Lipase‐mediated acidolysis of tristearin with CLA in a packed‐bed reactor: A kinetic study
Solvent‐free acidolysis of tristearin with CLA has been carried out in a packed‐bed reactor. An immobilized lipase from Thermomyces lanuginosa (Lipozyme TL IM) was employed as the biocatalyst. Elevated temperatures (75°C) were utilized to eliminate solid substrates. The reaction kinetics were modele...
Gespeichert in:
| Veröffentlicht in: | Journal of the American Oil Chemists' Society 2002-07, Vol.79 (7), p.655-661 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 661 |
|---|---|
| container_issue | 7 |
| container_start_page | 655 |
| container_title | Journal of the American Oil Chemists' Society |
| container_volume | 79 |
| creator | Torres, Carlos F. Munir, Farida Lessard, Louis P. Hill, Charles G. |
| description | Solvent‐free acidolysis of tristearin with CLA has been carried out in a packed‐bed reactor. An immobilized lipase from Thermomyces lanuginosa (Lipozyme TL IM) was employed as the biocatalyst. Elevated temperatures (75°C) were utilized to eliminate solid substrates. The reaction kinetics were modeled by using a rate equation of the general Michaelis‐Menten form. Both the extent of incorporation of CLA and the extent to which FFA were released were investigated. Positional analysis of the purified TAG obtained after a pseudo space time of 0.6 h indicated that CLA was preferentially incorporated at the sn‐1,3 positions of the glycerol backbone, although 10% of the sn‐2 positions were occupied by CLA residues. At a pseudo space time of 0.6 h, 38% of the initial CLA was incorporated in acylglycerols; the associated extent of hydrolysis was 8.3%. |
| doi_str_mv | 10.1007/s11746-002-0539-x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21497266</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>629054</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3525-1ab2f3e70bd527fc3193242f18aa9ee5d80a8a2bef31da41e9aa2915c7b427163</originalsourceid><addsrcrecordid>eNqFkMtOwzAQRS0EEqXwAey8gV3AY8d5sKsqXlKlLgCJFdbEmQjTtCl2qrY7PoFv5EtICRJLVjMj3XM1OoydgrgAIdLLAJDGSSSEjIRWebTZYwPQOotypWCfDYQQKhISng_ZUQhv3ZkpqQfsZeKWGOjr43NOpcOWSo7WlU29DS7wpuKtd6El9G7B16595ePJiHc78iXaGZUdWHSMJ7Rt46_4iM_cglpneWhX5faYHVRYBzr5nUP2dHP9OL6LJtPb-_FoElmlpY4AC1kpSkVRaplWVkGuZCwryBBzIl1mAjOUBVUKSoyBckSZg7ZpEcsUEjVk533v0jfvKwqtmbtgqa5xQc0qGAlxnspkF4Q-aH0TgqfKLL2bo98aEGZn0vQmTWfS7EyaTcec_ZZjsFhXHhfWhT9QZULEP0-kfW7tatr-X2xG0_GDSLRW36vShnQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21497266</pqid></control><display><type>article</type><title>Lipase‐mediated acidolysis of tristearin with CLA in a packed‐bed reactor: A kinetic study</title><source>Wiley Online Library Journals Frontfile Complete</source><source>SpringerLink Journals - AutoHoldings</source><creator>Torres, Carlos F. ; Munir, Farida ; Lessard, Louis P. ; Hill, Charles G.</creator><creatorcontrib>Torres, Carlos F. ; Munir, Farida ; Lessard, Louis P. ; Hill, Charles G.</creatorcontrib><description>Solvent‐free acidolysis of tristearin with CLA has been carried out in a packed‐bed reactor. An immobilized lipase from Thermomyces lanuginosa (Lipozyme TL IM) was employed as the biocatalyst. Elevated temperatures (75°C) were utilized to eliminate solid substrates. The reaction kinetics were modeled by using a rate equation of the general Michaelis‐Menten form. Both the extent of incorporation of CLA and the extent to which FFA were released were investigated. Positional analysis of the purified TAG obtained after a pseudo space time of 0.6 h indicated that CLA was preferentially incorporated at the sn‐1,3 positions of the glycerol backbone, although 10% of the sn‐2 positions were occupied by CLA residues. At a pseudo space time of 0.6 h, 38% of the initial CLA was incorporated in acylglycerols; the associated extent of hydrolysis was 8.3%.</description><identifier>ISSN: 0003-021X</identifier><identifier>EISSN: 1558-9331</identifier><identifier>DOI: 10.1007/s11746-002-0539-x</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Acidolysis ; Biocatalysts ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; conjugated linoleic acid ; Fat industries ; Food industries ; Fundamental and applied biological sciences. Psychology ; Glycerol ; Hydrolysis ; lipase ; Lipids ; Low temperature effects ; Methods. Procedures. Technologies ; Organic solvents ; packed bed reactor ; Reaction kinetics ; structured lipids ; Substrates ; tristearin</subject><ispartof>Journal of the American Oil Chemists' Society, 2002-07, Vol.79 (7), p.655-661</ispartof><rights>2002 American Oil Chemists' Society (AOCS)</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3525-1ab2f3e70bd527fc3193242f18aa9ee5d80a8a2bef31da41e9aa2915c7b427163</citedby><cites>FETCH-LOGICAL-c3525-1ab2f3e70bd527fc3193242f18aa9ee5d80a8a2bef31da41e9aa2915c7b427163</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1007%2Fs11746-002-0539-x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1007%2Fs11746-002-0539-x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1413,27906,27907,45556,45557</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13800416$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Torres, Carlos F.</creatorcontrib><creatorcontrib>Munir, Farida</creatorcontrib><creatorcontrib>Lessard, Louis P.</creatorcontrib><creatorcontrib>Hill, Charles G.</creatorcontrib><title>Lipase‐mediated acidolysis of tristearin with CLA in a packed‐bed reactor: A kinetic study</title><title>Journal of the American Oil Chemists' Society</title><description>Solvent‐free acidolysis of tristearin with CLA has been carried out in a packed‐bed reactor. An immobilized lipase from Thermomyces lanuginosa (Lipozyme TL IM) was employed as the biocatalyst. Elevated temperatures (75°C) were utilized to eliminate solid substrates. The reaction kinetics were modeled by using a rate equation of the general Michaelis‐Menten form. Both the extent of incorporation of CLA and the extent to which FFA were released were investigated. Positional analysis of the purified TAG obtained after a pseudo space time of 0.6 h indicated that CLA was preferentially incorporated at the sn‐1,3 positions of the glycerol backbone, although 10% of the sn‐2 positions were occupied by CLA residues. At a pseudo space time of 0.6 h, 38% of the initial CLA was incorporated in acylglycerols; the associated extent of hydrolysis was 8.3%.</description><subject>Acidolysis</subject><subject>Biocatalysts</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>conjugated linoleic acid</subject><subject>Fat industries</subject><subject>Food industries</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycerol</subject><subject>Hydrolysis</subject><subject>lipase</subject><subject>Lipids</subject><subject>Low temperature effects</subject><subject>Methods. Procedures. Technologies</subject><subject>Organic solvents</subject><subject>packed bed reactor</subject><subject>Reaction kinetics</subject><subject>structured lipids</subject><subject>Substrates</subject><subject>tristearin</subject><issn>0003-021X</issn><issn>1558-9331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkMtOwzAQRS0EEqXwAey8gV3AY8d5sKsqXlKlLgCJFdbEmQjTtCl2qrY7PoFv5EtICRJLVjMj3XM1OoydgrgAIdLLAJDGSSSEjIRWebTZYwPQOotypWCfDYQQKhISng_ZUQhv3ZkpqQfsZeKWGOjr43NOpcOWSo7WlU29DS7wpuKtd6El9G7B16595ePJiHc78iXaGZUdWHSMJ7Rt46_4iM_cglpneWhX5faYHVRYBzr5nUP2dHP9OL6LJtPb-_FoElmlpY4AC1kpSkVRaplWVkGuZCwryBBzIl1mAjOUBVUKSoyBckSZg7ZpEcsUEjVk533v0jfvKwqtmbtgqa5xQc0qGAlxnspkF4Q-aH0TgqfKLL2bo98aEGZn0vQmTWfS7EyaTcec_ZZjsFhXHhfWhT9QZULEP0-kfW7tatr-X2xG0_GDSLRW36vShnQ</recordid><startdate>200207</startdate><enddate>200207</enddate><creator>Torres, Carlos F.</creator><creator>Munir, Farida</creator><creator>Lessard, Louis P.</creator><creator>Hill, Charles G.</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200207</creationdate><title>Lipase‐mediated acidolysis of tristearin with CLA in a packed‐bed reactor: A kinetic study</title><author>Torres, Carlos F. ; Munir, Farida ; Lessard, Louis P. ; Hill, Charles G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3525-1ab2f3e70bd527fc3193242f18aa9ee5d80a8a2bef31da41e9aa2915c7b427163</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acidolysis</topic><topic>Biocatalysts</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>conjugated linoleic acid</topic><topic>Fat industries</topic><topic>Food industries</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycerol</topic><topic>Hydrolysis</topic><topic>lipase</topic><topic>Lipids</topic><topic>Low temperature effects</topic><topic>Methods. Procedures. Technologies</topic><topic>Organic solvents</topic><topic>packed bed reactor</topic><topic>Reaction kinetics</topic><topic>structured lipids</topic><topic>Substrates</topic><topic>tristearin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torres, Carlos F.</creatorcontrib><creatorcontrib>Munir, Farida</creatorcontrib><creatorcontrib>Lessard, Louis P.</creatorcontrib><creatorcontrib>Hill, Charles G.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of the American Oil Chemists' Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torres, Carlos F.</au><au>Munir, Farida</au><au>Lessard, Louis P.</au><au>Hill, Charles G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lipase‐mediated acidolysis of tristearin with CLA in a packed‐bed reactor: A kinetic study</atitle><jtitle>Journal of the American Oil Chemists' Society</jtitle><date>2002-07</date><risdate>2002</risdate><volume>79</volume><issue>7</issue><spage>655</spage><epage>661</epage><pages>655-661</pages><issn>0003-021X</issn><eissn>1558-9331</eissn><abstract>Solvent‐free acidolysis of tristearin with CLA has been carried out in a packed‐bed reactor. An immobilized lipase from Thermomyces lanuginosa (Lipozyme TL IM) was employed as the biocatalyst. Elevated temperatures (75°C) were utilized to eliminate solid substrates. The reaction kinetics were modeled by using a rate equation of the general Michaelis‐Menten form. Both the extent of incorporation of CLA and the extent to which FFA were released were investigated. Positional analysis of the purified TAG obtained after a pseudo space time of 0.6 h indicated that CLA was preferentially incorporated at the sn‐1,3 positions of the glycerol backbone, although 10% of the sn‐2 positions were occupied by CLA residues. At a pseudo space time of 0.6 h, 38% of the initial CLA was incorporated in acylglycerols; the associated extent of hydrolysis was 8.3%.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s11746-002-0539-x</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-021X |
| ispartof | Journal of the American Oil Chemists' Society, 2002-07, Vol.79 (7), p.655-661 |
| issn | 0003-021X 1558-9331 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21497266 |
| source | Wiley Online Library Journals Frontfile Complete; SpringerLink Journals - AutoHoldings |
| subjects | Acidolysis Biocatalysts Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology conjugated linoleic acid Fat industries Food industries Fundamental and applied biological sciences. Psychology Glycerol Hydrolysis lipase Lipids Low temperature effects Methods. Procedures. Technologies Organic solvents packed bed reactor Reaction kinetics structured lipids Substrates tristearin |
| title | Lipase‐mediated acidolysis of tristearin with CLA in a packed‐bed reactor: A kinetic study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-17T09%3A36%3A31IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Lipase%E2%80%90mediated%20acidolysis%20of%20tristearin%20with%20CLA%20in%20a%20packed%E2%80%90bed%20reactor:%20A%20kinetic%20study&rft.jtitle=Journal%20of%20the%20American%20Oil%20Chemists'%20Society&rft.au=Torres,%20Carlos%20F.&rft.date=2002-07&rft.volume=79&rft.issue=7&rft.spage=655&rft.epage=661&rft.pages=655-661&rft.issn=0003-021X&rft.eissn=1558-9331&rft_id=info:doi/10.1007/s11746-002-0539-x&rft_dat=%3Cproquest_cross%3E629054%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21497266&rft_id=info:pmid/&rfr_iscdi=true |