Influence of Streptococcus mitis and Streptococcus sanguinis on virulence of Candida albicans: in vitro and in vivo studies
The aim was to evaluate in vitro possible interactions, gene expression, and biofilm formation in species of Candida albicans , Streptococcus mitis , and Streptococcus sanguinis and their in vivo pathogenicity. The in vitro analysis evaluated the effects of S. mitis and S. sanguinis on C. albicans ’...
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| Veröffentlicht in: | Folia microbiologica 2019-03, Vol.64 (2), p.215-222 |
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| creator | do Rosário Palma, Ana Luiza Domingues, Nádia de Barros, Patrícia Pimentel Brito, Graziella Nuernberg Back Jorge, Antônio Olavo Cardoso |
| description | The aim was to evaluate in vitro possible interactions, gene expression, and biofilm formation in species of
Candida albicans
,
Streptococcus mitis
, and
Streptococcus sanguinis
and their in vivo pathogenicity. The in vitro analysis evaluated the effects of
S. mitis
and
S. sanguinis
on
C. albicans
’s biofilm formation by CFU count, filamentation capacity, and adhesion (
ALS1
,
ALS3
,
HWP1
) and transcriptional regulatory gene (
BCR1
,
CPH1
,
EFG1
) expression. In vivo studies evaluated the pathogenicity of the interaction of the microorganisms on
Galleria mellonella
, with analyses of the CFU per milliliter count and filamentation. In vitro results indicated that there was an observed decrease in CFU (79.4–71.5%) in multi-species biofilms. The interaction with
S. mitis
inhibited filamentation, which seems to increase its virulence factor with over-expression of genes
ALS1
,
ALS3
, and
HWP1
as well the interaction with
S. sanguinis
as
ALS3
and
HWP1
.
S. mitis
upregulated
BRC1
,
CPH1
, and
EFG1
. The histological images of in vivo study indicate an increase in the filamentation of
C. albicans
when in interaction with the other species. It was concluded that
S. mitis
interaction suggests increased virulence factors of
C. albicans
, with periods of lower virulence and proto-cooperation in the interaction with
S. sanguinis
. |
| doi_str_mv | 10.1007/s12223-018-0645-9 |
| format | Article |
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Candida albicans
,
Streptococcus mitis
, and
Streptococcus sanguinis
and their in vivo pathogenicity. The in vitro analysis evaluated the effects of
S. mitis
and
S. sanguinis
on
C. albicans
’s biofilm formation by CFU count, filamentation capacity, and adhesion (
ALS1
,
ALS3
,
HWP1
) and transcriptional regulatory gene (
BCR1
,
CPH1
,
EFG1
) expression. In vivo studies evaluated the pathogenicity of the interaction of the microorganisms on
Galleria mellonella
, with analyses of the CFU per milliliter count and filamentation. In vitro results indicated that there was an observed decrease in CFU (79.4–71.5%) in multi-species biofilms. The interaction with
S. mitis
inhibited filamentation, which seems to increase its virulence factor with over-expression of genes
ALS1
,
ALS3
, and
HWP1
as well the interaction with
S. sanguinis
as
ALS3
and
HWP1
.
S. mitis
upregulated
BRC1
,
CPH1
, and
EFG1
. The histological images of in vivo study indicate an increase in the filamentation of
C. albicans
when in interaction with the other species. It was concluded that
S. mitis
interaction suggests increased virulence factors of
C. albicans
, with periods of lower virulence and proto-cooperation in the interaction with
S. sanguinis
.</description><identifier>ISSN: 0015-5632</identifier><identifier>EISSN: 1874-9356</identifier><identifier>DOI: 10.1007/s12223-018-0645-9</identifier><identifier>PMID: 30232727</identifier><language>eng</language><publisher>Dordrecht: Springer Netherlands</publisher><subject>Applied Microbiology ; Biofilms ; Biomedical and Life Sciences ; Candida albicans ; Environmental Engineering/Biotechnology ; Filamentation ; Gene expression ; Immunology ; In vivo methods and tests ; Life Sciences ; Microbiology ; Microorganisms ; Original Article ; Overexpression ; Pathogenicity ; Pathogens ; Species ; Streptococcus infections ; Streptococcus mitis ; Streptococcus sanguinis ; Transcription ; Virulence ; Virulence factors</subject><ispartof>Folia microbiologica, 2019-03, Vol.64 (2), p.215-222</ispartof><rights>Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i. 2018</rights><rights>Folia Microbiologica is a copyright of Springer, (2018). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-120a24d9430f96b782da512969c35cabd1743c9edabe31a4648ac1ae8485821f3</citedby><cites>FETCH-LOGICAL-c372t-120a24d9430f96b782da512969c35cabd1743c9edabe31a4648ac1ae8485821f3</cites><orcidid>0000-0001-9478-8123</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s12223-018-0645-9$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s12223-018-0645-9$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30232727$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>do Rosário Palma, Ana Luiza</creatorcontrib><creatorcontrib>Domingues, Nádia</creatorcontrib><creatorcontrib>de Barros, Patrícia Pimentel</creatorcontrib><creatorcontrib>Brito, Graziella Nuernberg Back</creatorcontrib><creatorcontrib>Jorge, Antônio Olavo Cardoso</creatorcontrib><title>Influence of Streptococcus mitis and Streptococcus sanguinis on virulence of Candida albicans: in vitro and in vivo studies</title><title>Folia microbiologica</title><addtitle>Folia Microbiol</addtitle><addtitle>Folia Microbiol (Praha)</addtitle><description>The aim was to evaluate in vitro possible interactions, gene expression, and biofilm formation in species of
Candida albicans
,
Streptococcus mitis
, and
Streptococcus sanguinis
and their in vivo pathogenicity. The in vitro analysis evaluated the effects of
S. mitis
and
S. sanguinis
on
C. albicans
’s biofilm formation by CFU count, filamentation capacity, and adhesion (
ALS1
,
ALS3
,
HWP1
) and transcriptional regulatory gene (
BCR1
,
CPH1
,
EFG1
) expression. In vivo studies evaluated the pathogenicity of the interaction of the microorganisms on
Galleria mellonella
, with analyses of the CFU per milliliter count and filamentation. In vitro results indicated that there was an observed decrease in CFU (79.4–71.5%) in multi-species biofilms. The interaction with
S. mitis
inhibited filamentation, which seems to increase its virulence factor with over-expression of genes
ALS1
,
ALS3
, and
HWP1
as well the interaction with
S. sanguinis
as
ALS3
and
HWP1
.
S. mitis
upregulated
BRC1
,
CPH1
, and
EFG1
. The histological images of in vivo study indicate an increase in the filamentation of
C. albicans
when in interaction with the other species. It was concluded that
S. mitis
interaction suggests increased virulence factors of
C. albicans
, with periods of lower virulence and proto-cooperation in the interaction with
S. sanguinis
.</description><subject>Applied Microbiology</subject><subject>Biofilms</subject><subject>Biomedical and Life Sciences</subject><subject>Candida albicans</subject><subject>Environmental Engineering/Biotechnology</subject><subject>Filamentation</subject><subject>Gene expression</subject><subject>Immunology</subject><subject>In vivo methods and tests</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Original Article</subject><subject>Overexpression</subject><subject>Pathogenicity</subject><subject>Pathogens</subject><subject>Species</subject><subject>Streptococcus infections</subject><subject>Streptococcus mitis</subject><subject>Streptococcus sanguinis</subject><subject>Transcription</subject><subject>Virulence</subject><subject>Virulence 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of Streptococcus mitis and Streptococcus sanguinis on virulence of Candida albicans: in vitro and in vivo studies</title><author>do Rosário Palma, Ana Luiza ; Domingues, Nádia ; de Barros, Patrícia Pimentel ; Brito, Graziella Nuernberg Back ; Jorge, Antônio Olavo Cardoso</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-120a24d9430f96b782da512969c35cabd1743c9edabe31a4648ac1ae8485821f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Applied Microbiology</topic><topic>Biofilms</topic><topic>Biomedical and Life Sciences</topic><topic>Candida albicans</topic><topic>Environmental Engineering/Biotechnology</topic><topic>Filamentation</topic><topic>Gene expression</topic><topic>Immunology</topic><topic>In vivo methods and tests</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Original Article</topic><topic>Overexpression</topic><topic>Pathogenicity</topic><topic>Pathogens</topic><topic>Species</topic><topic>Streptococcus infections</topic><topic>Streptococcus mitis</topic><topic>Streptococcus sanguinis</topic><topic>Transcription</topic><topic>Virulence</topic><topic>Virulence factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>do Rosário Palma, Ana Luiza</creatorcontrib><creatorcontrib>Domingues, Nádia</creatorcontrib><creatorcontrib>de Barros, Patrícia Pimentel</creatorcontrib><creatorcontrib>Brito, Graziella Nuernberg Back</creatorcontrib><creatorcontrib>Jorge, Antônio Olavo Cardoso</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS 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(Praha)</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>64</volume><issue>2</issue><spage>215</spage><epage>222</epage><pages>215-222</pages><issn>0015-5632</issn><eissn>1874-9356</eissn><abstract>The aim was to evaluate in vitro possible interactions, gene expression, and biofilm formation in species of
Candida albicans
,
Streptococcus mitis
, and
Streptococcus sanguinis
and their in vivo pathogenicity. The in vitro analysis evaluated the effects of
S. mitis
and
S. sanguinis
on
C. albicans
’s biofilm formation by CFU count, filamentation capacity, and adhesion (
ALS1
,
ALS3
,
HWP1
) and transcriptional regulatory gene (
BCR1
,
CPH1
,
EFG1
) expression. In vivo studies evaluated the pathogenicity of the interaction of the microorganisms on
Galleria mellonella
, with analyses of the CFU per milliliter count and filamentation. In vitro results indicated that there was an observed decrease in CFU (79.4–71.5%) in multi-species biofilms. The interaction with
S. mitis
inhibited filamentation, which seems to increase its virulence factor with over-expression of genes
ALS1
,
ALS3
, and
HWP1
as well the interaction with
S. sanguinis
as
ALS3
and
HWP1
.
S. mitis
upregulated
BRC1
,
CPH1
, and
EFG1
. The histological images of in vivo study indicate an increase in the filamentation of
C. albicans
when in interaction with the other species. It was concluded that
S. mitis
interaction suggests increased virulence factors of
C. albicans
, with periods of lower virulence and proto-cooperation in the interaction with
S. sanguinis
.</abstract><cop>Dordrecht</cop><pub>Springer Netherlands</pub><pmid>30232727</pmid><doi>10.1007/s12223-018-0645-9</doi><tpages>8</tpages><orcidid>https://orcid.org/0000-0001-9478-8123</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0015-5632 |
| ispartof | Folia microbiologica, 2019-03, Vol.64 (2), p.215-222 |
| issn | 0015-5632 1874-9356 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2179218792 |
| source | SpringerNature Journals |
| subjects | Applied Microbiology Biofilms Biomedical and Life Sciences Candida albicans Environmental Engineering/Biotechnology Filamentation Gene expression Immunology In vivo methods and tests Life Sciences Microbiology Microorganisms Original Article Overexpression Pathogenicity Pathogens Species Streptococcus infections Streptococcus mitis Streptococcus sanguinis Transcription Virulence Virulence factors |
| title | Influence of Streptococcus mitis and Streptococcus sanguinis on virulence of Candida albicans: in vitro and in vivo studies |
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