Supplementation of Glial Cell Line-Derived Neurotrophic Factor, Fibroblast Growth Factor 2, and Epidermal Growth Factor Promotes Self-Renewal of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells by Upregulating the Expression of miR-20b, miR-21, and miR-106a
In this study, we investigated the effect of glial cell line-derived neurotrophic factor (GDNF), fibroblast growth factor (FGF) 2, and epidermal growth factor (EGF) on the expression of some self-renewal-related microRNAs (miRs) in putative buffalo spermatogonial stem cells (SSCs). The SSCs were cul...
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| Veröffentlicht in: | Cellular reprogramming 2019-02, Vol.21 (1), p.11-17 |
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| container_title | Cellular reprogramming |
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| creator | Sharma, Ankur Lagah, Swati Viviyan Nagoorvali, Dudekula Kumar, B S Bharath Singh, Manoj Kumar Singla, Suresh Kumar Manik, Radhay Sham Palta, Prabhat Chauhan, Manmohan Singh |
| description | In this study, we investigated the effect of glial cell line-derived neurotrophic factor (GDNF), fibroblast growth factor (FGF) 2, and epidermal growth factor (EGF) on the expression of some self-renewal-related microRNAs (miRs) in putative buffalo spermatogonial stem cells (SSCs). The SSCs were cultured on a buffalo Sertoli cell feeder layer, colony formation was observed between 7 and 10 days. The SSC colonies expressed markers specific for undifferentiated type A spermatogonia and pluripotency markers. After 15 days of initial culture, the colonies were subcultured as treatment (supplemented with 20 ng mL
GDNF +10 ng mL
FGF2 + 10 ng mL
EGF) and control groups. The number and area of SSC colonies were significantly (p |
| doi_str_mv | 10.1089/cell.2018.0034 |
| format | Article |
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GDNF +10 ng mL
FGF2 + 10 ng mL
EGF) and control groups. The number and area of SSC colonies were significantly (p < 0.05) higher in the treatment group than in the control group. The relative abundance of miR-20b, miR-21, and miR-106a in SSCs supplemented with growth factors was significantly higher (p < 0.001) than that in the control. The results indicate that supplementation of SSC culture medium with growth factors (GDNF, FGF2, and EGF) may promote the expression of miR-20b, miR-21, and miR-106a, which is essential for self-renewal and maintenance of SSCs.</description><identifier>ISSN: 2152-4971</identifier><identifier>EISSN: 2152-4998</identifier><identifier>DOI: 10.1089/cell.2018.0034</identifier><identifier>PMID: 30589561</identifier><language>eng</language><publisher>United States</publisher><ispartof>Cellular reprogramming, 2019-02, Vol.21 (1), p.11-17</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c295t-4dc0c68ef87f7db4c1a4b9bda2252254da6347c06bbf27c13c708d3a3d0e2e793</citedby><cites>FETCH-LOGICAL-c295t-4dc0c68ef87f7db4c1a4b9bda2252254da6347c06bbf27c13c708d3a3d0e2e793</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30589561$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sharma, Ankur</creatorcontrib><creatorcontrib>Lagah, Swati Viviyan</creatorcontrib><creatorcontrib>Nagoorvali, Dudekula</creatorcontrib><creatorcontrib>Kumar, B S Bharath</creatorcontrib><creatorcontrib>Singh, Manoj Kumar</creatorcontrib><creatorcontrib>Singla, Suresh Kumar</creatorcontrib><creatorcontrib>Manik, Radhay Sham</creatorcontrib><creatorcontrib>Palta, Prabhat</creatorcontrib><creatorcontrib>Chauhan, Manmohan Singh</creatorcontrib><title>Supplementation of Glial Cell Line-Derived Neurotrophic Factor, Fibroblast Growth Factor 2, and Epidermal Growth Factor Promotes Self-Renewal of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells by Upregulating the Expression of miR-20b, miR-21, and miR-106a</title><title>Cellular reprogramming</title><addtitle>Cell Reprogram</addtitle><description>In this study, we investigated the effect of glial cell line-derived neurotrophic factor (GDNF), fibroblast growth factor (FGF) 2, and epidermal growth factor (EGF) on the expression of some self-renewal-related microRNAs (miRs) in putative buffalo spermatogonial stem cells (SSCs). The SSCs were cultured on a buffalo Sertoli cell feeder layer, colony formation was observed between 7 and 10 days. The SSC colonies expressed markers specific for undifferentiated type A spermatogonia and pluripotency markers. After 15 days of initial culture, the colonies were subcultured as treatment (supplemented with 20 ng mL
GDNF +10 ng mL
FGF2 + 10 ng mL
EGF) and control groups. The number and area of SSC colonies were significantly (p < 0.05) higher in the treatment group than in the control group. The relative abundance of miR-20b, miR-21, and miR-106a in SSCs supplemented with growth factors was significantly higher (p < 0.001) than that in the control. The results indicate that supplementation of SSC culture medium with growth factors (GDNF, FGF2, and EGF) may promote the expression of miR-20b, miR-21, and miR-106a, which is essential for self-renewal and maintenance of SSCs.</description><issn>2152-4971</issn><issn>2152-4998</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNpVUk1vEzEQ3SIqWpVcOSIfi5RNbe_3kYYkIEWlauh5ZXtnEyPvevFHS_89NgmVsCzNyPPem2fNJMkHghcE182NAKUWFJN6gXGWv0kuKSlomjdN_fY1r8hFMrP2Jw4nywKtfJdcZLiom6Ikl2eznZ8mBQOMjjmpR6R7tFGSKbQM4mgrR0i_gJFP0KE78EY7o6eDFGjNhNNmjtaSG80Vsw5tjH52h1MF0TliY4dWk-zADEHw__K90YN2YNEOVJ8-wAjPARO63_vo5AnQre97pjS6vvWcKW8Rj1HaT2g3RUWn93qMTncOhr92A-QFPU4G9l4FjXGP3AHQ6nd4sfb0uUE-pBTz-TEhR5MxJ7hk75Pz0NLC7BSvksf16sfya7r9vvm2_LxNBW0Kl-adwKKsoa-rvup4LgjLecM7RmkRbt6xMssrgUvOe1oJkokK113Gsg4DharJrpLro-5k9C8P1rWDtHGabATtbUtJSSpMMS4CdHGECqOtNdC3k5EDMy8twW3cgjby2rgFbdyCQPh40vZ8gO4V_m_m2R-d8bA5</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Sharma, Ankur</creator><creator>Lagah, Swati Viviyan</creator><creator>Nagoorvali, Dudekula</creator><creator>Kumar, B S Bharath</creator><creator>Singh, Manoj Kumar</creator><creator>Singla, Suresh Kumar</creator><creator>Manik, Radhay Sham</creator><creator>Palta, Prabhat</creator><creator>Chauhan, Manmohan Singh</creator><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>Supplementation of Glial Cell Line-Derived Neurotrophic Factor, Fibroblast Growth Factor 2, and Epidermal Growth Factor Promotes Self-Renewal of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells by Upregulating the Expression of miR-20b, miR-21, and miR-106a</title><author>Sharma, Ankur ; Lagah, Swati Viviyan ; Nagoorvali, Dudekula ; Kumar, B S Bharath ; Singh, Manoj Kumar ; Singla, Suresh Kumar ; Manik, Radhay Sham ; Palta, Prabhat ; Chauhan, Manmohan Singh</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c295t-4dc0c68ef87f7db4c1a4b9bda2252254da6347c06bbf27c13c708d3a3d0e2e793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sharma, Ankur</creatorcontrib><creatorcontrib>Lagah, Swati Viviyan</creatorcontrib><creatorcontrib>Nagoorvali, Dudekula</creatorcontrib><creatorcontrib>Kumar, B S Bharath</creatorcontrib><creatorcontrib>Singh, Manoj Kumar</creatorcontrib><creatorcontrib>Singla, Suresh Kumar</creatorcontrib><creatorcontrib>Manik, Radhay Sham</creatorcontrib><creatorcontrib>Palta, Prabhat</creatorcontrib><creatorcontrib>Chauhan, Manmohan Singh</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cellular reprogramming</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sharma, Ankur</au><au>Lagah, Swati Viviyan</au><au>Nagoorvali, Dudekula</au><au>Kumar, B S Bharath</au><au>Singh, Manoj Kumar</au><au>Singla, Suresh Kumar</au><au>Manik, Radhay Sham</au><au>Palta, Prabhat</au><au>Chauhan, Manmohan Singh</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Supplementation of Glial Cell Line-Derived Neurotrophic Factor, Fibroblast Growth Factor 2, and Epidermal Growth Factor Promotes Self-Renewal of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells by Upregulating the Expression of miR-20b, miR-21, and miR-106a</atitle><jtitle>Cellular reprogramming</jtitle><addtitle>Cell Reprogram</addtitle><date>2019-02</date><risdate>2019</risdate><volume>21</volume><issue>1</issue><spage>11</spage><epage>17</epage><pages>11-17</pages><issn>2152-4971</issn><eissn>2152-4998</eissn><abstract>In this study, we investigated the effect of glial cell line-derived neurotrophic factor (GDNF), fibroblast growth factor (FGF) 2, and epidermal growth factor (EGF) on the expression of some self-renewal-related microRNAs (miRs) in putative buffalo spermatogonial stem cells (SSCs). The SSCs were cultured on a buffalo Sertoli cell feeder layer, colony formation was observed between 7 and 10 days. The SSC colonies expressed markers specific for undifferentiated type A spermatogonia and pluripotency markers. After 15 days of initial culture, the colonies were subcultured as treatment (supplemented with 20 ng mL
GDNF +10 ng mL
FGF2 + 10 ng mL
EGF) and control groups. The number and area of SSC colonies were significantly (p < 0.05) higher in the treatment group than in the control group. The relative abundance of miR-20b, miR-21, and miR-106a in SSCs supplemented with growth factors was significantly higher (p < 0.001) than that in the control. The results indicate that supplementation of SSC culture medium with growth factors (GDNF, FGF2, and EGF) may promote the expression of miR-20b, miR-21, and miR-106a, which is essential for self-renewal and maintenance of SSCs.</abstract><cop>United States</cop><pmid>30589561</pmid><doi>10.1089/cell.2018.0034</doi><tpages>7</tpages></addata></record> |
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| title | Supplementation of Glial Cell Line-Derived Neurotrophic Factor, Fibroblast Growth Factor 2, and Epidermal Growth Factor Promotes Self-Renewal of Putative Buffalo (Bubalus bubalis) Spermatogonial Stem Cells by Upregulating the Expression of miR-20b, miR-21, and miR-106a |
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