Characterization and visualization of the liposecretion process taking place during ceiling culture of human mature adipocytes

Objective To investigate and further characterize the process of mature adipocyte dedifferentiation. Our hypothesis was that dedifferentiation does not involve mitosis but rather a phenomenon of liposecretion. Methods Mature adipocytes were isolated by collagenase digestion of human adipose tissue s...

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Veröffentlicht in:	Journal of cellular physiology 2019-07, Vol.234 (7), p.10270-10280
Hauptverfasser:	Côté, Julie Anne, Gauthier, Marie‐Frédérique, Ostinelli, Giada, Brochu, Dannick, Bellmann, Kerstin, Marette, André, Julien, François, Lebel, Stéfane, Tchernof, André
Format:	Artikel
Sprache:	eng
Schlagworte:	adipocyte Adipocytes Adipocytes - cytology Adipocytes - metabolism Adipose tissue Baculovirus ceiling culture Cell culture Cell Culture Techniques - methods Cell cycle Cell Dedifferentiation - physiology Cell division Cells, Cultured Collagen Collagenase Cyclin B1 Cytosine dedifferentiation Droplets Female Fibroblasts human Humans Lipid Droplets - metabolism Lipids liposecretion Male Microscopy Mitosis Phosphorylation Secretion Vincristine
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