Papaya extract upregulates the immune and antioxidants‐related genes, and proteins expression in milk somatic cells of Friesian dairy cows
Carica papaya is a perennial plant containing bioactive constituents with free radical‐scavenging and immune‐modulating activities. In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of...
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| Veröffentlicht in: | Journal of animal physiology and animal nutrition 2019-03, Vol.103 (2), p.407-415 |
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| container_title | Journal of animal physiology and animal nutrition |
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| creator | Abouzed, Tarek K. Sadek, Kadry M. Ayoub, Mousa M. Saleh, Ebeed A. Nasr, Sherif M. El‐Sayed, Yasser S. Shoukry, Moustafa |
| description | Carica papaya is a perennial plant containing bioactive constituents with free radical‐scavenging and immune‐modulating activities. In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of the experiment reported here was to determine the potential effect of C. papaya aqueous extract (CPE) on milk production traits, and expression of genes and proteins related to immune and antioxidant status in dairy milk somatic cells (MSCs). Forty Friesian dairy cows were divided equally between a control and CPE‐treated groups (orally drenched 250 µg/kg bwt, once weekly a month before expected parturition and continued until 5 months post‐partum). CPE did not affect milk yield or composition but upregulated the expression of β13‐defensin (DEFB13), cathelicidin 2 (CATHL2), cathelicidin antimicrobial peptide (CATHL3), hepcidin (HAMP), lysozyme (LYZ), catalase (CAT) and glutathione peroxidase (GSH‐Px) in MSCs. The environmental micro‐organisms did not influence the levels of the transcripts. The DEFB13, CATHL2, CATHL3, HAMP and LYZ, but not β1‐defensin (DEFB1) transcripts and proteins were constitutively expressed in MSCs obtained from pathogen‐free udders. It could be concluded that CPE has immunostimulant and antioxidant activities; thereby, it could be utilized to minimize the occurrence of mastitis. |
| doi_str_mv | 10.1111/jpn.13032 |
| format | Article |
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In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of the experiment reported here was to determine the potential effect of C. papaya aqueous extract (CPE) on milk production traits, and expression of genes and proteins related to immune and antioxidant status in dairy milk somatic cells (MSCs). Forty Friesian dairy cows were divided equally between a control and CPE‐treated groups (orally drenched 250 µg/kg bwt, once weekly a month before expected parturition and continued until 5 months post‐partum). CPE did not affect milk yield or composition but upregulated the expression of β13‐defensin (DEFB13), cathelicidin 2 (CATHL2), cathelicidin antimicrobial peptide (CATHL3), hepcidin (HAMP), lysozyme (LYZ), catalase (CAT) and glutathione peroxidase (GSH‐Px) in MSCs. The environmental micro‐organisms did not influence the levels of the transcripts. The DEFB13, CATHL2, CATHL3, HAMP and LYZ, but not β1‐defensin (DEFB1) transcripts and proteins were constitutively expressed in MSCs obtained from pathogen‐free udders. It could be concluded that CPE has immunostimulant and antioxidant activities; thereby, it could be utilized to minimize the occurrence of mastitis.</description><identifier>ISSN: 0931-2439</identifier><identifier>EISSN: 1439-0396</identifier><identifier>DOI: 10.1111/jpn.13032</identifier><identifier>PMID: 30511402</identifier><language>eng</language><publisher>Germany: Wiley Subscription Services, Inc</publisher><subject>Adjuvants, Immunologic ; Animal Feed - analysis ; Animal Nutritional Physiological Phenomena ; Animals ; Antioxidants ; Antioxidants - metabolism ; Carica - chemistry ; Carica papaya ; Catalase ; Cattle ; Cattle - genetics ; Cattle - immunology ; Cattle - metabolism ; Dairy cattle ; dairy cows ; Diet - veterinary ; Dietary Supplements ; Female ; Free radicals ; Gene expression ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - immunology ; Genes ; Glutathione ; Glutathione peroxidase ; Hepcidin ; immunostimulant ; Immunostimulants ; Lysozyme ; Mammary gland ; Mammary glands ; Mastitis ; Milk ; Milk - cytology ; Milk - metabolism ; Milk production ; Oxidative stress ; Parturition ; Peroxidase ; Plant Extracts - chemistry ; Plant Extracts - pharmacology ; Proteins ; Scavenging ; Somatic cells ; Up-Regulation - drug effects</subject><ispartof>Journal of animal physiology and animal nutrition, 2019-03, Vol.103 (2), p.407-415</ispartof><rights>2018 Blackwell Verlag GmbH</rights><rights>2018 Blackwell Verlag GmbH.</rights><rights>2019 Blackwell Verlag GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3532-ec4c257a276835fbb106490439544fe1f080833cfbf8c8a2e264a9325007bedd3</citedby><cites>FETCH-LOGICAL-c3532-ec4c257a276835fbb106490439544fe1f080833cfbf8c8a2e264a9325007bedd3</cites><orcidid>0000-0003-3915-4640</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fjpn.13032$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fjpn.13032$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30511402$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Abouzed, Tarek K.</creatorcontrib><creatorcontrib>Sadek, Kadry M.</creatorcontrib><creatorcontrib>Ayoub, Mousa M.</creatorcontrib><creatorcontrib>Saleh, Ebeed A.</creatorcontrib><creatorcontrib>Nasr, Sherif M.</creatorcontrib><creatorcontrib>El‐Sayed, Yasser S.</creatorcontrib><creatorcontrib>Shoukry, Moustafa</creatorcontrib><title>Papaya extract upregulates the immune and antioxidants‐related genes, and proteins expression in milk somatic cells of Friesian dairy cows</title><title>Journal of animal physiology and animal nutrition</title><addtitle>J Anim Physiol Anim Nutr (Berl)</addtitle><description>Carica papaya is a perennial plant containing bioactive constituents with free radical‐scavenging and immune‐modulating activities. In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of the experiment reported here was to determine the potential effect of C. papaya aqueous extract (CPE) on milk production traits, and expression of genes and proteins related to immune and antioxidant status in dairy milk somatic cells (MSCs). Forty Friesian dairy cows were divided equally between a control and CPE‐treated groups (orally drenched 250 µg/kg bwt, once weekly a month before expected parturition and continued until 5 months post‐partum). CPE did not affect milk yield or composition but upregulated the expression of β13‐defensin (DEFB13), cathelicidin 2 (CATHL2), cathelicidin antimicrobial peptide (CATHL3), hepcidin (HAMP), lysozyme (LYZ), catalase (CAT) and glutathione peroxidase (GSH‐Px) in MSCs. The environmental micro‐organisms did not influence the levels of the transcripts. The DEFB13, CATHL2, CATHL3, HAMP and LYZ, but not β1‐defensin (DEFB1) transcripts and proteins were constitutively expressed in MSCs obtained from pathogen‐free udders. It could be concluded that CPE has immunostimulant and antioxidant activities; thereby, it could be utilized to minimize the occurrence of mastitis.</description><subject>Adjuvants, Immunologic</subject><subject>Animal Feed - analysis</subject><subject>Animal Nutritional Physiological Phenomena</subject><subject>Animals</subject><subject>Antioxidants</subject><subject>Antioxidants - metabolism</subject><subject>Carica - chemistry</subject><subject>Carica papaya</subject><subject>Catalase</subject><subject>Cattle</subject><subject>Cattle - genetics</subject><subject>Cattle - immunology</subject><subject>Cattle - metabolism</subject><subject>Dairy cattle</subject><subject>dairy cows</subject><subject>Diet - veterinary</subject><subject>Dietary Supplements</subject><subject>Female</subject><subject>Free radicals</subject><subject>Gene expression</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - immunology</subject><subject>Genes</subject><subject>Glutathione</subject><subject>Glutathione peroxidase</subject><subject>Hepcidin</subject><subject>immunostimulant</subject><subject>Immunostimulants</subject><subject>Lysozyme</subject><subject>Mammary gland</subject><subject>Mammary glands</subject><subject>Mastitis</subject><subject>Milk</subject><subject>Milk - cytology</subject><subject>Milk - metabolism</subject><subject>Milk production</subject><subject>Oxidative stress</subject><subject>Parturition</subject><subject>Peroxidase</subject><subject>Plant Extracts - chemistry</subject><subject>Plant Extracts - pharmacology</subject><subject>Proteins</subject><subject>Scavenging</subject><subject>Somatic cells</subject><subject>Up-Regulation - drug effects</subject><issn>0931-2439</issn><issn>1439-0396</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kcFu1DAQhi0EosvCgRdAlrhQqWnHdpxNjlVFaVEFPcA5cpxJ8ZLYwZOo3VsfgAPP2Cept9tyQMKSNZL16dM__hl7K-BQpHO0Hv2hUKDkM7YQuaoyUFXxnC2gUiKT6WGPvSJaA4iVhuIl21OghchBLtjvSzOajeF4M0VjJz6PEa_m3kxIfPqB3A3D7JEb36Y7uXDj2jTp7vZPxC3V8iv0SAcPxBjDhM5TsiUNkQueO88H1__kFAYzOcst9j3x0PHT6JCc8bw1Lm64Ddf0mr3oTE_45nEu2ffTj99OzrKLr5_OT44vMqu0khna3Eq9MnJVlEp3TSOgyCtIe-o871B0UEKplO2arrSlkSiL3FRKaoBVg22rluzDzpsC_5qRpnpwtA1mPIaZainyqixkkb50yd7_g67DHH1Kl6iyFFpr0Ina31E2BqKIXT1GN5i4qQXU24rqVFH9UFFi3z0a52bA9i_51EkCjnbAtetx839T_fnyy055D4hKnQo</recordid><startdate>201903</startdate><enddate>201903</enddate><creator>Abouzed, Tarek K.</creator><creator>Sadek, Kadry M.</creator><creator>Ayoub, Mousa M.</creator><creator>Saleh, Ebeed A.</creator><creator>Nasr, Sherif M.</creator><creator>El‐Sayed, Yasser S.</creator><creator>Shoukry, Moustafa</creator><general>Wiley Subscription Services, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-3915-4640</orcidid></search><sort><creationdate>201903</creationdate><title>Papaya extract upregulates the immune and antioxidants‐related genes, and proteins expression in milk somatic cells of Friesian dairy cows</title><author>Abouzed, Tarek K. ; Sadek, Kadry M. ; Ayoub, Mousa M. ; Saleh, Ebeed A. ; Nasr, Sherif M. ; El‐Sayed, Yasser S. ; Shoukry, Moustafa</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3532-ec4c257a276835fbb106490439544fe1f080833cfbf8c8a2e264a9325007bedd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Adjuvants, Immunologic</topic><topic>Animal Feed - analysis</topic><topic>Animal Nutritional Physiological Phenomena</topic><topic>Animals</topic><topic>Antioxidants</topic><topic>Antioxidants - metabolism</topic><topic>Carica - chemistry</topic><topic>Carica papaya</topic><topic>Catalase</topic><topic>Cattle</topic><topic>Cattle - genetics</topic><topic>Cattle - immunology</topic><topic>Cattle - metabolism</topic><topic>Dairy cattle</topic><topic>dairy cows</topic><topic>Diet - veterinary</topic><topic>Dietary Supplements</topic><topic>Female</topic><topic>Free radicals</topic><topic>Gene expression</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - immunology</topic><topic>Genes</topic><topic>Glutathione</topic><topic>Glutathione peroxidase</topic><topic>Hepcidin</topic><topic>immunostimulant</topic><topic>Immunostimulants</topic><topic>Lysozyme</topic><topic>Mammary gland</topic><topic>Mammary glands</topic><topic>Mastitis</topic><topic>Milk</topic><topic>Milk - cytology</topic><topic>Milk - metabolism</topic><topic>Milk production</topic><topic>Oxidative stress</topic><topic>Parturition</topic><topic>Peroxidase</topic><topic>Plant Extracts - chemistry</topic><topic>Plant Extracts - pharmacology</topic><topic>Proteins</topic><topic>Scavenging</topic><topic>Somatic cells</topic><topic>Up-Regulation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Abouzed, Tarek K.</creatorcontrib><creatorcontrib>Sadek, Kadry M.</creatorcontrib><creatorcontrib>Ayoub, Mousa M.</creatorcontrib><creatorcontrib>Saleh, Ebeed A.</creatorcontrib><creatorcontrib>Nasr, Sherif M.</creatorcontrib><creatorcontrib>El‐Sayed, Yasser S.</creatorcontrib><creatorcontrib>Shoukry, Moustafa</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of animal physiology and animal nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Abouzed, Tarek K.</au><au>Sadek, Kadry M.</au><au>Ayoub, Mousa M.</au><au>Saleh, Ebeed A.</au><au>Nasr, Sherif M.</au><au>El‐Sayed, Yasser S.</au><au>Shoukry, Moustafa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Papaya extract upregulates the immune and antioxidants‐related genes, and proteins expression in milk somatic cells of Friesian dairy cows</atitle><jtitle>Journal of animal physiology and animal nutrition</jtitle><addtitle>J Anim Physiol Anim Nutr (Berl)</addtitle><date>2019-03</date><risdate>2019</risdate><volume>103</volume><issue>2</issue><spage>407</spage><epage>415</epage><pages>407-415</pages><issn>0931-2439</issn><eissn>1439-0396</eissn><abstract>Carica papaya is a perennial plant containing bioactive constituents with free radical‐scavenging and immune‐modulating activities. In contrast, the immune suppression is predominant in the periparturient period, where oxidative stress has a substantial impact on the mammary gland health. The aim of the experiment reported here was to determine the potential effect of C. papaya aqueous extract (CPE) on milk production traits, and expression of genes and proteins related to immune and antioxidant status in dairy milk somatic cells (MSCs). Forty Friesian dairy cows were divided equally between a control and CPE‐treated groups (orally drenched 250 µg/kg bwt, once weekly a month before expected parturition and continued until 5 months post‐partum). CPE did not affect milk yield or composition but upregulated the expression of β13‐defensin (DEFB13), cathelicidin 2 (CATHL2), cathelicidin antimicrobial peptide (CATHL3), hepcidin (HAMP), lysozyme (LYZ), catalase (CAT) and glutathione peroxidase (GSH‐Px) in MSCs. The environmental micro‐organisms did not influence the levels of the transcripts. The DEFB13, CATHL2, CATHL3, HAMP and LYZ, but not β1‐defensin (DEFB1) transcripts and proteins were constitutively expressed in MSCs obtained from pathogen‐free udders. It could be concluded that CPE has immunostimulant and antioxidant activities; thereby, it could be utilized to minimize the occurrence of mastitis.</abstract><cop>Germany</cop><pub>Wiley Subscription Services, Inc</pub><pmid>30511402</pmid><doi>10.1111/jpn.13032</doi><tpages>9</tpages><orcidid>https://orcid.org/0000-0003-3915-4640</orcidid></addata></record> |
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| subjects | Adjuvants, Immunologic Animal Feed - analysis Animal Nutritional Physiological Phenomena Animals Antioxidants Antioxidants - metabolism Carica - chemistry Carica papaya Catalase Cattle Cattle - genetics Cattle - immunology Cattle - metabolism Dairy cattle dairy cows Diet - veterinary Dietary Supplements Female Free radicals Gene expression Gene Expression Regulation - drug effects Gene Expression Regulation - immunology Genes Glutathione Glutathione peroxidase Hepcidin immunostimulant Immunostimulants Lysozyme Mammary gland Mammary glands Mastitis Milk Milk - cytology Milk - metabolism Milk production Oxidative stress Parturition Peroxidase Plant Extracts - chemistry Plant Extracts - pharmacology Proteins Scavenging Somatic cells Up-Regulation - drug effects |
| title | Papaya extract upregulates the immune and antioxidants‐related genes, and proteins expression in milk somatic cells of Friesian dairy cows |
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