Continuous monitoring of the activation and activity of [NiFe]-hydrogenases by membrane-inlet mass spectrometry

The hydrogen–deuterium (H +/D 2) exchange reaction catalysed by [NiFe]-hydrogenases in the D 2/H 2O system has been used to study enzyme activation and activity by membrane-inlet mass spectrometry. The activation of the [NiFe]-hydrogenases from Thiocapsa roseopersicina (HynSL), Desulfovibrio fructos...

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Veröffentlicht in:	International journal of hydrogen energy 2002-01, Vol.27 (11), p.1441-1448
Hauptverfasser:	Vignais, Paulette M., Cournac, Laurent, Hatchikian, E.Claude, Elsen, Sylvie, Serebryakova, Larissa, Zorin, Nikolay, Dimon, Bernard
Format:	Artikel
Sprache:	eng
Schlagworte:	[NiFe] hydrogenases Catalyst activity Deuterium Enzymes H +/D 2 exchange reaction Hydrogenase activation Hydrogenase catalytic mechanism Mass spectrometry
Online-Zugang:	Volltext
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