Utilization of the ComRS system for the rapid markerless deletion of chromosomal genes in Streptococcus suis
To develop a markerless gene deletion strategy in Streptococcus suis to solve the problem that several serotypes against electrotransformation of foreign DNA. Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper...
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| Veröffentlicht in: | Future microbiology 2019-02, Vol.14 (3), p.207-222 |
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| container_title | Future microbiology |
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| creator | Zhu, Yinchu Dong, Wenyang Ma, Jiale Zhang, Yue Pan, Zihao Yao, Huochun |
| description | To develop a markerless gene deletion strategy in Streptococcus suis to solve the problem that several serotypes against electrotransformation of foreign DNA.
Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper and lower homologous fragments was amplification by fusion-PCR for spectinomycin-positive and sucrose-negative selection during gene deletion.
Three phylogenetic clusters of ComR were identified to function for natural transformation by specific recognition to competence pheromone in S. suis. Thus, they were employed to establish gene deletion method. Its efficiency for genetic replacement was dependent on the length of homologs fragment and the concentration of donor DNA. This rapid gene-editing technique may greatly facilitate molecular studies on S. suis. |
| doi_str_mv | 10.2217/fmb-2018-0279 |
| format | Article |
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Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper and lower homologous fragments was amplification by fusion-PCR for spectinomycin-positive and sucrose-negative selection during gene deletion.
Three phylogenetic clusters of ComR were identified to function for natural transformation by specific recognition to competence pheromone in S. suis. Thus, they were employed to establish gene deletion method. Its efficiency for genetic replacement was dependent on the length of homologs fragment and the concentration of donor DNA. This rapid gene-editing technique may greatly facilitate molecular studies on S. suis.</description><identifier>ISSN: 1746-0913</identifier><identifier>EISSN: 1746-0921</identifier><identifier>DOI: 10.2217/fmb-2018-0279</identifier><identifier>PMID: 30663887</identifier><language>eng</language><publisher>England: Future Medicine Ltd</publisher><subject>Antibiotics ; Bacteria ; Binding sites ; Bioinformatics ; Chromosome deletion ; Deoxyribonucleic acid ; DNA ; Gene deletion ; Genes ; Genetic engineering ; Genome editing ; Glycerol ; Hogs ; Meningitis ; Negative selection ; Peptides ; Pheromones ; Phylogeny ; Plasmids ; Sepsis ; Serotypes ; Signal transduction ; Spectinomycin ; Streptococcus infections ; Streptococcus suis ; Sucrose ; Sugar</subject><ispartof>Future microbiology, 2019-02, Vol.14 (3), p.207-222</ispartof><rights>Copyright Future Medicine Ltd Feb 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c321t-17191a24de707eb19d9abba6a359b39cfeb3dd7662115fc842e9e1b23c6bab6e3</citedby><cites>FETCH-LOGICAL-c321t-17191a24de707eb19d9abba6a359b39cfeb3dd7662115fc842e9e1b23c6bab6e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30663887$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhu, Yinchu</creatorcontrib><creatorcontrib>Dong, Wenyang</creatorcontrib><creatorcontrib>Ma, Jiale</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Pan, Zihao</creatorcontrib><creatorcontrib>Yao, Huochun</creatorcontrib><title>Utilization of the ComRS system for the rapid markerless deletion of chromosomal genes in Streptococcus suis</title><title>Future microbiology</title><addtitle>Future Microbiol</addtitle><description>To develop a markerless gene deletion strategy in Streptococcus suis to solve the problem that several serotypes against electrotransformation of foreign DNA.
Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper and lower homologous fragments was amplification by fusion-PCR for spectinomycin-positive and sucrose-negative selection during gene deletion.
Three phylogenetic clusters of ComR were identified to function for natural transformation by specific recognition to competence pheromone in S. suis. Thus, they were employed to establish gene deletion method. Its efficiency for genetic replacement was dependent on the length of homologs fragment and the concentration of donor DNA. This rapid gene-editing technique may greatly facilitate molecular studies on S. suis.</description><subject>Antibiotics</subject><subject>Bacteria</subject><subject>Binding sites</subject><subject>Bioinformatics</subject><subject>Chromosome deletion</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gene deletion</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genome editing</subject><subject>Glycerol</subject><subject>Hogs</subject><subject>Meningitis</subject><subject>Negative selection</subject><subject>Peptides</subject><subject>Pheromones</subject><subject>Phylogeny</subject><subject>Plasmids</subject><subject>Sepsis</subject><subject>Serotypes</subject><subject>Signal transduction</subject><subject>Spectinomycin</subject><subject>Streptococcus infections</subject><subject>Streptococcus suis</subject><subject>Sucrose</subject><subject>Sugar</subject><issn>1746-0913</issn><issn>1746-0921</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkTlPxDAQhS0EYmGhpEWWaGgCPhI7LtGKS0JC4qgj25lAIIkXT1Isvx4vxxZUMxp98_T0HiFHnJ0JwfV507tMMF5mTGizRfa4zlXGjODbm53LGdlHfGOsKLnhu2QmmVKyLPUe6Z7Htms_7diGgYaGjq9AF6F_eKS4whF62oT4fYx22da0t_EdYgeItIYO_r78awx9wNDbjr7AAEjbgT6OEZZj8MH7CSlOLR6QncZ2CIe_c06ery6fFjfZ3f317eLiLvNS8DHjOrm0Iq9BMw2Om9pY56yysjBOGt-Ak3WtlRKcF40vcwEGuBPSK2edAjknpz-6yxg-JsCx6lv00HV2gDBhlXIzOZOy0Ak9-Ye-hSkOyV2V4lWFyEUuE5X9UD4GxAhNtYxtymJVcbbmdJVqqNY1VOsaEn_8qzq5HuoN_Ze7_AI9EIRt</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Zhu, Yinchu</creator><creator>Dong, Wenyang</creator><creator>Ma, Jiale</creator><creator>Zhang, Yue</creator><creator>Pan, Zihao</creator><creator>Yao, Huochun</creator><general>Future Medicine Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>EHMNL</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB0</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>Utilization of the ComRS system for the rapid markerless deletion of chromosomal genes in Streptococcus suis</title><author>Zhu, Yinchu ; Dong, Wenyang ; Ma, Jiale ; Zhang, Yue ; Pan, Zihao ; Yao, Huochun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c321t-17191a24de707eb19d9abba6a359b39cfeb3dd7662115fc842e9e1b23c6bab6e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antibiotics</topic><topic>Bacteria</topic><topic>Binding sites</topic><topic>Bioinformatics</topic><topic>Chromosome deletion</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Gene deletion</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genome editing</topic><topic>Glycerol</topic><topic>Hogs</topic><topic>Meningitis</topic><topic>Negative selection</topic><topic>Peptides</topic><topic>Pheromones</topic><topic>Phylogeny</topic><topic>Plasmids</topic><topic>Sepsis</topic><topic>Serotypes</topic><topic>Signal transduction</topic><topic>Spectinomycin</topic><topic>Streptococcus infections</topic><topic>Streptococcus suis</topic><topic>Sucrose</topic><topic>Sugar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhu, Yinchu</creatorcontrib><creatorcontrib>Dong, Wenyang</creatorcontrib><creatorcontrib>Ma, Jiale</creatorcontrib><creatorcontrib>Zhang, Yue</creatorcontrib><creatorcontrib>Pan, Zihao</creatorcontrib><creatorcontrib>Yao, Huochun</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>ProQuest Public Health Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>UK & Ireland Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><jtitle>Future microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhu, Yinchu</au><au>Dong, Wenyang</au><au>Ma, Jiale</au><au>Zhang, Yue</au><au>Pan, Zihao</au><au>Yao, Huochun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization of the ComRS system for the rapid markerless deletion of chromosomal genes in Streptococcus suis</atitle><jtitle>Future microbiology</jtitle><addtitle>Future Microbiol</addtitle><date>2019-02</date><risdate>2019</risdate><volume>14</volume><issue>3</issue><spage>207</spage><epage>222</epage><pages>207-222</pages><issn>1746-0913</issn><eissn>1746-0921</eissn><abstract>To develop a markerless gene deletion strategy in Streptococcus suis to solve the problem that several serotypes against electrotransformation of foreign DNA.
Bioinformatics retrieval was performed to identified ComRS systems functioning for natural transformation. A sacB-spc cassette with the upper and lower homologous fragments was amplification by fusion-PCR for spectinomycin-positive and sucrose-negative selection during gene deletion.
Three phylogenetic clusters of ComR were identified to function for natural transformation by specific recognition to competence pheromone in S. suis. Thus, they were employed to establish gene deletion method. Its efficiency for genetic replacement was dependent on the length of homologs fragment and the concentration of donor DNA. This rapid gene-editing technique may greatly facilitate molecular studies on S. suis.</abstract><cop>England</cop><pub>Future Medicine Ltd</pub><pmid>30663887</pmid><doi>10.2217/fmb-2018-0279</doi><tpages>16</tpages></addata></record> |
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| subjects | Antibiotics Bacteria Binding sites Bioinformatics Chromosome deletion Deoxyribonucleic acid DNA Gene deletion Genes Genetic engineering Genome editing Glycerol Hogs Meningitis Negative selection Peptides Pheromones Phylogeny Plasmids Sepsis Serotypes Signal transduction Spectinomycin Streptococcus infections Streptococcus suis Sucrose Sugar |
| title | Utilization of the ComRS system for the rapid markerless deletion of chromosomal genes in Streptococcus suis |
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