Identification and characterization of a novel nanobody against duck hepatitis A virus type 1
Duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1) is an acute and highly contagious disease affecting young ducklings. The VP1 protein is one of the major structural proteins of DHAV-1 carries critical epitopes responsible for the induction of neutralizing antibodies. In th...
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| Veröffentlicht in: | Virology (New York, N.Y.) N.Y.), 2019-02, Vol.528, p.101-109 |
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| creator | Xue, Wenxiang Zhao, Qin Li, Pengfei Zhang, Ruihua Lan, Jingjing Wang, Jingyu Yang, Xiaoxue Xie, Zhijing Jiang, Shijin |
| description | Duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1) is an acute and highly contagious disease affecting young ducklings. The VP1 protein is one of the major structural proteins of DHAV-1 carries critical epitopes responsible for the induction of neutralizing antibodies. In this study, we have successfully constructed an immune phage display VHHs library against DHAV-1 with the size of 6 × 106 colonies. A nanobody (Nb) against VP1 protein of DHAV-1, named Nb25, was identified from the immunized phage display library. Nb25 could react with the conserved linear B-cell epitope of 174PAPTST179 in DHAV-1 VP1, even though Nb25 showed no neutralizing activity to DHAV-1. To the best of our knowledge, this is the first report about preparation of anti-DHAV-1 Nbs and identification of the specific conserved linear B-cell epitope of DHAV-1 with Nb, which will facilitate the serologic diagnosis of DHAV-1 infection.
•A specific Nb against DHAV-1 VP1 protein is successfully produced.•Nb25 has good binding activity and specificity with DHAV-1 VP1.•A linear epitope of DHAV-1 was recognized by Nb25.•The epitope of 174PAPTST179 in VP1 was conserved among different DHAV-1 strains. |
| doi_str_mv | 10.1016/j.virol.2018.12.013 |
| format | Article |
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•A specific Nb against DHAV-1 VP1 protein is successfully produced.•Nb25 has good binding activity and specificity with DHAV-1 VP1.•A linear epitope of DHAV-1 was recognized by Nb25.•The epitope of 174PAPTST179 in VP1 was conserved among different DHAV-1 strains.</description><identifier>ISSN: 0042-6822</identifier><identifier>EISSN: 1096-0341</identifier><identifier>DOI: 10.1016/j.virol.2018.12.013</identifier><identifier>PMID: 30590261</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Cell Surface Display Techniques ; DHAV-1 ; Ducks ; Epitope ; Epitopes, B-Lymphocyte - immunology ; Hepatitis Virus, Duck - immunology ; Hepatitis, Viral, Animal - diagnosis ; Hepatitis, Viral, Animal - immunology ; Nanobody ; Phage display ; Poultry Diseases - virology ; Single-Domain Antibodies - isolation & purification ; Viral Structural Proteins - immunology ; VP1</subject><ispartof>Virology (New York, N.Y.), 2019-02, Vol.528, p.101-109</ispartof><rights>2018 Elsevier Inc.</rights><rights>Copyright © 2018 Elsevier Inc. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c359t-6e4f8da2597fff9d2cf73edf64aa00f97cec6bc8ec321ad8764f51bd9d719c523</citedby><cites>FETCH-LOGICAL-c359t-6e4f8da2597fff9d2cf73edf64aa00f97cec6bc8ec321ad8764f51bd9d719c523</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0042682218303763$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30590261$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Xue, Wenxiang</creatorcontrib><creatorcontrib>Zhao, Qin</creatorcontrib><creatorcontrib>Li, Pengfei</creatorcontrib><creatorcontrib>Zhang, Ruihua</creatorcontrib><creatorcontrib>Lan, Jingjing</creatorcontrib><creatorcontrib>Wang, Jingyu</creatorcontrib><creatorcontrib>Yang, Xiaoxue</creatorcontrib><creatorcontrib>Xie, Zhijing</creatorcontrib><creatorcontrib>Jiang, Shijin</creatorcontrib><title>Identification and characterization of a novel nanobody against duck hepatitis A virus type 1</title><title>Virology (New York, N.Y.)</title><addtitle>Virology</addtitle><description>Duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1) is an acute and highly contagious disease affecting young ducklings. The VP1 protein is one of the major structural proteins of DHAV-1 carries critical epitopes responsible for the induction of neutralizing antibodies. In this study, we have successfully constructed an immune phage display VHHs library against DHAV-1 with the size of 6 × 106 colonies. A nanobody (Nb) against VP1 protein of DHAV-1, named Nb25, was identified from the immunized phage display library. Nb25 could react with the conserved linear B-cell epitope of 174PAPTST179 in DHAV-1 VP1, even though Nb25 showed no neutralizing activity to DHAV-1. To the best of our knowledge, this is the first report about preparation of anti-DHAV-1 Nbs and identification of the specific conserved linear B-cell epitope of DHAV-1 with Nb, which will facilitate the serologic diagnosis of DHAV-1 infection.
•A specific Nb against DHAV-1 VP1 protein is successfully produced.•Nb25 has good binding activity and specificity with DHAV-1 VP1.•A linear epitope of DHAV-1 was recognized by Nb25.•The epitope of 174PAPTST179 in VP1 was conserved among different DHAV-1 strains.</description><subject>Animals</subject><subject>Cell Surface Display Techniques</subject><subject>DHAV-1</subject><subject>Ducks</subject><subject>Epitope</subject><subject>Epitopes, B-Lymphocyte - immunology</subject><subject>Hepatitis Virus, Duck - immunology</subject><subject>Hepatitis, Viral, Animal - diagnosis</subject><subject>Hepatitis, Viral, Animal - immunology</subject><subject>Nanobody</subject><subject>Phage display</subject><subject>Poultry Diseases - virology</subject><subject>Single-Domain Antibodies - isolation & purification</subject><subject>Viral Structural Proteins - immunology</subject><subject>VP1</subject><issn>0042-6822</issn><issn>1096-0341</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQQC0EokvhFyAhH7kkzDiJnRw4VFWBSpW4wBFZXntMvWTtxXZWWn49KVs4chrN6M3XY-w1QouA8t2uPYac5lYAji2KFrB7wjYIk2yg6_Ep2wD0opGjEBfsRSk7WHOl4Dm76GCYQEjcsG-3jmINPlhTQ4rcRMftvcnGVsrh17mYPDc8piPNPJqYtsmduPluQiyVu8X-4Pd0WMkaCr_i61FL4fV0II4v2TNv5kKvHuMl-_rh5sv1p-bu88fb66u7xnbDVBtJvR-dEcOkvPeTE9arjpyXvTEAflKWrNzakWwn0LhRyd4PuHWTUzjZQXSX7O157iGnnwuVqvehWJpnEyktRQuUqAB7BSvanVGbUymZvD7ksDf5pBH0g1e903-86gevGoVeva5dbx4XLNs9uX89f0WuwPszQOubx0BZFxsoWnIhk63apfDfBb8BjLaL_A</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Xue, Wenxiang</creator><creator>Zhao, Qin</creator><creator>Li, Pengfei</creator><creator>Zhang, Ruihua</creator><creator>Lan, Jingjing</creator><creator>Wang, Jingyu</creator><creator>Yang, Xiaoxue</creator><creator>Xie, Zhijing</creator><creator>Jiang, Shijin</creator><general>Elsevier Inc</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>Identification and characterization of a novel nanobody against duck hepatitis A virus type 1</title><author>Xue, Wenxiang ; Zhao, Qin ; Li, Pengfei ; Zhang, Ruihua ; Lan, Jingjing ; Wang, Jingyu ; Yang, Xiaoxue ; Xie, Zhijing ; Jiang, Shijin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c359t-6e4f8da2597fff9d2cf73edf64aa00f97cec6bc8ec321ad8764f51bd9d719c523</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Cell Surface Display Techniques</topic><topic>DHAV-1</topic><topic>Ducks</topic><topic>Epitope</topic><topic>Epitopes, B-Lymphocyte - immunology</topic><topic>Hepatitis Virus, Duck - immunology</topic><topic>Hepatitis, Viral, Animal - diagnosis</topic><topic>Hepatitis, Viral, Animal - immunology</topic><topic>Nanobody</topic><topic>Phage display</topic><topic>Poultry Diseases - virology</topic><topic>Single-Domain Antibodies - isolation & purification</topic><topic>Viral Structural Proteins - immunology</topic><topic>VP1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xue, Wenxiang</creatorcontrib><creatorcontrib>Zhao, Qin</creatorcontrib><creatorcontrib>Li, Pengfei</creatorcontrib><creatorcontrib>Zhang, Ruihua</creatorcontrib><creatorcontrib>Lan, Jingjing</creatorcontrib><creatorcontrib>Wang, Jingyu</creatorcontrib><creatorcontrib>Yang, Xiaoxue</creatorcontrib><creatorcontrib>Xie, Zhijing</creatorcontrib><creatorcontrib>Jiang, Shijin</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Virology (New York, N.Y.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Xue, Wenxiang</au><au>Zhao, Qin</au><au>Li, Pengfei</au><au>Zhang, Ruihua</au><au>Lan, Jingjing</au><au>Wang, Jingyu</au><au>Yang, Xiaoxue</au><au>Xie, Zhijing</au><au>Jiang, Shijin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of a novel nanobody against duck hepatitis A virus type 1</atitle><jtitle>Virology (New York, N.Y.)</jtitle><addtitle>Virology</addtitle><date>2019-02</date><risdate>2019</risdate><volume>528</volume><spage>101</spage><epage>109</epage><pages>101-109</pages><issn>0042-6822</issn><eissn>1096-0341</eissn><abstract>Duck virus hepatitis (DVH) caused by duck hepatitis A virus type 1 (DHAV-1) is an acute and highly contagious disease affecting young ducklings. The VP1 protein is one of the major structural proteins of DHAV-1 carries critical epitopes responsible for the induction of neutralizing antibodies. In this study, we have successfully constructed an immune phage display VHHs library against DHAV-1 with the size of 6 × 106 colonies. A nanobody (Nb) against VP1 protein of DHAV-1, named Nb25, was identified from the immunized phage display library. Nb25 could react with the conserved linear B-cell epitope of 174PAPTST179 in DHAV-1 VP1, even though Nb25 showed no neutralizing activity to DHAV-1. To the best of our knowledge, this is the first report about preparation of anti-DHAV-1 Nbs and identification of the specific conserved linear B-cell epitope of DHAV-1 with Nb, which will facilitate the serologic diagnosis of DHAV-1 infection.
•A specific Nb against DHAV-1 VP1 protein is successfully produced.•Nb25 has good binding activity and specificity with DHAV-1 VP1.•A linear epitope of DHAV-1 was recognized by Nb25.•The epitope of 174PAPTST179 in VP1 was conserved among different DHAV-1 strains.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>30590261</pmid><doi>10.1016/j.virol.2018.12.013</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Cell Surface Display Techniques DHAV-1 Ducks Epitope Epitopes, B-Lymphocyte - immunology Hepatitis Virus, Duck - immunology Hepatitis, Viral, Animal - diagnosis Hepatitis, Viral, Animal - immunology Nanobody Phage display Poultry Diseases - virology Single-Domain Antibodies - isolation & purification Viral Structural Proteins - immunology VP1 |
| title | Identification and characterization of a novel nanobody against duck hepatitis A virus type 1 |
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