Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy
Palyno‐anatomical study of monocots taxa using Light and Scanning Electron Microscopy (SEM) was first time conducted with a view to evaluating their taxonomic significance. Studied plants were collected from different eco‐climatic zones of Pakistan ranges from tropical, sub‐tropical, and moist habit...
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creator | Bahadur, Saraj Ahmad, Mushtaq Zafar, Muhammad Sultana, Shazia Begum, Najla Ashfaq, Shomaila Gul, Saba Khan, Muhammad Saleem Shah, Syed Nasar Ullah, Fazal Saqib, Saddam Ayaz, Asma |
description | Palyno‐anatomical study of monocots taxa using Light and Scanning Electron Microscopy (SEM) was first time conducted with a view to evaluating their taxonomic significance. Studied plants were collected from different eco‐climatic zones of Pakistan ranges from tropical, sub‐tropical, and moist habitats. The aim of this study is to use palyno‐anatomical features for the correct identification, systematic comparison, and investigation to elucidate the taxonomic significance of these features, which are useful to taxonomists for identifying monocot taxa. A signification variation was observed in quantitative and qualitative characters by using the standard protocol of light microscopy (LM) and SEM. Epidermal cell length varied from maximum in Allium griffthianum (480 ± 35.9) μm at the adaxial surface to minimum in Canna indica (33.6 ± 8.53) μm on abaxial surface. Maximum exine thickness was observed in Canna indica (4.46) μm and minimum in Allium grifthianum (0.8) μm. Variation was observed in shape and exine ornamentation of the pollen, shape of the epidermal cell, number, size, and type of stomata, guard cell shape, and anticlinal wall pattern. Based on these palyno‐anatomical features a taxonomic key was developed, which help in the discrimination of studied taxa. In conclusion, LM and SEM pollen and epidermal morphology is explanatory, significant, and can be of special interest for the plant taxonomist in the correct identification of monocots taxa.
The SEM and LM method use as a tool in the identification of monocot taxa.
Palyno‐anatomical features were first time documented for its taxonomic significance.
Based on these features, a taxonomic key was developed for the correct identification and delimitation of the monocot taxa. |
doi_str_mv | 10.1002/jemt.23179 |
format | Article |
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The SEM and LM method use as a tool in the identification of monocot taxa.
Palyno‐anatomical features were first time documented for its taxonomic significance.
Based on these features, a taxonomic key was developed for the correct identification and delimitation of the monocot taxa.</description><identifier>ISSN: 1059-910X</identifier><identifier>EISSN: 1097-0029</identifier><identifier>DOI: 10.1002/jemt.23179</identifier><identifier>PMID: 30575189</identifier><language>eng</language><publisher>Hoboken, USA: John Wiley & Sons, Inc</publisher><subject>Allium ; Amaryllidaceae - anatomy & histology ; Amaryllidaceae - classification ; Anatomy ; Araceae - anatomy & histology ; Araceae - classification ; Asparagaceae - anatomy & histology ; Asparagaceae - classification ; Canna indica ; Cell size ; Climatic zones ; Ecosystem ; Epidermal Cells - ultrastructure ; light and scanning electron microscopy ; Light microscopy ; Liliaceae - anatomy & histology ; Liliaceae - classification ; Microscopy, Electron, Scanning ; monocot ; Morphology ; Pakistan ; palyno‐anatomical features ; Plant Epidermis - ultrastructure ; Pollen ; Pollen - ultrastructure ; Scanning electron microscopy ; Stomata ; Taxa ; Taxonomic significance ; Taxonomy</subject><ispartof>Microscopy research and technique, 2019-04, Vol.82 (4), p.373-393</ispartof><rights>2019 Wiley Periodicals, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3579-664c4c08a56ad86ed4a7087ac88272a8a31709e54bb4da3755ef0bf3a0de59c03</citedby><cites>FETCH-LOGICAL-c3579-664c4c08a56ad86ed4a7087ac88272a8a31709e54bb4da3755ef0bf3a0de59c03</cites><orcidid>0000-0002-5496-7861 ; 0000-0002-9002-6110 ; 0000-0002-1240-8949 ; 0000-0003-2971-2848 ; 0000-0003-2002-3907 ; 0000-0001-5856-3189 ; 0000-0002-3378-9509</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjemt.23179$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjemt.23179$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30575189$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bahadur, Saraj</creatorcontrib><creatorcontrib>Ahmad, Mushtaq</creatorcontrib><creatorcontrib>Zafar, Muhammad</creatorcontrib><creatorcontrib>Sultana, Shazia</creatorcontrib><creatorcontrib>Begum, Najla</creatorcontrib><creatorcontrib>Ashfaq, Shomaila</creatorcontrib><creatorcontrib>Gul, Saba</creatorcontrib><creatorcontrib>Khan, Muhammad Saleem</creatorcontrib><creatorcontrib>Shah, Syed Nasar</creatorcontrib><creatorcontrib>Ullah, Fazal</creatorcontrib><creatorcontrib>Saqib, Saddam</creatorcontrib><creatorcontrib>Ayaz, Asma</creatorcontrib><title>Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy</title><title>Microscopy research and technique</title><addtitle>Microsc Res Tech</addtitle><description>Palyno‐anatomical study of monocots taxa using Light and Scanning Electron Microscopy (SEM) was first time conducted with a view to evaluating their taxonomic significance. Studied plants were collected from different eco‐climatic zones of Pakistan ranges from tropical, sub‐tropical, and moist habitats. The aim of this study is to use palyno‐anatomical features for the correct identification, systematic comparison, and investigation to elucidate the taxonomic significance of these features, which are useful to taxonomists for identifying monocot taxa. A signification variation was observed in quantitative and qualitative characters by using the standard protocol of light microscopy (LM) and SEM. Epidermal cell length varied from maximum in Allium griffthianum (480 ± 35.9) μm at the adaxial surface to minimum in Canna indica (33.6 ± 8.53) μm on abaxial surface. Maximum exine thickness was observed in Canna indica (4.46) μm and minimum in Allium grifthianum (0.8) μm. Variation was observed in shape and exine ornamentation of the pollen, shape of the epidermal cell, number, size, and type of stomata, guard cell shape, and anticlinal wall pattern. Based on these palyno‐anatomical features a taxonomic key was developed, which help in the discrimination of studied taxa. In conclusion, LM and SEM pollen and epidermal morphology is explanatory, significant, and can be of special interest for the plant taxonomist in the correct identification of monocots taxa.
The SEM and LM method use as a tool in the identification of monocot taxa.
Palyno‐anatomical features were first time documented for its taxonomic significance.
Based on these features, a taxonomic key was developed for the correct identification and delimitation of the monocot taxa.</description><subject>Allium</subject><subject>Amaryllidaceae - anatomy & histology</subject><subject>Amaryllidaceae - classification</subject><subject>Anatomy</subject><subject>Araceae - anatomy & histology</subject><subject>Araceae - classification</subject><subject>Asparagaceae - anatomy & histology</subject><subject>Asparagaceae - classification</subject><subject>Canna indica</subject><subject>Cell size</subject><subject>Climatic zones</subject><subject>Ecosystem</subject><subject>Epidermal Cells - ultrastructure</subject><subject>light and scanning electron microscopy</subject><subject>Light microscopy</subject><subject>Liliaceae - anatomy & histology</subject><subject>Liliaceae - classification</subject><subject>Microscopy, Electron, Scanning</subject><subject>monocot</subject><subject>Morphology</subject><subject>Pakistan</subject><subject>palyno‐anatomical features</subject><subject>Plant Epidermis - ultrastructure</subject><subject>Pollen</subject><subject>Pollen - ultrastructure</subject><subject>Scanning electron microscopy</subject><subject>Stomata</subject><subject>Taxa</subject><subject>Taxonomic significance</subject><subject>Taxonomy</subject><issn>1059-910X</issn><issn>1097-0029</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kU1u2zAQhYkiQe2k3fQABYFsggBKSVEUxWVguPlBgnbhAt0JY4pKaVCkK1JwvcsRcsacpJSdZJFFVhw-fPMwMw-hL5ScU0LybyvdxfOcUSE_oCklUmRJlQdjzWUmKfk9QUchrAihlNPiI5owwgWnlZyizU-wW-efHh7BQfSdUWBxiENjdMC-xZ13XvmII_wDDK7BJobxk-TEYtOtbWqJxruAh2DcPbbm_k_coUGBc6OkrVax9w6nlt4H5dfbT-iwBRv05-f3GP36Pl_MrrLbH5fXs4vbTDEuZFaWhSoUqYCX0FSlbgoQpBKgqioXOVSQtiZS82K5LBpggnPdkmXLgDSaS0XYMTrd-657_3fQIdadCUpbC077IdQ55VJWOWV5Qk_eoCs_9C5NlyhZMlYKyhN1tqfGTUKv23rdmw76bU1JPcZRj3HUuzgS_PXZclh2unlFX-6fALoHNsbq7TtW9c38brE3_Q85NZhK</recordid><startdate>201904</startdate><enddate>201904</enddate><creator>Bahadur, Saraj</creator><creator>Ahmad, Mushtaq</creator><creator>Zafar, Muhammad</creator><creator>Sultana, Shazia</creator><creator>Begum, Najla</creator><creator>Ashfaq, Shomaila</creator><creator>Gul, Saba</creator><creator>Khan, Muhammad Saleem</creator><creator>Shah, Syed Nasar</creator><creator>Ullah, Fazal</creator><creator>Saqib, Saddam</creator><creator>Ayaz, Asma</creator><general>John Wiley & Sons, Inc</general><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QP</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7SS</scope><scope>7TA</scope><scope>7TB</scope><scope>7U5</scope><scope>7U7</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>JG9</scope><scope>JQ2</scope><scope>K9.</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-5496-7861</orcidid><orcidid>https://orcid.org/0000-0002-9002-6110</orcidid><orcidid>https://orcid.org/0000-0002-1240-8949</orcidid><orcidid>https://orcid.org/0000-0003-2971-2848</orcidid><orcidid>https://orcid.org/0000-0003-2002-3907</orcidid><orcidid>https://orcid.org/0000-0001-5856-3189</orcidid><orcidid>https://orcid.org/0000-0002-3378-9509</orcidid></search><sort><creationdate>201904</creationdate><title>Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy</title><author>Bahadur, Saraj ; Ahmad, Mushtaq ; Zafar, Muhammad ; Sultana, Shazia ; Begum, Najla ; Ashfaq, Shomaila ; Gul, Saba ; Khan, Muhammad Saleem ; Shah, Syed Nasar ; Ullah, Fazal ; Saqib, Saddam ; Ayaz, Asma</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3579-664c4c08a56ad86ed4a7087ac88272a8a31709e54bb4da3755ef0bf3a0de59c03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Allium</topic><topic>Amaryllidaceae - anatomy & histology</topic><topic>Amaryllidaceae - classification</topic><topic>Anatomy</topic><topic>Araceae - anatomy & histology</topic><topic>Araceae - classification</topic><topic>Asparagaceae - anatomy & histology</topic><topic>Asparagaceae - classification</topic><topic>Canna indica</topic><topic>Cell size</topic><topic>Climatic zones</topic><topic>Ecosystem</topic><topic>Epidermal Cells - ultrastructure</topic><topic>light and scanning electron microscopy</topic><topic>Light microscopy</topic><topic>Liliaceae - anatomy & histology</topic><topic>Liliaceae - classification</topic><topic>Microscopy, Electron, Scanning</topic><topic>monocot</topic><topic>Morphology</topic><topic>Pakistan</topic><topic>palyno‐anatomical features</topic><topic>Plant Epidermis - ultrastructure</topic><topic>Pollen</topic><topic>Pollen - ultrastructure</topic><topic>Scanning electron microscopy</topic><topic>Stomata</topic><topic>Taxa</topic><topic>Taxonomic significance</topic><topic>Taxonomy</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bahadur, Saraj</creatorcontrib><creatorcontrib>Ahmad, Mushtaq</creatorcontrib><creatorcontrib>Zafar, Muhammad</creatorcontrib><creatorcontrib>Sultana, Shazia</creatorcontrib><creatorcontrib>Begum, Najla</creatorcontrib><creatorcontrib>Ashfaq, Shomaila</creatorcontrib><creatorcontrib>Gul, Saba</creatorcontrib><creatorcontrib>Khan, Muhammad Saleem</creatorcontrib><creatorcontrib>Shah, Syed Nasar</creatorcontrib><creatorcontrib>Ullah, Fazal</creatorcontrib><creatorcontrib>Saqib, Saddam</creatorcontrib><creatorcontrib>Ayaz, Asma</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Microscopy research and technique</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bahadur, Saraj</au><au>Ahmad, Mushtaq</au><au>Zafar, Muhammad</au><au>Sultana, Shazia</au><au>Begum, Najla</au><au>Ashfaq, Shomaila</au><au>Gul, Saba</au><au>Khan, Muhammad Saleem</au><au>Shah, Syed Nasar</au><au>Ullah, Fazal</au><au>Saqib, Saddam</au><au>Ayaz, Asma</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy</atitle><jtitle>Microscopy research and technique</jtitle><addtitle>Microsc Res Tech</addtitle><date>2019-04</date><risdate>2019</risdate><volume>82</volume><issue>4</issue><spage>373</spage><epage>393</epage><pages>373-393</pages><issn>1059-910X</issn><eissn>1097-0029</eissn><abstract>Palyno‐anatomical study of monocots taxa using Light and Scanning Electron Microscopy (SEM) was first time conducted with a view to evaluating their taxonomic significance. Studied plants were collected from different eco‐climatic zones of Pakistan ranges from tropical, sub‐tropical, and moist habitats. The aim of this study is to use palyno‐anatomical features for the correct identification, systematic comparison, and investigation to elucidate the taxonomic significance of these features, which are useful to taxonomists for identifying monocot taxa. A signification variation was observed in quantitative and qualitative characters by using the standard protocol of light microscopy (LM) and SEM. Epidermal cell length varied from maximum in Allium griffthianum (480 ± 35.9) μm at the adaxial surface to minimum in Canna indica (33.6 ± 8.53) μm on abaxial surface. Maximum exine thickness was observed in Canna indica (4.46) μm and minimum in Allium grifthianum (0.8) μm. Variation was observed in shape and exine ornamentation of the pollen, shape of the epidermal cell, number, size, and type of stomata, guard cell shape, and anticlinal wall pattern. Based on these palyno‐anatomical features a taxonomic key was developed, which help in the discrimination of studied taxa. In conclusion, LM and SEM pollen and epidermal morphology is explanatory, significant, and can be of special interest for the plant taxonomist in the correct identification of monocots taxa.
The SEM and LM method use as a tool in the identification of monocot taxa.
Palyno‐anatomical features were first time documented for its taxonomic significance.
Based on these features, a taxonomic key was developed for the correct identification and delimitation of the monocot taxa.</abstract><cop>Hoboken, USA</cop><pub>John Wiley & Sons, Inc</pub><pmid>30575189</pmid><doi>10.1002/jemt.23179</doi><tpages>21</tpages><orcidid>https://orcid.org/0000-0002-5496-7861</orcidid><orcidid>https://orcid.org/0000-0002-9002-6110</orcidid><orcidid>https://orcid.org/0000-0002-1240-8949</orcidid><orcidid>https://orcid.org/0000-0003-2971-2848</orcidid><orcidid>https://orcid.org/0000-0003-2002-3907</orcidid><orcidid>https://orcid.org/0000-0001-5856-3189</orcidid><orcidid>https://orcid.org/0000-0002-3378-9509</orcidid></addata></record> |
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subjects | Allium Amaryllidaceae - anatomy & histology Amaryllidaceae - classification Anatomy Araceae - anatomy & histology Araceae - classification Asparagaceae - anatomy & histology Asparagaceae - classification Canna indica Cell size Climatic zones Ecosystem Epidermal Cells - ultrastructure light and scanning electron microscopy Light microscopy Liliaceae - anatomy & histology Liliaceae - classification Microscopy, Electron, Scanning monocot Morphology Pakistan palyno‐anatomical features Plant Epidermis - ultrastructure Pollen Pollen - ultrastructure Scanning electron microscopy Stomata Taxa Taxonomic significance Taxonomy |
title | Palyno‐anatomical studies of monocot taxa and its taxonomic implications using light and scanning electron microscopy |
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