Piglet weight gain during the first two weeks of lactation influences the immune system development
•Piglets’ immune system development is affected by birth weight and early growth performances.•Expression of genes involved in defense response and oxidative stress was modulated in LWG piglets.•Intestinal antigen presenting cells markers (APC) were decreased in LWG piglets.•The increase of B cells...
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| Veröffentlicht in: | Veterinary immunology and immunopathology 2018-12, Vol.206, p.25-34 |
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| creator | Lessard, Martin Blais, Mylène Beaudoin, Frédéric Deschene, Karine Verso, Luca Lo Bissonnette, Nathalie Lauzon, Karoline Guay, Frédéric |
| description | •Piglets’ immune system development is affected by birth weight and early growth performances.•Expression of genes involved in defense response and oxidative stress was modulated in LWG piglets.•Intestinal antigen presenting cells markers (APC) were decreased in LWG piglets.•The increase of B cells in HWG piglets was not observed in LWG piglets.•The regulation of various biological processes supporting neonatal development is associated to maternal lactocrine signaling.
The aim of this study was to investigate the effect of the piglet growth during the first week of life on ileal expression of genes and on development of the immune system. Eight litters adjusted to 12 piglets were used. Within each litter, the piglet that showed the lowest weight gain (LWG; n = 8) and the one that showed the highest weight gain (HWG; n = 8) in their first week of life were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated on days 8 and 16 to characterize cellular population profiles and to assess ex-vivo secretion of interleukin-10 (IL-10), IL-6 and tumor necrosis factor-α (TNF-α). On day 16, piglets were euthanized and ileum samples were collected to extract RNA for microarray analysis and gene expression by qPCR. As expected, growth performance of LWG piglet was impaired compared to HWG piglets (P |
| doi_str_mv | 10.1016/j.vetimm.2018.11.005 |
| format | Article |
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The aim of this study was to investigate the effect of the piglet growth during the first week of life on ileal expression of genes and on development of the immune system. Eight litters adjusted to 12 piglets were used. Within each litter, the piglet that showed the lowest weight gain (LWG; n = 8) and the one that showed the highest weight gain (HWG; n = 8) in their first week of life were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated on days 8 and 16 to characterize cellular population profiles and to assess ex-vivo secretion of interleukin-10 (IL-10), IL-6 and tumor necrosis factor-α (TNF-α). On day 16, piglets were euthanized and ileum samples were collected to extract RNA for microarray analysis and gene expression by qPCR. As expected, growth performance of LWG piglet was impaired compared to HWG piglets (P < 0.05). From day 8 to 16, the percentage of CD21+ B cells significantly increased in blood of heavier HWG piglets while the percentage remained constant in smaller LWG piglets (P weight x day = 0.01). For the CD4+CD8α− Th cells, a marked increase was observed in LWG piglets from 8 to 16 days of age (P = 0.002) whereas no significant change occurred in HWG piglets. Percentages of CD14+ monocytes and other MHC-II+ cells were respectively higher and lower on day 8 compared to day 16 for both groups of piglets (P < 0.01). On day 8, LPS-activated PBMC from LWG piglets produced less IL-6 compared to HWG piglets (P < 0.05). Microarray analysis of gene expression in piglets’ ileum tissue indicated that several genes involed in defense response and response to oxidative stress were modulated differently in LWG compared to HWG. Gene analysis by Q-PCR confirmed microarray results and revealed that IL-10, SOD1, NOS2, NOD2, TLR4, TLR9, CD40 and CD74 expressions were significantly decreased (P < 0.05) in LWG in comparison to HWG piglets, while MYD88 and NFkBiA showed a tendency to decrease (0.05 ≤ P < 0.07). These results suggest that birth weight and milk intake affect the growth performances and the development of immunity by modulating the expression of genes associated with immunity and oxidative stress in piglets’ intestinal tissue, and by affecting the leukocyte populations involved in innate and cell-mediated immunity in nursing piglets. Therefore, impaired development of immune system in LWG piglets might have an impact on their resistance to infections later in life.</description><identifier>ISSN: 0165-2427</identifier><identifier>EISSN: 1873-2534</identifier><identifier>DOI: 10.1016/j.vetimm.2018.11.005</identifier><identifier>PMID: 30502909</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Cytokines - blood ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; Gene Expression ; Ileum - anatomy & histology ; Ileum - growth & development ; Ileum - immunology ; Immune System - growth & development ; Immune system development ; Lactation ; Leukocytes, Mononuclear - immunology ; Microarray Analysis - veterinary ; Piglet ; Real-Time Polymerase Chain Reaction - veterinary ; Swine - growth & development ; Swine - immunology ; Weight gain ; Weight Gain - immunology</subject><ispartof>Veterinary immunology and immunopathology, 2018-12, Vol.206, p.25-34</ispartof><rights>2018</rights><rights>Copyright © 2018. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c362t-bc5b11d144b520dd969e67b4cdc553d29668b921cddc38b1eb3cabbee0605ad73</citedby><cites>FETCH-LOGICAL-c362t-bc5b11d144b520dd969e67b4cdc553d29668b921cddc38b1eb3cabbee0605ad73</cites><orcidid>0000-0001-6964-1293 ; 0000-0002-5771-566X ; 0000-0003-3281-209X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0165242717305147$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/30502909$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lessard, Martin</creatorcontrib><creatorcontrib>Blais, Mylène</creatorcontrib><creatorcontrib>Beaudoin, Frédéric</creatorcontrib><creatorcontrib>Deschene, Karine</creatorcontrib><creatorcontrib>Verso, Luca Lo</creatorcontrib><creatorcontrib>Bissonnette, Nathalie</creatorcontrib><creatorcontrib>Lauzon, Karoline</creatorcontrib><creatorcontrib>Guay, Frédéric</creatorcontrib><title>Piglet weight gain during the first two weeks of lactation influences the immune system development</title><title>Veterinary immunology and immunopathology</title><addtitle>Vet Immunol Immunopathol</addtitle><description>•Piglets’ immune system development is affected by birth weight and early growth performances.•Expression of genes involved in defense response and oxidative stress was modulated in LWG piglets.•Intestinal antigen presenting cells markers (APC) were decreased in LWG piglets.•The increase of B cells in HWG piglets was not observed in LWG piglets.•The regulation of various biological processes supporting neonatal development is associated to maternal lactocrine signaling.
The aim of this study was to investigate the effect of the piglet growth during the first week of life on ileal expression of genes and on development of the immune system. Eight litters adjusted to 12 piglets were used. Within each litter, the piglet that showed the lowest weight gain (LWG; n = 8) and the one that showed the highest weight gain (HWG; n = 8) in their first week of life were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated on days 8 and 16 to characterize cellular population profiles and to assess ex-vivo secretion of interleukin-10 (IL-10), IL-6 and tumor necrosis factor-α (TNF-α). On day 16, piglets were euthanized and ileum samples were collected to extract RNA for microarray analysis and gene expression by qPCR. As expected, growth performance of LWG piglet was impaired compared to HWG piglets (P < 0.05). From day 8 to 16, the percentage of CD21+ B cells significantly increased in blood of heavier HWG piglets while the percentage remained constant in smaller LWG piglets (P weight x day = 0.01). For the CD4+CD8α− Th cells, a marked increase was observed in LWG piglets from 8 to 16 days of age (P = 0.002) whereas no significant change occurred in HWG piglets. Percentages of CD14+ monocytes and other MHC-II+ cells were respectively higher and lower on day 8 compared to day 16 for both groups of piglets (P < 0.01). On day 8, LPS-activated PBMC from LWG piglets produced less IL-6 compared to HWG piglets (P < 0.05). Microarray analysis of gene expression in piglets’ ileum tissue indicated that several genes involed in defense response and response to oxidative stress were modulated differently in LWG compared to HWG. Gene analysis by Q-PCR confirmed microarray results and revealed that IL-10, SOD1, NOS2, NOD2, TLR4, TLR9, CD40 and CD74 expressions were significantly decreased (P < 0.05) in LWG in comparison to HWG piglets, while MYD88 and NFkBiA showed a tendency to decrease (0.05 ≤ P < 0.07). These results suggest that birth weight and milk intake affect the growth performances and the development of immunity by modulating the expression of genes associated with immunity and oxidative stress in piglets’ intestinal tissue, and by affecting the leukocyte populations involved in innate and cell-mediated immunity in nursing piglets. Therefore, impaired development of immune system in LWG piglets might have an impact on their resistance to infections later in life.</description><subject>Animals</subject><subject>Cytokines - blood</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Ileum - anatomy & histology</subject><subject>Ileum - growth & development</subject><subject>Ileum - immunology</subject><subject>Immune System - growth & development</subject><subject>Immune system development</subject><subject>Lactation</subject><subject>Leukocytes, Mononuclear - immunology</subject><subject>Microarray Analysis - veterinary</subject><subject>Piglet</subject><subject>Real-Time Polymerase Chain Reaction - veterinary</subject><subject>Swine - growth & development</subject><subject>Swine - immunology</subject><subject>Weight gain</subject><subject>Weight Gain - immunology</subject><issn>0165-2427</issn><issn>1873-2534</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtOwzAQRS0EouXxBwh5ySbBduIk3iAhxEuqBAtYW7E9CS55FNtpxd_jUmDJajbnzp05CJ1RklJCi8tluoZg-z5lhFYppSkhfA_NaVVmCeNZvo_mEeMJy1k5Q0feL0kkRFUdollGOGGCiDnSz7btIOAN2PYt4La2AzaTs0OLwxvgxjofcNiMEYB3j8cGd7UOdbDjgO3QdBMMGvw3G2-ZBsD-0wfosYE1dOOqhyGcoIOm7jyc_sxj9Hp3-3LzkCye7h9vrheJzgoWEqW5otTQPFecEWNEIaAoVa6N5jwzTBRFpQSj2hidVYqCynStFAApCK9NmR2ji93elRs_JvBB9tZr6Lp6gHHyktFcEEZESSKa71DtRu8dNHLlbF-7T0mJ3OqVS7nTK7d6JaUyyoux85-GSfVg_kK_PiNwtQMg_rm24KTXdqvIWAc6SDPa_xu-ALfgj9w</recordid><startdate>201812</startdate><enddate>201812</enddate><creator>Lessard, Martin</creator><creator>Blais, Mylène</creator><creator>Beaudoin, Frédéric</creator><creator>Deschene, Karine</creator><creator>Verso, Luca Lo</creator><creator>Bissonnette, Nathalie</creator><creator>Lauzon, Karoline</creator><creator>Guay, Frédéric</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0001-6964-1293</orcidid><orcidid>https://orcid.org/0000-0002-5771-566X</orcidid><orcidid>https://orcid.org/0000-0003-3281-209X</orcidid></search><sort><creationdate>201812</creationdate><title>Piglet weight gain during the first two weeks of lactation influences the immune system development</title><author>Lessard, Martin ; Blais, Mylène ; Beaudoin, Frédéric ; Deschene, Karine ; Verso, Luca Lo ; Bissonnette, Nathalie ; Lauzon, Karoline ; Guay, Frédéric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c362t-bc5b11d144b520dd969e67b4cdc553d29668b921cddc38b1eb3cabbee0605ad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Animals</topic><topic>Cytokines - blood</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Ileum - anatomy & histology</topic><topic>Ileum - growth & development</topic><topic>Ileum - immunology</topic><topic>Immune System - growth & development</topic><topic>Immune system development</topic><topic>Lactation</topic><topic>Leukocytes, Mononuclear - immunology</topic><topic>Microarray Analysis - veterinary</topic><topic>Piglet</topic><topic>Real-Time Polymerase Chain Reaction - veterinary</topic><topic>Swine - growth & development</topic><topic>Swine - immunology</topic><topic>Weight gain</topic><topic>Weight Gain - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lessard, Martin</creatorcontrib><creatorcontrib>Blais, Mylène</creatorcontrib><creatorcontrib>Beaudoin, Frédéric</creatorcontrib><creatorcontrib>Deschene, Karine</creatorcontrib><creatorcontrib>Verso, Luca Lo</creatorcontrib><creatorcontrib>Bissonnette, Nathalie</creatorcontrib><creatorcontrib>Lauzon, Karoline</creatorcontrib><creatorcontrib>Guay, Frédéric</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary immunology and immunopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lessard, Martin</au><au>Blais, Mylène</au><au>Beaudoin, Frédéric</au><au>Deschene, Karine</au><au>Verso, Luca Lo</au><au>Bissonnette, Nathalie</au><au>Lauzon, Karoline</au><au>Guay, Frédéric</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Piglet weight gain during the first two weeks of lactation influences the immune system development</atitle><jtitle>Veterinary immunology and immunopathology</jtitle><addtitle>Vet Immunol Immunopathol</addtitle><date>2018-12</date><risdate>2018</risdate><volume>206</volume><spage>25</spage><epage>34</epage><pages>25-34</pages><issn>0165-2427</issn><eissn>1873-2534</eissn><abstract>•Piglets’ immune system development is affected by birth weight and early growth performances.•Expression of genes involved in defense response and oxidative stress was modulated in LWG piglets.•Intestinal antigen presenting cells markers (APC) were decreased in LWG piglets.•The increase of B cells in HWG piglets was not observed in LWG piglets.•The regulation of various biological processes supporting neonatal development is associated to maternal lactocrine signaling.
The aim of this study was to investigate the effect of the piglet growth during the first week of life on ileal expression of genes and on development of the immune system. Eight litters adjusted to 12 piglets were used. Within each litter, the piglet that showed the lowest weight gain (LWG; n = 8) and the one that showed the highest weight gain (HWG; n = 8) in their first week of life were enrolled. Peripheral blood mononuclear cells (PBMC) were isolated on days 8 and 16 to characterize cellular population profiles and to assess ex-vivo secretion of interleukin-10 (IL-10), IL-6 and tumor necrosis factor-α (TNF-α). On day 16, piglets were euthanized and ileum samples were collected to extract RNA for microarray analysis and gene expression by qPCR. As expected, growth performance of LWG piglet was impaired compared to HWG piglets (P < 0.05). From day 8 to 16, the percentage of CD21+ B cells significantly increased in blood of heavier HWG piglets while the percentage remained constant in smaller LWG piglets (P weight x day = 0.01). For the CD4+CD8α− Th cells, a marked increase was observed in LWG piglets from 8 to 16 days of age (P = 0.002) whereas no significant change occurred in HWG piglets. Percentages of CD14+ monocytes and other MHC-II+ cells were respectively higher and lower on day 8 compared to day 16 for both groups of piglets (P < 0.01). On day 8, LPS-activated PBMC from LWG piglets produced less IL-6 compared to HWG piglets (P < 0.05). Microarray analysis of gene expression in piglets’ ileum tissue indicated that several genes involed in defense response and response to oxidative stress were modulated differently in LWG compared to HWG. Gene analysis by Q-PCR confirmed microarray results and revealed that IL-10, SOD1, NOS2, NOD2, TLR4, TLR9, CD40 and CD74 expressions were significantly decreased (P < 0.05) in LWG in comparison to HWG piglets, while MYD88 and NFkBiA showed a tendency to decrease (0.05 ≤ P < 0.07). These results suggest that birth weight and milk intake affect the growth performances and the development of immunity by modulating the expression of genes associated with immunity and oxidative stress in piglets’ intestinal tissue, and by affecting the leukocyte populations involved in innate and cell-mediated immunity in nursing piglets. Therefore, impaired development of immune system in LWG piglets might have an impact on their resistance to infections later in life.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>30502909</pmid><doi>10.1016/j.vetimm.2018.11.005</doi><tpages>10</tpages><orcidid>https://orcid.org/0000-0001-6964-1293</orcidid><orcidid>https://orcid.org/0000-0002-5771-566X</orcidid><orcidid>https://orcid.org/0000-0003-3281-209X</orcidid></addata></record> |
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| subjects | Animals Cytokines - blood Enzyme-Linked Immunosorbent Assay - veterinary Female Gene Expression Ileum - anatomy & histology Ileum - growth & development Ileum - immunology Immune System - growth & development Immune system development Lactation Leukocytes, Mononuclear - immunology Microarray Analysis - veterinary Piglet Real-Time Polymerase Chain Reaction - veterinary Swine - growth & development Swine - immunology Weight gain Weight Gain - immunology |
| title | Piglet weight gain during the first two weeks of lactation influences the immune system development |
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