Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state
Species of the genus Anaplasma, in the family Anaplasmatacae, are responsible to vector-borne diseases that affecting animals and humans. Feline anaplasmosis is poorly reported in Brazil. This study aimed at investigating the occurrence of Anaplasma spp. in domestic cats from Greater Rio de Janeiro,...
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| Veröffentlicht in: | Acta tropica 2019-03, Vol.191, p.239-242 |
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| creator | Guimarães, Andresa Raimundo, Juliana Macedo Peixoto, Maristela Peckle da Silva, Claudia Bezerra Pires, Marcus Sandes Santos, Huarrisson Azevedo Baldani, Cristiane Divan |
| description | Species of the genus Anaplasma, in the family Anaplasmatacae, are responsible to vector-borne diseases that affecting animals and humans. Feline anaplasmosis is poorly reported in Brazil. This study aimed at investigating the occurrence of Anaplasma spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. Were sampled 216 cats, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting A. platys-16S-rDNA, A. platys-gltA and A. phagocytophilum-msp2 sequences. As evaluated with gltA-qPCR the frequency of cats positive for A. platys was 3.7% (n = 8/216) and by 16S-rDNA nested-PCR it was 0.9% (n = 2/216). No cats were positive to msp2-qPCR to A. phagocytophilum. The sequences of A. platys presented 100% similarity with previously described isolates around the world and Brazil. Two cats that were positive in the gltA-qPCR reactions have platelet inclusions in the microscopic examination. However, no significant (p > 0.05) hematological changes were observed, probably due to low parasite load. This study showed that A. platys occur in domestic cats from Greater Rio de Janeiro. Further studies are needed to more precisely characterize these organisms. |
| doi_str_mv | 10.1016/j.actatropica.2019.01.003 |
| format | Article |
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Feline anaplasmosis is poorly reported in Brazil. This study aimed at investigating the occurrence of Anaplasma spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. Were sampled 216 cats, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting A. platys-16S-rDNA, A. platys-gltA and A. phagocytophilum-msp2 sequences. As evaluated with gltA-qPCR the frequency of cats positive for A. platys was 3.7% (n = 8/216) and by 16S-rDNA nested-PCR it was 0.9% (n = 2/216). No cats were positive to msp2-qPCR to A. phagocytophilum. The sequences of A. platys presented 100% similarity with previously described isolates around the world and Brazil. Two cats that were positive in the gltA-qPCR reactions have platelet inclusions in the microscopic examination. However, no significant (p > 0.05) hematological changes were observed, probably due to low parasite load. This study showed that A. platys occur in domestic cats from Greater Rio de Janeiro. Further studies are needed to more precisely characterize these organisms.</description><identifier>ISSN: 0001-706X</identifier><identifier>EISSN: 1873-6254</identifier><identifier>DOI: 10.1016/j.actatropica.2019.01.003</identifier><identifier>PMID: 30615856</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Anaplasma - genetics ; Anaplasma - isolation & purification ; Anaplasma platys ; Anaplasmosis - epidemiology ; Anaplasmosis - genetics ; Animals ; Brazil - epidemiology ; Cat Diseases - epidemiology ; Cat Diseases - genetics ; Cats ; DNA, Ribosomal ; Dogs ; Domestic felines ; Greater Rio de Janeiro ; Hematological changes ; Humans ; PCR ; Real-Time Polymerase Chain Reaction</subject><ispartof>Acta tropica, 2019-03, Vol.191, p.239-242</ispartof><rights>2019 Elsevier B.V.</rights><rights>Copyright © 2019 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c377t-c560613b53d610644dfbcef0546ac26b89cda1d9d7ca617e9a3e1507c5cf4db23</citedby><cites>FETCH-LOGICAL-c377t-c560613b53d610644dfbcef0546ac26b89cda1d9d7ca617e9a3e1507c5cf4db23</cites><orcidid>0000-0003-1761-2110</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0001706X18313998$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30615856$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guimarães, Andresa</creatorcontrib><creatorcontrib>Raimundo, Juliana Macedo</creatorcontrib><creatorcontrib>Peixoto, Maristela Peckle</creatorcontrib><creatorcontrib>da Silva, Claudia Bezerra</creatorcontrib><creatorcontrib>Pires, Marcus Sandes</creatorcontrib><creatorcontrib>Santos, Huarrisson Azevedo</creatorcontrib><creatorcontrib>Baldani, Cristiane Divan</creatorcontrib><title>Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state</title><title>Acta tropica</title><addtitle>Acta Trop</addtitle><description>Species of the genus Anaplasma, in the family Anaplasmatacae, are responsible to vector-borne diseases that affecting animals and humans. Feline anaplasmosis is poorly reported in Brazil. This study aimed at investigating the occurrence of Anaplasma spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. Were sampled 216 cats, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting A. platys-16S-rDNA, A. platys-gltA and A. phagocytophilum-msp2 sequences. As evaluated with gltA-qPCR the frequency of cats positive for A. platys was 3.7% (n = 8/216) and by 16S-rDNA nested-PCR it was 0.9% (n = 2/216). No cats were positive to msp2-qPCR to A. phagocytophilum. The sequences of A. platys presented 100% similarity with previously described isolates around the world and Brazil. Two cats that were positive in the gltA-qPCR reactions have platelet inclusions in the microscopic examination. However, no significant (p > 0.05) hematological changes were observed, probably due to low parasite load. This study showed that A. platys occur in domestic cats from Greater Rio de Janeiro. Further studies are needed to more precisely characterize these organisms.</description><subject>Anaplasma - genetics</subject><subject>Anaplasma - isolation & purification</subject><subject>Anaplasma platys</subject><subject>Anaplasmosis - epidemiology</subject><subject>Anaplasmosis - genetics</subject><subject>Animals</subject><subject>Brazil - epidemiology</subject><subject>Cat Diseases - epidemiology</subject><subject>Cat Diseases - genetics</subject><subject>Cats</subject><subject>DNA, Ribosomal</subject><subject>Dogs</subject><subject>Domestic felines</subject><subject>Greater Rio de Janeiro</subject><subject>Hematological changes</subject><subject>Humans</subject><subject>PCR</subject><subject>Real-Time Polymerase Chain Reaction</subject><issn>0001-706X</issn><issn>1873-6254</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE9r3DAQxUVpabZpv0JQbj3U7si25PUxLP1LSiCk0JsYj8ZUi205krfQfvpo2aT02NMww5t5b35CXCooFSjzfl8irbjGsHjCsgLVlaBKgPqZ2KhtWxem0s1zsQEAVbRgfpyJVyntc1e1unopzmowSm-12Yj-WxiZDiNG6XhlWn2Y30n6iTFbcPR_8DiRYZBXMy4jpgllWpZS-lm6MHFaPUnCNckhhkne-pDvyK84s49BppySX4sXA46J3zzWc_H944e73efi-ubTl93VdUF1264FaZNT1b2unVFgmsYNPfEAujFIlem3HTlUrnMtoVEtd1iz0tCSpqFxfVWfi7enu0sM94eczE4-EY9jDhMOyVbKaGiU2kKWdicpxZBS5MEu0U8Yf1sF9ojY7u0_iO0RsQVlM-K8e_Foc-gndn83n5hmwe4k4PzsL8_RJvI8EzsfM2Drgv8PmwdfWJQt</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Guimarães, Andresa</creator><creator>Raimundo, Juliana Macedo</creator><creator>Peixoto, Maristela Peckle</creator><creator>da Silva, Claudia Bezerra</creator><creator>Pires, Marcus Sandes</creator><creator>Santos, Huarrisson Azevedo</creator><creator>Baldani, Cristiane Divan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-1761-2110</orcidid></search><sort><creationdate>201903</creationdate><title>Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state</title><author>Guimarães, Andresa ; Raimundo, Juliana Macedo ; Peixoto, Maristela Peckle ; da Silva, Claudia Bezerra ; Pires, Marcus Sandes ; Santos, Huarrisson Azevedo ; Baldani, Cristiane Divan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c377t-c560613b53d610644dfbcef0546ac26b89cda1d9d7ca617e9a3e1507c5cf4db23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anaplasma - genetics</topic><topic>Anaplasma - isolation & purification</topic><topic>Anaplasma platys</topic><topic>Anaplasmosis - epidemiology</topic><topic>Anaplasmosis - genetics</topic><topic>Animals</topic><topic>Brazil - epidemiology</topic><topic>Cat Diseases - epidemiology</topic><topic>Cat Diseases - genetics</topic><topic>Cats</topic><topic>DNA, Ribosomal</topic><topic>Dogs</topic><topic>Domestic felines</topic><topic>Greater Rio de Janeiro</topic><topic>Hematological changes</topic><topic>Humans</topic><topic>PCR</topic><topic>Real-Time Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guimarães, Andresa</creatorcontrib><creatorcontrib>Raimundo, Juliana Macedo</creatorcontrib><creatorcontrib>Peixoto, Maristela Peckle</creatorcontrib><creatorcontrib>da Silva, Claudia Bezerra</creatorcontrib><creatorcontrib>Pires, Marcus Sandes</creatorcontrib><creatorcontrib>Santos, Huarrisson Azevedo</creatorcontrib><creatorcontrib>Baldani, Cristiane Divan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Acta tropica</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guimarães, Andresa</au><au>Raimundo, Juliana Macedo</au><au>Peixoto, Maristela Peckle</au><au>da Silva, Claudia Bezerra</au><au>Pires, Marcus Sandes</au><au>Santos, Huarrisson Azevedo</au><au>Baldani, Cristiane Divan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state</atitle><jtitle>Acta tropica</jtitle><addtitle>Acta Trop</addtitle><date>2019-03</date><risdate>2019</risdate><volume>191</volume><spage>239</spage><epage>242</epage><pages>239-242</pages><issn>0001-706X</issn><eissn>1873-6254</eissn><abstract>Species of the genus Anaplasma, in the family Anaplasmatacae, are responsible to vector-borne diseases that affecting animals and humans. Feline anaplasmosis is poorly reported in Brazil. This study aimed at investigating the occurrence of Anaplasma spp. in domestic cats from Greater Rio de Janeiro, and evaluating hematological changes associated with this rickettsial infection. Were sampled 216 cats, we performed nested PCR (nPCR) and real-time PCR (qPCR) assays targeting A. platys-16S-rDNA, A. platys-gltA and A. phagocytophilum-msp2 sequences. As evaluated with gltA-qPCR the frequency of cats positive for A. platys was 3.7% (n = 8/216) and by 16S-rDNA nested-PCR it was 0.9% (n = 2/216). No cats were positive to msp2-qPCR to A. phagocytophilum. The sequences of A. platys presented 100% similarity with previously described isolates around the world and Brazil. Two cats that were positive in the gltA-qPCR reactions have platelet inclusions in the microscopic examination. However, no significant (p > 0.05) hematological changes were observed, probably due to low parasite load. This study showed that A. platys occur in domestic cats from Greater Rio de Janeiro. Further studies are needed to more precisely characterize these organisms.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30615856</pmid><doi>10.1016/j.actatropica.2019.01.003</doi><tpages>4</tpages><orcidid>https://orcid.org/0000-0003-1761-2110</orcidid></addata></record> |
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| subjects | Anaplasma - genetics Anaplasma - isolation & purification Anaplasma platys Anaplasmosis - epidemiology Anaplasmosis - genetics Animals Brazil - epidemiology Cat Diseases - epidemiology Cat Diseases - genetics Cats DNA, Ribosomal Dogs Domestic felines Greater Rio de Janeiro Hematological changes Humans PCR Real-Time Polymerase Chain Reaction |
| title | Molecular detection, characterization of Anaplasma spp. in domestic cats from Rio de Janeiro state |
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