Protein and gene expression of relevant enzymes and nuclear receptor of hepatic lipid metabolism in grazing dairy cattle during the transition period
We aimed to study the protein and gene expression of some hepatic enzymes of lipid metabolism along with plasma biomarkers in grazing dairy cattle during the transition period. Blood and liver biopsies from a group of eight multiparous cows were sampled at −28, −14, +4, +14, +28 and +56 days relativ...
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| Veröffentlicht in: | Research in veterinary science 2019-04, Vol.123, p.223-231 |
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| creator | Angeli, E. Trionfini, V. Gareis, N.C. Matiller, V. Huber, E. Rey, F. Salvetti, N.R. Ortega, H.H. Hein, G.J. |
| description | We aimed to study the protein and gene expression of some hepatic enzymes of lipid metabolism along with plasma biomarkers in grazing dairy cattle during the transition period. Blood and liver biopsies from a group of eight multiparous cows were sampled at −28, −14, +4, +14, +28 and +56 days relative to parturition. Peak concentrations of NEFA and beta-hydroxybutyric acid with high triacylglycerol content in the liver were recorded on day 4 postpartum. Consistent with blood biomarkers, the gene expression of carnitine palmitoyltransferase 1A (CPT1A) and acyl-CoA oxidase 1 (ACOX1) increased, whereas that of diacylglycerol O-acyltransferase 1 (DGAT1) decreased. Nevertheless, CPT1A protein expression did not change during all the period evaluated and ACOX1 protein expression increased on day 56 postpartum. In addition, the protein expression of peroxisome proliferator-activated receptor alpha (PPAR-alpha) increased on day 28 postpartum. On the other hand, DGAT1 protein expression decreased on day 14 postpartum. As expected, the expression of genes associated with fatty acid oxidation increased on the first days postpartum but, notably, protein expression was highest after transition. Since most infectious diseases and metabolic disorders in dairy cattle occur particularly on the first days postpartum, it is not so clear whether an increase in the oxidation capacity of the liver at that time could help to prevent disease and improve dairy production. The valuable results about protein expression of enzymes involved in liver lipid metabolism could help to better characterize the metabolism of dairy cattle during the transition period.
•Protein expression of liver lipid metabolism was evaluated in dairy cows.•The expression of genes involved in fatty acid oxidation was higher close to calving.•Protein expression of liver lipid metabolism was higher after the transition period. |
| doi_str_mv | 10.1016/j.rvsc.2019.01.020 |
| format | Article |
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•Protein expression of liver lipid metabolism was evaluated in dairy cows.•The expression of genes involved in fatty acid oxidation was higher close to calving.•Protein expression of liver lipid metabolism was higher after the transition period.</description><identifier>ISSN: 0034-5288</identifier><identifier>EISSN: 1532-2661</identifier><identifier>DOI: 10.1016/j.rvsc.2019.01.020</identifier><identifier>PMID: 30684909</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Acyl-CoA oxidase ; Animal lactation ; Bioindicators ; Biomarkers ; Biotechnology ; Blood ; Bovidae ; Carnitine ; Carnitine palmitoyltransferase ; Cattle ; Dairy cattle ; Diacylglycerol O-acyltransferase ; Diet ; Diglycerides ; Enzymes ; Fatty acids ; Gene expression ; Grazing ; Immunoglobulins ; Infectious diseases ; Laboratory animals ; Lipid metabolism ; Lipids ; Liver ; Medical research ; Metabolic disorders ; Metabolism ; Metabolites ; Milk ; Oxidation ; Palmitoyltransferase ; Parturition ; Peroxisome proliferator-activated receptors ; Postpartum ; PPAR-alpha ; Protein expression ; Proteins ; Researchers ; Soybeans ; Studies ; Transition period ; Triglycerides ; Veterinary medicine</subject><ispartof>Research in veterinary science, 2019-04, Vol.123, p.223-231</ispartof><rights>2019 Elsevier Ltd</rights><rights>Copyright © 2019. Published by Elsevier Ltd.</rights><rights>Copyright Elsevier Limited Apr 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-1e118807e2e9b5b9dabb0afb38cb9e60ae84f334f8e82d84967530978efc970f3</citedby><cites>FETCH-LOGICAL-c384t-1e118807e2e9b5b9dabb0afb38cb9e60ae84f334f8e82d84967530978efc970f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.rvsc.2019.01.020$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30684909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Angeli, E.</creatorcontrib><creatorcontrib>Trionfini, V.</creatorcontrib><creatorcontrib>Gareis, N.C.</creatorcontrib><creatorcontrib>Matiller, V.</creatorcontrib><creatorcontrib>Huber, E.</creatorcontrib><creatorcontrib>Rey, F.</creatorcontrib><creatorcontrib>Salvetti, N.R.</creatorcontrib><creatorcontrib>Ortega, H.H.</creatorcontrib><creatorcontrib>Hein, G.J.</creatorcontrib><title>Protein and gene expression of relevant enzymes and nuclear receptor of hepatic lipid metabolism in grazing dairy cattle during the transition period</title><title>Research in veterinary science</title><addtitle>Res Vet Sci</addtitle><description>We aimed to study the protein and gene expression of some hepatic enzymes of lipid metabolism along with plasma biomarkers in grazing dairy cattle during the transition period. Blood and liver biopsies from a group of eight multiparous cows were sampled at −28, −14, +4, +14, +28 and +56 days relative to parturition. Peak concentrations of NEFA and beta-hydroxybutyric acid with high triacylglycerol content in the liver were recorded on day 4 postpartum. Consistent with blood biomarkers, the gene expression of carnitine palmitoyltransferase 1A (CPT1A) and acyl-CoA oxidase 1 (ACOX1) increased, whereas that of diacylglycerol O-acyltransferase 1 (DGAT1) decreased. Nevertheless, CPT1A protein expression did not change during all the period evaluated and ACOX1 protein expression increased on day 56 postpartum. In addition, the protein expression of peroxisome proliferator-activated receptor alpha (PPAR-alpha) increased on day 28 postpartum. On the other hand, DGAT1 protein expression decreased on day 14 postpartum. As expected, the expression of genes associated with fatty acid oxidation increased on the first days postpartum but, notably, protein expression was highest after transition. Since most infectious diseases and metabolic disorders in dairy cattle occur particularly on the first days postpartum, it is not so clear whether an increase in the oxidation capacity of the liver at that time could help to prevent disease and improve dairy production. The valuable results about protein expression of enzymes involved in liver lipid metabolism could help to better characterize the metabolism of dairy cattle during the transition period.
•Protein expression of liver lipid metabolism was evaluated in dairy cows.•The expression of genes involved in fatty acid oxidation was higher close to calving.•Protein expression of liver lipid metabolism was higher after the transition period.</description><subject>Acyl-CoA oxidase</subject><subject>Animal lactation</subject><subject>Bioindicators</subject><subject>Biomarkers</subject><subject>Biotechnology</subject><subject>Blood</subject><subject>Bovidae</subject><subject>Carnitine</subject><subject>Carnitine palmitoyltransferase</subject><subject>Cattle</subject><subject>Dairy cattle</subject><subject>Diacylglycerol O-acyltransferase</subject><subject>Diet</subject><subject>Diglycerides</subject><subject>Enzymes</subject><subject>Fatty acids</subject><subject>Gene expression</subject><subject>Grazing</subject><subject>Immunoglobulins</subject><subject>Infectious diseases</subject><subject>Laboratory animals</subject><subject>Lipid metabolism</subject><subject>Lipids</subject><subject>Liver</subject><subject>Medical research</subject><subject>Metabolic disorders</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Milk</subject><subject>Oxidation</subject><subject>Palmitoyltransferase</subject><subject>Parturition</subject><subject>Peroxisome proliferator-activated receptors</subject><subject>Postpartum</subject><subject>PPAR-alpha</subject><subject>Protein expression</subject><subject>Proteins</subject><subject>Researchers</subject><subject>Soybeans</subject><subject>Studies</subject><subject>Transition period</subject><subject>Triglycerides</subject><subject>Veterinary medicine</subject><issn>0034-5288</issn><issn>1532-2661</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kcFu1DAQhiMEotvCC3BAlrhwyTK2s4ktcUEVFKRKcICz5diTrVeJHWxnxfY9eF8ctnDgwMmS_c3vmfmq6gWFLQXavjls4zGZLQMqt0C3wOBRtaE7zmrWtvRxtQHgTb1jQlxUlykdAKChtHtaXXBoRSNBbqqfX2LI6DzR3pI9eiT4Y46YkguehIFEHPGofSbo708Tpt-cX8yIOpZHg3MOcQXvcNbZGTK62VkyYdZ9GF2aSMneR33v_J5Y7eKJGJ3ziMQucb3Ld0hy1D65vH45Y3TBPqueDHpM-PzhvKq-fXj_9fpjffv55tP1u9vacNHkmiKlQkCHDGW_66XVfQ966LkwvcQWNIpm4LwZBApmy8Rtt-MgO4GDkR0M_Kp6fc6dY_i-YMpqcsngOGqPYUmK0U42ZdeMF_TVP-ghLNGX7golaQkVsFLsTJkYUoo4qDm6SceToqBWaeqgVmlqlaaAqiKtFL18iF76Ce3fkj-WCvD2DGDZxdFhVMk49AatKwqyssH9L_8X_LGrcA</recordid><startdate>20190401</startdate><enddate>20190401</enddate><creator>Angeli, E.</creator><creator>Trionfini, V.</creator><creator>Gareis, N.C.</creator><creator>Matiller, V.</creator><creator>Huber, E.</creator><creator>Rey, F.</creator><creator>Salvetti, N.R.</creator><creator>Ortega, H.H.</creator><creator>Hein, G.J.</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QP</scope><scope>7QR</scope><scope>7T5</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20190401</creationdate><title>Protein and gene expression of relevant enzymes and nuclear receptor of hepatic lipid metabolism in grazing dairy cattle during the transition period</title><author>Angeli, E. ; Trionfini, V. ; Gareis, N.C. ; Matiller, V. ; Huber, E. ; Rey, F. ; Salvetti, N.R. ; Ortega, H.H. ; Hein, G.J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-1e118807e2e9b5b9dabb0afb38cb9e60ae84f334f8e82d84967530978efc970f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Acyl-CoA oxidase</topic><topic>Animal lactation</topic><topic>Bioindicators</topic><topic>Biomarkers</topic><topic>Biotechnology</topic><topic>Blood</topic><topic>Bovidae</topic><topic>Carnitine</topic><topic>Carnitine palmitoyltransferase</topic><topic>Cattle</topic><topic>Dairy cattle</topic><topic>Diacylglycerol O-acyltransferase</topic><topic>Diet</topic><topic>Diglycerides</topic><topic>Enzymes</topic><topic>Fatty acids</topic><topic>Gene expression</topic><topic>Grazing</topic><topic>Immunoglobulins</topic><topic>Infectious diseases</topic><topic>Laboratory animals</topic><topic>Lipid metabolism</topic><topic>Lipids</topic><topic>Liver</topic><topic>Medical research</topic><topic>Metabolic disorders</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Milk</topic><topic>Oxidation</topic><topic>Palmitoyltransferase</topic><topic>Parturition</topic><topic>Peroxisome proliferator-activated receptors</topic><topic>Postpartum</topic><topic>PPAR-alpha</topic><topic>Protein expression</topic><topic>Proteins</topic><topic>Researchers</topic><topic>Soybeans</topic><topic>Studies</topic><topic>Transition period</topic><topic>Triglycerides</topic><topic>Veterinary medicine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angeli, E.</creatorcontrib><creatorcontrib>Trionfini, V.</creatorcontrib><creatorcontrib>Gareis, N.C.</creatorcontrib><creatorcontrib>Matiller, V.</creatorcontrib><creatorcontrib>Huber, E.</creatorcontrib><creatorcontrib>Rey, F.</creatorcontrib><creatorcontrib>Salvetti, N.R.</creatorcontrib><creatorcontrib>Ortega, H.H.</creatorcontrib><creatorcontrib>Hein, G.J.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Research in veterinary science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angeli, E.</au><au>Trionfini, V.</au><au>Gareis, N.C.</au><au>Matiller, V.</au><au>Huber, E.</au><au>Rey, F.</au><au>Salvetti, N.R.</au><au>Ortega, H.H.</au><au>Hein, G.J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Protein and gene expression of relevant enzymes and nuclear receptor of hepatic lipid metabolism in grazing dairy cattle during the transition period</atitle><jtitle>Research in veterinary science</jtitle><addtitle>Res Vet Sci</addtitle><date>2019-04-01</date><risdate>2019</risdate><volume>123</volume><spage>223</spage><epage>231</epage><pages>223-231</pages><issn>0034-5288</issn><eissn>1532-2661</eissn><abstract>We aimed to study the protein and gene expression of some hepatic enzymes of lipid metabolism along with plasma biomarkers in grazing dairy cattle during the transition period. Blood and liver biopsies from a group of eight multiparous cows were sampled at −28, −14, +4, +14, +28 and +56 days relative to parturition. Peak concentrations of NEFA and beta-hydroxybutyric acid with high triacylglycerol content in the liver were recorded on day 4 postpartum. Consistent with blood biomarkers, the gene expression of carnitine palmitoyltransferase 1A (CPT1A) and acyl-CoA oxidase 1 (ACOX1) increased, whereas that of diacylglycerol O-acyltransferase 1 (DGAT1) decreased. Nevertheless, CPT1A protein expression did not change during all the period evaluated and ACOX1 protein expression increased on day 56 postpartum. In addition, the protein expression of peroxisome proliferator-activated receptor alpha (PPAR-alpha) increased on day 28 postpartum. On the other hand, DGAT1 protein expression decreased on day 14 postpartum. As expected, the expression of genes associated with fatty acid oxidation increased on the first days postpartum but, notably, protein expression was highest after transition. Since most infectious diseases and metabolic disorders in dairy cattle occur particularly on the first days postpartum, it is not so clear whether an increase in the oxidation capacity of the liver at that time could help to prevent disease and improve dairy production. The valuable results about protein expression of enzymes involved in liver lipid metabolism could help to better characterize the metabolism of dairy cattle during the transition period.
•Protein expression of liver lipid metabolism was evaluated in dairy cows.•The expression of genes involved in fatty acid oxidation was higher close to calving.•Protein expression of liver lipid metabolism was higher after the transition period.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>30684909</pmid><doi>10.1016/j.rvsc.2019.01.020</doi><tpages>9</tpages></addata></record> |
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| subjects | Acyl-CoA oxidase Animal lactation Bioindicators Biomarkers Biotechnology Blood Bovidae Carnitine Carnitine palmitoyltransferase Cattle Dairy cattle Diacylglycerol O-acyltransferase Diet Diglycerides Enzymes Fatty acids Gene expression Grazing Immunoglobulins Infectious diseases Laboratory animals Lipid metabolism Lipids Liver Medical research Metabolic disorders Metabolism Metabolites Milk Oxidation Palmitoyltransferase Parturition Peroxisome proliferator-activated receptors Postpartum PPAR-alpha Protein expression Proteins Researchers Soybeans Studies Transition period Triglycerides Veterinary medicine |
| title | Protein and gene expression of relevant enzymes and nuclear receptor of hepatic lipid metabolism in grazing dairy cattle during the transition period |
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