Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil
High rates of paracoccidioidomycosis disease and infection have been recently reported in southern Brazil. However, the knowledgment about the ecoepidemiology of Paracoccidioides spp. in the region is limited, therefore, this study aimed to evaluate the presence of Paracoccidioides spp. DNA in soil...
Gespeichert in:
| Veröffentlicht in: | Current microbiology 2019-02, Vol.76 (2), p.258-262 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 262 |
|---|---|
| container_issue | 2 |
| container_start_page | 258 |
| container_title | Current microbiology |
| container_volume | 76 |
| creator | Mendes, Josiara Furtado Von Groll, Andrea Poester, Vanice Rodrigues Brasil, Carolina Litchina Brandolt, Tchana Martinez Klafke, Gabriel Baracy Nogueira, Carlos Eduardo Wayne Meireles, Mário Carlos Araújo Xavier, Melissa Orzechowski |
| description | High rates of paracoccidioidomycosis disease and infection have been recently reported in southern Brazil. However, the knowledgment about the ecoepidemiology of
Paracoccidioides
spp. in the region is limited, therefore, this study aimed to evaluate the presence of
Paracoccidioides
spp. DNA in soil samples from a rural area in Southern Rio Grande do Sul, Brazil. Thirty pools of soil samples from Bagé, RS (31º19′53"S 54º06′25"W) were submitted to physicochemical analysis, and to fungal DNA extraction by Norgen Biotek® Kit (Thorold, Canada), followed by Nested PCR technique with ITS4 and ITS5 as external primers, and PBITS-E and PBITS-T as internal primers. DNA amplification products of about 424 bp compatible with
Paracoccidioides
spp. were detected in eight (26.7%) of the 30 pools of samples, being three were sequenced and identified as
P. brasiliensis
. Positive soils were characterized by high levels of humidity, organic matter, basic saturation, and pH. This study shows for the first time the presence of
Paracoccidioides
spp. DNA at soils from the Brazilian Pampa Biome, proving that people living in those areas are exposed to the main agent of paracoccidioidomycosis. |
| doi_str_mv | 10.1007/s00284-018-1621-y |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2162773798</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2162262398</sourcerecordid><originalsourceid>FETCH-LOGICAL-c372t-3f360f8dfb4acc20a72fdad34038d34c8fcea77f203221f6f89260842de1476b3</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMoun78AC9S8OIlOklqkh518QsWFNRzyKaJRtqmJtvD-uuN21VB8JKBzDPvDA9ChwROCYA4SwBUlhiIxIRTgpcbaEJKRjFUFdlEE2Alw5Kfkx20m9IbAKEVkG20w4ADMM4m6OpBR22CMb72wdc2FanvTwvfFY_BN4WLoS0Wr7Z40G2vi0sfWjs2h_wbu-Iy6g_f7KMtp5tkD9Z1Dz1fXz1Nb_Hs_uZuejHDhgm6wMwxDk7Wbl5qYyhoQV2ta1YCk_k10hmrhXAUGKXEcScrykGWtLakFHzO9tDJmNvH8D7YtFCtT8Y2je5sGJKiWYMQTFQyo8d_0LcwxC5ft6Iop2xFkZEyMaQUrVN99K2OS0VAfTlWo2OVHasvx2qZZ47WycO8tfXPxLfUDNARSLnVvdj4u_r_1E_oQIVN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2162262398</pqid></control><display><type>article</type><title>Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil</title><source>SpringerLink Journals - AutoHoldings</source><creator>Mendes, Josiara Furtado ; Von Groll, Andrea ; Poester, Vanice Rodrigues ; Brasil, Carolina Litchina ; Brandolt, Tchana Martinez ; Klafke, Gabriel Baracy ; Nogueira, Carlos Eduardo Wayne ; Meireles, Mário Carlos Araújo ; Xavier, Melissa Orzechowski</creator><creatorcontrib>Mendes, Josiara Furtado ; Von Groll, Andrea ; Poester, Vanice Rodrigues ; Brasil, Carolina Litchina ; Brandolt, Tchana Martinez ; Klafke, Gabriel Baracy ; Nogueira, Carlos Eduardo Wayne ; Meireles, Mário Carlos Araújo ; Xavier, Melissa Orzechowski</creatorcontrib><description>High rates of paracoccidioidomycosis disease and infection have been recently reported in southern Brazil. However, the knowledgment about the ecoepidemiology of
Paracoccidioides
spp. in the region is limited, therefore, this study aimed to evaluate the presence of
Paracoccidioides
spp. DNA in soil samples from a rural area in Southern Rio Grande do Sul, Brazil. Thirty pools of soil samples from Bagé, RS (31º19′53"S 54º06′25"W) were submitted to physicochemical analysis, and to fungal DNA extraction by Norgen Biotek® Kit (Thorold, Canada), followed by Nested PCR technique with ITS4 and ITS5 as external primers, and PBITS-E and PBITS-T as internal primers. DNA amplification products of about 424 bp compatible with
Paracoccidioides
spp. were detected in eight (26.7%) of the 30 pools of samples, being three were sequenced and identified as
P. brasiliensis
. Positive soils were characterized by high levels of humidity, organic matter, basic saturation, and pH. This study shows for the first time the presence of
Paracoccidioides
spp. DNA at soils from the Brazilian Pampa Biome, proving that people living in those areas are exposed to the main agent of paracoccidioidomycosis.</description><identifier>ISSN: 0343-8651</identifier><identifier>EISSN: 1432-0991</identifier><identifier>DOI: 10.1007/s00284-018-1621-y</identifier><identifier>PMID: 30600363</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Biomedical and Life Sciences ; Biotechnology ; Deoxyribonucleic acid ; DNA ; Ecosystems ; Life Sciences ; Microbiology ; Organic matter ; Paracoccidioides ; Physicochemical analysis ; Primers ; Rural areas ; Soils ; South American blastomycosis</subject><ispartof>Current microbiology, 2019-02, Vol.76 (2), p.258-262</ispartof><rights>Springer Science+Business Media, LLC, part of Springer Nature 2019</rights><rights>Current Microbiology is a copyright of Springer, (2019). All Rights Reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c372t-3f360f8dfb4acc20a72fdad34038d34c8fcea77f203221f6f89260842de1476b3</citedby><cites>FETCH-LOGICAL-c372t-3f360f8dfb4acc20a72fdad34038d34c8fcea77f203221f6f89260842de1476b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00284-018-1621-y$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00284-018-1621-y$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30600363$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mendes, Josiara Furtado</creatorcontrib><creatorcontrib>Von Groll, Andrea</creatorcontrib><creatorcontrib>Poester, Vanice Rodrigues</creatorcontrib><creatorcontrib>Brasil, Carolina Litchina</creatorcontrib><creatorcontrib>Brandolt, Tchana Martinez</creatorcontrib><creatorcontrib>Klafke, Gabriel Baracy</creatorcontrib><creatorcontrib>Nogueira, Carlos Eduardo Wayne</creatorcontrib><creatorcontrib>Meireles, Mário Carlos Araújo</creatorcontrib><creatorcontrib>Xavier, Melissa Orzechowski</creatorcontrib><title>Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil</title><title>Current microbiology</title><addtitle>Curr Microbiol</addtitle><addtitle>Curr Microbiol</addtitle><description>High rates of paracoccidioidomycosis disease and infection have been recently reported in southern Brazil. However, the knowledgment about the ecoepidemiology of
Paracoccidioides
spp. in the region is limited, therefore, this study aimed to evaluate the presence of
Paracoccidioides
spp. DNA in soil samples from a rural area in Southern Rio Grande do Sul, Brazil. Thirty pools of soil samples from Bagé, RS (31º19′53"S 54º06′25"W) were submitted to physicochemical analysis, and to fungal DNA extraction by Norgen Biotek® Kit (Thorold, Canada), followed by Nested PCR technique with ITS4 and ITS5 as external primers, and PBITS-E and PBITS-T as internal primers. DNA amplification products of about 424 bp compatible with
Paracoccidioides
spp. were detected in eight (26.7%) of the 30 pools of samples, being three were sequenced and identified as
P. brasiliensis
. Positive soils were characterized by high levels of humidity, organic matter, basic saturation, and pH. This study shows for the first time the presence of
Paracoccidioides
spp. DNA at soils from the Brazilian Pampa Biome, proving that people living in those areas are exposed to the main agent of paracoccidioidomycosis.</description><subject>Biomedical and Life Sciences</subject><subject>Biotechnology</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Ecosystems</subject><subject>Life Sciences</subject><subject>Microbiology</subject><subject>Organic matter</subject><subject>Paracoccidioides</subject><subject>Physicochemical analysis</subject><subject>Primers</subject><subject>Rural areas</subject><subject>Soils</subject><subject>South American blastomycosis</subject><issn>0343-8651</issn><issn>1432-0991</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNp1kE1LxDAQhoMoun78AC9S8OIlOklqkh518QsWFNRzyKaJRtqmJtvD-uuN21VB8JKBzDPvDA9ChwROCYA4SwBUlhiIxIRTgpcbaEJKRjFUFdlEE2Alw5Kfkx20m9IbAKEVkG20w4ADMM4m6OpBR22CMb72wdc2FanvTwvfFY_BN4WLoS0Wr7Z40G2vi0sfWjs2h_wbu-Iy6g_f7KMtp5tkD9Z1Dz1fXz1Nb_Hs_uZuejHDhgm6wMwxDk7Wbl5qYyhoQV2ta1YCk_k10hmrhXAUGKXEcScrykGWtLakFHzO9tDJmNvH8D7YtFCtT8Y2je5sGJKiWYMQTFQyo8d_0LcwxC5ft6Iop2xFkZEyMaQUrVN99K2OS0VAfTlWo2OVHasvx2qZZ47WycO8tfXPxLfUDNARSLnVvdj4u_r_1E_oQIVN</recordid><startdate>20190201</startdate><enddate>20190201</enddate><creator>Mendes, Josiara Furtado</creator><creator>Von Groll, Andrea</creator><creator>Poester, Vanice Rodrigues</creator><creator>Brasil, Carolina Litchina</creator><creator>Brandolt, Tchana Martinez</creator><creator>Klafke, Gabriel Baracy</creator><creator>Nogueira, Carlos Eduardo Wayne</creator><creator>Meireles, Mário Carlos Araújo</creator><creator>Xavier, Melissa Orzechowski</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7N</scope><scope>M7P</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20190201</creationdate><title>Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil</title><author>Mendes, Josiara Furtado ; Von Groll, Andrea ; Poester, Vanice Rodrigues ; Brasil, Carolina Litchina ; Brandolt, Tchana Martinez ; Klafke, Gabriel Baracy ; Nogueira, Carlos Eduardo Wayne ; Meireles, Mário Carlos Araújo ; Xavier, Melissa Orzechowski</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c372t-3f360f8dfb4acc20a72fdad34038d34c8fcea77f203221f6f89260842de1476b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Biomedical and Life Sciences</topic><topic>Biotechnology</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Ecosystems</topic><topic>Life Sciences</topic><topic>Microbiology</topic><topic>Organic matter</topic><topic>Paracoccidioides</topic><topic>Physicochemical analysis</topic><topic>Primers</topic><topic>Rural areas</topic><topic>Soils</topic><topic>South American blastomycosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mendes, Josiara Furtado</creatorcontrib><creatorcontrib>Von Groll, Andrea</creatorcontrib><creatorcontrib>Poester, Vanice Rodrigues</creatorcontrib><creatorcontrib>Brasil, Carolina Litchina</creatorcontrib><creatorcontrib>Brandolt, Tchana Martinez</creatorcontrib><creatorcontrib>Klafke, Gabriel Baracy</creatorcontrib><creatorcontrib>Nogueira, Carlos Eduardo Wayne</creatorcontrib><creatorcontrib>Meireles, Mário Carlos Araújo</creatorcontrib><creatorcontrib>Xavier, Melissa Orzechowski</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mendes, Josiara Furtado</au><au>Von Groll, Andrea</au><au>Poester, Vanice Rodrigues</au><au>Brasil, Carolina Litchina</au><au>Brandolt, Tchana Martinez</au><au>Klafke, Gabriel Baracy</au><au>Nogueira, Carlos Eduardo Wayne</au><au>Meireles, Mário Carlos Araújo</au><au>Xavier, Melissa Orzechowski</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil</atitle><jtitle>Current microbiology</jtitle><stitle>Curr Microbiol</stitle><addtitle>Curr Microbiol</addtitle><date>2019-02-01</date><risdate>2019</risdate><volume>76</volume><issue>2</issue><spage>258</spage><epage>262</epage><pages>258-262</pages><issn>0343-8651</issn><eissn>1432-0991</eissn><abstract>High rates of paracoccidioidomycosis disease and infection have been recently reported in southern Brazil. However, the knowledgment about the ecoepidemiology of
Paracoccidioides
spp. in the region is limited, therefore, this study aimed to evaluate the presence of
Paracoccidioides
spp. DNA in soil samples from a rural area in Southern Rio Grande do Sul, Brazil. Thirty pools of soil samples from Bagé, RS (31º19′53"S 54º06′25"W) were submitted to physicochemical analysis, and to fungal DNA extraction by Norgen Biotek® Kit (Thorold, Canada), followed by Nested PCR technique with ITS4 and ITS5 as external primers, and PBITS-E and PBITS-T as internal primers. DNA amplification products of about 424 bp compatible with
Paracoccidioides
spp. were detected in eight (26.7%) of the 30 pools of samples, being three were sequenced and identified as
P. brasiliensis
. Positive soils were characterized by high levels of humidity, organic matter, basic saturation, and pH. This study shows for the first time the presence of
Paracoccidioides
spp. DNA at soils from the Brazilian Pampa Biome, proving that people living in those areas are exposed to the main agent of paracoccidioidomycosis.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>30600363</pmid><doi>10.1007/s00284-018-1621-y</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0343-8651 |
| ispartof | Current microbiology, 2019-02, Vol.76 (2), p.258-262 |
| issn | 0343-8651 1432-0991 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2162773798 |
| source | SpringerLink Journals - AutoHoldings |
| subjects | Biomedical and Life Sciences Biotechnology Deoxyribonucleic acid DNA Ecosystems Life Sciences Microbiology Organic matter Paracoccidioides Physicochemical analysis Primers Rural areas Soils South American blastomycosis |
| title | Paracoccidioides spp. in Soil from the Pampa Biome in Southern Brazil |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-06T11%3A58%3A50IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Paracoccidioides%20spp.%20in%20Soil%20from%20the%20Pampa%20Biome%20in%20Southern%20Brazil&rft.jtitle=Current%20microbiology&rft.au=Mendes,%20Josiara%20Furtado&rft.date=2019-02-01&rft.volume=76&rft.issue=2&rft.spage=258&rft.epage=262&rft.pages=258-262&rft.issn=0343-8651&rft.eissn=1432-0991&rft_id=info:doi/10.1007/s00284-018-1621-y&rft_dat=%3Cproquest_cross%3E2162262398%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2162262398&rft_id=info:pmid/30600363&rfr_iscdi=true |