topic><topic>H2 production</topic><topic>Hydrogen</topic><topic>Hydrogen production</topic><topic>iron oxide nanoparticles</topic><topic>Iron oxides</topic><topic>Kanamycin</topic><topic>Lag phase</topic><topic>mechanisms of action</topic><topic>Medical treatment</topic><topic>Membrane conductance</topic><topic>Metabolism</topic><topic>Nanoparticles</topic><topic>Oleic acid</topic><topic>physiology and bacterial growth</topic><topic>Potentiometers</topic><topic>Properties (attributes)</topic><topic>Redox potential</topic><topic>Resistance</topic><topic>Strains (organisms)</topic><topic>Substitutes</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gabrielyan, L.</creatorcontrib><creatorcontrib>Hakobyan, L.</creatorcontrib><creatorcontrib>Hovhannisyan, A.</creatorcontrib><creatorcontrib>Trchounian, A.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of applied microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gabrielyan, L.</au><au>Hakobyan, L.</au><au>Hovhannisyan, A.</au><au>Trchounian, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of iron oxide (Fe3O4) nanoparticles on Escherichia coli antibiotic‐resistant strains</atitle><jtitle>Journal of applied microbiology</jtitle><addtitle>J Appl Microbiol</addtitle><date>2019-04</date><risdate>2019</risdate><volume>126</volume><issue>4</issue><spage>1108</spage><epage>1116</epage><pages>1108-1116</pages><issn>1364-5072</issn><eissn>1365-2672</eissn><abstract>Aims
Antibiotic resistance of different bacteria requires the development of alternative approaches for overcoming this phenomenon. The antibacterial effects of iron oxide (Fe3O4) nanoparticles (NPs) (from 50 to 250 μg ml−1) on Escherichia coli antibiotic‐resistant strains have been aimed.
Methods and Results
The study was performed with ampicillin‐resistant E. coli DH5α‐pUC18 and kanamycin‐resistant E. coli pARG‐25 stains. Specific growth rate of bacteria (μ), lag phase duration and colony‐forming units (CFU) were determined to evaluate growth properties. Fe3O4 NPs (average size of 10·64 ± 4·73 nm) coated with oleic acid and synthesized by modified co‐precipitation method were used. The medium pH, H+ efflux, membrane H+ conductance, redox potential determinations and H2 yield assay were done using potentiometer methods. Growth properties were changed by NPs in concentration‐dependent manner. NPs decreased (up to twofold) H+‐fluxes through bacterial membrane more in E. coli in the presence of the N,N′‐dicyclohexylcarbodiimide, inhibitor of ATPase, indicating that antibacterial activity of these NPs was connected with ATP‐associated metabolism. Membrane‐associated H2 production was lowered up to twofold. Moreover, the synergetic interactions of NPs and antibiotics were found: combination of NPs and antibiotics provided the higher H+ conductance, lower H+‐fluxes and H2 yield.
Conclusions
Fe3O4 NPs can be suggested as alternative antibacterial agents, which can substitute antibiotics in different applications.
Significance and Impact of the Study
The antibacterial effects of Fe3O4 NPs on the growth properties and membrane activity of E. coli antibiotic‐resistant strains have been demonstrated. These NPs have potential as antibacterial agents, which can substitute for antibiotics in bacterial disease treatment in biomedicine, pharmaceutical and environmental applications.</abstract><cop>England</cop><pub>Oxford University Press</pub><pmid>30703295</pmid><doi>10.1111/jam.14214</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-0901-4893</orcidid></addata></record> |
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subjects | Adenosine triphosphatase Ampicillin Antibacterial activity Antibacterial agents Antibiotic resistance Antibiotics Bacteria Bacterial diseases Dicyclohexylcarbodiimide E coli Efflux Electric potential Escherichia coli Escherichia coli antibiotic‐resistant strains Fluxes Growth rate H+‐fluxes H2 production Hydrogen Hydrogen production iron oxide nanoparticles Iron oxides Kanamycin Lag phase mechanisms of action Medical treatment Membrane conductance Metabolism Nanoparticles Oleic acid physiology and bacterial growth Potentiometers Properties (attributes) Redox potential Resistance Strains (organisms) Substitutes |
title | Effects of iron oxide (Fe3O4) nanoparticles on Escherichia coli antibiotic‐resistant strains |
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