Magnesium concentration effects on cruciform extrusion in supercoiled DNA examined by atomic force microscopy
Cruciform structures can form in DNA sequences with inverted repeats or palindromic symmetry under unwinding torsional stress. DNA repeats are widespread in the genomes of eukaryotes and prokaryotes, and their extrusion into cruciform structures may be involved in various genetic processes, includin...
Gespeichert in:
| Veröffentlicht in: | Applied surface science 2002-03, Vol.188 (3), p.481-485 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 485 |
|---|---|
| container_issue | 3 |
| container_start_page | 481 |
| container_title | Applied surface science |
| container_volume | 188 |
| creator | Chasovskikh, S. Dritschilo, A. |
| description | Cruciform structures can form in DNA sequences with inverted repeats or palindromic symmetry under unwinding torsional stress. DNA repeats are widespread in the genomes of eukaryotes and prokaryotes, and their extrusion into cruciform structures may be involved in various genetic processes, including transcription and replication. Intracellular Mg
2+ may change the free energy of supercoiling to provide the driving force for cruciform extrusion. We have used atomic force microscopy (AFM) to image the effects of magnesium concentrations on cruciform extrusions in supercoiled DNA. Mg
2+ concentration dependent cruciform extrusions were observed in the supercoiled topoisomer of the pPR-PARP plasmid (〈
σ〉=−0.065). These data show an application of AFM imaging to demonstrate that the presence of Mg
2+ results in increased extrusions and promotes compact cruciform conformation. |
| doi_str_mv | 10.1016/S0169-4332(01)00970-9 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_21495180</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0169433201009709</els_id><sourcerecordid>613267</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-6eadc37684a5f214f80898e0cbf50af260d908110a9ac8f75dcf8a22386ae8d3</originalsourceid><addsrcrecordid>eNqFkE9v1DAQxS0EEkvhIyD50goOgXGcP_apqgq0SIUe2rs1nYwrV0m8tRPEfnu83QqOXOwZvfdm7J8Q7xV8UqC6zzflsFWjdf0B1EcA20NlX4iNMr2u2tY0L8Xmr-W1eJPzA4Cqi7oR0w-8nzmHdZIUZ-J5SbiEOEv2nmnJspSUVgo-pkny7yWteS-HWeZ1y4liGHmQX36eFRGnMJfmbidxiVMgWULEslQpZorb3VvxyuOY-d3zfSRuv329Pb-srq4vvp-fXVXU6GapOsaBdN-ZBltfq8YbMNYw0J1vAX3dwWDBKAVokYzv24G8wbrWpkM2gz4SJ4ex2xQfV86Lm0ImHkecOa7ZlZG2VQaKsT0Y9w_Mib3bpjBh2jkFbs_WPbF1e3AOlHti62zJHT8vwEw4-oQzhfwvrDvTW90X3-nBx-WzvwInlylwwTyEVOi6IYb_bPoDrB6QMg</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21495180</pqid></control><display><type>article</type><title>Magnesium concentration effects on cruciform extrusion in supercoiled DNA examined by atomic force microscopy</title><source>Access via ScienceDirect (Elsevier)</source><creator>Chasovskikh, S. ; Dritschilo, A.</creator><creatorcontrib>Chasovskikh, S. ; Dritschilo, A.</creatorcontrib><description>Cruciform structures can form in DNA sequences with inverted repeats or palindromic symmetry under unwinding torsional stress. DNA repeats are widespread in the genomes of eukaryotes and prokaryotes, and their extrusion into cruciform structures may be involved in various genetic processes, including transcription and replication. Intracellular Mg
2+ may change the free energy of supercoiling to provide the driving force for cruciform extrusion. We have used atomic force microscopy (AFM) to image the effects of magnesium concentrations on cruciform extrusions in supercoiled DNA. Mg
2+ concentration dependent cruciform extrusions were observed in the supercoiled topoisomer of the pPR-PARP plasmid (〈
σ〉=−0.065). These data show an application of AFM imaging to demonstrate that the presence of Mg
2+ results in increased extrusions and promotes compact cruciform conformation.</description><identifier>ISSN: 0169-4332</identifier><identifier>EISSN: 1873-5584</identifier><identifier>DOI: 10.1016/S0169-4332(01)00970-9</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analytical, structural and metabolic biochemistry ; Atomic force microscopy ; Biological and medical sciences ; Cruciform ; DNA ; DNA sequences ; Dna, deoxyribonucleoproteins ; Extrusion ; Free energy ; Fundamental and applied biological sciences. Psychology ; Imaging techniques ; Magnesium ; Magnesium printing plates ; Nucleic acids ; Torsional stress</subject><ispartof>Applied surface science, 2002-03, Vol.188 (3), p.481-485</ispartof><rights>2002 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-6eadc37684a5f214f80898e0cbf50af260d908110a9ac8f75dcf8a22386ae8d3</citedby><cites>FETCH-LOGICAL-c434t-6eadc37684a5f214f80898e0cbf50af260d908110a9ac8f75dcf8a22386ae8d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0169-4332(01)00970-9$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=13687937$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Chasovskikh, S.</creatorcontrib><creatorcontrib>Dritschilo, A.</creatorcontrib><title>Magnesium concentration effects on cruciform extrusion in supercoiled DNA examined by atomic force microscopy</title><title>Applied surface science</title><description>Cruciform structures can form in DNA sequences with inverted repeats or palindromic symmetry under unwinding torsional stress. DNA repeats are widespread in the genomes of eukaryotes and prokaryotes, and their extrusion into cruciform structures may be involved in various genetic processes, including transcription and replication. Intracellular Mg
2+ may change the free energy of supercoiling to provide the driving force for cruciform extrusion. We have used atomic force microscopy (AFM) to image the effects of magnesium concentrations on cruciform extrusions in supercoiled DNA. Mg
2+ concentration dependent cruciform extrusions were observed in the supercoiled topoisomer of the pPR-PARP plasmid (〈
σ〉=−0.065). These data show an application of AFM imaging to demonstrate that the presence of Mg
2+ results in increased extrusions and promotes compact cruciform conformation.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Atomic force microscopy</subject><subject>Biological and medical sciences</subject><subject>Cruciform</subject><subject>DNA</subject><subject>DNA sequences</subject><subject>Dna, deoxyribonucleoproteins</subject><subject>Extrusion</subject><subject>Free energy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Imaging techniques</subject><subject>Magnesium</subject><subject>Magnesium printing plates</subject><subject>Nucleic acids</subject><subject>Torsional stress</subject><issn>0169-4332</issn><issn>1873-5584</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkE9v1DAQxS0EEkvhIyD50goOgXGcP_apqgq0SIUe2rs1nYwrV0m8tRPEfnu83QqOXOwZvfdm7J8Q7xV8UqC6zzflsFWjdf0B1EcA20NlX4iNMr2u2tY0L8Xmr-W1eJPzA4Cqi7oR0w-8nzmHdZIUZ-J5SbiEOEv2nmnJspSUVgo-pkny7yWteS-HWeZ1y4liGHmQX36eFRGnMJfmbidxiVMgWULEslQpZorb3VvxyuOY-d3zfSRuv329Pb-srq4vvp-fXVXU6GapOsaBdN-ZBltfq8YbMNYw0J1vAX3dwWDBKAVokYzv24G8wbrWpkM2gz4SJ4ex2xQfV86Lm0ImHkecOa7ZlZG2VQaKsT0Y9w_Mib3bpjBh2jkFbs_WPbF1e3AOlHti62zJHT8vwEw4-oQzhfwvrDvTW90X3-nBx-WzvwInlylwwTyEVOi6IYb_bPoDrB6QMg</recordid><startdate>20020328</startdate><enddate>20020328</enddate><creator>Chasovskikh, S.</creator><creator>Dritschilo, A.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020328</creationdate><title>Magnesium concentration effects on cruciform extrusion in supercoiled DNA examined by atomic force microscopy</title><author>Chasovskikh, S. ; Dritschilo, A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-6eadc37684a5f214f80898e0cbf50af260d908110a9ac8f75dcf8a22386ae8d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>Atomic force microscopy</topic><topic>Biological and medical sciences</topic><topic>Cruciform</topic><topic>DNA</topic><topic>DNA sequences</topic><topic>Dna, deoxyribonucleoproteins</topic><topic>Extrusion</topic><topic>Free energy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Imaging techniques</topic><topic>Magnesium</topic><topic>Magnesium printing plates</topic><topic>Nucleic acids</topic><topic>Torsional stress</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chasovskikh, S.</creatorcontrib><creatorcontrib>Dritschilo, A.</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Applied surface science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chasovskikh, S.</au><au>Dritschilo, A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Magnesium concentration effects on cruciform extrusion in supercoiled DNA examined by atomic force microscopy</atitle><jtitle>Applied surface science</jtitle><date>2002-03-28</date><risdate>2002</risdate><volume>188</volume><issue>3</issue><spage>481</spage><epage>485</epage><pages>481-485</pages><issn>0169-4332</issn><eissn>1873-5584</eissn><abstract>Cruciform structures can form in DNA sequences with inverted repeats or palindromic symmetry under unwinding torsional stress. DNA repeats are widespread in the genomes of eukaryotes and prokaryotes, and their extrusion into cruciform structures may be involved in various genetic processes, including transcription and replication. Intracellular Mg
2+ may change the free energy of supercoiling to provide the driving force for cruciform extrusion. We have used atomic force microscopy (AFM) to image the effects of magnesium concentrations on cruciform extrusions in supercoiled DNA. Mg
2+ concentration dependent cruciform extrusions were observed in the supercoiled topoisomer of the pPR-PARP plasmid (〈
σ〉=−0.065). These data show an application of AFM imaging to demonstrate that the presence of Mg
2+ results in increased extrusions and promotes compact cruciform conformation.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0169-4332(01)00970-9</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0169-4332 |
| ispartof | Applied surface science, 2002-03, Vol.188 (3), p.481-485 |
| issn | 0169-4332 1873-5584 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21495180 |
| source | Access via ScienceDirect (Elsevier) |
| subjects | Analytical, structural and metabolic biochemistry Atomic force microscopy Biological and medical sciences Cruciform DNA DNA sequences Dna, deoxyribonucleoproteins Extrusion Free energy Fundamental and applied biological sciences. Psychology Imaging techniques Magnesium Magnesium printing plates Nucleic acids Torsional stress |
| title | Magnesium concentration effects on cruciform extrusion in supercoiled DNA examined by atomic force microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-21T07%3A03%3A47IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Magnesium%20concentration%20effects%20on%20cruciform%20extrusion%20in%20supercoiled%20DNA%20examined%20by%20atomic%20force%20microscopy&rft.jtitle=Applied%20surface%20science&rft.au=Chasovskikh,%20S.&rft.date=2002-03-28&rft.volume=188&rft.issue=3&rft.spage=481&rft.epage=485&rft.pages=481-485&rft.issn=0169-4332&rft.eissn=1873-5584&rft_id=info:doi/10.1016/S0169-4332(01)00970-9&rft_dat=%3Cproquest_cross%3E613267%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21495180&rft_id=info:pmid/&rft_els_id=S0169433201009709&rfr_iscdi=true |