Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities
Lignosus rhinocerotis (Cooke) Ryvarden (Tiger milk mushroom) is traditionally used to treat inflammation triggered symptoms and illnesses such as cough, fever and asthma. The present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fraction...
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| Veröffentlicht in: | Natural product communications 2016-10, Vol.11 (10), p.1485-1490 |
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| creator | Nallathamby, Neeranjini Serm, Lee Guan Raman, Jegadeesh Malek, Sri Nurestri Abd Vidyadaran, Sharmili Naidu, Murali Kuppusamy, Umah Rani Sabaratnam, Vikineswary |
| description | Lignosus rhinocerotis (Cooke) Ryvarden (Tiger milk mushroom) is traditionally used to treat inflammation triggered symptoms and illnesses such as cough, fever and asthma. The present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fractions of sclerotia powder of L. rhinocerotis on brain microglial (BV2) cells. The ethyl acetate fraction had a total phenolic content of 0.30 ± 0.11 mg GAE/g. This fraction had ferric reducing capacity of 61.8 ± 1.8 mg FSE/g, ABTS•+ scavenging activity of 36.8 ± 1.8 mg TE/g and DPPH free radical scavenging activity of 21.8% ± 0.7. At doses ranging from 0.1 μg/mL – 100 μg/mL, the extract and fractions were not cytotoxic to BV2 cells. At 100 μg/mL, the crude hydroethanolic extract and the ethyl acetate fraction elicited the highest nitric oxide reduction activities of 68.7% and 58.2%, respectively. Linoleic and oleic acids were the major lipid constituents in the ethyl acetate fraction based on FID and GC-MS analysis. Linoleic acid reduced nitric oxide production and down regulated the expression of neuroinflammatory iNOS and COX2 genes in BV2 cells. |
| doi_str_mv | 10.1177/1934578X1601101016 |
| format | Article |
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The present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fractions of sclerotia powder of L. rhinocerotis on brain microglial (BV2) cells. The ethyl acetate fraction had a total phenolic content of 0.30 ± 0.11 mg GAE/g. This fraction had ferric reducing capacity of 61.8 ± 1.8 mg FSE/g, ABTS•+ scavenging activity of 36.8 ± 1.8 mg TE/g and DPPH free radical scavenging activity of 21.8% ± 0.7. At doses ranging from 0.1 μg/mL – 100 μg/mL, the extract and fractions were not cytotoxic to BV2 cells. At 100 μg/mL, the crude hydroethanolic extract and the ethyl acetate fraction elicited the highest nitric oxide reduction activities of 68.7% and 58.2%, respectively. Linoleic and oleic acids were the major lipid constituents in the ethyl acetate fraction based on FID and GC-MS analysis. Linoleic acid reduced nitric oxide production and down regulated the expression of neuroinflammatory iNOS and COX2 genes in BV2 cells.</description><identifier>ISSN: 1934-578X</identifier><identifier>EISSN: 1555-9475</identifier><identifier>DOI: 10.1177/1934578X1601101016</identifier><identifier>PMID: 30549604</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Anti-Inflammatory Agents - chemistry ; Anti-Inflammatory Agents - pharmacology ; Antineoplastic Agents - pharmacology ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Brain Chemistry - drug effects ; Cell Line ; Free Radical Scavengers - pharmacology ; Gas Chromatography-Mass Spectrometry ; Humans ; Linoleic Acid - chemistry ; Linoleic Acid - pharmacology ; Lipids - chemistry ; Lipids - pharmacology ; Microglia - drug effects ; Neuritis - drug therapy ; Nitric Oxide - antagonists & inhibitors ; Nitric Oxide - biosynthesis ; Oleic Acid - chemistry ; Oleic Acid - pharmacology ; Polyporaceae - chemistry</subject><ispartof>Natural product communications, 2016-10, Vol.11 (10), p.1485-1490</ispartof><rights>2016 SAGE Publications Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c343t-9953cd1172f9e2d464066671f696de544b4ed109bb092f9eedf22afec70ec3b23</citedby><cites>FETCH-LOGICAL-c343t-9953cd1172f9e2d464066671f696de544b4ed109bb092f9eedf22afec70ec3b23</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1934578X1601101016$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1934578X1601101016$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21945,27830,27901,27902,44921,45309</link.rule.ids><linktorsrc>$$Uhttps://journals.sagepub.com/doi/full/10.1177/1934578X1601101016?utm_source=summon&utm_medium=discovery-provider$$EView_record_in_SAGE_Publications$$FView_record_in_$$GSAGE_Publications</linktorsrc><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30549604$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nallathamby, Neeranjini</creatorcontrib><creatorcontrib>Serm, Lee Guan</creatorcontrib><creatorcontrib>Raman, Jegadeesh</creatorcontrib><creatorcontrib>Malek, Sri Nurestri Abd</creatorcontrib><creatorcontrib>Vidyadaran, Sharmili</creatorcontrib><creatorcontrib>Naidu, Murali</creatorcontrib><creatorcontrib>Kuppusamy, Umah Rani</creatorcontrib><creatorcontrib>Sabaratnam, Vikineswary</creatorcontrib><title>Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities</title><title>Natural product communications</title><addtitle>Nat Prod Commun</addtitle><description>Lignosus rhinocerotis (Cooke) Ryvarden (Tiger milk mushroom) is traditionally used to treat inflammation triggered symptoms and illnesses such as cough, fever and asthma. The present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fractions of sclerotia powder of L. rhinocerotis on brain microglial (BV2) cells. The ethyl acetate fraction had a total phenolic content of 0.30 ± 0.11 mg GAE/g. This fraction had ferric reducing capacity of 61.8 ± 1.8 mg FSE/g, ABTS•+ scavenging activity of 36.8 ± 1.8 mg TE/g and DPPH free radical scavenging activity of 21.8% ± 0.7. At doses ranging from 0.1 μg/mL – 100 μg/mL, the extract and fractions were not cytotoxic to BV2 cells. At 100 μg/mL, the crude hydroethanolic extract and the ethyl acetate fraction elicited the highest nitric oxide reduction activities of 68.7% and 58.2%, respectively. Linoleic and oleic acids were the major lipid constituents in the ethyl acetate fraction based on FID and GC-MS analysis. Linoleic acid reduced nitric oxide production and down regulated the expression of neuroinflammatory iNOS and COX2 genes in BV2 cells.</description><subject>Anti-Inflammatory Agents - chemistry</subject><subject>Anti-Inflammatory Agents - pharmacology</subject><subject>Antineoplastic Agents - pharmacology</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Brain Chemistry - drug effects</subject><subject>Cell Line</subject><subject>Free Radical Scavengers - pharmacology</subject><subject>Gas Chromatography-Mass Spectrometry</subject><subject>Humans</subject><subject>Linoleic Acid - chemistry</subject><subject>Linoleic Acid - pharmacology</subject><subject>Lipids - chemistry</subject><subject>Lipids - pharmacology</subject><subject>Microglia - drug effects</subject><subject>Neuritis - drug therapy</subject><subject>Nitric Oxide - antagonists & inhibitors</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Oleic Acid - chemistry</subject><subject>Oleic Acid - pharmacology</subject><subject>Polyporaceae - chemistry</subject><issn>1934-578X</issn><issn>1555-9475</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u3CAQgFHUKonSvEAOFcdenIAN2BxXq22z0ko5NJFyszAMKZENW8BR8xJ95rDxtpdKhQM_882HmEHoipJrStv2hsqG8bZ7pIJQSsoUJ-iccs4ryVr-oewLUB2IM3SZ0jMpo-sYYfIUnTWEMykIO0e_twZ8dtZplV3wWHmDnccvLseANy9qnJf7YPHO7Z1J2MYw4e96hBiyU0vgyYc0Jxx_OB_0e6BwIeJVUYdfziif382Hc-VhjsF5O6ppUjnEV7zS2ZUXHaRP6KNVY4LL43qBHr5u7te31e7u23a92lW6YU2upOSNNqUOtZVQGyYYEUK01AopDHDGBgaGEjkMRB4QMLaulQXdEtDNUDcX6Mvi3cfwc4aU-8klDeOoPIQ59TXlreBN1_GC1guqY0gpgu330U0qvvaU9IdW9P-2oiR9PvrnYQLzN-VP4QtwswBJPUH_HOboy3__p3wDugWUsg</recordid><startdate>201610</startdate><enddate>201610</enddate><creator>Nallathamby, Neeranjini</creator><creator>Serm, Lee Guan</creator><creator>Raman, Jegadeesh</creator><creator>Malek, Sri Nurestri Abd</creator><creator>Vidyadaran, Sharmili</creator><creator>Naidu, Murali</creator><creator>Kuppusamy, Umah Rani</creator><creator>Sabaratnam, Vikineswary</creator><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201610</creationdate><title>Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities</title><author>Nallathamby, Neeranjini ; Serm, Lee Guan ; Raman, Jegadeesh ; Malek, Sri Nurestri Abd ; Vidyadaran, Sharmili ; Naidu, Murali ; Kuppusamy, Umah Rani ; Sabaratnam, Vikineswary</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c343t-9953cd1172f9e2d464066671f696de544b4ed109bb092f9eedf22afec70ec3b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Inflammatory Agents - chemistry</topic><topic>Anti-Inflammatory Agents - pharmacology</topic><topic>Antineoplastic Agents - pharmacology</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - pharmacology</topic><topic>Brain Chemistry - drug effects</topic><topic>Cell Line</topic><topic>Free Radical Scavengers - pharmacology</topic><topic>Gas Chromatography-Mass Spectrometry</topic><topic>Humans</topic><topic>Linoleic Acid - chemistry</topic><topic>Linoleic Acid - pharmacology</topic><topic>Lipids - chemistry</topic><topic>Lipids - pharmacology</topic><topic>Microglia - drug effects</topic><topic>Neuritis - drug therapy</topic><topic>Nitric Oxide - antagonists & inhibitors</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Oleic Acid - chemistry</topic><topic>Oleic Acid - pharmacology</topic><topic>Polyporaceae - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nallathamby, Neeranjini</creatorcontrib><creatorcontrib>Serm, Lee Guan</creatorcontrib><creatorcontrib>Raman, Jegadeesh</creatorcontrib><creatorcontrib>Malek, Sri Nurestri Abd</creatorcontrib><creatorcontrib>Vidyadaran, Sharmili</creatorcontrib><creatorcontrib>Naidu, Murali</creatorcontrib><creatorcontrib>Kuppusamy, Umah Rani</creatorcontrib><creatorcontrib>Sabaratnam, Vikineswary</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Natural product communications</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>Nallathamby, Neeranjini</au><au>Serm, Lee Guan</au><au>Raman, Jegadeesh</au><au>Malek, Sri Nurestri Abd</au><au>Vidyadaran, Sharmili</au><au>Naidu, Murali</au><au>Kuppusamy, Umah Rani</au><au>Sabaratnam, Vikineswary</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities</atitle><jtitle>Natural product communications</jtitle><addtitle>Nat Prod Commun</addtitle><date>2016-10</date><risdate>2016</risdate><volume>11</volume><issue>10</issue><spage>1485</spage><epage>1490</epage><pages>1485-1490</pages><issn>1934-578X</issn><eissn>1555-9475</eissn><abstract>Lignosus rhinocerotis (Cooke) Ryvarden (Tiger milk mushroom) is traditionally used to treat inflammation triggered symptoms and illnesses such as cough, fever and asthma. The present study evaluated the in vitro antioxidant, cytotoxic and anti-neuroinflammatory activities of the extract and fractions of sclerotia powder of L. rhinocerotis on brain microglial (BV2) cells. The ethyl acetate fraction had a total phenolic content of 0.30 ± 0.11 mg GAE/g. This fraction had ferric reducing capacity of 61.8 ± 1.8 mg FSE/g, ABTS•+ scavenging activity of 36.8 ± 1.8 mg TE/g and DPPH free radical scavenging activity of 21.8% ± 0.7. At doses ranging from 0.1 μg/mL – 100 μg/mL, the extract and fractions were not cytotoxic to BV2 cells. At 100 μg/mL, the crude hydroethanolic extract and the ethyl acetate fraction elicited the highest nitric oxide reduction activities of 68.7% and 58.2%, respectively. Linoleic and oleic acids were the major lipid constituents in the ethyl acetate fraction based on FID and GC-MS analysis. Linoleic acid reduced nitric oxide production and down regulated the expression of neuroinflammatory iNOS and COX2 genes in BV2 cells.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>30549604</pmid><doi>10.1177/1934578X1601101016</doi><tpages>6</tpages></addata></record> |
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| source | Sage Journals GOLD Open Access 2024 |
| subjects | Anti-Inflammatory Agents - chemistry Anti-Inflammatory Agents - pharmacology Antineoplastic Agents - pharmacology Antioxidants - chemistry Antioxidants - pharmacology Brain Chemistry - drug effects Cell Line Free Radical Scavengers - pharmacology Gas Chromatography-Mass Spectrometry Humans Linoleic Acid - chemistry Linoleic Acid - pharmacology Lipids - chemistry Lipids - pharmacology Microglia - drug effects Neuritis - drug therapy Nitric Oxide - antagonists & inhibitors Nitric Oxide - biosynthesis Oleic Acid - chemistry Oleic Acid - pharmacology Polyporaceae - chemistry |
| title | Identification and in vitro Evaluation of Lipids from Sclerotia of Lignosus rhinocerotis for Antioxidant and Anti-neuroinflammatory Activities |
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