The use of ROI overlays and a semi‐automated method for measuring cortical area in ImageJ for histological analysis
A major part of histologic studies is the use of high resolution imaging for data collection and analysis. ImageJ, a freely available software from the NIH designed for image analysis, has many features that are not well‐known among bone histologists and can be incredibly beneficial in terms of stre...
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| Veröffentlicht in: | American journal of physical anthropology 2019-02, Vol.168 (2), p.378-382 |
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| container_title | American journal of physical anthropology |
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| creator | Dominguez, Victoria M. Agnew, Amanda M. |
| description | A major part of histologic studies is the use of high resolution imaging for data collection and analysis. ImageJ, a freely available software from the NIH designed for image analysis, has many features that are not well‐known among bone histologists and can be incredibly beneficial in terms of stream‐lining data collection and maximizing limited resources. The aims of this technical note are twofold: (a) to describe methods for image annotation and measurement using region of interest overlays in ImageJ, and (b) to present a new code for a semi‐automated method of measuring cortical bone areas from high resolution cross‐sectional images also using ImageJ. |
| doi_str_mv | 10.1002/ajpa.23747 |
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| ispartof | American journal of physical anthropology, 2019-02, Vol.168 (2), p.378-382 |
| issn | 0002-9483 1096-8644 2692-7691 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Anthropology, Physical Automation bone histology Bones cortical area Cortical Bone - diagnostic imaging Data collection Databases, Factual Histological Techniques - methods histomorphometry Image Processing, Computer-Assisted - methods ImageJ Measurement Models, Biological Software |
| title | The use of ROI overlays and a semi‐automated method for measuring cortical area in ImageJ for histological analysis |
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