The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries
Objective This study investigated the ability of hypoxia‐induced 25‐fold concentrated conditioned media (hCM) from human nasal inferior turbinate‐derived mesenchymal stem cells (hTMSC) to repair injured vocal folds during the early phase of the wound‐healing process. Methods The vocal fold was injur...
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| Veröffentlicht in: | The Laryngoscope 2019-08, Vol.129 (8), p.1867-1875 |
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| container_title | The Laryngoscope |
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| creator | Kim, Choung‐Soo Choi, Hyunsu Kim, Sung Won Sun, Dong‐Il |
| description | Objective
This study investigated the ability of hypoxia‐induced 25‐fold concentrated conditioned media (hCM) from human nasal inferior turbinate‐derived mesenchymal stem cells (hTMSC) to repair injured vocal folds during the early phase of the wound‐healing process.
Methods
The vocal fold was injured in Sprague‐Dawley rats. Next, hCM from hTMSC (the hCM group) or hTMSC (the hTMSC group) were injected into the injured vocal folds. As a control, saline (the phosphate‐buffered saline group) or 25‐fold concentrated media (the media group) was injected in the same manner. The vocal folds were harvested for quantitative real‐time polymerase chain reaction (PCR) at 1 week and 2 weeks after injury. Histologic evaluation was performed at 3 weeks postinjury.
Results
In the hCM group at 1 week after injury, PCR showed that the genes encoding hyaluronan synthase (HAS), HAS 1, and HAS 2 were significantly upregulated compared to the media and normal groups. The gene encoding procollagen III was significantly downregulated compared to the media group. Nearly identical results were obtained for the hTMSC group at 1 week after injury. Histological examination showed that the hCM group was similar to or better than the hTMSC group in collagen deposition and hyaluronic acid production.
Conclusion
The injection of hCM into injured vocal folds produced antifibrotic effects in the early phase of wound healing. These effects were equivalent to those produced by the injection of hTMSC. These results provide a foundation for the future clinical use of hCM for vocal fold regeneration.
Level of Evidence
NA
Laryngoscope, 129:1867–1875, 2019 |
| doi_str_mv | 10.1002/lary.27679 |
| format | Article |
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This study investigated the ability of hypoxia‐induced 25‐fold concentrated conditioned media (hCM) from human nasal inferior turbinate‐derived mesenchymal stem cells (hTMSC) to repair injured vocal folds during the early phase of the wound‐healing process.
Methods
The vocal fold was injured in Sprague‐Dawley rats. Next, hCM from hTMSC (the hCM group) or hTMSC (the hTMSC group) were injected into the injured vocal folds. As a control, saline (the phosphate‐buffered saline group) or 25‐fold concentrated media (the media group) was injected in the same manner. The vocal folds were harvested for quantitative real‐time polymerase chain reaction (PCR) at 1 week and 2 weeks after injury. Histologic evaluation was performed at 3 weeks postinjury.
Results
In the hCM group at 1 week after injury, PCR showed that the genes encoding hyaluronan synthase (HAS), HAS 1, and HAS 2 were significantly upregulated compared to the media and normal groups. The gene encoding procollagen III was significantly downregulated compared to the media group. Nearly identical results were obtained for the hTMSC group at 1 week after injury. Histological examination showed that the hCM group was similar to or better than the hTMSC group in collagen deposition and hyaluronic acid production.
Conclusion
The injection of hCM into injured vocal folds produced antifibrotic effects in the early phase of wound healing. These effects were equivalent to those produced by the injection of hTMSC. These results provide a foundation for the future clinical use of hCM for vocal fold regeneration.
Level of Evidence
NA
Laryngoscope, 129:1867–1875, 2019</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1002/lary.27679</identifier><identifier>PMID: 30613969</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Conditioned media ; Hyaluronic acid ; mesenchymal stem cell ; Stem cells ; vocal fold ; wound healing</subject><ispartof>The Laryngoscope, 2019-08, Vol.129 (8), p.1867-1875</ispartof><rights>2019 The American Laryngological, Rhinological and Otological Society, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3579-25c39f92617c7aad982ff82ecccbc988a660516f28e455dd02a537a39db2ff493</citedby><cites>FETCH-LOGICAL-c3579-25c39f92617c7aad982ff82ecccbc988a660516f28e455dd02a537a39db2ff493</cites><orcidid>0000-0002-8981-2536 ; 0000-0002-9551-9571 ; 0000-0001-9950-568X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flary.27679$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flary.27679$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30613969$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Choung‐Soo</creatorcontrib><creatorcontrib>Choi, Hyunsu</creatorcontrib><creatorcontrib>Kim, Sung Won</creatorcontrib><creatorcontrib>Sun, Dong‐Il</creatorcontrib><title>The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries</title><title>The Laryngoscope</title><addtitle>Laryngoscope</addtitle><description>Objective
This study investigated the ability of hypoxia‐induced 25‐fold concentrated conditioned media (hCM) from human nasal inferior turbinate‐derived mesenchymal stem cells (hTMSC) to repair injured vocal folds during the early phase of the wound‐healing process.
Methods
The vocal fold was injured in Sprague‐Dawley rats. Next, hCM from hTMSC (the hCM group) or hTMSC (the hTMSC group) were injected into the injured vocal folds. As a control, saline (the phosphate‐buffered saline group) or 25‐fold concentrated media (the media group) was injected in the same manner. The vocal folds were harvested for quantitative real‐time polymerase chain reaction (PCR) at 1 week and 2 weeks after injury. Histologic evaluation was performed at 3 weeks postinjury.
Results
In the hCM group at 1 week after injury, PCR showed that the genes encoding hyaluronan synthase (HAS), HAS 1, and HAS 2 were significantly upregulated compared to the media and normal groups. The gene encoding procollagen III was significantly downregulated compared to the media group. Nearly identical results were obtained for the hTMSC group at 1 week after injury. Histological examination showed that the hCM group was similar to or better than the hTMSC group in collagen deposition and hyaluronic acid production.
Conclusion
The injection of hCM into injured vocal folds produced antifibrotic effects in the early phase of wound healing. These effects were equivalent to those produced by the injection of hTMSC. These results provide a foundation for the future clinical use of hCM for vocal fold regeneration.
Level of Evidence
NA
Laryngoscope, 129:1867–1875, 2019</description><subject>Conditioned media</subject><subject>Hyaluronic acid</subject><subject>mesenchymal stem cell</subject><subject>Stem cells</subject><subject>vocal fold</subject><subject>wound healing</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kM1LwzAYh4Mobn5c_AMk4EWEznw0aXMcw-lgKswpegpZkmJG28ykRfbf27npwYOn9_A-PPx4ADjDaIARItelCusByXgm9kAfM4qTVAi2D_rdkyY5I689cBTjEiGcUYYOQY8ijqngog8e5u8WDheudM0a-gKOfG1c43xtDby3xik4Dr6CT42t4MiWZYSNhzO7Ui7AF69VCce-NHBSL9vgbDwBB4Uqoz3d3WPwPL6Zj-6S6ePtZDScJpqyTCSEaSoKQTjOdKaUETkpipxYrfVCizxXnCOGeUFymzJmDCKK0UxRYRYdmAp6DC633lXwH62Njaxc1N0-VVvfRkkwT1nKRUY69OIPuvRtqLt1khAmCKOcboRXW0oHH2OwhVwFV3VhJUZyU1luKsvvyh18vlO2i8qaX_QnawfgLfDpSrv-RyWnw9nbVvoFRwKFww</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Kim, Choung‐Soo</creator><creator>Choi, Hyunsu</creator><creator>Kim, Sung Won</creator><creator>Sun, Dong‐Il</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8981-2536</orcidid><orcidid>https://orcid.org/0000-0002-9551-9571</orcidid><orcidid>https://orcid.org/0000-0001-9950-568X</orcidid></search><sort><creationdate>201908</creationdate><title>The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries</title><author>Kim, Choung‐Soo ; Choi, Hyunsu ; Kim, Sung Won ; Sun, Dong‐Il</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3579-25c39f92617c7aad982ff82ecccbc988a660516f28e455dd02a537a39db2ff493</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Conditioned media</topic><topic>Hyaluronic acid</topic><topic>mesenchymal stem cell</topic><topic>Stem cells</topic><topic>vocal fold</topic><topic>wound healing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Choung‐Soo</creatorcontrib><creatorcontrib>Choi, Hyunsu</creatorcontrib><creatorcontrib>Kim, Sung Won</creatorcontrib><creatorcontrib>Sun, Dong‐Il</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Choung‐Soo</au><au>Choi, Hyunsu</au><au>Kim, Sung Won</au><au>Sun, Dong‐Il</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries</atitle><jtitle>The Laryngoscope</jtitle><addtitle>Laryngoscope</addtitle><date>2019-08</date><risdate>2019</risdate><volume>129</volume><issue>8</issue><spage>1867</spage><epage>1875</epage><pages>1867-1875</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><abstract>Objective
This study investigated the ability of hypoxia‐induced 25‐fold concentrated conditioned media (hCM) from human nasal inferior turbinate‐derived mesenchymal stem cells (hTMSC) to repair injured vocal folds during the early phase of the wound‐healing process.
Methods
The vocal fold was injured in Sprague‐Dawley rats. Next, hCM from hTMSC (the hCM group) or hTMSC (the hTMSC group) were injected into the injured vocal folds. As a control, saline (the phosphate‐buffered saline group) or 25‐fold concentrated media (the media group) was injected in the same manner. The vocal folds were harvested for quantitative real‐time polymerase chain reaction (PCR) at 1 week and 2 weeks after injury. Histologic evaluation was performed at 3 weeks postinjury.
Results
In the hCM group at 1 week after injury, PCR showed that the genes encoding hyaluronan synthase (HAS), HAS 1, and HAS 2 were significantly upregulated compared to the media and normal groups. The gene encoding procollagen III was significantly downregulated compared to the media group. Nearly identical results were obtained for the hTMSC group at 1 week after injury. Histological examination showed that the hCM group was similar to or better than the hTMSC group in collagen deposition and hyaluronic acid production.
Conclusion
The injection of hCM into injured vocal folds produced antifibrotic effects in the early phase of wound healing. These effects were equivalent to those produced by the injection of hTMSC. These results provide a foundation for the future clinical use of hCM for vocal fold regeneration.
Level of Evidence
NA
Laryngoscope, 129:1867–1875, 2019</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30613969</pmid><doi>10.1002/lary.27679</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0002-8981-2536</orcidid><orcidid>https://orcid.org/0000-0002-9551-9571</orcidid><orcidid>https://orcid.org/0000-0001-9950-568X</orcidid></addata></record> |
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| ispartof | The Laryngoscope, 2019-08, Vol.129 (8), p.1867-1875 |
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| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Conditioned media Hyaluronic acid mesenchymal stem cell Stem cells vocal fold wound healing |
| title | The Ability of Conditioned Media From Stem Cells to Repair Vocal Fold Injuries |
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