Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum
In this study therapeutic potential of fucoidan fraction-2 (Fu-F2) isolated from Sargassum polycystum was evaluated for the development of antibacterial agent against the human and animal pathogenic bacteria by in vitro and in vivo analysis. The Fu-F2 contained 51.12 ± 0.86% of total sugar and 20.41...
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| Veröffentlicht in: | International journal of biological macromolecules 2019-03, Vol.125, p.485-495 |
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| creator | Palanisamy, Subramanian Vinosha, Manoharan Rajasekar, Periyannan Anjali, Ravichandran Sathiyaraj, Ganesan Marudhupandi, Thangapandi Selvam, Samayanan Prabhu, Narayanasamy Marimuthu You, SangGuan |
| description | In this study therapeutic potential of fucoidan fraction-2 (Fu-F2) isolated from Sargassum polycystum was evaluated for the development of antibacterial agent against the human and animal pathogenic bacteria by in vitro and in vivo analysis. The Fu-F2 contained 51.12 ± 0.86% of total sugar and 20.41 ± 0.91% of sulfate. The structural characterization of Fu-F2 was performed by HPLC, FTIR and NMR analysis and reported in our earlier study. The in vitro antibacterial assays such as MIC, MBC, killing kinetics, disk diffusion, protein leakage, ROS and confocal laser scanning microscopy demonstrate that Fu-F2 possesses the highest antibacterial activity against the tested pathogens. Among the tested pathogens, the highest antibacterial activity (21 ± 1.0 mm) was recorded at the concentration of 50 μg/ml against Pseudomonas aeruginosa and the lowest activity (16 ± 0.53 mm) was registered against Staphylococcus aureus. In the in vivo analysis, the pre-treatment group with Fu-F2 at the concentration of 15 mg/0.1 kg through feed exhibited the highest survival (83.4%) and antioxidant activities (p |
| doi_str_mv | 10.1016/j.ijbiomac.2018.12.070 |
| format | Article |
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•The fucoidan fraction-2 (Fu-F2) was successfully isolated from Sargassum polycystum.•The antibacterial activity of Fu-F2 was tested by various in vitro and in vivo assays.•The Fu-F2 shows higher antibacterial activity against P. aeruginosa than the other bacteria.•The in vivo studies confirmed that Fu-F2 treated zebrafish shows excellent antioxidant properties and improved the histological architecture.</description><identifier>ISSN: 0141-8130</identifier><identifier>EISSN: 1879-0003</identifier><identifier>DOI: 10.1016/j.ijbiomac.2018.12.070</identifier><identifier>PMID: 30529554</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - chemistry ; Anti-Bacterial Agents - pharmacology ; Antibacterial activity ; Antioxidants - chemistry ; Antioxidants - pharmacology ; Bacteria - drug effects ; Biological Products - chemistry ; Biological Products - pharmacology ; Fu-F2 ; Humans ; Microbial Sensitivity Tests - methods ; Polysaccharides - chemistry ; Polysaccharides - pharmacology ; Sargassum - chemistry ; Sargassum polycystum ; Sulfates - chemistry</subject><ispartof>International journal of biological macromolecules, 2019-03, Vol.125, p.485-495</ispartof><rights>2018 Elsevier B.V.</rights><rights>Copyright © 2018 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c368t-8464c8d2d90b692770d167f052c2255f5a0bd29a08c936b53ac8ad328f1bbada3</citedby><cites>FETCH-LOGICAL-c368t-8464c8d2d90b692770d167f052c2255f5a0bd29a08c936b53ac8ad328f1bbada3</cites><orcidid>0000-0003-3227-1158</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S014181301836094X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30529554$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Palanisamy, Subramanian</creatorcontrib><creatorcontrib>Vinosha, Manoharan</creatorcontrib><creatorcontrib>Rajasekar, Periyannan</creatorcontrib><creatorcontrib>Anjali, Ravichandran</creatorcontrib><creatorcontrib>Sathiyaraj, Ganesan</creatorcontrib><creatorcontrib>Marudhupandi, Thangapandi</creatorcontrib><creatorcontrib>Selvam, Samayanan</creatorcontrib><creatorcontrib>Prabhu, Narayanasamy Marimuthu</creatorcontrib><creatorcontrib>You, SangGuan</creatorcontrib><title>Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum</title><title>International journal of biological macromolecules</title><addtitle>Int J Biol Macromol</addtitle><description>In this study therapeutic potential of fucoidan fraction-2 (Fu-F2) isolated from Sargassum polycystum was evaluated for the development of antibacterial agent against the human and animal pathogenic bacteria by in vitro and in vivo analysis. The Fu-F2 contained 51.12 ± 0.86% of total sugar and 20.41 ± 0.91% of sulfate. The structural characterization of Fu-F2 was performed by HPLC, FTIR and NMR analysis and reported in our earlier study. The in vitro antibacterial assays such as MIC, MBC, killing kinetics, disk diffusion, protein leakage, ROS and confocal laser scanning microscopy demonstrate that Fu-F2 possesses the highest antibacterial activity against the tested pathogens. Among the tested pathogens, the highest antibacterial activity (21 ± 1.0 mm) was recorded at the concentration of 50 μg/ml against Pseudomonas aeruginosa and the lowest activity (16 ± 0.53 mm) was registered against Staphylococcus aureus. In the in vivo analysis, the pre-treatment group with Fu-F2 at the concentration of 15 mg/0.1 kg through feed exhibited the highest survival (83.4%) and antioxidant activities (p < 0.05) than the fish infected with pathogen. Thus, the present findings suggest that the Fu-F2 of S. polycystum encompasses significant antibacterial properties and that can be used as a therapeutic agent for controlling the bacterial disease.
•The fucoidan fraction-2 (Fu-F2) was successfully isolated from Sargassum polycystum.•The antibacterial activity of Fu-F2 was tested by various in vitro and in vivo assays.•The Fu-F2 shows higher antibacterial activity against P. aeruginosa than the other bacteria.•The in vivo studies confirmed that Fu-F2 treated zebrafish shows excellent antioxidant properties and improved the histological architecture.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - chemistry</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Antibacterial activity</subject><subject>Antioxidants - chemistry</subject><subject>Antioxidants - pharmacology</subject><subject>Bacteria - drug effects</subject><subject>Biological Products - chemistry</subject><subject>Biological Products - pharmacology</subject><subject>Fu-F2</subject><subject>Humans</subject><subject>Microbial Sensitivity Tests - methods</subject><subject>Polysaccharides - chemistry</subject><subject>Polysaccharides - pharmacology</subject><subject>Sargassum - chemistry</subject><subject>Sargassum polycystum</subject><subject>Sulfates - chemistry</subject><issn>0141-8130</issn><issn>1879-0003</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkEtv1DAQgC0EokvhL1Q-lkPCjLNOnBtVxbZIlThADz1ZEz-QV0m82Ali_z1ebcuV00gz37w-xq4QagRsP-3rsB9CnMjUAlDVKGro4BXboOr6CgCa12wDuMVKYQMX7F3O-5JtJaq37KIBKXoptxv2dDMvYSCzuBRo5M77YMgcefScuF9NDJZm7lMhQpz59W6tduIjd3-WU8rZUooT_07pJ-W8TvwQx6M55mWd3rM3nsbsPjzHS_a4-_Lj9r56-Hb39fbmoTJNq5ZKbdutUVbYHoa2F10HFtvOlwONEFJ6STBY0RMo0zftIBsyimwjlMdhIEvNJbs-zz2k-Gt1edFTyMaNI80urlkLlBKlQFAFbc-oSTHn5Lw-pDBROmoEfdKq9_pFqz5p1Sh00Voar553rMPk7L-2F48F-HwGXPn0d3BJZxPcbJwNyZlF2xj-t-MvcO-MLg</recordid><startdate>20190315</startdate><enddate>20190315</enddate><creator>Palanisamy, Subramanian</creator><creator>Vinosha, Manoharan</creator><creator>Rajasekar, Periyannan</creator><creator>Anjali, Ravichandran</creator><creator>Sathiyaraj, Ganesan</creator><creator>Marudhupandi, Thangapandi</creator><creator>Selvam, Samayanan</creator><creator>Prabhu, Narayanasamy Marimuthu</creator><creator>You, SangGuan</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3227-1158</orcidid></search><sort><creationdate>20190315</creationdate><title>Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum</title><author>Palanisamy, Subramanian ; Vinosha, Manoharan ; Rajasekar, Periyannan ; Anjali, Ravichandran ; Sathiyaraj, Ganesan ; Marudhupandi, Thangapandi ; Selvam, Samayanan ; Prabhu, Narayanasamy Marimuthu ; You, SangGuan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c368t-8464c8d2d90b692770d167f052c2255f5a0bd29a08c936b53ac8ad328f1bbada3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - chemistry</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Antibacterial activity</topic><topic>Antioxidants - chemistry</topic><topic>Antioxidants - pharmacology</topic><topic>Bacteria - drug effects</topic><topic>Biological Products - chemistry</topic><topic>Biological Products - pharmacology</topic><topic>Fu-F2</topic><topic>Humans</topic><topic>Microbial Sensitivity Tests - methods</topic><topic>Polysaccharides - chemistry</topic><topic>Polysaccharides - pharmacology</topic><topic>Sargassum - chemistry</topic><topic>Sargassum polycystum</topic><topic>Sulfates - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Palanisamy, Subramanian</creatorcontrib><creatorcontrib>Vinosha, Manoharan</creatorcontrib><creatorcontrib>Rajasekar, Periyannan</creatorcontrib><creatorcontrib>Anjali, Ravichandran</creatorcontrib><creatorcontrib>Sathiyaraj, Ganesan</creatorcontrib><creatorcontrib>Marudhupandi, Thangapandi</creatorcontrib><creatorcontrib>Selvam, Samayanan</creatorcontrib><creatorcontrib>Prabhu, Narayanasamy Marimuthu</creatorcontrib><creatorcontrib>You, SangGuan</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of biological macromolecules</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Palanisamy, Subramanian</au><au>Vinosha, Manoharan</au><au>Rajasekar, Periyannan</au><au>Anjali, Ravichandran</au><au>Sathiyaraj, Ganesan</au><au>Marudhupandi, Thangapandi</au><au>Selvam, Samayanan</au><au>Prabhu, Narayanasamy Marimuthu</au><au>You, SangGuan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum</atitle><jtitle>International journal of biological macromolecules</jtitle><addtitle>Int J Biol Macromol</addtitle><date>2019-03-15</date><risdate>2019</risdate><volume>125</volume><spage>485</spage><epage>495</epage><pages>485-495</pages><issn>0141-8130</issn><eissn>1879-0003</eissn><abstract>In this study therapeutic potential of fucoidan fraction-2 (Fu-F2) isolated from Sargassum polycystum was evaluated for the development of antibacterial agent against the human and animal pathogenic bacteria by in vitro and in vivo analysis. The Fu-F2 contained 51.12 ± 0.86% of total sugar and 20.41 ± 0.91% of sulfate. The structural characterization of Fu-F2 was performed by HPLC, FTIR and NMR analysis and reported in our earlier study. The in vitro antibacterial assays such as MIC, MBC, killing kinetics, disk diffusion, protein leakage, ROS and confocal laser scanning microscopy demonstrate that Fu-F2 possesses the highest antibacterial activity against the tested pathogens. Among the tested pathogens, the highest antibacterial activity (21 ± 1.0 mm) was recorded at the concentration of 50 μg/ml against Pseudomonas aeruginosa and the lowest activity (16 ± 0.53 mm) was registered against Staphylococcus aureus. In the in vivo analysis, the pre-treatment group with Fu-F2 at the concentration of 15 mg/0.1 kg through feed exhibited the highest survival (83.4%) and antioxidant activities (p < 0.05) than the fish infected with pathogen. Thus, the present findings suggest that the Fu-F2 of S. polycystum encompasses significant antibacterial properties and that can be used as a therapeutic agent for controlling the bacterial disease.
•The fucoidan fraction-2 (Fu-F2) was successfully isolated from Sargassum polycystum.•The antibacterial activity of Fu-F2 was tested by various in vitro and in vivo assays.•The Fu-F2 shows higher antibacterial activity against P. aeruginosa than the other bacteria.•The in vivo studies confirmed that Fu-F2 treated zebrafish shows excellent antioxidant properties and improved the histological architecture.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30529554</pmid><doi>10.1016/j.ijbiomac.2018.12.070</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0003-3227-1158</orcidid></addata></record> |
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| subjects | Animals Anti-Bacterial Agents - chemistry Anti-Bacterial Agents - pharmacology Antibacterial activity Antioxidants - chemistry Antioxidants - pharmacology Bacteria - drug effects Biological Products - chemistry Biological Products - pharmacology Fu-F2 Humans Microbial Sensitivity Tests - methods Polysaccharides - chemistry Polysaccharides - pharmacology Sargassum - chemistry Sargassum polycystum Sulfates - chemistry |
| title | Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum |
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