Drosophila melanogaster Mounts a Unique Immune Response to the Rhabdovirus Sigma virus
Rhabdoviruses are important pathogens of humans, livestock, and plants that are often vectored by insects. Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a na...
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| Veröffentlicht in: | Applied and Environmental Microbiology 2008-05, Vol.74 (10), p.3251-3256 |
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| creator | Tsai, C.W McGraw, E.A Ammar, E.-D Dietzgen, R.G Hogenhout, S.A |
| description | Rhabdoviruses are important pathogens of humans, livestock, and plants that are often vectored by insects. Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a naturally occurring disease agent of Drosophila melanogaster. The infection is maintained in Drosophila populations through vertical transmission via germ cells. We report here the nature of the Drosophila innate immune response to SIGMAV infection as revealed by quantitative reverse transcription-PCR analysis of differentially expressed genes identified by microarray analysis. We have also compared and contrasted the immune response of the host with respect to two nonenveloped viruses, Drosophila C virus (DCV) and Drosophila X virus (DXV). We determined that SIGMAV infection upregulates expression of the peptidoglycan receptor protein genes PGRP-SB1 and PGRP-SD and the antimicrobial peptide (AMP) genes Diptericin-A, Attacin-A, Attacin-B, Cecropin-A1, and DROSOCIN: SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections. Expression levels of the Toll and Imd signaling cascade genes are not significantly altered by SIGMAV infection. These results highlight shared and unique aspects of the Drosophila immune response to the three viruses and may shed light on the nature of the interaction with the host and the evolution of these associations. |
| doi_str_mv | 10.1128/AEM.02248-07 |
| format | Article |
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Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a naturally occurring disease agent of Drosophila melanogaster. The infection is maintained in Drosophila populations through vertical transmission via germ cells. We report here the nature of the Drosophila innate immune response to SIGMAV infection as revealed by quantitative reverse transcription-PCR analysis of differentially expressed genes identified by microarray analysis. We have also compared and contrasted the immune response of the host with respect to two nonenveloped viruses, Drosophila C virus (DCV) and Drosophila X virus (DXV). We determined that SIGMAV infection upregulates expression of the peptidoglycan receptor protein genes PGRP-SB1 and PGRP-SD and the antimicrobial peptide (AMP) genes Diptericin-A, Attacin-A, Attacin-B, Cecropin-A1, and DROSOCIN: SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections. Expression levels of the Toll and Imd signaling cascade genes are not significantly altered by SIGMAV infection. These results highlight shared and unique aspects of the Drosophila immune response to the three viruses and may shed light on the nature of the interaction with the host and the evolution of these associations.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.02248-07</identifier><identifier>PMID: 18378641</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Antimicrobial Cationic Peptides - biosynthesis ; Antimicrobial Cationic Peptides - genetics ; Antimicrobial peptides ; Biological and medical sciences ; Carrier Proteins - biosynthesis ; Carrier Proteins - genetics ; Drosophila C virus ; Drosophila melanogaster - immunology ; Female ; Fundamental and applied biological sciences. 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Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a naturally occurring disease agent of Drosophila melanogaster. The infection is maintained in Drosophila populations through vertical transmission via germ cells. We report here the nature of the Drosophila innate immune response to SIGMAV infection as revealed by quantitative reverse transcription-PCR analysis of differentially expressed genes identified by microarray analysis. We have also compared and contrasted the immune response of the host with respect to two nonenveloped viruses, Drosophila C virus (DCV) and Drosophila X virus (DXV). We determined that SIGMAV infection upregulates expression of the peptidoglycan receptor protein genes PGRP-SB1 and PGRP-SD and the antimicrobial peptide (AMP) genes Diptericin-A, Attacin-A, Attacin-B, Cecropin-A1, and DROSOCIN: SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections. Expression levels of the Toll and Imd signaling cascade genes are not significantly altered by SIGMAV infection. 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Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Glycoproteins</subject><subject>Infections</subject><subject>Insects</subject><subject>Invertebrate Microbiology</subject><subject>Male</subject><subject>Microbiology</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Peptides</subject><subject>Rhabdoviridae - immunology</subject><subject>Signal Transduction - genetics</subject><subject>Viruses</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EokvhxhksJDiRMv5K7AtSVQpUaoVEWa6Wk04SV0m82EkR_z3e7qoFLpxsa356nvceIc8ZHDHG9bvj04sj4FzqAqoHZMXA6EIJUT4kKwBjijyCA_IkpWsAkFDqx-SAaVHpUrIV-f4hhhQ2vR8cHXFwU-hcmjHSi7BMc6KOrif_Y0F6No7LhPQrpk2YEtI50LnP797VV-HGxyXRS9-Njt7en5JHrRsSPtufh2T98fTbyefi_Muns5Pj86KRWswFK5VUDlWtZC25QKZLAxVXtcRayMZUrC3L1rRO1aY1RiBWpWsFr0BwA4jikLzf6W6WesSrBqc5usFuoh9d_GWD8_bvyeR724Uby4WRRqks8GYvEEO2mWY7-tTgkJPAsCRbGmYU1_BfkDNpDAOZwVf_gNdhiVNOwXJQ2ZKQJkNvd1CT408R27uVGdhtrTbXam9rtVBl_MWfNu_hfY8ZeL0HXGrc0EY3NT7dcRyEzFr8frned_1PH9G6NFqHo63k9mvB1Vbs5Q5qXbCui1lofcmBCQBthDZS_Abaxb6u</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Tsai, C.W</creator><creator>McGraw, E.A</creator><creator>Ammar, E.-D</creator><creator>Dietzgen, R.G</creator><creator>Hogenhout, S.A</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7SN</scope><scope>7SS</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>SOI</scope><scope>7T5</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20080501</creationdate><title>Drosophila melanogaster Mounts a Unique Immune Response to the Rhabdovirus Sigma virus</title><author>Tsai, C.W ; McGraw, E.A ; Ammar, E.-D ; Dietzgen, R.G ; Hogenhout, S.A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c483t-16545ae5b54b423e18690725b4eb34c971f66f9fa5b9f993ee76af32703290ee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antimicrobial Cationic Peptides - biosynthesis</topic><topic>Antimicrobial Cationic Peptides - genetics</topic><topic>Antimicrobial peptides</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - biosynthesis</topic><topic>Carrier Proteins - genetics</topic><topic>Drosophila C virus</topic><topic>Drosophila melanogaster - immunology</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Glycoproteins</topic><topic>Infections</topic><topic>Insects</topic><topic>Invertebrate Microbiology</topic><topic>Male</topic><topic>Microbiology</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Peptides</topic><topic>Rhabdoviridae - immunology</topic><topic>Signal Transduction - genetics</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsai, C.W</creatorcontrib><creatorcontrib>McGraw, E.A</creatorcontrib><creatorcontrib>Ammar, E.-D</creatorcontrib><creatorcontrib>Dietzgen, R.G</creatorcontrib><creatorcontrib>Hogenhout, S.A</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>Immunology Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsai, C.W</au><au>McGraw, E.A</au><au>Ammar, E.-D</au><au>Dietzgen, R.G</au><au>Hogenhout, S.A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Drosophila melanogaster Mounts a Unique Immune Response to the Rhabdovirus Sigma virus</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>74</volume><issue>10</issue><spage>3251</spage><epage>3256</epage><pages>3251-3256</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>Rhabdoviruses are important pathogens of humans, livestock, and plants that are often vectored by insects. Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a naturally occurring disease agent of Drosophila melanogaster. The infection is maintained in Drosophila populations through vertical transmission via germ cells. We report here the nature of the Drosophila innate immune response to SIGMAV infection as revealed by quantitative reverse transcription-PCR analysis of differentially expressed genes identified by microarray analysis. We have also compared and contrasted the immune response of the host with respect to two nonenveloped viruses, Drosophila C virus (DCV) and Drosophila X virus (DXV). We determined that SIGMAV infection upregulates expression of the peptidoglycan receptor protein genes PGRP-SB1 and PGRP-SD and the antimicrobial peptide (AMP) genes Diptericin-A, Attacin-A, Attacin-B, Cecropin-A1, and DROSOCIN: SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections. Expression levels of the Toll and Imd signaling cascade genes are not significantly altered by SIGMAV infection. These results highlight shared and unique aspects of the Drosophila immune response to the three viruses and may shed light on the nature of the interaction with the host and the evolution of these associations.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>18378641</pmid><doi>10.1128/AEM.02248-07</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | American Society for Microbiology; MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Animals Antimicrobial Cationic Peptides - biosynthesis Antimicrobial Cationic Peptides - genetics Antimicrobial peptides Biological and medical sciences Carrier Proteins - biosynthesis Carrier Proteins - genetics Drosophila C virus Drosophila melanogaster - immunology Female Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Glycoproteins Infections Insects Invertebrate Microbiology Male Microbiology Oligonucleotide Array Sequence Analysis Peptides Rhabdoviridae - immunology Signal Transduction - genetics Viruses |
| title | Drosophila melanogaster Mounts a Unique Immune Response to the Rhabdovirus Sigma virus |
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