The effects of CCR7 and related signaling pathways on Leishmania major ‐infected human dendritic cells
Objectives In our research, we aimed to investigate the roles of CC‐chemokine receptor 7 (CCR7) and relevant signaling pathways in Leishmania major‐infected human dendritic cells (DCs). Methods Differentially expressed genes (DEGs) in L. major‐infected human DCs were selected out and visualized usin...
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| Veröffentlicht in: | Journal of cellular physiology 2019-08, Vol.234 (8), p.13145-13156 |
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| creator | Zhao, Yumin Dang, Zhisheng Wei, Riming Gui, Weifeng Zhang, Yishu Chong, Shigui |
| description | Objectives
In our research, we aimed to investigate the roles of CC‐chemokine receptor 7 (CCR7) and relevant signaling pathways in
Leishmania major‐infected human dendritic cells (DCs).
Methods
Differentially expressed genes (DEGs) in L. major‐infected human DCs were selected out and visualized using R program. Kyoto Encyclopedia of Genes and Genomes pathway analysis was conducted for investigation of significantly enriched signaling pathways and Gene Ontology enrichment analysis was carried out for the unveiling of enriched Molecular Functions and Biological Processes in
L. major‐infected human DCs. Besides, Hub gene was screened out using weighted gene coexpression network analysis and Cytoscape. In addition, enzyme‐linked immunosorbent assay and real‐time quantitative polymerase chain reaction were used for detection of relative expression of
CCR7, interleukin‐12 (IL‐12), and interferon‐γ (IFN‐γ) in
L. major‐infected human DCs and western blot analysis was used for detection of relative expression of CCR7 and other proteins in JAK‐STAT signaling pathway in
L. major‐infected human DCs.
Results
CCR7 was upregulated and both chemokine and JAK‐STAT signaling pathway were activated in
L. major‐infected human DCs. During the
L. major infection, total number of
L. major‐infected human DCs were increased, as well as the relative expression levels of
CCR7, IL‐12, and IFN‐γ and proteins in the JAK‐STAT signaling pathway. Overexpression of
CCR7 not only increased expression levels of IL‐12 and IFN‐γ but also activated the JAK‐STAT signaling pathway to affect the leishmaniasis progression.
Conclusion
L. major infection‐induced activation of
CCR7, as well as
JAK2 and
STAT1, might well upregulate the expression of
BAX yet suppress the expression of both
Bcl2 and
c‐Jun to affect leishmaniasis progression.
1. Our study showed that CCR7 was up‐regulated and both chemokine and JAK‐STAT signaling pathways were activated in Leishmaniasis. 2. Down‐regulation of CCR7 and inactivation of JAK‐STAT signaling pathway may suppress Leishmaniasis progression in the future. |
| doi_str_mv | 10.1002/jcp.27985 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2160366610</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2211498562</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3535-fa0a8fc97014afa9b2502bb388cb42c251a5ba04056ddde7556ad9f85bc3e7df3</originalsourceid><addsrcrecordid>eNp1kElO5DAUQC1EC4phwQWQJTawCHiInWSJIoZGJXULwTr68UBcylDYiUq14wh9Rk7SLgp60RIrL_77T98PoRNKLikh7GqhlpcsK3Kxg2aUFFmSSsF20SzOaFKIlO6jgxAWhJCi4HwP7XMi8lTKbIaap8ZgY61RY8CDxWX5mGHoNfamhdFoHNxLD63rX_ASxmYF64j1eG5caDroHeAOFoPH729_XL-xxJVmihOsTa-9G53CyrRtOEI_LLTBHH--h-j59uapvE_mv-5-ltfzRHHBRWKBQG5VkRGagoWiZoKwuuZ5ruqUKSYoiBpISoTUWptMCAm6sLmoFTeZtvwQnW-9Sz-8TiaMVefC5gLozTCFilFJuJSSkoie_YcuhsnH30aKUZrGoJJF6mJLKT-E4I2tlt514NcVJdUmfxXzVx_5I3v6aZzqzuh_5FfvCFxtgZVrzfp7U_VQ_t4q_wKXHI-b</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2211498562</pqid></control><display><type>article</type><title>The effects of CCR7 and related signaling pathways on Leishmania major ‐infected human dendritic cells</title><source>MEDLINE</source><source>Wiley Online Library All Journals</source><creator>Zhao, Yumin ; Dang, Zhisheng ; Wei, Riming ; Gui, Weifeng ; Zhang, Yishu ; Chong, Shigui</creator><creatorcontrib>Zhao, Yumin ; Dang, Zhisheng ; Wei, Riming ; Gui, Weifeng ; Zhang, Yishu ; Chong, Shigui</creatorcontrib><description>Objectives
In our research, we aimed to investigate the roles of CC‐chemokine receptor 7 (CCR7) and relevant signaling pathways in
Leishmania major‐infected human dendritic cells (DCs).
Methods
Differentially expressed genes (DEGs) in L. major‐infected human DCs were selected out and visualized using R program. Kyoto Encyclopedia of Genes and Genomes pathway analysis was conducted for investigation of significantly enriched signaling pathways and Gene Ontology enrichment analysis was carried out for the unveiling of enriched Molecular Functions and Biological Processes in
L. major‐infected human DCs. Besides, Hub gene was screened out using weighted gene coexpression network analysis and Cytoscape. In addition, enzyme‐linked immunosorbent assay and real‐time quantitative polymerase chain reaction were used for detection of relative expression of
CCR7, interleukin‐12 (IL‐12), and interferon‐γ (IFN‐γ) in
L. major‐infected human DCs and western blot analysis was used for detection of relative expression of CCR7 and other proteins in JAK‐STAT signaling pathway in
L. major‐infected human DCs.
Results
CCR7 was upregulated and both chemokine and JAK‐STAT signaling pathway were activated in
L. major‐infected human DCs. During the
L. major infection, total number of
L. major‐infected human DCs were increased, as well as the relative expression levels of
CCR7, IL‐12, and IFN‐γ and proteins in the JAK‐STAT signaling pathway. Overexpression of
CCR7 not only increased expression levels of IL‐12 and IFN‐γ but also activated the JAK‐STAT signaling pathway to affect the leishmaniasis progression.
Conclusion
L. major infection‐induced activation of
CCR7, as well as
JAK2 and
STAT1, might well upregulate the expression of
BAX yet suppress the expression of both
Bcl2 and
c‐Jun to affect leishmaniasis progression.
1. Our study showed that CCR7 was up‐regulated and both chemokine and JAK‐STAT signaling pathways were activated in Leishmaniasis. 2. Down‐regulation of CCR7 and inactivation of JAK‐STAT signaling pathway may suppress Leishmaniasis progression in the future.</description><identifier>ISSN: 0021-9541</identifier><identifier>EISSN: 1097-4652</identifier><identifier>DOI: 10.1002/jcp.27985</identifier><identifier>PMID: 30584667</identifier><language>eng</language><publisher>United States: Wiley Subscription Services, Inc</publisher><subject>BAX protein ; Biological activity ; CC chemokine receptors ; CCR7 ; CCR7 protein ; Chemokines ; Dendritic cells ; Dendritic Cells - immunology ; Dendritic Cells - metabolism ; Encyclopedias ; Enrichment ; Genes ; Genomes ; human dendritic cells ; Humans ; Interferon ; Interleukins ; JAK‐STAT signaling pathway ; Janus kinase 2 ; Janus Kinases - immunology ; Janus Kinases - metabolism ; Leishmania major ; Leishmaniasis ; Leishmaniasis, Cutaneous - immunology ; Leishmaniasis, Cutaneous - metabolism ; Network analysis ; Parasitic diseases ; Polymerase chain reaction ; Proteins ; Receptors, CCR7 - immunology ; Receptors, CCR7 - metabolism ; Signal transduction ; Signal Transduction - physiology ; Signaling ; STAT Transcription Factors - immunology ; STAT Transcription Factors - metabolism ; Stat1 protein ; Vector-borne diseases</subject><ispartof>Journal of cellular physiology, 2019-08, Vol.234 (8), p.13145-13156</ispartof><rights>2018 Wiley Periodicals, Inc.</rights><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3535-fa0a8fc97014afa9b2502bb388cb42c251a5ba04056ddde7556ad9f85bc3e7df3</citedby><cites>FETCH-LOGICAL-c3535-fa0a8fc97014afa9b2502bb388cb42c251a5ba04056ddde7556ad9f85bc3e7df3</cites><orcidid>0000-0003-3525-3588</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjcp.27985$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjcp.27985$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30584667$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhao, Yumin</creatorcontrib><creatorcontrib>Dang, Zhisheng</creatorcontrib><creatorcontrib>Wei, Riming</creatorcontrib><creatorcontrib>Gui, Weifeng</creatorcontrib><creatorcontrib>Zhang, Yishu</creatorcontrib><creatorcontrib>Chong, Shigui</creatorcontrib><title>The effects of CCR7 and related signaling pathways on Leishmania major ‐infected human dendritic cells</title><title>Journal of cellular physiology</title><addtitle>J Cell Physiol</addtitle><description>Objectives
In our research, we aimed to investigate the roles of CC‐chemokine receptor 7 (CCR7) and relevant signaling pathways in
Leishmania major‐infected human dendritic cells (DCs).
Methods
Differentially expressed genes (DEGs) in L. major‐infected human DCs were selected out and visualized using R program. Kyoto Encyclopedia of Genes and Genomes pathway analysis was conducted for investigation of significantly enriched signaling pathways and Gene Ontology enrichment analysis was carried out for the unveiling of enriched Molecular Functions and Biological Processes in
L. major‐infected human DCs. Besides, Hub gene was screened out using weighted gene coexpression network analysis and Cytoscape. In addition, enzyme‐linked immunosorbent assay and real‐time quantitative polymerase chain reaction were used for detection of relative expression of
CCR7, interleukin‐12 (IL‐12), and interferon‐γ (IFN‐γ) in
L. major‐infected human DCs and western blot analysis was used for detection of relative expression of CCR7 and other proteins in JAK‐STAT signaling pathway in
L. major‐infected human DCs.
Results
CCR7 was upregulated and both chemokine and JAK‐STAT signaling pathway were activated in
L. major‐infected human DCs. During the
L. major infection, total number of
L. major‐infected human DCs were increased, as well as the relative expression levels of
CCR7, IL‐12, and IFN‐γ and proteins in the JAK‐STAT signaling pathway. Overexpression of
CCR7 not only increased expression levels of IL‐12 and IFN‐γ but also activated the JAK‐STAT signaling pathway to affect the leishmaniasis progression.
Conclusion
L. major infection‐induced activation of
CCR7, as well as
JAK2 and
STAT1, might well upregulate the expression of
BAX yet suppress the expression of both
Bcl2 and
c‐Jun to affect leishmaniasis progression.
1. Our study showed that CCR7 was up‐regulated and both chemokine and JAK‐STAT signaling pathways were activated in Leishmaniasis. 2. Down‐regulation of CCR7 and inactivation of JAK‐STAT signaling pathway may suppress Leishmaniasis progression in the future.</description><subject>BAX protein</subject><subject>Biological activity</subject><subject>CC chemokine receptors</subject><subject>CCR7</subject><subject>CCR7 protein</subject><subject>Chemokines</subject><subject>Dendritic cells</subject><subject>Dendritic Cells - immunology</subject><subject>Dendritic Cells - metabolism</subject><subject>Encyclopedias</subject><subject>Enrichment</subject><subject>Genes</subject><subject>Genomes</subject><subject>human dendritic cells</subject><subject>Humans</subject><subject>Interferon</subject><subject>Interleukins</subject><subject>JAK‐STAT signaling pathway</subject><subject>Janus kinase 2</subject><subject>Janus Kinases - immunology</subject><subject>Janus Kinases - metabolism</subject><subject>Leishmania major</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis, Cutaneous - immunology</subject><subject>Leishmaniasis, Cutaneous - metabolism</subject><subject>Network analysis</subject><subject>Parasitic diseases</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Receptors, CCR7 - immunology</subject><subject>Receptors, CCR7 - metabolism</subject><subject>Signal transduction</subject><subject>Signal Transduction - physiology</subject><subject>Signaling</subject><subject>STAT Transcription Factors - immunology</subject><subject>STAT Transcription Factors - metabolism</subject><subject>Stat1 protein</subject><subject>Vector-borne diseases</subject><issn>0021-9541</issn><issn>1097-4652</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kElO5DAUQC1EC4phwQWQJTawCHiInWSJIoZGJXULwTr68UBcylDYiUq14wh9Rk7SLgp60RIrL_77T98PoRNKLikh7GqhlpcsK3Kxg2aUFFmSSsF20SzOaFKIlO6jgxAWhJCi4HwP7XMi8lTKbIaap8ZgY61RY8CDxWX5mGHoNfamhdFoHNxLD63rX_ASxmYF64j1eG5caDroHeAOFoPH729_XL-xxJVmihOsTa-9G53CyrRtOEI_LLTBHH--h-j59uapvE_mv-5-ltfzRHHBRWKBQG5VkRGagoWiZoKwuuZ5ruqUKSYoiBpISoTUWptMCAm6sLmoFTeZtvwQnW-9Sz-8TiaMVefC5gLozTCFilFJuJSSkoie_YcuhsnH30aKUZrGoJJF6mJLKT-E4I2tlt514NcVJdUmfxXzVx_5I3v6aZzqzuh_5FfvCFxtgZVrzfp7U_VQ_t4q_wKXHI-b</recordid><startdate>201908</startdate><enddate>201908</enddate><creator>Zhao, Yumin</creator><creator>Dang, Zhisheng</creator><creator>Wei, Riming</creator><creator>Gui, Weifeng</creator><creator>Zhang, Yishu</creator><creator>Chong, Shigui</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7U7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3525-3588</orcidid></search><sort><creationdate>201908</creationdate><title>The effects of CCR7 and related signaling pathways on Leishmania major ‐infected human dendritic cells</title><author>Zhao, Yumin ; Dang, Zhisheng ; Wei, Riming ; Gui, Weifeng ; Zhang, Yishu ; Chong, Shigui</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3535-fa0a8fc97014afa9b2502bb388cb42c251a5ba04056ddde7556ad9f85bc3e7df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>BAX protein</topic><topic>Biological activity</topic><topic>CC chemokine receptors</topic><topic>CCR7</topic><topic>CCR7 protein</topic><topic>Chemokines</topic><topic>Dendritic cells</topic><topic>Dendritic Cells - immunology</topic><topic>Dendritic Cells - metabolism</topic><topic>Encyclopedias</topic><topic>Enrichment</topic><topic>Genes</topic><topic>Genomes</topic><topic>human dendritic cells</topic><topic>Humans</topic><topic>Interferon</topic><topic>Interleukins</topic><topic>JAK‐STAT signaling pathway</topic><topic>Janus kinase 2</topic><topic>Janus Kinases - immunology</topic><topic>Janus Kinases - metabolism</topic><topic>Leishmania major</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis, Cutaneous - immunology</topic><topic>Leishmaniasis, Cutaneous - metabolism</topic><topic>Network analysis</topic><topic>Parasitic diseases</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Receptors, CCR7 - immunology</topic><topic>Receptors, CCR7 - metabolism</topic><topic>Signal transduction</topic><topic>Signal Transduction - physiology</topic><topic>Signaling</topic><topic>STAT Transcription Factors - immunology</topic><topic>STAT Transcription Factors - metabolism</topic><topic>Stat1 protein</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhao, Yumin</creatorcontrib><creatorcontrib>Dang, Zhisheng</creatorcontrib><creatorcontrib>Wei, Riming</creatorcontrib><creatorcontrib>Gui, Weifeng</creatorcontrib><creatorcontrib>Zhang, Yishu</creatorcontrib><creatorcontrib>Chong, Shigui</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cellular physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhao, Yumin</au><au>Dang, Zhisheng</au><au>Wei, Riming</au><au>Gui, Weifeng</au><au>Zhang, Yishu</au><au>Chong, Shigui</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The effects of CCR7 and related signaling pathways on Leishmania major ‐infected human dendritic cells</atitle><jtitle>Journal of cellular physiology</jtitle><addtitle>J Cell Physiol</addtitle><date>2019-08</date><risdate>2019</risdate><volume>234</volume><issue>8</issue><spage>13145</spage><epage>13156</epage><pages>13145-13156</pages><issn>0021-9541</issn><eissn>1097-4652</eissn><abstract>Objectives
In our research, we aimed to investigate the roles of CC‐chemokine receptor 7 (CCR7) and relevant signaling pathways in
Leishmania major‐infected human dendritic cells (DCs).
Methods
Differentially expressed genes (DEGs) in L. major‐infected human DCs were selected out and visualized using R program. Kyoto Encyclopedia of Genes and Genomes pathway analysis was conducted for investigation of significantly enriched signaling pathways and Gene Ontology enrichment analysis was carried out for the unveiling of enriched Molecular Functions and Biological Processes in
L. major‐infected human DCs. Besides, Hub gene was screened out using weighted gene coexpression network analysis and Cytoscape. In addition, enzyme‐linked immunosorbent assay and real‐time quantitative polymerase chain reaction were used for detection of relative expression of
CCR7, interleukin‐12 (IL‐12), and interferon‐γ (IFN‐γ) in
L. major‐infected human DCs and western blot analysis was used for detection of relative expression of CCR7 and other proteins in JAK‐STAT signaling pathway in
L. major‐infected human DCs.
Results
CCR7 was upregulated and both chemokine and JAK‐STAT signaling pathway were activated in
L. major‐infected human DCs. During the
L. major infection, total number of
L. major‐infected human DCs were increased, as well as the relative expression levels of
CCR7, IL‐12, and IFN‐γ and proteins in the JAK‐STAT signaling pathway. Overexpression of
CCR7 not only increased expression levels of IL‐12 and IFN‐γ but also activated the JAK‐STAT signaling pathway to affect the leishmaniasis progression.
Conclusion
L. major infection‐induced activation of
CCR7, as well as
JAK2 and
STAT1, might well upregulate the expression of
BAX yet suppress the expression of both
Bcl2 and
c‐Jun to affect leishmaniasis progression.
1. Our study showed that CCR7 was up‐regulated and both chemokine and JAK‐STAT signaling pathways were activated in Leishmaniasis. 2. Down‐regulation of CCR7 and inactivation of JAK‐STAT signaling pathway may suppress Leishmaniasis progression in the future.</abstract><cop>United States</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30584667</pmid><doi>10.1002/jcp.27985</doi><tpages>12</tpages><orcidid>https://orcid.org/0000-0003-3525-3588</orcidid></addata></record> |
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| source | MEDLINE; Wiley Online Library All Journals |
| subjects | BAX protein Biological activity CC chemokine receptors CCR7 CCR7 protein Chemokines Dendritic cells Dendritic Cells - immunology Dendritic Cells - metabolism Encyclopedias Enrichment Genes Genomes human dendritic cells Humans Interferon Interleukins JAK‐STAT signaling pathway Janus kinase 2 Janus Kinases - immunology Janus Kinases - metabolism Leishmania major Leishmaniasis Leishmaniasis, Cutaneous - immunology Leishmaniasis, Cutaneous - metabolism Network analysis Parasitic diseases Polymerase chain reaction Proteins Receptors, CCR7 - immunology Receptors, CCR7 - metabolism Signal transduction Signal Transduction - physiology Signaling STAT Transcription Factors - immunology STAT Transcription Factors - metabolism Stat1 protein Vector-borne diseases |
| title | The effects of CCR7 and related signaling pathways on Leishmania major ‐infected human dendritic cells |
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