Simple protocol for sequence-specific detection of mixed-base nucleic acids using a smart probe with NABs

A fluorescent smart probe (SP) was used to detect a mixed-base ribonucleic acids sequence. While the SP presents excellent sensitivity for the target, it gives subtle discrimination between the perfect target sequence and several mismatch sequences. Its sequence-specificity for the target was greatl...

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Veröffentlicht in:	Analytical biochemistry 2019-03, Vol.568, p.53-56
Hauptverfasser:	Oladepo, Sulayman A., Yusuf, Basiru O.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biosensing Techniques Fluorescent Dyes - chemistry MicroRNAs - analysis Nucleic Acid Amplification Techniques Oligonucleotide Probes - chemistry
Online-Zugang:	Volltext
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