Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina
The purple (Sulphur) phototrophic bacterium, Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T. roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The struc...
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| Veröffentlicht in: | International journal of hydrogen energy 2002-11, Vol.27 (11), p.1463-1469 |
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| container_title | International journal of hydrogen energy |
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| creator | Kovács, Kornél L. Fodor, Barna Kovács, Ákos T. Csanádi, Gyula Maróti, Gergely Balogh, Judit Arvani, Solmaz Rákhely, Gábor |
| description | The purple (Sulphur) phototrophic bacterium,
Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T.
roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster
hoxEFUYH was identified and sequenced. In addition, genes homologous to
hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.
Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for
T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the
hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to
hypF, the following accessory genes were identified:
hydD,
hupK,
hypC1,
hypC2,
hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress. |
| doi_str_mv | 10.1016/S0360-3199(02)00097-6 |
| format | Article |
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Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T.
roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster
hoxEFUYH was identified and sequenced. In addition, genes homologous to
hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.
Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for
T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the
hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to
hypF, the following accessory genes were identified:
hydD,
hupK,
hypC1,
hypC2,
hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress.</description><identifier>ISSN: 0360-3199</identifier><identifier>EISSN: 1879-3487</identifier><identifier>DOI: 10.1016/S0360-3199(02)00097-6</identifier><identifier>CODEN: IJHEDX</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>[NiFe] hydrogenase ; Accessory genes ; Analytical, structural and metabolic biochemistry ; Bacteria ; Biohydrogen production ; Biological and medical sciences ; Biosynthesis ; Catalyst activity ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genes ; Hydrogen ; Mutagenesis ; Oxidoreductases ; Photosynthesis ; Photosynthetic bacteria ; Thiocapsa roseopersicina</subject><ispartof>International journal of hydrogen energy, 2002-11, Vol.27 (11), p.1463-1469</ispartof><rights>2002 International Association for Hydrogen Energy</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0360-3199(02)00097-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13968017$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Kovács, Kornél L.</creatorcontrib><creatorcontrib>Fodor, Barna</creatorcontrib><creatorcontrib>Kovács, Ákos T.</creatorcontrib><creatorcontrib>Csanádi, Gyula</creatorcontrib><creatorcontrib>Maróti, Gergely</creatorcontrib><creatorcontrib>Balogh, Judit</creatorcontrib><creatorcontrib>Arvani, Solmaz</creatorcontrib><creatorcontrib>Rákhely, Gábor</creatorcontrib><title>Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina</title><title>International journal of hydrogen energy</title><description>The purple (Sulphur) phototrophic bacterium,
Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T.
roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster
hoxEFUYH was identified and sequenced. In addition, genes homologous to
hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.
Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for
T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the
hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to
hypF, the following accessory genes were identified:
hydD,
hupK,
hypC1,
hypC2,
hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress.</description><subject>[NiFe] hydrogenase</subject><subject>Accessory genes</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Bacteria</subject><subject>Biohydrogen production</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Catalyst activity</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genes</subject><subject>Hydrogen</subject><subject>Mutagenesis</subject><subject>Oxidoreductases</subject><subject>Photosynthesis</subject><subject>Photosynthetic bacteria</subject><subject>Thiocapsa roseopersicina</subject><issn>0360-3199</issn><issn>1879-3487</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNpFkV1LHTEQhkNR6PHjJwi5aVHotslmNx9XIlJrQdoL9UokzElmPSlrcprZUzj_vusHejUwPDPMvA9jR1J8lULqb9dCadEo6dyxaE-EEM40-gNbSGtcozprdtjiDfnI9oj-CCGN6NyClcttrOUBMxDSFw4hIFGpWz63kDjkyKcV8ooPmxGmVDIvA7_7lS7wnq_eR_kyFdrmGaVEPGV-s0olwJqA10JY1lgphZThgO0OMBIevtZ9dnvx_eb8srn6_ePn-dlVg60RU9NHa7RcBlj2tpOtc8pq0K1c9qCxg-CiaHU_qN7FXis1oOisjSYaqdr5Vav22eeXveta_m6QJv-YKOA4QsayId_Kzhkp-xn89AoCBRiHCjkk8uuaHqFuvVRO2zmsmTt94XC--l_C6ikkzAFjqhgmH0vyUvgnIf5ZiH9K24vWPwvxWv0H2Ex_tQ</recordid><startdate>20021101</startdate><enddate>20021101</enddate><creator>Kovács, Kornél L.</creator><creator>Fodor, Barna</creator><creator>Kovács, Ákos T.</creator><creator>Csanádi, Gyula</creator><creator>Maróti, Gergely</creator><creator>Balogh, Judit</creator><creator>Arvani, Solmaz</creator><creator>Rákhely, Gábor</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope></search><sort><creationdate>20021101</creationdate><title>Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina</title><author>Kovács, Kornél L. ; Fodor, Barna ; Kovács, Ákos T. ; Csanádi, Gyula ; Maróti, Gergely ; Balogh, Judit ; Arvani, Solmaz ; Rákhely, Gábor</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e270t-5d8761bcab5841299386a621b5a6e4ac9d0265f359d5633fe0488d7d713203683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>[NiFe] hydrogenase</topic><topic>Accessory genes</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Bacteria</topic><topic>Biohydrogen production</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Catalyst activity</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genes</topic><topic>Hydrogen</topic><topic>Mutagenesis</topic><topic>Oxidoreductases</topic><topic>Photosynthesis</topic><topic>Photosynthetic bacteria</topic><topic>Thiocapsa roseopersicina</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kovács, Kornél L.</creatorcontrib><creatorcontrib>Fodor, Barna</creatorcontrib><creatorcontrib>Kovács, Ákos T.</creatorcontrib><creatorcontrib>Csanádi, Gyula</creatorcontrib><creatorcontrib>Maróti, Gergely</creatorcontrib><creatorcontrib>Balogh, Judit</creatorcontrib><creatorcontrib>Arvani, Solmaz</creatorcontrib><creatorcontrib>Rákhely, Gábor</creatorcontrib><collection>Pascal-Francis</collection><jtitle>International journal of hydrogen energy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kovács, Kornél L.</au><au>Fodor, Barna</au><au>Kovács, Ákos T.</au><au>Csanádi, Gyula</au><au>Maróti, Gergely</au><au>Balogh, Judit</au><au>Arvani, Solmaz</au><au>Rákhely, Gábor</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina</atitle><jtitle>International journal of hydrogen energy</jtitle><date>2002-11-01</date><risdate>2002</risdate><volume>27</volume><issue>11</issue><spage>1463</spage><epage>1469</epage><pages>1463-1469</pages><issn>0360-3199</issn><eissn>1879-3487</eissn><coden>IJHEDX</coden><abstract>The purple (Sulphur) phototrophic bacterium,
Thiocapsa roseopersicina BBS contains several [NiFe] hydrogenases, of which two are membrane bound. Mutant T.
roseopersicina cells, carrying deletions in both gene clusters showed hydrogenase activity. This activity was located in the cytoplasm. The structural gene cluster
hoxEFUYH was identified and sequenced. In addition, genes homologous to
hupUV/hoxBC, the hydrogen sensing hydrogenase have been identified and sequenced.
Regulation of hydrogenase biosynthesis was studied in detail for HydSL (renamed HynSL). A random mutagenesis system was optimised for
T. roseopersicina. One of the mutations was in a gene similar to that coding for the HypF proteins in other organisms. Inactivation of the
hypF gene resulted in a 60-fold increase in hydrogen evolution under nitrogen fixing conditions. In addition to
hypF, the following accessory genes were identified:
hydD,
hupK,
hypC1,
hypC2,
hypDE. Characterisation of the corresponding gene products and search for additional accessory genes are in progress.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><doi>10.1016/S0360-3199(02)00097-6</doi><tpages>7</tpages></addata></record> |
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| subjects | [NiFe] hydrogenase Accessory genes Analytical, structural and metabolic biochemistry Bacteria Biohydrogen production Biological and medical sciences Biosynthesis Catalyst activity Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Gene expression Genes Hydrogen Mutagenesis Oxidoreductases Photosynthesis Photosynthetic bacteria Thiocapsa roseopersicina |
| title | Hydrogenases, accessory genes and the regulation of [NiFe] hydrogenase biosynthesis in Thiocapsa roseopersicina |
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