Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato
The aim of this work was to evaluate the possibility of control of wilt disease caused by Fusarium andiyazi through chitosan (CS) and chitosan nanoparticles (CNPs). In the present study, the expression pattern of pathogenesis-related (PR) proteins genes such as PR-1, PR-2 (β-1,3-glucanase), PR-8 (ch...
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| Veröffentlicht in: | International journal of biological macromolecules 2019-03, Vol.125, p.948-954 |
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| description | The aim of this work was to evaluate the possibility of control of wilt disease caused by Fusarium andiyazi through chitosan (CS) and chitosan nanoparticles (CNPs). In the present study, the expression pattern of pathogenesis-related (PR) proteins genes such as PR-1, PR-2 (β-1,3-glucanase), PR-8 (chitinase), and PR-10 was analyzed using real-time RT-PCR. In vitro studies showed that among different concentrations (0.1–5.0 mg/ml), 5.0 mg/ml concentration of CS and CNPs produced maximum inhibition of radial mycelial growth, 54.8% and 73.81%, respectively. Also, upregulated expression of β-1,3-glucanase, chitinase, PR-1 and PR-10 genes were recorded with 1.48, 1.15, 1.15, and 1.41, fold expression in 24 hpi, respectively, in plants inoculated with CNPs. The most significant up-regulation was observed in transcript profile of SOD that showed 4.5-foldexpression, at 48 hpi. Therefore, our results confirmed that CS and CNPs induced up-regulation of PR-proteins and antioxidant genes might play a significant role for successful biocontrol.
•Chitosan and chitosan nanoparticles showed antifungal activities against phytopathogenic fungi, Fusarium andiyazi.•Antifungal activity of chitosan nanoparticles was higher than the chitosan.•Chitinases and β-1,3-glucanases genes were up-regulated by 1.15 and 1.48-fold, respectively.•The most significant up-regulation (4.5 fold) was observed in transcript profile of SOD. |
| doi_str_mv | 10.1016/j.ijbiomac.2018.12.167 |
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•Chitosan and chitosan nanoparticles showed antifungal activities against phytopathogenic fungi, Fusarium andiyazi.•Antifungal activity of chitosan nanoparticles was higher than the chitosan.•Chitinases and β-1,3-glucanases genes were up-regulated by 1.15 and 1.48-fold, respectively.•The most significant up-regulation (4.5 fold) was observed in transcript profile of SOD.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2018.12.167</identifier><identifier>PMID: 30576730</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Antioxidant enzymes ; Chitinase ; Chitinases - genetics ; Chitinases - immunology ; Chitosan ; Chitosan - chemistry ; Chitosan - pharmacology ; Chitosan nanoparticles ; Enzyme Activation - drug effects ; Fusarium - drug effects ; Fusarium - growth & development ; Fusarium - pathogenicity ; Fusarium andiyazi ; Gene Expression Regulation, Plant ; Glucan 1,3-beta-Glucosidase - genetics ; Glucan 1,3-beta-Glucosidase - immunology ; Host-Pathogen Interactions ; Lycopersicon esculentum - drug effects ; Lycopersicon esculentum - genetics ; Lycopersicon esculentum - immunology ; Lycopersicon esculentum - microbiology ; Mycelium - drug effects ; Mycelium - growth & development ; Mycelium - pathogenicity ; Nanoparticles - chemistry ; Pathogenesis-related proteins ; Plant Diseases - genetics ; Plant Diseases - immunology ; Plant Diseases - microbiology ; Plant Proteins - agonists ; Plant Proteins - genetics ; Plant Proteins - immunology ; Stress, Physiological - drug effects ; Stress, Physiological - immunology ; Superoxide Dismutase - genetics ; Superoxide Dismutase - immunology ; β-1,3-Glucanase</subject><ispartof>International journal of biological macromolecules, 2019-03, Vol.125, p.948-954</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-7d3eaa450639ce56894c5d6d9ca6d67ef1531a60233f7594821385e87644b653</citedby><cites>FETCH-LOGICAL-c368t-7d3eaa450639ce56894c5d6d9ca6d67ef1531a60233f7594821385e87644b653</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0141813018363815$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30576730$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chun, Se-Chul</creatorcontrib><creatorcontrib>Chandrasekaran, Murugesan</creatorcontrib><title>Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>The aim of this work was to evaluate the possibility of control of wilt disease caused by Fusarium andiyazi through chitosan (CS) and chitosan nanoparticles (CNPs). In the present study, the expression pattern of pathogenesis-related (PR) proteins genes such as PR-1, PR-2 (β-1,3-glucanase), PR-8 (chitinase), and PR-10 was analyzed using real-time RT-PCR. In vitro studies showed that among different concentrations (0.1–5.0 mg/ml), 5.0 mg/ml concentration of CS and CNPs produced maximum inhibition of radial mycelial growth, 54.8% and 73.81%, respectively. Also, upregulated expression of β-1,3-glucanase, chitinase, PR-1 and PR-10 genes were recorded with 1.48, 1.15, 1.15, and 1.41, fold expression in 24 hpi, respectively, in plants inoculated with CNPs. The most significant up-regulation was observed in transcript profile of SOD that showed 4.5-foldexpression, at 48 hpi. Therefore, our results confirmed that CS and CNPs induced up-regulation of PR-proteins and antioxidant genes might play a significant role for successful biocontrol.
•Chitosan and chitosan nanoparticles showed antifungal activities against phytopathogenic fungi, Fusarium andiyazi.•Antifungal activity of chitosan nanoparticles was higher than the chitosan.•Chitinases and β-1,3-glucanases genes were up-regulated by 1.15 and 1.48-fold, respectively.•The most significant up-regulation (4.5 fold) was observed in transcript profile of SOD.</description><subject>Antioxidant enzymes</subject><subject>Chitinase</subject><subject>Chitinases - genetics</subject><subject>Chitinases - immunology</subject><subject>Chitosan</subject><subject>Chitosan - chemistry</subject><subject>Chitosan - pharmacology</subject><subject>Chitosan nanoparticles</subject><subject>Enzyme Activation - drug effects</subject><subject>Fusarium - drug effects</subject><subject>Fusarium - growth & development</subject><subject>Fusarium - pathogenicity</subject><subject>Fusarium andiyazi</subject><subject>Gene Expression Regulation, Plant</subject><subject>Glucan 1,3-beta-Glucosidase - genetics</subject><subject>Glucan 1,3-beta-Glucosidase - immunology</subject><subject>Host-Pathogen Interactions</subject><subject>Lycopersicon esculentum - drug effects</subject><subject>Lycopersicon esculentum - genetics</subject><subject>Lycopersicon esculentum - immunology</subject><subject>Lycopersicon esculentum - microbiology</subject><subject>Mycelium - drug effects</subject><subject>Mycelium - growth & development</subject><subject>Mycelium - pathogenicity</subject><subject>Nanoparticles - chemistry</subject><subject>Pathogenesis-related proteins</subject><subject>Plant Diseases - genetics</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - microbiology</subject><subject>Plant Proteins - agonists</subject><subject>Plant Proteins - genetics</subject><subject>Plant Proteins - immunology</subject><subject>Stress, Physiological - drug effects</subject><subject>Stress, Physiological - immunology</subject><subject>Superoxide Dismutase - genetics</subject><subject>Superoxide Dismutase - immunology</subject><subject>β-1,3-Glucanase</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcGOFCEQholZ4467vsKGo5duoWlo-qaZ7KrJJl72Thio3mHSDS3FGH0DH1vG2fHqCYr66q-ifkLuOGs54-rDoQ2HXUiLdW3HuG5513I1vCIbroexYYyJK7JhvOeN5oJdk7eIh_qqJNdvyLVgclCDYBvye7sPJaGN1EZP3SWINqbV5hLcDEhD9EcHnsLPNQNiSJGmia627NMzRMCATYbZloqsORUIEenfBIW4t9HVS521ilEsJwFa0gz5lKjSNVhsSbfk9WRnhHcv5w15erh_2n5pHr99_rr99Ng4oXRpBi_A2l4yJUYHUumxd9IrPzqrvBpg4lJwq1gnxDTIsdcdF1qCHlTf75QUN-T9WbYO-v0IWMwS0ME82wjpiKbjchy11EpUVJ1RlxNihsmsOSw2_zKcmZMJ5mAuJpiTCYZ3pppQC-9eehx3C_h_ZZetV-DjGYD60R8BskEXoK7DhwyuGJ_C_3r8ARM2ntU</recordid><startdate>20190315</startdate><enddate>20190315</enddate><creator>Chun, Se-Chul</creator><creator>Chandrasekaran, Murugesan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20190315</creationdate><title>Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato</title><author>Chun, Se-Chul ; Chandrasekaran, Murugesan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-7d3eaa450639ce56894c5d6d9ca6d67ef1531a60233f7594821385e87644b653</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Antioxidant enzymes</topic><topic>Chitinase</topic><topic>Chitinases - genetics</topic><topic>Chitinases - immunology</topic><topic>Chitosan</topic><topic>Chitosan - chemistry</topic><topic>Chitosan - pharmacology</topic><topic>Chitosan nanoparticles</topic><topic>Enzyme Activation - drug effects</topic><topic>Fusarium - drug effects</topic><topic>Fusarium - growth & development</topic><topic>Fusarium - pathogenicity</topic><topic>Fusarium andiyazi</topic><topic>Gene Expression Regulation, Plant</topic><topic>Glucan 1,3-beta-Glucosidase - genetics</topic><topic>Glucan 1,3-beta-Glucosidase - immunology</topic><topic>Host-Pathogen Interactions</topic><topic>Lycopersicon esculentum - drug effects</topic><topic>Lycopersicon esculentum - genetics</topic><topic>Lycopersicon esculentum - immunology</topic><topic>Lycopersicon esculentum - microbiology</topic><topic>Mycelium - drug effects</topic><topic>Mycelium - growth & development</topic><topic>Mycelium - pathogenicity</topic><topic>Nanoparticles - chemistry</topic><topic>Pathogenesis-related proteins</topic><topic>Plant Diseases - genetics</topic><topic>Plant Diseases - immunology</topic><topic>Plant Diseases - microbiology</topic><topic>Plant Proteins - agonists</topic><topic>Plant Proteins - genetics</topic><topic>Plant Proteins - immunology</topic><topic>Stress, Physiological - drug effects</topic><topic>Stress, Physiological - immunology</topic><topic>Superoxide Dismutase - genetics</topic><topic>Superoxide Dismutase - immunology</topic><topic>β-1,3-Glucanase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chun, Se-Chul</creatorcontrib><creatorcontrib>Chandrasekaran, Murugesan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chun, Se-Chul</au><au>Chandrasekaran, Murugesan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2019-03-15</date><risdate>2019</risdate><volume>125</volume><spage>948</spage><epage>954</epage><pages>948-954</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>The aim of this work was to evaluate the possibility of control of wilt disease caused by Fusarium andiyazi through chitosan (CS) and chitosan nanoparticles (CNPs). In the present study, the expression pattern of pathogenesis-related (PR) proteins genes such as PR-1, PR-2 (β-1,3-glucanase), PR-8 (chitinase), and PR-10 was analyzed using real-time RT-PCR. In vitro studies showed that among different concentrations (0.1–5.0 mg/ml), 5.0 mg/ml concentration of CS and CNPs produced maximum inhibition of radial mycelial growth, 54.8% and 73.81%, respectively. Also, upregulated expression of β-1,3-glucanase, chitinase, PR-1 and PR-10 genes were recorded with 1.48, 1.15, 1.15, and 1.41, fold expression in 24 hpi, respectively, in plants inoculated with CNPs. The most significant up-regulation was observed in transcript profile of SOD that showed 4.5-foldexpression, at 48 hpi. Therefore, our results confirmed that CS and CNPs induced up-regulation of PR-proteins and antioxidant genes might play a significant role for successful biocontrol.
•Chitosan and chitosan nanoparticles showed antifungal activities against phytopathogenic fungi, Fusarium andiyazi.•Antifungal activity of chitosan nanoparticles was higher than the chitosan.•Chitinases and β-1,3-glucanases genes were up-regulated by 1.15 and 1.48-fold, respectively.•The most significant up-regulation (4.5 fold) was observed in transcript profile of SOD.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30576730</pmid><doi>10.1016/j.ijbiomac.2018.12.167</doi><tpages>7</tpages></addata></record> |
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| subjects | Antioxidant enzymes Chitinase Chitinases - genetics Chitinases - immunology Chitosan Chitosan - chemistry Chitosan - pharmacology Chitosan nanoparticles Enzyme Activation - drug effects Fusarium - drug effects Fusarium - growth & development Fusarium - pathogenicity Fusarium andiyazi Gene Expression Regulation, Plant Glucan 1,3-beta-Glucosidase - genetics Glucan 1,3-beta-Glucosidase - immunology Host-Pathogen Interactions Lycopersicon esculentum - drug effects Lycopersicon esculentum - genetics Lycopersicon esculentum - immunology Lycopersicon esculentum - microbiology Mycelium - drug effects Mycelium - growth & development Mycelium - pathogenicity Nanoparticles - chemistry Pathogenesis-related proteins Plant Diseases - genetics Plant Diseases - immunology Plant Diseases - microbiology Plant Proteins - agonists Plant Proteins - genetics Plant Proteins - immunology Stress, Physiological - drug effects Stress, Physiological - immunology Superoxide Dismutase - genetics Superoxide Dismutase - immunology β-1,3-Glucanase |
| title | Chitosan and chitosan nanoparticles induced expression of pathogenesis-related proteins genes enhances biotic stress tolerance in tomato |
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