Effect of epigallocatechin-3- gallate solutions on bond durability at the adhesive interface in caries-affected dentin
Hydrolytic and enzymatic degradation by matrix metalloproteinases (MMPs) reduces the durability of composite resin restorations on caries-affected dentin (CAD). The use of MMP inhibitors such as epigallocatechin-3-gallate (EGCG) could increase the longevity of the bond to dentin. This study aimed to...
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| Veröffentlicht in: | Journal of the mechanical behavior of biomedical materials 2019-03, Vol.91, p.398-405 |
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| creator | Fialho, Melissa Proença Nogueira Hass, Viviane Nogueira, Rodrigo Proença França, Fabiana Mantovani Gomes Turssi, Cecilia Pedroso Basting, Roberta Tarkany Amaral, Flávia Lucisano Botelho |
| description | Hydrolytic and enzymatic degradation by matrix metalloproteinases (MMPs) reduces the durability of composite resin restorations on caries-affected dentin (CAD). The use of MMP inhibitors such as epigallocatechin-3-gallate (EGCG) could increase the longevity of the bond to dentin. This study aimed to evaluate the use of EGCG at different aqueous concentrations on the resin-dentin microtensile bond strength (μTBS), fracture pattern and nanoleakage (NL) in immediate (IM) time interval and after 12-months of water storage (1Y) when using a two-step etch-and-rinse adhesive system on CAD. Dentin surfaces of 40 human molars were submitted to a microbiological caries induction protocol and randomized into 5 groups (n = 8) (0.02% EGCG; 0.2% EGCG; 0.5% EGCG; 2% Chlorhexidine [CHX] and no treatment as Control Group - [NT]). After acid etching, the solutions were applied for 60 s followed by application of dental adhesive (Adper Single Bond 2, 3 M ESPE) to CAD surfaces. Subsequently, a resin composite (4 mm) block was built on the dentin. After 24 h, the teeth were sectioned into beam-shaped specimens (cross-sectional area of 1 mm
and 8-mm high). Half of the specimens were tested in IM and the other half after 1Y. Two samples per tooth were submitted to SEM for NL evaluation. Data were statistically analyzed by two-way ANOVA and Tukey tests (α = 0.05). The results showed that use of EGCG and CHX did not affect μTBS in IM (p > 0.05). After 1Y, there was a reduction in μTBS for all experimental groups (p |
| doi_str_mv | 10.1016/j.jmbbm.2018.11.022 |
| format | Article |
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and 8-mm high). Half of the specimens were tested in IM and the other half after 1Y. Two samples per tooth were submitted to SEM for NL evaluation. Data were statistically analyzed by two-way ANOVA and Tukey tests (α = 0.05). The results showed that use of EGCG and CHX did not affect μTBS in IM (p > 0.05). After 1Y, there was a reduction in μTBS for all experimental groups (p < 0.05). Adhesive fractures predominated in IM in all groups, except for 0.05% EGCG and NT. After 1Y, there was an increase in these adhesive fractures in all groups. For NL, all agents applied reduced NL in comparison with CT (p < 0.001). CHX showed lower NL (p < 0.001), followed by 0.02% and 0.5% EGCG. NT showed highest NL for both time intervals (p < 0.001). Thus, although the use of EGCG at different concentrations and CHX reduced the NL, they were unable to reduce degradation of μTBS to CAD over time.</description><identifier>ISSN: 1751-6161</identifier><identifier>EISSN: 1878-0180</identifier><identifier>DOI: 10.1016/j.jmbbm.2018.11.022</identifier><identifier>PMID: 30669058</identifier><language>eng</language><publisher>Netherlands</publisher><ispartof>Journal of the mechanical behavior of biomedical materials, 2019-03, Vol.91, p.398-405</ispartof><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c371t-eb026f91c448f3bdcce911889b3cb645e7ab8b7f1f5738ecb1e7472b5855e67d3</citedby><cites>FETCH-LOGICAL-c371t-eb026f91c448f3bdcce911889b3cb645e7ab8b7f1f5738ecb1e7472b5855e67d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30669058$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fialho, Melissa Proença Nogueira</creatorcontrib><creatorcontrib>Hass, Viviane</creatorcontrib><creatorcontrib>Nogueira, Rodrigo Proença</creatorcontrib><creatorcontrib>França, Fabiana Mantovani Gomes</creatorcontrib><creatorcontrib>Turssi, Cecilia Pedroso</creatorcontrib><creatorcontrib>Basting, Roberta Tarkany</creatorcontrib><creatorcontrib>Amaral, Flávia Lucisano Botelho</creatorcontrib><title>Effect of epigallocatechin-3- gallate solutions on bond durability at the adhesive interface in caries-affected dentin</title><title>Journal of the mechanical behavior of biomedical materials</title><addtitle>J Mech Behav Biomed Mater</addtitle><description>Hydrolytic and enzymatic degradation by matrix metalloproteinases (MMPs) reduces the durability of composite resin restorations on caries-affected dentin (CAD). The use of MMP inhibitors such as epigallocatechin-3-gallate (EGCG) could increase the longevity of the bond to dentin. This study aimed to evaluate the use of EGCG at different aqueous concentrations on the resin-dentin microtensile bond strength (μTBS), fracture pattern and nanoleakage (NL) in immediate (IM) time interval and after 12-months of water storage (1Y) when using a two-step etch-and-rinse adhesive system on CAD. Dentin surfaces of 40 human molars were submitted to a microbiological caries induction protocol and randomized into 5 groups (n = 8) (0.02% EGCG; 0.2% EGCG; 0.5% EGCG; 2% Chlorhexidine [CHX] and no treatment as Control Group - [NT]). After acid etching, the solutions were applied for 60 s followed by application of dental adhesive (Adper Single Bond 2, 3 M ESPE) to CAD surfaces. Subsequently, a resin composite (4 mm) block was built on the dentin. After 24 h, the teeth were sectioned into beam-shaped specimens (cross-sectional area of 1 mm
and 8-mm high). Half of the specimens were tested in IM and the other half after 1Y. Two samples per tooth were submitted to SEM for NL evaluation. Data were statistically analyzed by two-way ANOVA and Tukey tests (α = 0.05). The results showed that use of EGCG and CHX did not affect μTBS in IM (p > 0.05). After 1Y, there was a reduction in μTBS for all experimental groups (p < 0.05). Adhesive fractures predominated in IM in all groups, except for 0.05% EGCG and NT. After 1Y, there was an increase in these adhesive fractures in all groups. For NL, all agents applied reduced NL in comparison with CT (p < 0.001). CHX showed lower NL (p < 0.001), followed by 0.02% and 0.5% EGCG. NT showed highest NL for both time intervals (p < 0.001). Thus, although the use of EGCG at different concentrations and CHX reduced the NL, they were unable to reduce degradation of μTBS to CAD over time.</description><issn>1751-6161</issn><issn>1878-0180</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo9kEtLxTAQhYMovn-BIFm6ac00bZIuRXyB4EbXIUkn3lza5tqkF_z39vpazcxhzhzmI-QCWAkMxPW6XA_WDmXFQJUAJauqPXIMSqpiUdj-0ssGCgECjshJSmvGBGNKHZIjzoRoWaOOyfbOe3SZRk9xE95N30dnMrpVGAte0J2wjDTFfs4hjonGkdo4drSbJ2NDH_InNZnmFVLTrTCFLdIwZpy8cbuOOjMFTIX5jsHFh2MO4xk58KZPeP5bT8nb_d3r7WPx_PLwdHvzXDguIRdoWSV8C66ulee2cw5bAKVay50VdYPSWGWlB99IrtBZQFnLyjaqaVDIjp-Sq5-7myl-zJiyHkJyuDw1YpyTrkC2dVW1ollW-c-qm2JKE3q9mcJgpk8NTO-A67X-Bq53wDWAXoAvrsvfgNkO2P17_gjzLwKkf30</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Fialho, Melissa Proença Nogueira</creator><creator>Hass, Viviane</creator><creator>Nogueira, Rodrigo Proença</creator><creator>França, Fabiana Mantovani Gomes</creator><creator>Turssi, Cecilia Pedroso</creator><creator>Basting, Roberta Tarkany</creator><creator>Amaral, Flávia Lucisano Botelho</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201903</creationdate><title>Effect of epigallocatechin-3- gallate solutions on bond durability at the adhesive interface in caries-affected dentin</title><author>Fialho, Melissa Proença Nogueira ; Hass, Viviane ; Nogueira, Rodrigo Proença ; França, Fabiana Mantovani Gomes ; Turssi, Cecilia Pedroso ; Basting, Roberta Tarkany ; Amaral, Flávia Lucisano Botelho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c371t-eb026f91c448f3bdcce911889b3cb645e7ab8b7f1f5738ecb1e7472b5855e67d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fialho, Melissa Proença Nogueira</creatorcontrib><creatorcontrib>Hass, Viviane</creatorcontrib><creatorcontrib>Nogueira, Rodrigo Proença</creatorcontrib><creatorcontrib>França, Fabiana Mantovani Gomes</creatorcontrib><creatorcontrib>Turssi, Cecilia Pedroso</creatorcontrib><creatorcontrib>Basting, Roberta Tarkany</creatorcontrib><creatorcontrib>Amaral, Flávia Lucisano Botelho</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the mechanical behavior of biomedical materials</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fialho, Melissa Proença Nogueira</au><au>Hass, Viviane</au><au>Nogueira, Rodrigo Proença</au><au>França, Fabiana Mantovani Gomes</au><au>Turssi, Cecilia Pedroso</au><au>Basting, Roberta Tarkany</au><au>Amaral, Flávia Lucisano Botelho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of epigallocatechin-3- gallate solutions on bond durability at the adhesive interface in caries-affected dentin</atitle><jtitle>Journal of the mechanical behavior of biomedical materials</jtitle><addtitle>J Mech Behav Biomed Mater</addtitle><date>2019-03</date><risdate>2019</risdate><volume>91</volume><spage>398</spage><epage>405</epage><pages>398-405</pages><issn>1751-6161</issn><eissn>1878-0180</eissn><abstract>Hydrolytic and enzymatic degradation by matrix metalloproteinases (MMPs) reduces the durability of composite resin restorations on caries-affected dentin (CAD). The use of MMP inhibitors such as epigallocatechin-3-gallate (EGCG) could increase the longevity of the bond to dentin. This study aimed to evaluate the use of EGCG at different aqueous concentrations on the resin-dentin microtensile bond strength (μTBS), fracture pattern and nanoleakage (NL) in immediate (IM) time interval and after 12-months of water storage (1Y) when using a two-step etch-and-rinse adhesive system on CAD. Dentin surfaces of 40 human molars were submitted to a microbiological caries induction protocol and randomized into 5 groups (n = 8) (0.02% EGCG; 0.2% EGCG; 0.5% EGCG; 2% Chlorhexidine [CHX] and no treatment as Control Group - [NT]). After acid etching, the solutions were applied for 60 s followed by application of dental adhesive (Adper Single Bond 2, 3 M ESPE) to CAD surfaces. Subsequently, a resin composite (4 mm) block was built on the dentin. After 24 h, the teeth were sectioned into beam-shaped specimens (cross-sectional area of 1 mm
and 8-mm high). Half of the specimens were tested in IM and the other half after 1Y. Two samples per tooth were submitted to SEM for NL evaluation. Data were statistically analyzed by two-way ANOVA and Tukey tests (α = 0.05). The results showed that use of EGCG and CHX did not affect μTBS in IM (p > 0.05). After 1Y, there was a reduction in μTBS for all experimental groups (p < 0.05). Adhesive fractures predominated in IM in all groups, except for 0.05% EGCG and NT. After 1Y, there was an increase in these adhesive fractures in all groups. For NL, all agents applied reduced NL in comparison with CT (p < 0.001). CHX showed lower NL (p < 0.001), followed by 0.02% and 0.5% EGCG. NT showed highest NL for both time intervals (p < 0.001). Thus, although the use of EGCG at different concentrations and CHX reduced the NL, they were unable to reduce degradation of μTBS to CAD over time.</abstract><cop>Netherlands</cop><pmid>30669058</pmid><doi>10.1016/j.jmbbm.2018.11.022</doi><tpages>8</tpages></addata></record> |
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| title | Effect of epigallocatechin-3- gallate solutions on bond durability at the adhesive interface in caries-affected dentin |
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