Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes
•DNA damage is more in lifeguards than in the control group.•DNA damage was significantly higher in female lifeguards in comparison with males.•Long term exposure to Chlorine in indoor pools increases the DNA damage. Chlorination has been used as a major disinfectant process for swimming pool water...
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| Veröffentlicht in: | Mutation research. Genetic toxicology and environmental mutagenesis 2019-01, Vol.837, p.29-33 |
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| container_title | Mutation research. Genetic toxicology and environmental mutagenesis |
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| creator | Kianmehr, Mojtaba Mottaghy Shahri, Mahmood Reza Afsharnia, Mojtaba Rohani, Zahra Ghorbani, Mohammad |
| description | •DNA damage is more in lifeguards than in the control group.•DNA damage was significantly higher in female lifeguards in comparison with males.•Long term exposure to Chlorine in indoor pools increases the DNA damage.
Chlorination has been used as a major disinfectant process for swimming pool water in many countries. The purpose of this study is to compare the DNA damage of the blood lymphocytes in indoor pool lifeguards with non-lifeguards athletes. We performed a study in which the participants were Gonabad’s lifeguards. We chose 30 participants (15 male and 15 female) for each group. We collected vein blood samples from each participant in both exposed and control group. The lymphocytes were isolated from the whole blood by ficoll, and the cell viability was determined by the trypan blue. The alkaline Comet assay was also performed on lymphocytes in order to measure the DNA damage. All the parameters indicated that the DNA damage was significantly greater in lifeguards group than control group (p |
| doi_str_mv | 10.1016/j.mrgentox.2018.09.005 |
| format | Article |
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Chlorination has been used as a major disinfectant process for swimming pool water in many countries. The purpose of this study is to compare the DNA damage of the blood lymphocytes in indoor pool lifeguards with non-lifeguards athletes. We performed a study in which the participants were Gonabad’s lifeguards. We chose 30 participants (15 male and 15 female) for each group. We collected vein blood samples from each participant in both exposed and control group. The lymphocytes were isolated from the whole blood by ficoll, and the cell viability was determined by the trypan blue. The alkaline Comet assay was also performed on lymphocytes in order to measure the DNA damage. All the parameters indicated that the DNA damage was significantly greater in lifeguards group than control group (p < 0.001). Also, the results revealed a statistically significant higher level of DNA damage in females as evident by an increase in the tail length (μm) [8.97 ± 4.21 for females as compared to 4.32 ± 1.33 for males (p = 0.001)], tail DNA (%) [4.18 ± 1.27 for females as compared to 3.14 ± 0.94 for males (p = 0.016)] and tail moment (μm) [0.68 ± 0.53 for females and 0.26 ± 0.14 for males (p = 0.010)]. There was also a significant positive correlation between DNA damage and the duration of work (P < 0.001).</description><identifier>ISSN: 1383-5718</identifier><identifier>EISSN: 1879-3592</identifier><identifier>DOI: 10.1016/j.mrgentox.2018.09.005</identifier><identifier>PMID: 30595206</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Chlorine ; DNA damage ; Indoor swimming pool ; Lifeguards</subject><ispartof>Mutation research. Genetic toxicology and environmental mutagenesis, 2019-01, Vol.837, p.29-33</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-f5742de3254fbfb759b84a65525b01ba4df1c3f39eb3ebc897934d37dd9ba45a3</citedby><cites>FETCH-LOGICAL-c368t-f5742de3254fbfb759b84a65525b01ba4df1c3f39eb3ebc897934d37dd9ba45a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.mrgentox.2018.09.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30595206$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kianmehr, Mojtaba</creatorcontrib><creatorcontrib>Mottaghy Shahri, Mahmood Reza</creatorcontrib><creatorcontrib>Afsharnia, Mojtaba</creatorcontrib><creatorcontrib>Rohani, Zahra</creatorcontrib><creatorcontrib>Ghorbani, Mohammad</creatorcontrib><title>Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes</title><title>Mutation research. Genetic toxicology and environmental mutagenesis</title><addtitle>Mutat Res Genet Toxicol Environ Mutagen</addtitle><description>•DNA damage is more in lifeguards than in the control group.•DNA damage was significantly higher in female lifeguards in comparison with males.•Long term exposure to Chlorine in indoor pools increases the DNA damage.
Chlorination has been used as a major disinfectant process for swimming pool water in many countries. The purpose of this study is to compare the DNA damage of the blood lymphocytes in indoor pool lifeguards with non-lifeguards athletes. We performed a study in which the participants were Gonabad’s lifeguards. We chose 30 participants (15 male and 15 female) for each group. We collected vein blood samples from each participant in both exposed and control group. The lymphocytes were isolated from the whole blood by ficoll, and the cell viability was determined by the trypan blue. The alkaline Comet assay was also performed on lymphocytes in order to measure the DNA damage. All the parameters indicated that the DNA damage was significantly greater in lifeguards group than control group (p < 0.001). Also, the results revealed a statistically significant higher level of DNA damage in females as evident by an increase in the tail length (μm) [8.97 ± 4.21 for females as compared to 4.32 ± 1.33 for males (p = 0.001)], tail DNA (%) [4.18 ± 1.27 for females as compared to 3.14 ± 0.94 for males (p = 0.016)] and tail moment (μm) [0.68 ± 0.53 for females and 0.26 ± 0.14 for males (p = 0.010)]. There was also a significant positive correlation between DNA damage and the duration of work (P < 0.001).</description><subject>Chlorine</subject><subject>DNA damage</subject><subject>Indoor swimming pool</subject><subject>Lifeguards</subject><issn>1383-5718</issn><issn>1879-3592</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNqFkE1v1DAQhq0KREvhL1Q-cknwR5zEt1ZL-ZCq9gJnyx-TXa9iO9hZyv57XG2LuHHyaOZ5PZoHoStKWkpo_3HfhryFuKbfLSN0bIlsCRFn6IKOg2y4kOxVrfnIGzHQ8Ry9LWVPCCOcjG_QOSdCCkb6CxQ3KSw6-5IiThP-dH-DnQ56CwX7iM2cksPzMSy7ZI9rbVbGR5dSxuXRh-DjFi8pzXj2E2wPOruCH_26wzHF5p-eXncz1Pw79HrSc4H3z-8l-vH59vvma3P38OXb5uausbwf12YSQ8cccCa6yUxmENKMne6FYMIQanTnJmr5xCUYDsaOcpC8c3xwTtah0PwSfTj9u-T08wBlVcEXC_OsI6RDUYz2VDJBCatof0JtTqVkmNSSfdD5qChRT67VXr24Vk-uFZGquq7Bq-cdBxPA_Y29yK3A9QmAeukvD1kV6yFacD6DXZVL_n87_gCUbJY9</recordid><startdate>201901</startdate><enddate>201901</enddate><creator>Kianmehr, Mojtaba</creator><creator>Mottaghy Shahri, Mahmood Reza</creator><creator>Afsharnia, Mojtaba</creator><creator>Rohani, Zahra</creator><creator>Ghorbani, Mohammad</creator><general>Elsevier B.V</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201901</creationdate><title>Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes</title><author>Kianmehr, Mojtaba ; Mottaghy Shahri, Mahmood Reza ; Afsharnia, Mojtaba ; Rohani, Zahra ; Ghorbani, Mohammad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-f5742de3254fbfb759b84a65525b01ba4df1c3f39eb3ebc897934d37dd9ba45a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Chlorine</topic><topic>DNA damage</topic><topic>Indoor swimming pool</topic><topic>Lifeguards</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kianmehr, Mojtaba</creatorcontrib><creatorcontrib>Mottaghy Shahri, Mahmood Reza</creatorcontrib><creatorcontrib>Afsharnia, Mojtaba</creatorcontrib><creatorcontrib>Rohani, Zahra</creatorcontrib><creatorcontrib>Ghorbani, Mohammad</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Mutation research. Genetic toxicology and environmental mutagenesis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kianmehr, Mojtaba</au><au>Mottaghy Shahri, Mahmood Reza</au><au>Afsharnia, Mojtaba</au><au>Rohani, Zahra</au><au>Ghorbani, Mohammad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes</atitle><jtitle>Mutation research. Genetic toxicology and environmental mutagenesis</jtitle><addtitle>Mutat Res Genet Toxicol Environ Mutagen</addtitle><date>2019-01</date><risdate>2019</risdate><volume>837</volume><spage>29</spage><epage>33</epage><pages>29-33</pages><issn>1383-5718</issn><eissn>1879-3592</eissn><abstract>•DNA damage is more in lifeguards than in the control group.•DNA damage was significantly higher in female lifeguards in comparison with males.•Long term exposure to Chlorine in indoor pools increases the DNA damage.
Chlorination has been used as a major disinfectant process for swimming pool water in many countries. The purpose of this study is to compare the DNA damage of the blood lymphocytes in indoor pool lifeguards with non-lifeguards athletes. We performed a study in which the participants were Gonabad’s lifeguards. We chose 30 participants (15 male and 15 female) for each group. We collected vein blood samples from each participant in both exposed and control group. The lymphocytes were isolated from the whole blood by ficoll, and the cell viability was determined by the trypan blue. The alkaline Comet assay was also performed on lymphocytes in order to measure the DNA damage. All the parameters indicated that the DNA damage was significantly greater in lifeguards group than control group (p < 0.001). Also, the results revealed a statistically significant higher level of DNA damage in females as evident by an increase in the tail length (μm) [8.97 ± 4.21 for females as compared to 4.32 ± 1.33 for males (p = 0.001)], tail DNA (%) [4.18 ± 1.27 for females as compared to 3.14 ± 0.94 for males (p = 0.016)] and tail moment (μm) [0.68 ± 0.53 for females and 0.26 ± 0.14 for males (p = 0.010)]. There was also a significant positive correlation between DNA damage and the duration of work (P < 0.001).</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30595206</pmid><doi>10.1016/j.mrgentox.2018.09.005</doi><tpages>5</tpages></addata></record> |
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| ispartof | Mutation research. Genetic toxicology and environmental mutagenesis, 2019-01, Vol.837, p.29-33 |
| issn | 1383-5718 1879-3592 |
| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Chlorine DNA damage Indoor swimming pool Lifeguards |
| title | Comparison of DNA damages in blood lymphocytes of indoor swimming pool lifeguards with non-lifeguards athletes |
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