Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice
The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. Phenotypic analysis of an isogenic mntH mutant indicates that MntH is the sole high-affinity manganese transporter in this bacterium but that MntH does not play a detecta...
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| Veröffentlicht in: | Infection and Immunity 2009-08, Vol.77 (8), p.3466-3474 |
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| creator | Anderson, Eric S Paulley, James T Gaines, Jennifer M Valderas, Michelle W Martin, Daniel W Menscher, Evan Brown, Timothy D Burns, Colin S Roop, R. Martin II |
| description | The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. Phenotypic analysis of an isogenic mntH mutant indicates that MntH is the sole high-affinity manganese transporter in this bacterium but that MntH does not play a detectable role in the transport of Fe²⁺, Zn²⁺, Co²⁺, or Ni²⁺. Consistent with the apparent selectivity of the corresponding gene product, the expression of the mntH gene in B. abortus 2308 is repressed by Mn²⁺, but not Fe²⁺, and this Mn-responsive expression is mediated by a Mur-like repressor. The B. abortus mntH mutant MWV15 exhibits increased susceptibility to oxidative killing in vitro compared to strain 2308, and a comparative analysis of the superoxide dismutase activities present in these two strains indicates that the parental strain requires MntH in order to make wild-type levels of its manganese superoxide dismutase SodA. The B. abortus mntH mutant also exhibits extreme attenuation in both cultured murine macrophages and experimentally infected C57BL/6 mice. These experimental findings indicate that Mn²⁺ transport mediated by MntH plays an important role in the physiology of B. abortus 2308, particularly during its intracellular survival and replication in the host. |
| doi_str_mv | 10.1128/IAI.00444-09 |
| format | Article |
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Martin II</creator><creatorcontrib>Anderson, Eric S ; Paulley, James T ; Gaines, Jennifer M ; Valderas, Michelle W ; Martin, Daniel W ; Menscher, Evan ; Brown, Timothy D ; Burns, Colin S ; Roop, R. Martin II</creatorcontrib><description>The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. Phenotypic analysis of an isogenic mntH mutant indicates that MntH is the sole high-affinity manganese transporter in this bacterium but that MntH does not play a detectable role in the transport of Fe²⁺, Zn²⁺, Co²⁺, or Ni²⁺. Consistent with the apparent selectivity of the corresponding gene product, the expression of the mntH gene in B. abortus 2308 is repressed by Mn²⁺, but not Fe²⁺, and this Mn-responsive expression is mediated by a Mur-like repressor. The B. abortus mntH mutant MWV15 exhibits increased susceptibility to oxidative killing in vitro compared to strain 2308, and a comparative analysis of the superoxide dismutase activities present in these two strains indicates that the parental strain requires MntH in order to make wild-type levels of its manganese superoxide dismutase SodA. The B. abortus mntH mutant also exhibits extreme attenuation in both cultured murine macrophages and experimentally infected C57BL/6 mice. These experimental findings indicate that Mn²⁺ transport mediated by MntH plays an important role in the physiology of B. abortus 2308, particularly during its intracellular survival and replication in the host.</description><identifier>ISSN: 0019-9567</identifier><identifier>EISSN: 1098-5522</identifier><identifier>DOI: 10.1128/IAI.00444-09</identifier><identifier>PMID: 19487482</identifier><identifier>CODEN: INFIBR</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacterial Infections ; Bacterial Proteins - physiology ; Bacteriology ; Biological and medical sciences ; Brucella abortus ; Brucella abortus - pathogenicity ; Brucellosis - microbiology ; Cation Transport Proteins - deficiency ; Cation Transport Proteins - physiology ; Cells, Cultured ; Colony Count, Microbial ; Cytoplasm - microbiology ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Deletion ; Gene Expression Profiling ; Gene Expression Regulation, Bacterial ; Genetic Complementation Test ; Genomes ; Iron ; Macrophages ; Macrophages, Peritoneal - immunology ; Macrophages, Peritoneal - microbiology ; Manganese ; Manganese - metabolism ; Mice ; Mice, Inbred C57BL ; Microbiology ; Miscellaneous ; Nucleotide sequence ; Replication ; Repressors ; Spleen - microbiology ; Superoxide dismutase ; Virulence ; Virulence Factors - deficiency ; Virulence Factors - physiology ; Zinc</subject><ispartof>Infection and Immunity, 2009-08, Vol.77 (8), p.3466-3474</ispartof><rights>2009 INIST-CNRS</rights><rights>Copyright © 2009, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c536t-290a3e49b6538dc0dedf1e8456aafd67d64227f4958a14c4f5e471dd366bc7ed3</citedby><cites>FETCH-LOGICAL-c536t-290a3e49b6538dc0dedf1e8456aafd67d64227f4958a14c4f5e471dd366bc7ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715675/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2715675/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,315,729,782,786,887,3190,3191,27931,27932,53798,53800</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21781082$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19487482$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Anderson, Eric S</creatorcontrib><creatorcontrib>Paulley, James T</creatorcontrib><creatorcontrib>Gaines, Jennifer M</creatorcontrib><creatorcontrib>Valderas, Michelle W</creatorcontrib><creatorcontrib>Martin, Daniel W</creatorcontrib><creatorcontrib>Menscher, Evan</creatorcontrib><creatorcontrib>Brown, Timothy D</creatorcontrib><creatorcontrib>Burns, Colin S</creatorcontrib><creatorcontrib>Roop, R. Martin II</creatorcontrib><title>Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice</title><title>Infection and Immunity</title><addtitle>Infect Immun</addtitle><description>The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. Phenotypic analysis of an isogenic mntH mutant indicates that MntH is the sole high-affinity manganese transporter in this bacterium but that MntH does not play a detectable role in the transport of Fe²⁺, Zn²⁺, Co²⁺, or Ni²⁺. Consistent with the apparent selectivity of the corresponding gene product, the expression of the mntH gene in B. abortus 2308 is repressed by Mn²⁺, but not Fe²⁺, and this Mn-responsive expression is mediated by a Mur-like repressor. The B. abortus mntH mutant MWV15 exhibits increased susceptibility to oxidative killing in vitro compared to strain 2308, and a comparative analysis of the superoxide dismutase activities present in these two strains indicates that the parental strain requires MntH in order to make wild-type levels of its manganese superoxide dismutase SodA. The B. abortus mntH mutant also exhibits extreme attenuation in both cultured murine macrophages and experimentally infected C57BL/6 mice. These experimental findings indicate that Mn²⁺ transport mediated by MntH plays an important role in the physiology of B. abortus 2308, particularly during its intracellular survival and replication in the host.</description><subject>Animals</subject><subject>Bacterial Infections</subject><subject>Bacterial Proteins - physiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Brucella abortus</subject><subject>Brucella abortus - pathogenicity</subject><subject>Brucellosis - microbiology</subject><subject>Cation Transport Proteins - deficiency</subject><subject>Cation Transport Proteins - physiology</subject><subject>Cells, Cultured</subject><subject>Colony Count, Microbial</subject><subject>Cytoplasm - microbiology</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetic Complementation Test</subject><subject>Genomes</subject><subject>Iron</subject><subject>Macrophages</subject><subject>Macrophages, Peritoneal - immunology</subject><subject>Macrophages, Peritoneal - microbiology</subject><subject>Manganese</subject><subject>Manganese - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Nucleotide sequence</subject><subject>Replication</subject><subject>Repressors</subject><subject>Spleen - microbiology</subject><subject>Superoxide dismutase</subject><subject>Virulence</subject><subject>Virulence Factors - deficiency</subject><subject>Virulence Factors - physiology</subject><subject>Zinc</subject><issn>0019-9567</issn><issn>1098-5522</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkc1v0zAYhyMEYt3gxhnMYZzIsB1_5YI0ymCVVnFg42q9dd60RonT2Qls_z1eWw04WZYf_96PpyheMXrGGDcfFueLM0qFECWtnxQzRmtTSsn502JGKavLWip9VByn9DNfM2aeF0esFkYLw2fF3RLCGgImJNcRQtoOccRIlmG8JItEgMyjH72DjvzwceowOCSfMSO9DxBG0g6RfIqTw64DAqv8e0qEV9QQH8jF3Raj7zGM0HX3ZBFadCM2ZOkdviietdAlfHk4T4qbLxfX88vy6tvXxfz8qnSyUmPJawoVinqlZGUaRxtsWoZGSAXQNko3SnCuW1FLA0w40UoUmjVNpdTKaWyqk-LjPnc7rXpsXG4mQme3uS-I93YAb_9_CX5j18MvyzXLm5M54N0hIA63E6bR9j7t5g04TMlylovXos7g-z3o4pBSxPaxCKP2QZXNquxOlaUP-Ot_G_sLH9xk4PQAQMoC2qzH-fTIcaYNozvu7Z7b-PXmt49oIfXW58G0tsZWQqnMvNkzLQwW1jHn3HznlFWUKSmlNtUfED6x_w</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Anderson, Eric S</creator><creator>Paulley, James T</creator><creator>Gaines, Jennifer M</creator><creator>Valderas, Michelle W</creator><creator>Martin, Daniel W</creator><creator>Menscher, Evan</creator><creator>Brown, Timothy D</creator><creator>Burns, Colin S</creator><creator>Roop, R. Martin II</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>5PM</scope></search><sort><creationdate>20090801</creationdate><title>Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice</title><author>Anderson, Eric S ; Paulley, James T ; Gaines, Jennifer M ; Valderas, Michelle W ; Martin, Daniel W ; Menscher, Evan ; Brown, Timothy D ; Burns, Colin S ; Roop, R. 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Psychology</topic><topic>Gene Deletion</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetic Complementation Test</topic><topic>Genomes</topic><topic>Iron</topic><topic>Macrophages</topic><topic>Macrophages, Peritoneal - immunology</topic><topic>Macrophages, Peritoneal - microbiology</topic><topic>Manganese</topic><topic>Manganese - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Nucleotide sequence</topic><topic>Replication</topic><topic>Repressors</topic><topic>Spleen - microbiology</topic><topic>Superoxide dismutase</topic><topic>Virulence</topic><topic>Virulence Factors - deficiency</topic><topic>Virulence Factors - physiology</topic><topic>Zinc</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Anderson, Eric S</creatorcontrib><creatorcontrib>Paulley, James T</creatorcontrib><creatorcontrib>Gaines, Jennifer M</creatorcontrib><creatorcontrib>Valderas, Michelle W</creatorcontrib><creatorcontrib>Martin, Daniel W</creatorcontrib><creatorcontrib>Menscher, Evan</creatorcontrib><creatorcontrib>Brown, Timothy D</creatorcontrib><creatorcontrib>Burns, Colin S</creatorcontrib><creatorcontrib>Roop, R. 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Martin II</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice</atitle><jtitle>Infection and Immunity</jtitle><addtitle>Infect Immun</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>77</volume><issue>8</issue><spage>3466</spage><epage>3474</epage><pages>3466-3474</pages><issn>0019-9567</issn><eissn>1098-5522</eissn><coden>INFIBR</coden><abstract>The gene designated BAB1_1460 in the Brucella abortus 2308 genome sequence is predicted to encode the manganese transporter MntH. Phenotypic analysis of an isogenic mntH mutant indicates that MntH is the sole high-affinity manganese transporter in this bacterium but that MntH does not play a detectable role in the transport of Fe²⁺, Zn²⁺, Co²⁺, or Ni²⁺. Consistent with the apparent selectivity of the corresponding gene product, the expression of the mntH gene in B. abortus 2308 is repressed by Mn²⁺, but not Fe²⁺, and this Mn-responsive expression is mediated by a Mur-like repressor. The B. abortus mntH mutant MWV15 exhibits increased susceptibility to oxidative killing in vitro compared to strain 2308, and a comparative analysis of the superoxide dismutase activities present in these two strains indicates that the parental strain requires MntH in order to make wild-type levels of its manganese superoxide dismutase SodA. The B. abortus mntH mutant also exhibits extreme attenuation in both cultured murine macrophages and experimentally infected C57BL/6 mice. These experimental findings indicate that Mn²⁺ transport mediated by MntH plays an important role in the physiology of B. abortus 2308, particularly during its intracellular survival and replication in the host.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>19487482</pmid><doi>10.1128/IAI.00444-09</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Bacterial Infections Bacterial Proteins - physiology Bacteriology Biological and medical sciences Brucella abortus Brucella abortus - pathogenicity Brucellosis - microbiology Cation Transport Proteins - deficiency Cation Transport Proteins - physiology Cells, Cultured Colony Count, Microbial Cytoplasm - microbiology Female Fundamental and applied biological sciences. Psychology Gene Deletion Gene Expression Profiling Gene Expression Regulation, Bacterial Genetic Complementation Test Genomes Iron Macrophages Macrophages, Peritoneal - immunology Macrophages, Peritoneal - microbiology Manganese Manganese - metabolism Mice Mice, Inbred C57BL Microbiology Miscellaneous Nucleotide sequence Replication Repressors Spleen - microbiology Superoxide dismutase Virulence Virulence Factors - deficiency Virulence Factors - physiology Zinc |
| title | Manganese Transporter MntH Is a Critical Virulence Determinant for Brucella abortus 2308 in Experimentally Infected Mice |
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