Loop assembly: a simple and open system for recursive fabrication of DNA circuits

• High-efficiency methods for DNA assembly have enabled the routine assembly of synthetic DNAs of increased size and complexity. However, these techniques require customization, elaborate vector sets or serial manipulations for the different stages of assembly. • We have developed Loop assembly base...

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Veröffentlicht in:	The New phytologist 2019-04, Vol.222 (1), p.628-640
Hauptverfasser:	Pollak, Bernardo, Cerda, Ariel, Delmans, Mihails, Álamos, Simón, Moyano, Tomás, West, Anthony, Gutiérrez, Rodrigo A., Patron, Nicola J., Federici, Fernán, Haseloff, Jim
Format:	Artikel
Sprache:	eng
Schlagworte:	Assembly Automation Circuits Cloning common syntax Construction Deoxyribonucleic acid DNA DNA sequencing DNA, Plant - genetics Fabrication Genes Genetic transformation Genetic Vectors - genetics Loop assembly LoopDesigner Marchantia - genetics Methods Nucleotide sequence Open systems OpenMTA Plasmids Plasmids - genetics Promoter Regions, Genetic - genetics recursive assembly Recursive methods Reproducibility of Results standardized DNA assembly Type IIS unique nucleotide sequences (UNSs) Vectors
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