Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine
The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding forma...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2008-08, Vol.74 (15), p.4847-4852 |
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| creator | Matthies, Anastasia Clavel, Thomas Gütschow, Michael Engst, Wolfram Haller, Dirk Blaut, Michael Braune, Annett |
| description | The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the CORIOBACTERIACEAE: Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones. |
| doi_str_mv | 10.1128/aem.00555-08 |
| format | Article |
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Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the CORIOBACTERIACEAE: Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/aem.00555-08</identifier><identifier>PMID: 18539813</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacteria ; Bacteria, Anaerobic - isolation & purification ; Bacteria, Anaerobic - metabolism ; Biodegradation ; Biological and medical sciences ; Biotransformation ; Chromatography, High Pressure Liquid ; Dietary supplements ; Digestive system ; Digestive tract ; Enzymes ; Female ; Fundamental and applied biological sciences. Psychology ; Genistein - metabolism ; Gram-positive bacteria ; Gram-Positive Bacteria - isolation & purification ; Gram-Positive Bacteria - metabolism ; Ileum - microbiology ; Isoflavones - metabolism ; Metabolism ; Mice ; Mice, Inbred C57BL ; Microbiology ; Molecular Sequence Data ; Rodents ; Spectrometry, Mass, Electrospray Ionization</subject><ispartof>Applied and Environmental Microbiology, 2008-08, Vol.74 (15), p.4847-4852</ispartof><rights>2008 INIST-CNRS</rights><rights>Copyright American Society for Microbiology Aug 2008</rights><rights>Copyright © 2008, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c587t-ba1c631edf37041d134473cf70b53df067464a82a91c47fcd2aa2ae44351e123</citedby><cites>FETCH-LOGICAL-c587t-ba1c631edf37041d134473cf70b53df067464a82a91c47fcd2aa2ae44351e123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519357/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2519357/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,3188,3189,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20558779$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18539813$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matthies, Anastasia</creatorcontrib><creatorcontrib>Clavel, Thomas</creatorcontrib><creatorcontrib>Gütschow, Michael</creatorcontrib><creatorcontrib>Engst, Wolfram</creatorcontrib><creatorcontrib>Haller, Dirk</creatorcontrib><creatorcontrib>Blaut, Michael</creatorcontrib><creatorcontrib>Braune, Annett</creatorcontrib><title>Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the CORIOBACTERIACEAE: Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.</description><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteria, Anaerobic - isolation & purification</subject><subject>Bacteria, Anaerobic - metabolism</subject><subject>Biodegradation</subject><subject>Biological and medical sciences</subject><subject>Biotransformation</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Dietary supplements</subject><subject>Digestive system</subject><subject>Digestive tract</subject><subject>Enzymes</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genistein - metabolism</subject><subject>Gram-positive bacteria</subject><subject>Gram-Positive Bacteria - isolation & purification</subject><subject>Gram-Positive Bacteria - metabolism</subject><subject>Ileum - microbiology</subject><subject>Isoflavones - metabolism</subject><subject>Metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Rodents</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdks1vEzEUxFcIREPhxhksJDixxZ9Z7wUphFIitXCgnK0X73Piatdu7U2r8NfjkKgFTpbtn8YznldVLxk9YYzrD4DDCaVKqZrqR9WE0VbXSojp42pCadvWnEt6VD3L-YpSKulUP62OmFai1UxMKjeP4RZT9jGQ6Mhn8N0v9IFA6MgZBp_H3W65LQdkFgBTXHpLPoEdMfnNQL7hXb8lixx7GLEjLsWBjGskF3GTkSzCiHn0AZ9XTxz0GV8c1uPq8svp5fxrff79bDGfnddW6Wasl8DsVDDsnGioZB0TUjbCuoYulegcnTZyKkFzaJmVjbMdB-CAUgrFkHFxXH3cy15vlgN2FsOYoDfXyQ-QtiaCN__eBL82q3hruGKtUE0ReHcQSPFmU7ybwWeLfQ8BSyLDmaJcU1nAN_-BV3GTQslmOFVtQ4tcgd7vIZtizgndvRNGza48Mzu9MH_KM1QX_NXf7h_gQ1sFeHsAIFvoXYJgfb7nysPlF5v2wdzar9Z3PqGBPJgyKKaRhikjtdxFfb2HHEQDq1SEfv7glIkyNbyMChO_AZnsts4</recordid><startdate>20080801</startdate><enddate>20080801</enddate><creator>Matthies, Anastasia</creator><creator>Clavel, Thomas</creator><creator>Gütschow, Michael</creator><creator>Engst, Wolfram</creator><creator>Haller, Dirk</creator><creator>Blaut, Michael</creator><creator>Braune, Annett</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>5PM</scope></search><sort><creationdate>20080801</creationdate><title>Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine</title><author>Matthies, Anastasia ; Clavel, Thomas ; Gütschow, Michael ; Engst, Wolfram ; Haller, Dirk ; Blaut, Michael ; Braune, Annett</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c587t-ba1c631edf37041d134473cf70b53df067464a82a91c47fcd2aa2ae44351e123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteria, Anaerobic - isolation & purification</topic><topic>Bacteria, Anaerobic - metabolism</topic><topic>Biodegradation</topic><topic>Biological and medical sciences</topic><topic>Biotransformation</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Dietary supplements</topic><topic>Digestive system</topic><topic>Digestive tract</topic><topic>Enzymes</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genistein - metabolism</topic><topic>Gram-positive bacteria</topic><topic>Gram-Positive Bacteria - isolation & purification</topic><topic>Gram-Positive Bacteria - metabolism</topic><topic>Ileum - microbiology</topic><topic>Isoflavones - metabolism</topic><topic>Metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Rodents</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matthies, Anastasia</creatorcontrib><creatorcontrib>Clavel, Thomas</creatorcontrib><creatorcontrib>Gütschow, Michael</creatorcontrib><creatorcontrib>Engst, Wolfram</creatorcontrib><creatorcontrib>Haller, Dirk</creatorcontrib><creatorcontrib>Blaut, Michael</creatorcontrib><creatorcontrib>Braune, Annett</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matthies, Anastasia</au><au>Clavel, Thomas</au><au>Gütschow, Michael</au><au>Engst, Wolfram</au><au>Haller, Dirk</au><au>Blaut, Michael</au><au>Braune, Annett</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2008-08-01</date><risdate>2008</risdate><volume>74</volume><issue>15</issue><spage>4847</spage><epage>4852</epage><pages>4847-4852</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>The metabolism of isoflavones by gut bacteria plays a key role in the availability and bioactivation of these compounds in the intestine. Daidzein and genistein are the most common dietary soy isoflavones. While daidzein conversion yielding equol has been known for some time, the corresponding formation of 5-hydroxy-equol from genistein has not been reported previously. We isolated a strictly anaerobic bacterium (Mt1B8) from the mouse intestine which converted daidzein via dihydrodaidzein to equol as well as genistein via dihydrogenistein to 5-hydroxy-equol. Strain Mt1B8 was a gram-positive, rod-shaped bacterium identified as a member of the CORIOBACTERIACEAE: Strain Mt1B8 also transformed dihydrodaidzein and dihydrogenistein to equol and 5-hydroxy-equol, respectively. The conversion of daidzein, genistein, dihydrodaidzein, and dihydrogenistein in the stationary growth phase depended on preincubation with the corresponding isoflavonoid, indicating enzyme induction. Moreover, dihydrogenistein was transformed even more rapidly in the stationary phase when strain Mt1B8 was grown on either genistein or daidzein. Growing the cells on daidzein also enabled conversion of genistein. This suggests that the same enzymes are involved in the conversion of the two isoflavones.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>18539813</pmid><doi>10.1128/aem.00555-08</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bacteria Bacteria, Anaerobic - isolation & purification Bacteria, Anaerobic - metabolism Biodegradation Biological and medical sciences Biotransformation Chromatography, High Pressure Liquid Dietary supplements Digestive system Digestive tract Enzymes Female Fundamental and applied biological sciences. Psychology Genistein - metabolism Gram-positive bacteria Gram-Positive Bacteria - isolation & purification Gram-Positive Bacteria - metabolism Ileum - microbiology Isoflavones - metabolism Metabolism Mice Mice, Inbred C57BL Microbiology Molecular Sequence Data Rodents Spectrometry, Mass, Electrospray Ionization |
| title | Conversion of Daidzein and Genistein by an Anaerobic Bacterium Newly Isolated from the Mouse Intestine |
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