Modulation of ABA responses by the protein kinase WNK8
Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2‐type protein kinases. Other signaling modules might con...
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| Veröffentlicht in: | FEBS letters 2019-02, Vol.593 (3), p.339-351 |
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| creator | Waadt, Rainer Jawurek, Esther Hashimoto, Kenji Li, Yan Scholz, Martin Krebs, Melanie Czap, Gereon Hong‐Hermesdorf, Anne Hippler, Michael Grill, Erwin Kudla, Jörg Schumacher, Karin |
| description | Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2‐type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8‐ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca‐1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA‐induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling. |
| doi_str_mv | 10.1002/1873-3468.13315 |
| format | Article |
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ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2‐type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8‐ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca‐1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA‐induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1002/1873-3468.13315</identifier><identifier>PMID: 30556127</identifier><language>eng</language><publisher>England</publisher><subject>abscisic acid ; Abscisic Acid - genetics ; Abscisic Acid - metabolism ; Alleles ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Membrane Transport Proteins - genetics ; Membrane Transport Proteins - metabolism ; Mutation ; Nicotiana - genetics ; Nicotiana - metabolism ; Phosphoprotein Phosphatases - genetics ; Phosphoprotein Phosphatases - metabolism ; protein kinase WNK8 ; protein phosphatase PP2CA ; Protein Serine-Threonine Kinases - genetics ; Protein Serine-Threonine Kinases - metabolism ; Protoplasts - enzymology ; seed germination ; Signal Transduction - physiology</subject><ispartof>FEBS letters, 2019-02, Vol.593 (3), p.339-351</ispartof><rights>2018 Federation of European Biochemical Societies</rights><rights>2018 Federation of European Biochemical Societies.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3855-af7b61e092ddd74f5f6329d25759aad9fb3db9947572fc0e2bcc21125fab712d3</citedby><cites>FETCH-LOGICAL-c3855-af7b61e092ddd74f5f6329d25759aad9fb3db9947572fc0e2bcc21125fab712d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2F1873-3468.13315$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2F1873-3468.13315$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30556127$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Waadt, Rainer</creatorcontrib><creatorcontrib>Jawurek, Esther</creatorcontrib><creatorcontrib>Hashimoto, Kenji</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Scholz, Martin</creatorcontrib><creatorcontrib>Krebs, Melanie</creatorcontrib><creatorcontrib>Czap, Gereon</creatorcontrib><creatorcontrib>Hong‐Hermesdorf, Anne</creatorcontrib><creatorcontrib>Hippler, Michael</creatorcontrib><creatorcontrib>Grill, Erwin</creatorcontrib><creatorcontrib>Kudla, Jörg</creatorcontrib><creatorcontrib>Schumacher, Karin</creatorcontrib><title>Modulation of ABA responses by the protein kinase WNK8</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2‐type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8‐ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca‐1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA‐induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.</description><subject>abscisic acid</subject><subject>Abscisic Acid - genetics</subject><subject>Abscisic Acid - metabolism</subject><subject>Alleles</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Membrane Transport Proteins - genetics</subject><subject>Membrane Transport Proteins - metabolism</subject><subject>Mutation</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana - metabolism</subject><subject>Phosphoprotein Phosphatases - genetics</subject><subject>Phosphoprotein Phosphatases - metabolism</subject><subject>protein kinase WNK8</subject><subject>protein phosphatase PP2CA</subject><subject>Protein Serine-Threonine Kinases - genetics</subject><subject>Protein Serine-Threonine Kinases - metabolism</subject><subject>Protoplasts - enzymology</subject><subject>seed germination</subject><subject>Signal Transduction - physiology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9PwjAYQBujEUTP3kyPXgb9sbbrEQioEfWi8di0axunY8V1C-G_dzjk6qn5mve9fHkAXGM0xgiRCc4ETWjKszGmFLMTMDz-nIIhQjhNmJB0AC5i_ETdnGF5DgYUMcYxEUPAn4JtS90UoYLBw-lsCmsXN6GKLkKzg82Hg5s6NK6o4FdR6ejg-_NjdgnOvC6juzq8I_C2XLzO75PVy93DfLpKcpoxlmgvDMcOSWKtFalnnlMiLWGCSa2t9IZaI2UqmCA-R46YPCcYE-a1EZhYOgK3vbe74bt1sVHrIuauLHXlQhsVwUxwlkqeduikR_M6xFg7rzZ1sdb1TmGk9rHUPo3ap1G_sbqNm4O8NWtnj_xfnQ7gPbAtSrf7z6eWixnpzT-_1XH6</recordid><startdate>201902</startdate><enddate>201902</enddate><creator>Waadt, Rainer</creator><creator>Jawurek, Esther</creator><creator>Hashimoto, Kenji</creator><creator>Li, Yan</creator><creator>Scholz, Martin</creator><creator>Krebs, Melanie</creator><creator>Czap, Gereon</creator><creator>Hong‐Hermesdorf, Anne</creator><creator>Hippler, Michael</creator><creator>Grill, Erwin</creator><creator>Kudla, Jörg</creator><creator>Schumacher, Karin</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201902</creationdate><title>Modulation of ABA responses by the protein kinase WNK8</title><author>Waadt, Rainer ; Jawurek, Esther ; Hashimoto, Kenji ; Li, Yan ; Scholz, Martin ; Krebs, Melanie ; Czap, Gereon ; Hong‐Hermesdorf, Anne ; Hippler, Michael ; Grill, Erwin ; Kudla, Jörg ; Schumacher, Karin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3855-af7b61e092ddd74f5f6329d25759aad9fb3db9947572fc0e2bcc21125fab712d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>abscisic acid</topic><topic>Abscisic Acid - genetics</topic><topic>Abscisic Acid - metabolism</topic><topic>Alleles</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Membrane Transport Proteins - genetics</topic><topic>Membrane Transport Proteins - metabolism</topic><topic>Mutation</topic><topic>Nicotiana - genetics</topic><topic>Nicotiana - metabolism</topic><topic>Phosphoprotein Phosphatases - genetics</topic><topic>Phosphoprotein Phosphatases - metabolism</topic><topic>protein kinase WNK8</topic><topic>protein phosphatase PP2CA</topic><topic>Protein Serine-Threonine Kinases - genetics</topic><topic>Protein Serine-Threonine Kinases - metabolism</topic><topic>Protoplasts - enzymology</topic><topic>seed germination</topic><topic>Signal Transduction - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Waadt, Rainer</creatorcontrib><creatorcontrib>Jawurek, Esther</creatorcontrib><creatorcontrib>Hashimoto, Kenji</creatorcontrib><creatorcontrib>Li, Yan</creatorcontrib><creatorcontrib>Scholz, Martin</creatorcontrib><creatorcontrib>Krebs, Melanie</creatorcontrib><creatorcontrib>Czap, Gereon</creatorcontrib><creatorcontrib>Hong‐Hermesdorf, Anne</creatorcontrib><creatorcontrib>Hippler, Michael</creatorcontrib><creatorcontrib>Grill, Erwin</creatorcontrib><creatorcontrib>Kudla, Jörg</creatorcontrib><creatorcontrib>Schumacher, Karin</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Waadt, Rainer</au><au>Jawurek, Esther</au><au>Hashimoto, Kenji</au><au>Li, Yan</au><au>Scholz, Martin</au><au>Krebs, Melanie</au><au>Czap, Gereon</au><au>Hong‐Hermesdorf, Anne</au><au>Hippler, Michael</au><au>Grill, Erwin</au><au>Kudla, Jörg</au><au>Schumacher, Karin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Modulation of ABA responses by the protein kinase WNK8</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2019-02</date><risdate>2019</risdate><volume>593</volume><issue>3</issue><spage>339</spage><epage>351</epage><pages>339-351</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Abscisic acid (ABA) regulates growth and developmental processes in response to limiting water conditions. ABA functions through a core signaling pathway consisting of PYR1/PYL/RCAR ABA receptors, type 2C protein phosphatases (PP2Cs), and SnRK2‐type protein kinases. Other signaling modules might converge with ABA signals through the modulation of core ABA signaling components. We have investigated the role of the protein kinase WNK8 in ABA signaling. WNK8 interacted with PP2CA and PYR1, phosphorylated PYR1 in vitro, and was dephosphorylated by PP2CA. A hypermorphic wnk8‐ct Arabidopsis mutant allele suppressed ABA and glucose hypersensitivities of pp2ca‐1 mutants during young seedling development, and WNK8 expression in protoplasts suppressed ABA‐induced reporter gene expression. We conclude that WNK8 functions as a negative modulator of ABA signaling.</abstract><cop>England</cop><pmid>30556127</pmid><doi>10.1002/1873-3468.13315</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via Wiley Online Library; Wiley Free Content; Alma/SFX Local Collection |
| subjects | abscisic acid Abscisic Acid - genetics Abscisic Acid - metabolism Alleles Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Membrane Transport Proteins - genetics Membrane Transport Proteins - metabolism Mutation Nicotiana - genetics Nicotiana - metabolism Phosphoprotein Phosphatases - genetics Phosphoprotein Phosphatases - metabolism protein kinase WNK8 protein phosphatase PP2CA Protein Serine-Threonine Kinases - genetics Protein Serine-Threonine Kinases - metabolism Protoplasts - enzymology seed germination Signal Transduction - physiology |
| title | Modulation of ABA responses by the protein kinase WNK8 |
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