In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination
Contents This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Ne...
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| Veröffentlicht in: | Reproduction in domestic animals 2019-03, Vol.54 (3), p.604-612 |
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| creator | Okano, Denise S. Penitente‐Filho, Jurandy M. Gomez León, Victor E. Maitan, Paula P. Silveira, Camila O. Waddington, Bruna Díaz‐Miranda, Edgar A. da Costa, Eduardo P. Guimarães, Simone E. F. Guimarães, José D. |
| description | Contents
This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows. |
| doi_str_mv | 10.1111/rda.13401 |
| format | Article |
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This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/rda.13401</identifier><identifier>PMID: 30614080</identifier><language>eng</language><publisher>Germany: Blackwell Publishing Ltd</publisher><subject>Artificial insemination ; Capacitation ; Cattle ; Cryopreservation ; Fertility ; flow cytometer ; Hydrogen peroxide ; Injury analysis ; Integrity ; Membranes ; Motility ; Multivariate analysis ; Pregnancy ; Regression analysis ; Reproduction (biology) ; Semen ; semen fertility ; Sperm ; Statistical analysis ; Thawing</subject><ispartof>Reproduction in domestic animals, 2019-03, Vol.54 (3), p.604-612</ispartof><rights>2019 Blackwell Verlag GmbH</rights><rights>2019 Blackwell Verlag GmbH.</rights><rights>Copyright © 2019 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3881-e37d72f864670241665a1e9723ede617294cc09d5e7d08a8e50153ec3a5e97fe3</citedby><cites>FETCH-LOGICAL-c3881-e37d72f864670241665a1e9723ede617294cc09d5e7d08a8e50153ec3a5e97fe3</cites><orcidid>0000-0002-8592-4020 ; 0000-0001-6677-9609</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Frda.13401$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Frda.13401$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30614080$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Okano, Denise S.</creatorcontrib><creatorcontrib>Penitente‐Filho, Jurandy M.</creatorcontrib><creatorcontrib>Gomez León, Victor E.</creatorcontrib><creatorcontrib>Maitan, Paula P.</creatorcontrib><creatorcontrib>Silveira, Camila O.</creatorcontrib><creatorcontrib>Waddington, Bruna</creatorcontrib><creatorcontrib>Díaz‐Miranda, Edgar A.</creatorcontrib><creatorcontrib>da Costa, Eduardo P.</creatorcontrib><creatorcontrib>Guimarães, Simone E. F.</creatorcontrib><creatorcontrib>Guimarães, José D.</creatorcontrib><title>In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows.</description><subject>Artificial insemination</subject><subject>Capacitation</subject><subject>Cattle</subject><subject>Cryopreservation</subject><subject>Fertility</subject><subject>flow cytometer</subject><subject>Hydrogen peroxide</subject><subject>Injury analysis</subject><subject>Integrity</subject><subject>Membranes</subject><subject>Motility</subject><subject>Multivariate analysis</subject><subject>Pregnancy</subject><subject>Regression analysis</subject><subject>Reproduction (biology)</subject><subject>Semen</subject><subject>semen fertility</subject><subject>Sperm</subject><subject>Statistical analysis</subject><subject>Thawing</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp10ctKxDAUBuAgijNeFr6ABNzoojNJ0ybpcvA6IAii6xKbU8iQNmPSjs5C8BF8Rp_EaNWFYDaBnC8_CT9CB5RMaFxTr9WEsozQDTSmGSsSkjO6icakYDzhgssR2glhQQjNpRDbaMQIpxmRZIxe5i1emc47DCtle9UZ12JX48qv3dJDAL8CjR_cyrSAwxJ8g1WrsekC9mAH3jlcG7Aa1-A7Y023xqaNR8-g31_fOtMAVnFQm8ooG0cBGtN-Xd1DW7WyAfa_9110f3F-d3qVXN9czk9n10nFpKQJMKFFWkuecUHSjHKeKwqFSBlo4FSkRVZVpNA5CE2kkpDHnzKomMqjqoHtouMhd-ndYw-hKxsTKrBWteD6UKaUZ3nGpZCRHv2hC9f7Nr4uqoIxkhaiiOpkUJV3IXioy6U3jfLrkpLys5MydlJ-dRLt4Xdi_9CA_pU_JUQwHcCTsbD-P6m8PZsNkR_5wpeM</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Okano, Denise S.</creator><creator>Penitente‐Filho, Jurandy M.</creator><creator>Gomez León, Victor E.</creator><creator>Maitan, Paula P.</creator><creator>Silveira, Camila O.</creator><creator>Waddington, Bruna</creator><creator>Díaz‐Miranda, Edgar A.</creator><creator>da Costa, Eduardo P.</creator><creator>Guimarães, Simone E. F.</creator><creator>Guimarães, José D.</creator><general>Blackwell Publishing Ltd</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-8592-4020</orcidid><orcidid>https://orcid.org/0000-0001-6677-9609</orcidid></search><sort><creationdate>201903</creationdate><title>In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination</title><author>Okano, Denise S. ; Penitente‐Filho, Jurandy M. ; Gomez León, Victor E. ; Maitan, Paula P. ; Silveira, Camila O. ; Waddington, Bruna ; Díaz‐Miranda, Edgar A. ; da Costa, Eduardo P. ; Guimarães, Simone E. F. ; Guimarães, José D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3881-e37d72f864670241665a1e9723ede617294cc09d5e7d08a8e50153ec3a5e97fe3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Artificial insemination</topic><topic>Capacitation</topic><topic>Cattle</topic><topic>Cryopreservation</topic><topic>Fertility</topic><topic>flow cytometer</topic><topic>Hydrogen peroxide</topic><topic>Injury analysis</topic><topic>Integrity</topic><topic>Membranes</topic><topic>Motility</topic><topic>Multivariate analysis</topic><topic>Pregnancy</topic><topic>Regression analysis</topic><topic>Reproduction (biology)</topic><topic>Semen</topic><topic>semen fertility</topic><topic>Sperm</topic><topic>Statistical analysis</topic><topic>Thawing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Okano, Denise S.</creatorcontrib><creatorcontrib>Penitente‐Filho, Jurandy M.</creatorcontrib><creatorcontrib>Gomez León, Victor E.</creatorcontrib><creatorcontrib>Maitan, Paula P.</creatorcontrib><creatorcontrib>Silveira, Camila O.</creatorcontrib><creatorcontrib>Waddington, Bruna</creatorcontrib><creatorcontrib>Díaz‐Miranda, Edgar A.</creatorcontrib><creatorcontrib>da Costa, Eduardo P.</creatorcontrib><creatorcontrib>Guimarães, Simone E. F.</creatorcontrib><creatorcontrib>Guimarães, José D.</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Okano, Denise S.</au><au>Penitente‐Filho, Jurandy M.</au><au>Gomez León, Victor E.</au><au>Maitan, Paula P.</au><au>Silveira, Camila O.</au><au>Waddington, Bruna</au><au>Díaz‐Miranda, Edgar A.</au><au>da Costa, Eduardo P.</au><au>Guimarães, Simone E. F.</au><au>Guimarães, José D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2019-03</date><risdate>2019</risdate><volume>54</volume><issue>3</issue><spage>604</spage><epage>612</epage><pages>604-612</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
This study aimed to assess characteristics of bovine cryopreserved sperm and evaluate its relation to field fertility in fixed‐time artificial insemination (FTAI). Semen samples of 16 bulls were used to inseminate 811 Nellore cows, and four of these bulls were also used to inseminate 101 Nellore heifers. Samples of the same ejaculate used for FTAI from each bull were analysed in the laboratory after thawing. Sperm motility and vigour were subjectively assessed by light microscope, and integrity of the plasma and acrosome membranes, and H2O2 production were evaluated by flow cytometer. Relation among sperm characteristics and pregnancy rate of cows and heifers were evaluated by univariate and multivariate logistic regression. Subjective sperm motility and vigour did not affect the probability of pregnancy in cows or heifers. In univariate analysis for pregnancy in cows, sperm traits related to acrosome injury positively affected probability of pregnancy mainly when associated with plasma membrane integrity; H2O2 production seems to be less important than plasma membrane integrity in affecting probability of pregnancy. In multivariate analysis, sperm traits related to injured acrosome positively affected probability of cow and heifer pregnancies while intact acrosome was negatively related to cow pregnancy. Intact plasma membrane and high H2O2 production were positively related to cow pregnancy but negatively related to heifer pregnancy. Results suggest that a capacitation‐like status of the acrosome may benefit probability of pregnancy in cows.</abstract><cop>Germany</cop><pub>Blackwell Publishing Ltd</pub><pmid>30614080</pmid><doi>10.1111/rda.13401</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0002-8592-4020</orcidid><orcidid>https://orcid.org/0000-0001-6677-9609</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Reproduction in domestic animals, 2019-03, Vol.54 (3), p.604-612 |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Artificial insemination Capacitation Cattle Cryopreservation Fertility flow cytometer Hydrogen peroxide Injury analysis Integrity Membranes Motility Multivariate analysis Pregnancy Regression analysis Reproduction (biology) Semen semen fertility Sperm Statistical analysis Thawing |
| title | In vitro evaluation of cryopreserved bovine sperm and its relation to field fertility in fixed‐time artificial insemination |
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