The ubiquitin specific protease USP34 protects the ubiquitin ligase gp78 from proteasomal degradation

The E3 ubiquitin (Ub) ligase gp78 plays an important role in endoplasmic reticulum (ER)-associated degradation (ERAD) and regulation of lipid biogenesis. Although a variety of substrates of gp78 have been described, the regulation of the degradation of gp78 itself remains poorly understood. To address this problem, we used co-immunoprecipitation-coupled liquid chromatography-tandem mass spectrometry (Co-IP/LC-MS/MS) to identify novel proteins interacting with gp78. One of the proteins identified in this study is the deubiquitylating (DUB) enzyme USP34 (Ub-specific protease 34). We demonstrate that knockdown of USP34 facilitates proteasomal degradation of gp78 and consequently impairs the function of gp78 in regulating lipid droplet formation. This study unveils a previously unknown function of USP34 in regulating the metabolic stability of gp78 and adds to our understanding of the relevance of partnering of DUBs and E3s in regulation of protein ubiquitylation. •Deubiquitylating (DUB) enzyme USP34 interacts with the E3 ubiquitin ligase gp78.•USP34 protects gp78 from proteasomal degradation.•Knockdown of gp78 impairs the function of gp78 in regulating lipid droplet formation.•Partnering of DUBs and E3s may be a fine-tuning mechanism regulating ubiquitylation.