Escherichia coli lipopolysaccharide induces alveolar epithelial cell stiffening
Application of lipopolysaccharide (LPS) is a widely employed model to mimic acute respiratory distress syndrome (ARDS). Available data regarding LPS-induced biomechanical changes on pulmonary epithelial cells are limited only to P. aeruginosa LPS. Considering that LPS from different bacteria could p...
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| Veröffentlicht in: | Journal of biomechanics 2019-01, Vol.83, p.315-318 |
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| creator | Oliveira, Vinícius Rosa Uriarte, Juan José Falcones, Bryan Zin, Walter Araujo Navajas, Daniel Farré, Ramon Almendros, Isaac |
| description | Application of lipopolysaccharide (LPS) is a widely employed model to mimic acute respiratory distress syndrome (ARDS). Available data regarding LPS-induced biomechanical changes on pulmonary epithelial cells are limited only to P. aeruginosa LPS. Considering that LPS from different bacteria could promote a specific mechanical response in epithelial cells, we aim to assess the effect of E. coli LPS, widely employed as a model of ARDS, in the biomechanics of alveolar epithelial cells.
Young’s modulus (E) of alveolar epithelial cells (A549) was measured by atomic force microscopy every 5 min throughout 60 min of experiment after treatment with LPS from E. coli (100 μg/mL). The percentage of cells presenting actin stress fibers (F-actin staining) was also evaluated. Control cells were treated with culture medium and the values obtained were compared with LPS-treated cells for each time-point.
Application of LPS induced significant increase in E after 20 min (77%) till 60 min (104%) in comparison to controls. Increase in lung epithelial cell stiffness induced by LPS was associated with a higher number of cells presenting cytoskeletal remodeling.
The observed effects of E. coli LPS on alveolar epithelial cells suggest that this widely-used LPS is able to promote a quick formation of actin stress fibers and stiffening cells, thereby facilitating the disruption of the pulmonary epithelial barrier. |
| doi_str_mv | 10.1016/j.jbiomech.2018.11.034 |
| format | Article |
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Young’s modulus (E) of alveolar epithelial cells (A549) was measured by atomic force microscopy every 5 min throughout 60 min of experiment after treatment with LPS from E. coli (100 μg/mL). The percentage of cells presenting actin stress fibers (F-actin staining) was also evaluated. Control cells were treated with culture medium and the values obtained were compared with LPS-treated cells for each time-point.
Application of LPS induced significant increase in E after 20 min (77%) till 60 min (104%) in comparison to controls. Increase in lung epithelial cell stiffness induced by LPS was associated with a higher number of cells presenting cytoskeletal remodeling.
The observed effects of E. coli LPS on alveolar epithelial cells suggest that this widely-used LPS is able to promote a quick formation of actin stress fibers and stiffening cells, thereby facilitating the disruption of the pulmonary epithelial barrier.</description><identifier>ISSN: 0021-9290</identifier><identifier>EISSN: 1873-2380</identifier><identifier>DOI: 10.1016/j.jbiomech.2018.11.034</identifier><identifier>PMID: 30527389</identifier><language>eng</language><publisher>United States: Elsevier Ltd</publisher><subject>Actin ; Acute respiratory distress syndrome model ; Alveolar epithelium ; Alveoli ; Atomic force microscopy ; Biomechanics ; Cell culture ; Cytoskeleton ; Disruption ; E coli ; Epithelial cells ; Escherichia coli ; Inflammation ; Lipopolysaccharide ; Lipopolysaccharides ; Lungs ; Mechanical analysis ; Mechanical properties ; Modulus of elasticity ; Pseudomonas aeruginosa ; Respiratory distress syndrome ; Stiffening ; Stiffness ; Variance analysis</subject><ispartof>Journal of biomechanics, 2019-01, Vol.83, p.315-318</ispartof><rights>2018 Elsevier Ltd</rights><rights>Copyright © 2018 Elsevier Ltd. All rights reserved.</rights><rights>Copyright Elsevier Limited Jan 23, 2019</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-25a8bf9302a782c0531cb8f20d485ace60ef2248e12aa6ebc752fe152770d1fc3</citedby><cites>FETCH-LOGICAL-c433t-25a8bf9302a782c0531cb8f20d485ace60ef2248e12aa6ebc752fe152770d1fc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/2164128649?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000,64390,64392,64394,72474</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30527389$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oliveira, Vinícius Rosa</creatorcontrib><creatorcontrib>Uriarte, Juan José</creatorcontrib><creatorcontrib>Falcones, Bryan</creatorcontrib><creatorcontrib>Zin, Walter Araujo</creatorcontrib><creatorcontrib>Navajas, Daniel</creatorcontrib><creatorcontrib>Farré, Ramon</creatorcontrib><creatorcontrib>Almendros, Isaac</creatorcontrib><title>Escherichia coli lipopolysaccharide induces alveolar epithelial cell stiffening</title><title>Journal of biomechanics</title><addtitle>J Biomech</addtitle><description>Application of lipopolysaccharide (LPS) is a widely employed model to mimic acute respiratory distress syndrome (ARDS). Available data regarding LPS-induced biomechanical changes on pulmonary epithelial cells are limited only to P. aeruginosa LPS. Considering that LPS from different bacteria could promote a specific mechanical response in epithelial cells, we aim to assess the effect of E. coli LPS, widely employed as a model of ARDS, in the biomechanics of alveolar epithelial cells.
Young’s modulus (E) of alveolar epithelial cells (A549) was measured by atomic force microscopy every 5 min throughout 60 min of experiment after treatment with LPS from E. coli (100 μg/mL). The percentage of cells presenting actin stress fibers (F-actin staining) was also evaluated. Control cells were treated with culture medium and the values obtained were compared with LPS-treated cells for each time-point.
Application of LPS induced significant increase in E after 20 min (77%) till 60 min (104%) in comparison to controls. Increase in lung epithelial cell stiffness induced by LPS was associated with a higher number of cells presenting cytoskeletal remodeling.
The observed effects of E. coli LPS on alveolar epithelial cells suggest that this widely-used LPS is able to promote a quick formation of actin stress fibers and stiffening cells, thereby facilitating the disruption of the pulmonary epithelial barrier.</description><subject>Actin</subject><subject>Acute respiratory distress syndrome model</subject><subject>Alveolar epithelium</subject><subject>Alveoli</subject><subject>Atomic force microscopy</subject><subject>Biomechanics</subject><subject>Cell culture</subject><subject>Cytoskeleton</subject><subject>Disruption</subject><subject>E coli</subject><subject>Epithelial cells</subject><subject>Escherichia coli</subject><subject>Inflammation</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides</subject><subject>Lungs</subject><subject>Mechanical analysis</subject><subject>Mechanical properties</subject><subject>Modulus of elasticity</subject><subject>Pseudomonas aeruginosa</subject><subject>Respiratory distress syndrome</subject><subject>Stiffening</subject><subject>Stiffness</subject><subject>Variance analysis</subject><issn>0021-9290</issn><issn>1873-2380</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkE1v1TAQRS0Eoq-Fv1BFYsMmYcbOh7MDVYUiVeoG1pYzmRBHfnGwk0r99-TptSzYsJrNuXeujhDXCAUC1p-mYupcODKNhQTUBWIBqnwlDqgblUul4bU4AEjMW9nChbhMaQKApmzat-JCQSUbpduDeLhNNHJ0NDqbUfAu824JS_BPyRKNNrqeMzf3G3HKrH_k4G3MeHHryN5ZnxF7n6XVDQPPbv71TrwZrE_8_vleiZ9fb3_c3OX3D9--33y5z6lUas1lZXU3tAqkbbQkqBRSpwcJfakrS1wDD1KWmlFaW3NHTSUHxn11Az0OpK7Ex3PvEsPvjdNqji6dttiZw5aMxKrCqlbQ7uiHf9ApbHHe1-1UXaLUdXmi6jNFMaQUeTBLdEcbnwyCOSk3k3lRbk7KDaLZle_B6-f6rTty_zf24ngHPp8B3n08Oo4mkeOZuHeRaTV9cP_78Qe0MpXd</recordid><startdate>20190123</startdate><enddate>20190123</enddate><creator>Oliveira, Vinícius Rosa</creator><creator>Uriarte, Juan José</creator><creator>Falcones, Bryan</creator><creator>Zin, Walter Araujo</creator><creator>Navajas, Daniel</creator><creator>Farré, Ramon</creator><creator>Almendros, Isaac</creator><general>Elsevier Ltd</general><general>Elsevier Limited</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7TB</scope><scope>7TS</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>M7P</scope><scope>MBDVC</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20190123</creationdate><title>Escherichia coli lipopolysaccharide induces alveolar epithelial cell stiffening</title><author>Oliveira, Vinícius Rosa ; Uriarte, Juan José ; Falcones, Bryan ; Zin, Walter Araujo ; Navajas, Daniel ; Farré, Ramon ; Almendros, Isaac</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-25a8bf9302a782c0531cb8f20d485ace60ef2248e12aa6ebc752fe152770d1fc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Actin</topic><topic>Acute respiratory distress syndrome model</topic><topic>Alveolar epithelium</topic><topic>Alveoli</topic><topic>Atomic force microscopy</topic><topic>Biomechanics</topic><topic>Cell culture</topic><topic>Cytoskeleton</topic><topic>Disruption</topic><topic>E coli</topic><topic>Epithelial cells</topic><topic>Escherichia coli</topic><topic>Inflammation</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides</topic><topic>Lungs</topic><topic>Mechanical analysis</topic><topic>Mechanical properties</topic><topic>Modulus of elasticity</topic><topic>Pseudomonas aeruginosa</topic><topic>Respiratory distress syndrome</topic><topic>Stiffening</topic><topic>Stiffness</topic><topic>Variance analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oliveira, Vinícius Rosa</creatorcontrib><creatorcontrib>Uriarte, Juan José</creatorcontrib><creatorcontrib>Falcones, Bryan</creatorcontrib><creatorcontrib>Zin, Walter Araujo</creatorcontrib><creatorcontrib>Navajas, Daniel</creatorcontrib><creatorcontrib>Farré, Ramon</creatorcontrib><creatorcontrib>Almendros, Isaac</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Physical Education Index</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Research Library</collection><collection>Biological Science Database</collection><collection>Research Library (Corporate)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomechanics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oliveira, Vinícius Rosa</au><au>Uriarte, Juan José</au><au>Falcones, Bryan</au><au>Zin, Walter Araujo</au><au>Navajas, Daniel</au><au>Farré, Ramon</au><au>Almendros, Isaac</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Escherichia coli lipopolysaccharide induces alveolar epithelial cell stiffening</atitle><jtitle>Journal of biomechanics</jtitle><addtitle>J Biomech</addtitle><date>2019-01-23</date><risdate>2019</risdate><volume>83</volume><spage>315</spage><epage>318</epage><pages>315-318</pages><issn>0021-9290</issn><eissn>1873-2380</eissn><abstract>Application of lipopolysaccharide (LPS) is a widely employed model to mimic acute respiratory distress syndrome (ARDS). Available data regarding LPS-induced biomechanical changes on pulmonary epithelial cells are limited only to P. aeruginosa LPS. Considering that LPS from different bacteria could promote a specific mechanical response in epithelial cells, we aim to assess the effect of E. coli LPS, widely employed as a model of ARDS, in the biomechanics of alveolar epithelial cells.
Young’s modulus (E) of alveolar epithelial cells (A549) was measured by atomic force microscopy every 5 min throughout 60 min of experiment after treatment with LPS from E. coli (100 μg/mL). The percentage of cells presenting actin stress fibers (F-actin staining) was also evaluated. Control cells were treated with culture medium and the values obtained were compared with LPS-treated cells for each time-point.
Application of LPS induced significant increase in E after 20 min (77%) till 60 min (104%) in comparison to controls. Increase in lung epithelial cell stiffness induced by LPS was associated with a higher number of cells presenting cytoskeletal remodeling.
The observed effects of E. coli LPS on alveolar epithelial cells suggest that this widely-used LPS is able to promote a quick formation of actin stress fibers and stiffening cells, thereby facilitating the disruption of the pulmonary epithelial barrier.</abstract><cop>United States</cop><pub>Elsevier Ltd</pub><pmid>30527389</pmid><doi>10.1016/j.jbiomech.2018.11.034</doi><tpages>4</tpages></addata></record> |
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| subjects | Actin Acute respiratory distress syndrome model Alveolar epithelium Alveoli Atomic force microscopy Biomechanics Cell culture Cytoskeleton Disruption E coli Epithelial cells Escherichia coli Inflammation Lipopolysaccharide Lipopolysaccharides Lungs Mechanical analysis Mechanical properties Modulus of elasticity Pseudomonas aeruginosa Respiratory distress syndrome Stiffening Stiffness Variance analysis |
| title | Escherichia coli lipopolysaccharide induces alveolar epithelial cell stiffening |
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