Genetic Diversity of the Cryptococcus gattii Species Complex in Mato Grosso State, Brazil
Cryptococcosis is caused by fungi of the genus Cryptococcus . Owing to its importance, this study aimed to analyze the genetic diversity of C. gattii isolates from animals, humans, and the environment in Mato Grosso State (MT), Brazil, during November 2010–December 2017. All isolates of the C. gatti...
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| Veröffentlicht in: | Mycopathologia (1975) 2019-02, Vol.184 (1), p.45-51 |
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| creator | Maruyama, Fernanda Harumi de Paula, Daphine Ariadne Jesus Menezes, Isabela de Godoy Favalessa, Olívia Cometti Hahn, Rosane Christine de Almeida, Arleana do Bom Parto Ferreira Sousa, Valéria Régia Franco Nakazato, Luciano Dutra, Valéria |
| description | Cryptococcosis is caused by fungi of the genus
Cryptococcus
. Owing to its importance, this study aimed to analyze the genetic diversity of
C. gattii
isolates from animals, humans, and the environment in Mato Grosso State (MT), Brazil, during November 2010–December 2017. All isolates of the
C. gattii
species complex were subjected to molecular genotyping via Restriction Fragment Length Polymorphism (PCR–RFLP) and Multi-locus Sequence Typing (MLST). PCR–RFLP analysis revealed that 21 isolates presented the genotype VGII, which is considered the most common and virulent genotype globally among. MLST analysis revealed the presence of 14 sequence types (STs), of which 5 are considered new genotypes. Clonal Complex (CC) CC182 (
n
= 5; 23,80%) and CC309 (
n
= 3; 14,28%) were the most frequent. CC distribution in relation to origin revealed that three CCs were found in animals with a predominance of CC182 (66,66%), while nine were found in humans, and two CCs were found in the environment. Extensive genetic variability was observed among the isolates in the State of Mato Grosso. STs belonging to the already described clonal complexes (CC) indicate the global expansion and adaptation of isolates in several other countries. Therefore, detection of clonal complexes and STs already described in other regions and the occurrence of new STs in the present study help further the current understanding of the geographic dispersion and genetic origin of the
C. gattii
species complex. |
| doi_str_mv | 10.1007/s11046-018-0313-2 |
| format | Article |
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Cryptococcus
. Owing to its importance, this study aimed to analyze the genetic diversity of
C. gattii
isolates from animals, humans, and the environment in Mato Grosso State (MT), Brazil, during November 2010–December 2017. All isolates of the
C. gattii
species complex were subjected to molecular genotyping via Restriction Fragment Length Polymorphism (PCR–RFLP) and Multi-locus Sequence Typing (MLST). PCR–RFLP analysis revealed that 21 isolates presented the genotype VGII, which is considered the most common and virulent genotype globally among. MLST analysis revealed the presence of 14 sequence types (STs), of which 5 are considered new genotypes. Clonal Complex (CC) CC182 (
n
= 5; 23,80%) and CC309 (
n
= 3; 14,28%) were the most frequent. CC distribution in relation to origin revealed that three CCs were found in animals with a predominance of CC182 (66,66%), while nine were found in humans, and two CCs were found in the environment. Extensive genetic variability was observed among the isolates in the State of Mato Grosso. STs belonging to the already described clonal complexes (CC) indicate the global expansion and adaptation of isolates in several other countries. Therefore, detection of clonal complexes and STs already described in other regions and the occurrence of new STs in the present study help further the current understanding of the geographic dispersion and genetic origin of the
C. gattii
species complex.</description><identifier>ISSN: 0301-486X</identifier><identifier>EISSN: 1573-0832</identifier><identifier>DOI: 10.1007/s11046-018-0313-2</identifier><identifier>PMID: 30627957</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Analysis ; Animals ; Biodiversity ; Biomedical and Life Sciences ; Cryptococcosis ; Eukaryotic Microbiology ; Fungal infections ; Fungi ; Gene polymorphism ; Genetic diversity ; Genetic variability ; Genotypes ; Genotyping ; Global strategy (Marketing) ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbiology ; Mycoses ; Plant Sciences ; Restriction fragment length polymorphism ; Short Communication ; Species ; Species diversity</subject><ispartof>Mycopathologia (1975), 2019-02, Vol.184 (1), p.45-51</ispartof><rights>Springer Nature B.V. 2019</rights><rights>COPYRIGHT 2019 Springer</rights><rights>Mycopathologia is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c445t-2f3575d9b731ea117dfa460fae48af1cb2955b3d1a15fc8e3e1af799803115fb3</citedby><cites>FETCH-LOGICAL-c445t-2f3575d9b731ea117dfa460fae48af1cb2955b3d1a15fc8e3e1af799803115fb3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11046-018-0313-2$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11046-018-0313-2$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>315,781,785,27929,27930,41493,42562,51324</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30627957$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Maruyama, Fernanda Harumi</creatorcontrib><creatorcontrib>de Paula, Daphine Ariadne Jesus</creatorcontrib><creatorcontrib>Menezes, Isabela de Godoy</creatorcontrib><creatorcontrib>Favalessa, Olívia Cometti</creatorcontrib><creatorcontrib>Hahn, Rosane Christine</creatorcontrib><creatorcontrib>de Almeida, Arleana do Bom Parto Ferreira</creatorcontrib><creatorcontrib>Sousa, Valéria Régia Franco</creatorcontrib><creatorcontrib>Nakazato, Luciano</creatorcontrib><creatorcontrib>Dutra, Valéria</creatorcontrib><title>Genetic Diversity of the Cryptococcus gattii Species Complex in Mato Grosso State, Brazil</title><title>Mycopathologia (1975)</title><addtitle>Mycopathologia</addtitle><addtitle>Mycopathologia</addtitle><description>Cryptococcosis is caused by fungi of the genus
Cryptococcus
. Owing to its importance, this study aimed to analyze the genetic diversity of
C. gattii
isolates from animals, humans, and the environment in Mato Grosso State (MT), Brazil, during November 2010–December 2017. All isolates of the
C. gattii
species complex were subjected to molecular genotyping via Restriction Fragment Length Polymorphism (PCR–RFLP) and Multi-locus Sequence Typing (MLST). PCR–RFLP analysis revealed that 21 isolates presented the genotype VGII, which is considered the most common and virulent genotype globally among. MLST analysis revealed the presence of 14 sequence types (STs), of which 5 are considered new genotypes. Clonal Complex (CC) CC182 (
n
= 5; 23,80%) and CC309 (
n
= 3; 14,28%) were the most frequent. CC distribution in relation to origin revealed that three CCs were found in animals with a predominance of CC182 (66,66%), while nine were found in humans, and two CCs were found in the environment. Extensive genetic variability was observed among the isolates in the State of Mato Grosso. STs belonging to the already described clonal complexes (CC) indicate the global expansion and adaptation of isolates in several other countries. Therefore, detection of clonal complexes and STs already described in other regions and the occurrence of new STs in the present study help further the current understanding of the geographic dispersion and genetic origin of the
C. gattii
species complex.</description><subject>Analysis</subject><subject>Animals</subject><subject>Biodiversity</subject><subject>Biomedical and Life Sciences</subject><subject>Cryptococcosis</subject><subject>Eukaryotic Microbiology</subject><subject>Fungal infections</subject><subject>Fungi</subject><subject>Gene polymorphism</subject><subject>Genetic diversity</subject><subject>Genetic variability</subject><subject>Genotypes</subject><subject>Genotyping</subject><subject>Global strategy (Marketing)</subject><subject>Life Sciences</subject><subject>Medical Microbiology</subject><subject>Microbial Ecology</subject><subject>Microbiology</subject><subject>Mycoses</subject><subject>Plant Sciences</subject><subject>Restriction fragment length polymorphism</subject><subject>Short Communication</subject><subject>Species</subject><subject>Species diversity</subject><issn>0301-486X</issn><issn>1573-0832</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kdGL1DAQxoMo3nr6B_giAV8U7JlpmqZ9PFddD04EV0GfQppO1hzdpiap3PrXm6WncoLkITDz-4aZ7yPkMbAzYEy-jACsqgsGTcE48KK8Q1YgJC9Yw8u7ZMU4g6Jq6i8n5EGMV4xlFcj75ISzupStkCvydYMjJmfoa_cDQ3TpQL2l6RvSdThMyRtvzBzpTqfkHN1OaBxGuvb7acBr6kb6XidPN8HH6Ok26YQv6Kugf7rhIbln9RDx0c1_Sj6_ffNp_a64_LC5WJ9fFqaqRCpKy4UUfdtJDqgBZG91VTOrsWq0BdOVrRAd70GDsKZBjqCtbNsmX5wrHT8lz5a5U_DfZ4xJ7V00OAx6RD9HVYJsOYeqrTP69B_0ys9hzNtlqhaiqtuSZ-psoXZ6QOVG61PQJr8e9874Ea3L9fPsc9Vmo4-C57cEmUl4nXZ6jlFdbD_eZmFhzdGygFZNwe11OChg6hiqWkJVOVR1DFWVWfPkZu2522P_R_E7xQyUCxBza9xh-HvX_6f-As6Lqjc</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Maruyama, Fernanda Harumi</creator><creator>de Paula, Daphine Ariadne Jesus</creator><creator>Menezes, Isabela de Godoy</creator><creator>Favalessa, Olívia Cometti</creator><creator>Hahn, Rosane Christine</creator><creator>de Almeida, Arleana do Bom Parto Ferreira</creator><creator>Sousa, Valéria Régia Franco</creator><creator>Nakazato, Luciano</creator><creator>Dutra, Valéria</creator><general>Springer Netherlands</general><general>Springer</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>ISR</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope></search><sort><creationdate>20190201</creationdate><title>Genetic Diversity of the Cryptococcus gattii Species Complex in Mato Grosso State, Brazil</title><author>Maruyama, Fernanda Harumi ; 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Cryptococcus
. Owing to its importance, this study aimed to analyze the genetic diversity of
C. gattii
isolates from animals, humans, and the environment in Mato Grosso State (MT), Brazil, during November 2010–December 2017. All isolates of the
C. gattii
species complex were subjected to molecular genotyping via Restriction Fragment Length Polymorphism (PCR–RFLP) and Multi-locus Sequence Typing (MLST). PCR–RFLP analysis revealed that 21 isolates presented the genotype VGII, which is considered the most common and virulent genotype globally among. MLST analysis revealed the presence of 14 sequence types (STs), of which 5 are considered new genotypes. Clonal Complex (CC) CC182 (
n
= 5; 23,80%) and CC309 (
n
= 3; 14,28%) were the most frequent. CC distribution in relation to origin revealed that three CCs were found in animals with a predominance of CC182 (66,66%), while nine were found in humans, and two CCs were found in the environment. Extensive genetic variability was observed among the isolates in the State of Mato Grosso. STs belonging to the already described clonal complexes (CC) indicate the global expansion and adaptation of isolates in several other countries. Therefore, detection of clonal complexes and STs already described in other regions and the occurrence of new STs in the present study help further the current understanding of the geographic dispersion and genetic origin of the
C. gattii
species complex.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30627957</pmid><doi>10.1007/s11046-018-0313-2</doi><tpages>7</tpages></addata></record> |
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| subjects | Analysis Animals Biodiversity Biomedical and Life Sciences Cryptococcosis Eukaryotic Microbiology Fungal infections Fungi Gene polymorphism Genetic diversity Genetic variability Genotypes Genotyping Global strategy (Marketing) Life Sciences Medical Microbiology Microbial Ecology Microbiology Mycoses Plant Sciences Restriction fragment length polymorphism Short Communication Species Species diversity |
| title | Genetic Diversity of the Cryptococcus gattii Species Complex in Mato Grosso State, Brazil |
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