HPLC profiling of Mimosa pudica polyphenols and their non-invasive biophysical investigations for anti-dermatoheliotic and skin reinstating potential

Extraction and fractionation of Mimosa pudica seeds were carried out using solvents of increasing polarity. Hydro-alcoholic crude extract and three fractions (HAMP, NHMP, CLMP, and NBMP) were subjected to phytochemical analysis to estimate total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant and anti-tyrosinase activity along with HPLC profiling for their polyphenols. Depending upon these results, HAMP found to be appropriate for our in-vivo study made to formulate topical emulgel formulation after evaluating its safety and cytocompatibility. The formulation was then subjected to non-invasive in-vivo evaluation on human volunteers after performing its skin irritation potential by patch test. [Display omitted] Plant-derived polyphenols are known to have promising biological activities including antioxidant and antityrosinase and may be a potential candidate for anti-dermatoheliotic remedy. The present study was performed to investigate the polyphenolic contents of Mimosa pudica (MP) seed extract and its anti-dermatoheliotic potential using non-invasive biophysical techniques after developing a stable topical emulgel formulation. Moreover, its in-vitro cytotoxicity was also evaluated using normal Human keratinocytes (HaCat cells) to rule out any cellular incompatibility. The results revealed that MP seed extract, constituted with a number of polyphenolic compounds, has very good antioxidant and anti-tyrosinase potential. There were significant positive effects (p ≤ 0.05) invoked by its topical emulgel formulation on various dermatoheliotic associated skin parameters like erythema, melanin, elasticity, hydration, and sebum as compared to placebo. In the meantime, it was also found to be biocompatible and did not show any effect on HaCat cell viability and structure. In conclusion, the topical emulgel preparation loaded with MP seed extract could be a great strategy to deal with dematoheliosis.