Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels
Auriculataoside A, an anthracenone dimer glycoside isolated from Cassia auriculata seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC 50 value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action...
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| Veröffentlicht in: | Journal of natural medicines 2019-06, Vol.73 (3), p.450-455 |
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| creator | Wang, Weicheng Nakashima, Souichi Nakamura, Seikou Oda, Yoshimi Matsuda, Hisashi |
| description | Auriculataoside A, an anthracenone dimer glycoside isolated from
Cassia auriculata
seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC
50
value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells. |
| doi_str_mv | 10.1007/s11418-018-01278-0 |
| format | Article |
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Cassia auriculata
seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC
50
value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells.</description><identifier>ISSN: 1340-3443</identifier><identifier>EISSN: 1861-0293</identifier><identifier>DOI: 10.1007/s11418-018-01278-0</identifier><identifier>PMID: 30627935</identifier><language>eng</language><publisher>Singapore: Springer Singapore</publisher><subject>Animals ; Biomedical and Life Sciences ; Biomedicine ; cdc42 GTP-Binding Protein - metabolism ; Cell cycle ; Cell Line, Tumor ; Complementary & Alternative Medicine ; Herbal medicine ; Humans ; Medicinal Chemistry ; Melanoma ; Melanoma, Experimental - drug therapy ; Melanoma, Experimental - pathology ; Organic chemicals ; Original Paper ; Pharmacology/Toxicology ; Pharmacy ; Phytochemicals ; Plant Sciences ; Proteins ; rhoA GTP-Binding Protein - metabolism</subject><ispartof>Journal of natural medicines, 2019-06, Vol.73 (3), p.450-455</ispartof><rights>The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2019</rights><rights>The Japanese Society of Pharmacognosy and Springer Japan KK, part of Springer Nature 2019.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c492t-84a9c9faefc6b4756b65a4fd41569a610cc18d8e7486e939f7c0106159cfa1683</citedby><cites>FETCH-LOGICAL-c492t-84a9c9faefc6b4756b65a4fd41569a610cc18d8e7486e939f7c0106159cfa1683</cites><orcidid>0000-0003-4217-065X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11418-018-01278-0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11418-018-01278-0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30627935$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Weicheng</creatorcontrib><creatorcontrib>Nakashima, Souichi</creatorcontrib><creatorcontrib>Nakamura, Seikou</creatorcontrib><creatorcontrib>Oda, Yoshimi</creatorcontrib><creatorcontrib>Matsuda, Hisashi</creatorcontrib><title>Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels</title><title>Journal of natural medicines</title><addtitle>J Nat Med</addtitle><addtitle>J Nat Med</addtitle><description>Auriculataoside A, an anthracenone dimer glycoside isolated from
Cassia auriculata
seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC
50
value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells.</description><subject>Animals</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>cdc42 GTP-Binding Protein - metabolism</subject><subject>Cell cycle</subject><subject>Cell Line, Tumor</subject><subject>Complementary & Alternative Medicine</subject><subject>Herbal medicine</subject><subject>Humans</subject><subject>Medicinal Chemistry</subject><subject>Melanoma</subject><subject>Melanoma, Experimental - drug therapy</subject><subject>Melanoma, Experimental - pathology</subject><subject>Organic chemicals</subject><subject>Original Paper</subject><subject>Pharmacology/Toxicology</subject><subject>Pharmacy</subject><subject>Phytochemicals</subject><subject>Plant Sciences</subject><subject>Proteins</subject><subject>rhoA GTP-Binding Protein - metabolism</subject><issn>1340-3443</issn><issn>1861-0293</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kcFuFSEUhidGY2v1BVwYEjduxnKAAWY53tRq0qSJadcTLnO40jDDFWaadOfOB_ANfZIyvVUTFy4Oh4Tv_D_wV9VroO-BUnWaAQTomj4UU2V9Uh2DllBT1vKnZc8FrbkQ_Kh6kfMNpYJxDs-rI04lUy1vjqsf3TT7ep9i8A6Tmf0tEnQO7UyiI2ZJ3i7BzCZmPyDpSJzIB5BkxGCmOBoiuoZYDCGT7R3Jy36fMGdfqDK9Gaxgv77__GIsrO1r7Ej2u8kEP-1I8ZzRTyTgLYb8snrmTMj46rGfVNcfz642n-qLy_PPm-6itqJlc62FaW3rDDort0I1cisbI9wgoJGtkUCtBT1oVEJLbHnrlKVAJTStdQak5ifVu4Nusf-2YJ770ef1AWbCuOSewfovoGFF3_6D3sQlldsXinGqmVKgCsUOlE0x54Su3yc_mnTXA-3XmPpDTD19qBJTT8vQm0fpZTvi8Gfkdy4F4Acgl6Nph-mv939k7wGA_Z6S</recordid><startdate>20190601</startdate><enddate>20190601</enddate><creator>Wang, Weicheng</creator><creator>Nakashima, Souichi</creator><creator>Nakamura, Seikou</creator><creator>Oda, Yoshimi</creator><creator>Matsuda, Hisashi</creator><general>Springer Singapore</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-4217-065X</orcidid></search><sort><creationdate>20190601</creationdate><title>Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels</title><author>Wang, Weicheng ; Nakashima, Souichi ; Nakamura, Seikou ; Oda, Yoshimi ; Matsuda, Hisashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c492t-84a9c9faefc6b4756b65a4fd41569a610cc18d8e7486e939f7c0106159cfa1683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>cdc42 GTP-Binding Protein - metabolism</topic><topic>Cell cycle</topic><topic>Cell Line, Tumor</topic><topic>Complementary & Alternative Medicine</topic><topic>Herbal medicine</topic><topic>Humans</topic><topic>Medicinal Chemistry</topic><topic>Melanoma</topic><topic>Melanoma, Experimental - drug therapy</topic><topic>Melanoma, Experimental - pathology</topic><topic>Organic chemicals</topic><topic>Original Paper</topic><topic>Pharmacology/Toxicology</topic><topic>Pharmacy</topic><topic>Phytochemicals</topic><topic>Plant Sciences</topic><topic>Proteins</topic><topic>rhoA GTP-Binding Protein - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Weicheng</creatorcontrib><creatorcontrib>Nakashima, Souichi</creatorcontrib><creatorcontrib>Nakamura, Seikou</creatorcontrib><creatorcontrib>Oda, Yoshimi</creatorcontrib><creatorcontrib>Matsuda, Hisashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of natural medicines</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Weicheng</au><au>Nakashima, Souichi</au><au>Nakamura, Seikou</au><au>Oda, Yoshimi</au><au>Matsuda, Hisashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels</atitle><jtitle>Journal of natural medicines</jtitle><stitle>J Nat Med</stitle><addtitle>J Nat Med</addtitle><date>2019-06-01</date><risdate>2019</risdate><volume>73</volume><issue>3</issue><spage>450</spage><epage>455</epage><pages>450-455</pages><issn>1340-3443</issn><eissn>1861-0293</eissn><abstract>Auriculataoside A, an anthracenone dimer glycoside isolated from
Cassia auriculata
seed, shows anti-proliferative effects on cell line B16 melanoma 4A5 cells with an IC
50
value of 0.82 μM. However, it shows no such effect on normal human dermal fibroblast (HDF) cells. To evaluate the mode of action underlying the anti-proliferative effect of auriculataoside A on cells, we examined changes in whole protein expression after treatment with auriculataoside A and found that the expression Cdc42, RhoA, and Rac1, which are Rho family GTPases, was reduced. Auriculataoside A also arrested the cell cycle at G1 phase. These results suggest that the suppression of the above proteins induced G1 arrest. In addition, auriculataoside A also suppressed the expression of β-catenin and c-Myc proteins. This action of auriculataoside A could be one of the mechanisms underlying its selective anti-proliferative effect on B16 melanoma cells.</abstract><cop>Singapore</cop><pub>Springer Singapore</pub><pmid>30627935</pmid><doi>10.1007/s11418-018-01278-0</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0003-4217-065X</orcidid></addata></record> |
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| subjects | Animals Biomedical and Life Sciences Biomedicine cdc42 GTP-Binding Protein - metabolism Cell cycle Cell Line, Tumor Complementary & Alternative Medicine Herbal medicine Humans Medicinal Chemistry Melanoma Melanoma, Experimental - drug therapy Melanoma, Experimental - pathology Organic chemicals Original Paper Pharmacology/Toxicology Pharmacy Phytochemicals Plant Sciences Proteins rhoA GTP-Binding Protein - metabolism |
| title | Anti-proliferative effect of auriculataoside A on B16 melanoma 4A5 cells by suppression of Cdc42–Rac1–RhoA signaling protein levels |
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