Rapid Detection of Avian Influenza A Virus (H7N9) by Lateral Flow Dipstick Recombinase Polymerase Amplification
Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2018/12/01, Vol.41(12), pp.1804-1808 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | Ma, Shiwei Li, Xue Peng, Bo Wu, Weihua Wang, Xin Liu, Hui Yuan, Lihong Fang, Shisong Lu, Jiahai |
| description | Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time RT-PCR assay. The results data demonstrated that our method performed well and could be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9. |
| doi_str_mv | 10.1248/bpb.b18-00468 |
| format | Article |
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With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time RT-PCR assay. The results data demonstrated that our method performed well and could be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.b18-00468</identifier><identifier>PMID: 30232304</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Assaying ; Avian flu ; avian influenza A virus ; Birds ; Cross-reaction ; Diagnosis ; DNA-Directed RNA Polymerases - analysis ; DNA-Directed RNA Polymerases - genetics ; Epidemics ; Exo-a-sialidase ; H7N9 ; Hemagglutinins ; Humans ; Influenza ; Influenza A ; Influenza A Virus, H7N9 Subtype - genetics ; Influenza A Virus, H7N9 Subtype - isolation & purification ; Influenza in Birds - genetics ; lateral flow dipstick ; Nucleic Acid Amplification Techniques - methods ; Polymerase chain reaction ; Poultry ; Real-Time Polymerase Chain Reaction - methods ; Recombinase ; recombinase polymerase amplification ; Recombinases - genetics ; Time Factors ; Viruses</subject><ispartof>Biological and Pharmaceutical Bulletin, 2018/12/01, Vol.41(12), pp.1804-1808</ispartof><rights>2018 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2018</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c679t-3de6d3a4a24beba4f2b75d9ebe0052accd830f409fb166f3e88536e413d3b4f73</citedby><cites>FETCH-LOGICAL-c679t-3de6d3a4a24beba4f2b75d9ebe0052accd830f409fb166f3e88536e413d3b4f73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30232304$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ma, Shiwei</creatorcontrib><creatorcontrib>Li, Xue</creatorcontrib><creatorcontrib>Peng, Bo</creatorcontrib><creatorcontrib>Wu, Weihua</creatorcontrib><creatorcontrib>Wang, Xin</creatorcontrib><creatorcontrib>Liu, Hui</creatorcontrib><creatorcontrib>Yuan, Lihong</creatorcontrib><creatorcontrib>Fang, Shisong</creatorcontrib><creatorcontrib>Lu, Jiahai</creatorcontrib><creatorcontrib>Sun Yat-sen University</creatorcontrib><creatorcontrib>Key Reference Laboratory of Pathogen and Biosafety</creatorcontrib><creatorcontrib>Shenzhen Center</creatorcontrib><creatorcontrib>bMajor Infectious Disease Control Key Laboratory</creatorcontrib><creatorcontrib>for Disease Control and Prevention</creatorcontrib><creatorcontrib>aSchool of Public Health</creatorcontrib><title>Rapid Detection of Avian Influenza A Virus (H7N9) by Lateral Flow Dipstick Recombinase Polymerase Amplification</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Avian influenza A (H7N9) virus has caused several epidemics and infection in both human and poultry. With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time RT-PCR assay. The results data demonstrated that our method performed well and could be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9.</description><subject>Animals</subject><subject>Assaying</subject><subject>Avian flu</subject><subject>avian influenza A virus</subject><subject>Birds</subject><subject>Cross-reaction</subject><subject>Diagnosis</subject><subject>DNA-Directed RNA Polymerases - analysis</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Epidemics</subject><subject>Exo-a-sialidase</subject><subject>H7N9</subject><subject>Hemagglutinins</subject><subject>Humans</subject><subject>Influenza</subject><subject>Influenza A</subject><subject>Influenza A Virus, H7N9 Subtype - genetics</subject><subject>Influenza A Virus, H7N9 Subtype - isolation & purification</subject><subject>Influenza in Birds - genetics</subject><subject>lateral flow dipstick</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Polymerase chain reaction</subject><subject>Poultry</subject><subject>Real-Time Polymerase Chain Reaction - methods</subject><subject>Recombinase</subject><subject>recombinase polymerase amplification</subject><subject>Recombinases - genetics</subject><subject>Time Factors</subject><subject>Viruses</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkc1v1DAQxSMEotvCkSuyxKU9pPgriXNcdemHtAJUAVfLdsbgxbFDnICWvx5vUxYJyZ6xND-9efIrilcEXxLKxVs96EtNRIkxr8WTYkUYb8qKkuppscJtHtSkEifFaUo7jHGDKXtenLDcKMN8VcR7NbgObWACM7kYULRo_dOpgO6C9TOE3wqt0Rc3zgmd3zbv2wuk92irJhiVR9c-_kIbN6TJme_oHkzstQsqAfoY_b7PTH6u-8E764w66L8onlnlE7x87GfF5-t3n65uy-2Hm7ur9bY0ddNOJeug7pjiinINWnFLdVN1LWjAuKLKmE4wbDlurSZ1bRkIUbEaOGEd09w27Kw4X3SHMf6YIU2yd8mA9ypAnJOkpGnzFbjN6Jv_0F2cx5DdSUp5PjURdabKhTJjTGkEK4fR9WrcS4LlIQmZk5A5CfmQROZfP6rOuofuSP_9-gzcLECe5s_xMXgX4N9ukxrtoo-S4kWUEJpbK4nA_FAEIyJXmpU2i9IuTeorHFepMcfi4cEYJ9nkoR4dHsfmmxolBPYHMdGx8w</recordid><startdate>20181201</startdate><enddate>20181201</enddate><creator>Ma, Shiwei</creator><creator>Li, Xue</creator><creator>Peng, Bo</creator><creator>Wu, Weihua</creator><creator>Wang, Xin</creator><creator>Liu, Hui</creator><creator>Yuan, Lihong</creator><creator>Fang, Shisong</creator><creator>Lu, Jiahai</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20181201</creationdate><title>Rapid Detection of Avian Influenza A Virus (H7N9) by Lateral Flow Dipstick Recombinase Polymerase Amplification</title><author>Ma, Shiwei ; 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With mutation, the H7N9 virus gained its fifth endemic in China. Early diagnosis is crucial for the control of viral spread in poultry and prognosis of infected patients. In this study, we developed and evaluated a lateral flow dipstick recombinase polymerase amplification (LFD-RPA) assay for rapid detection of both hemagglutinin and neuraminidase gene of H7N9. Our H7-LFD-RPA and N9-LFD-RPA assay were able to detect 32 fg H7N9 nucleic acid which is more convenient and rapid than previous methods. Through detecting 50 influenza positive samples, cross-reaction was not found with other subtypes of influenza virus. The 100% analytical specificity and sufficient analytical sensitivity results agreed the real time RT-PCR assay. The results data demonstrated that our method performed well and could be applied to the detection of H7N9 virus. This LFD-RPA assay provides a candidate method for rapid point-of-care diagnosis of H7N9.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>30232304</pmid><doi>10.1248/bpb.b18-00468</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Assaying Avian flu avian influenza A virus Birds Cross-reaction Diagnosis DNA-Directed RNA Polymerases - analysis DNA-Directed RNA Polymerases - genetics Epidemics Exo-a-sialidase H7N9 Hemagglutinins Humans Influenza Influenza A Influenza A Virus, H7N9 Subtype - genetics Influenza A Virus, H7N9 Subtype - isolation & purification Influenza in Birds - genetics lateral flow dipstick Nucleic Acid Amplification Techniques - methods Polymerase chain reaction Poultry Real-Time Polymerase Chain Reaction - methods Recombinase recombinase polymerase amplification Recombinases - genetics Time Factors Viruses |
| title | Rapid Detection of Avian Influenza A Virus (H7N9) by Lateral Flow Dipstick Recombinase Polymerase Amplification |
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