Nucleocytoplasmic shuttling of the human parainfluenza virus type 2 phosphoprotein

Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein i...

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Veröffentlicht in:Virology (New York, N.Y.) N.Y.), 2019-02, Vol.528, p.54-63
Hauptverfasser: Ohtsuka, Junpei, Matsumoto, Yusuke, Ohta, Keisuke, Fukumura, Masayuki, Tsurudome, Masato, Nosaka, Tetsuya, Nishio, Machiko
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Sprache:eng
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Zusammenfassung:Human parainfluenza virus type 2 phosphoprotein (P) is an essential component of viral polymerase. The P gene encodes both P and accessory V proteins by a specific gene editing mechanism. Therefore, the N-terminal 164 amino acids of P protein are common to V protein. Interestingly, while P protein is located in the cytoplasm, V protein is found mainly in the nucleus. Using deletion mutants, we show the presence of a nuclear localization signal (NLS) in the P/V common domain, and a nuclear export signal (NES) in the C-terminal P specific region. The NLS region makes a complex with importin α5 or 7. In the presence of leptomycin B, P protein is retained in the nucleus, indicating that it contains a CRM1-dependent NES. We identified the NLS (65PVKPRRKK72) and the NES (225IIELLKGLDL234) using β-galactosidase fusion proteins. Moreover, nucleocytoplasmic shuttling of P protein appears to be important for efficient viral polymerase activity.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2018.12.005