Poly-DL-lactide-poly(ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen

This project was aimed at illustrating the potential use of poly‐DL‐lactide–poly(ethylene glycol) (PELA) microspheres as a hepatitis B surface antigen (HBsAg) delivery system following subcutaneous (s.c.) or oral immunization over the current injection of an alum‐absorbed antigen. The antigen‐loadin...

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Veröffentlicht in:Journal of applied polymer science 2002-01, Vol.83 (4), p.850-856
Hauptverfasser: Li, Xiaohong, Zhou, Shaobing, Wu, Xiaorong, Yuan, Minglong, Liu, Li, Jia, Wenxiang, Deng, Xianmo, Huang, Zhitang
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container_end_page 856
container_issue 4
container_start_page 850
container_title Journal of applied polymer science
container_volume 83
creator Li, Xiaohong
Zhou, Shaobing
Wu, Xiaorong
Yuan, Minglong
Liu, Li
Jia, Wenxiang
Deng, Xianmo
Huang, Zhitang
description This project was aimed at illustrating the potential use of poly‐DL‐lactide–poly(ethylene glycol) (PELA) microspheres as a hepatitis B surface antigen (HBsAg) delivery system following subcutaneous (s.c.) or oral immunization over the current injection of an alum‐absorbed antigen. The antigen‐loading microspheres were elaborated by the solvent‐extraction method based on the formation of modified multiple w/o/w emulsion. The microspheres were characterized by their particle size, HBsAg entrapment, and in vitro HBsAg release behavior. Balb/c mice were immunized with an s.c. injection and oral administration of a single dose and two doses of a microsphere formulation. For comparison, the alum‐absorbed HBsAg‐immunized mice had a following intramuscular (i.m.) injection at weeks 0 and 4. At weeks 8, 10, 14, and 24 postadministration, the blood and saliva samples were collected and detected by the enzyme‐linked immunosorbed assay (ELISA) method. A single injection of HBsAg/PELA microspheres could induce a serum lgG antibody level comparable to the two‐injection alum‐absorbed HBsAg at the 14th week and higher than that at the 24th week. The saliva lgA of peroral groups was significantly higher than that of the s.c. injection of a microsphere formulation and i.m. injection of soluble antigen. These preliminary results demonstrated the potential of oral administration of antigen‐loading microspheres in the induction of a secretory immune response, and it is suggested that a single‐dose s.c. injection of antigen‐loading microspheres would be an efficient immunization schedule to elicit a protective response. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 850–856, 2002
doi_str_mv 10.1002/app.10081
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The antigen‐loading microspheres were elaborated by the solvent‐extraction method based on the formation of modified multiple w/o/w emulsion. The microspheres were characterized by their particle size, HBsAg entrapment, and in vitro HBsAg release behavior. Balb/c mice were immunized with an s.c. injection and oral administration of a single dose and two doses of a microsphere formulation. For comparison, the alum‐absorbed HBsAg‐immunized mice had a following intramuscular (i.m.) injection at weeks 0 and 4. At weeks 8, 10, 14, and 24 postadministration, the blood and saliva samples were collected and detected by the enzyme‐linked immunosorbed assay (ELISA) method. A single injection of HBsAg/PELA microspheres could induce a serum lgG antibody level comparable to the two‐injection alum‐absorbed HBsAg at the 14th week and higher than that at the 24th week. The saliva lgA of peroral groups was significantly higher than that of the s.c. injection of a microsphere formulation and i.m. injection of soluble antigen. These preliminary results demonstrated the potential of oral administration of antigen‐loading microspheres in the induction of a secretory immune response, and it is suggested that a single‐dose s.c. injection of antigen‐loading microspheres would be an efficient immunization schedule to elicit a protective response. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 850–856, 2002</description><identifier>ISSN: 0021-8995</identifier><identifier>EISSN: 1097-4628</identifier><identifier>DOI: 10.1002/app.10081</identifier><identifier>CODEN: JAPNAB</identifier><language>eng</language><publisher>New York: Wiley Periodicals, Inc</publisher><subject>Antibodies ; Antigens ; Applied sciences ; biodegradable ; Biodegradation ; Controlled drug delivery ; drug delivery systems ; Emulsions ; Enzymes ; Exact sciences and technology ; Immunization ; microencapsulation ; Organic polymers ; Particle size analysis ; Physicochemistry of polymers ; polyester ; Properties and characterization ; protein ; Proteins ; Solution and gel properties ; Solvent extraction</subject><ispartof>Journal of applied polymer science, 2002-01, Vol.83 (4), p.850-856</ispartof><rights>Copyright © 2002 Wiley Periodicals, Inc.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4071-284ec7f7a07078b295095204652386477dd44fd87ad77cb256787d230141a1963</citedby><cites>FETCH-LOGICAL-c4071-284ec7f7a07078b295095204652386477dd44fd87ad77cb256787d230141a1963</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fapp.10081$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fapp.10081$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=14121308$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Li, Xiaohong</creatorcontrib><creatorcontrib>Zhou, Shaobing</creatorcontrib><creatorcontrib>Wu, Xiaorong</creatorcontrib><creatorcontrib>Yuan, Minglong</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Jia, Wenxiang</creatorcontrib><creatorcontrib>Deng, Xianmo</creatorcontrib><creatorcontrib>Huang, Zhitang</creatorcontrib><title>Poly-DL-lactide-poly(ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen</title><title>Journal of applied polymer science</title><addtitle>J. Appl. Polym. Sci</addtitle><description>This project was aimed at illustrating the potential use of poly‐DL‐lactide–poly(ethylene glycol) (PELA) microspheres as a hepatitis B surface antigen (HBsAg) delivery system following subcutaneous (s.c.) or oral immunization over the current injection of an alum‐absorbed antigen. The antigen‐loading microspheres were elaborated by the solvent‐extraction method based on the formation of modified multiple w/o/w emulsion. The microspheres were characterized by their particle size, HBsAg entrapment, and in vitro HBsAg release behavior. Balb/c mice were immunized with an s.c. injection and oral administration of a single dose and two doses of a microsphere formulation. For comparison, the alum‐absorbed HBsAg‐immunized mice had a following intramuscular (i.m.) injection at weeks 0 and 4. At weeks 8, 10, 14, and 24 postadministration, the blood and saliva samples were collected and detected by the enzyme‐linked immunosorbed assay (ELISA) method. A single injection of HBsAg/PELA microspheres could induce a serum lgG antibody level comparable to the two‐injection alum‐absorbed HBsAg at the 14th week and higher than that at the 24th week. The saliva lgA of peroral groups was significantly higher than that of the s.c. injection of a microsphere formulation and i.m. injection of soluble antigen. These preliminary results demonstrated the potential of oral administration of antigen‐loading microspheres in the induction of a secretory immune response, and it is suggested that a single‐dose s.c. injection of antigen‐loading microspheres would be an efficient immunization schedule to elicit a protective response. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 850–856, 2002</description><subject>Antibodies</subject><subject>Antigens</subject><subject>Applied sciences</subject><subject>biodegradable</subject><subject>Biodegradation</subject><subject>Controlled drug delivery</subject><subject>drug delivery systems</subject><subject>Emulsions</subject><subject>Enzymes</subject><subject>Exact sciences and technology</subject><subject>Immunization</subject><subject>microencapsulation</subject><subject>Organic polymers</subject><subject>Particle size analysis</subject><subject>Physicochemistry of polymers</subject><subject>polyester</subject><subject>Properties and characterization</subject><subject>protein</subject><subject>Proteins</subject><subject>Solution and gel properties</subject><subject>Solvent extraction</subject><issn>0021-8995</issn><issn>1097-4628</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNp1kEFv1DAQha2KSl1aDv0HvoDowdR2nNg5lkJbxKpdIapKvVhTZ9I1eJNgZ6Hh1-NlSzn1NDOa773RPEIOBX8nOJfHMAybxogdMhO81kxV0rwgs7wTzNR1uUdepvSNcyFKXs3I70UfJvZhzgK40TfIhjy_xXE5BeyQ3ofJ9eGIrryLfRqWGDFRSLSPECh0DR0gYjfiZmww-J8YJ5qmNOIq0baPdIkDjH70ib6naR1bcJh1o7_H7oDsthASvnqs--T67OPX0ws2vzr_dHoyZ05xLZg0Cp1uNXDNtbmTdcnrUnJVlbIwldK6aZRqG6Oh0drdybLSRjey4EIJEHVV7JM3W98h9j_WmEa78slhCNBhv05WCmVUPpXBoy24-TVFbO0Q_QriZAW3m3RtTtf-TTezrx9NITkIbYTO-fRfoIQUBTeZO95yv3zA6XlDe7JY_HNmW4XPKT48KSB-t5UudGlvLs-tuPh8Wymh7JfiD1rZl7A</recordid><startdate>20020124</startdate><enddate>20020124</enddate><creator>Li, Xiaohong</creator><creator>Zhou, Shaobing</creator><creator>Wu, Xiaorong</creator><creator>Yuan, Minglong</creator><creator>Liu, Li</creator><creator>Jia, Wenxiang</creator><creator>Deng, Xianmo</creator><creator>Huang, Zhitang</creator><general>Wiley Periodicals, Inc</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20020124</creationdate><title>Poly-DL-lactide-poly(ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen</title><author>Li, Xiaohong ; Zhou, Shaobing ; Wu, Xiaorong ; Yuan, Minglong ; Liu, Li ; Jia, Wenxiang ; Deng, Xianmo ; Huang, Zhitang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4071-284ec7f7a07078b295095204652386477dd44fd87ad77cb256787d230141a1963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Antibodies</topic><topic>Antigens</topic><topic>Applied sciences</topic><topic>biodegradable</topic><topic>Biodegradation</topic><topic>Controlled drug delivery</topic><topic>drug delivery systems</topic><topic>Emulsions</topic><topic>Enzymes</topic><topic>Exact sciences and technology</topic><topic>Immunization</topic><topic>microencapsulation</topic><topic>Organic polymers</topic><topic>Particle size analysis</topic><topic>Physicochemistry of polymers</topic><topic>polyester</topic><topic>Properties and characterization</topic><topic>protein</topic><topic>Proteins</topic><topic>Solution and gel properties</topic><topic>Solvent extraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Li, Xiaohong</creatorcontrib><creatorcontrib>Zhou, Shaobing</creatorcontrib><creatorcontrib>Wu, Xiaorong</creatorcontrib><creatorcontrib>Yuan, Minglong</creatorcontrib><creatorcontrib>Liu, Li</creatorcontrib><creatorcontrib>Jia, Wenxiang</creatorcontrib><creatorcontrib>Deng, Xianmo</creatorcontrib><creatorcontrib>Huang, Zhitang</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of applied polymer science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Li, Xiaohong</au><au>Zhou, Shaobing</au><au>Wu, Xiaorong</au><au>Yuan, Minglong</au><au>Liu, Li</au><au>Jia, Wenxiang</au><au>Deng, Xianmo</au><au>Huang, Zhitang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Poly-DL-lactide-poly(ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen</atitle><jtitle>Journal of applied polymer science</jtitle><addtitle>J. Appl. Polym. Sci</addtitle><date>2002-01-24</date><risdate>2002</risdate><volume>83</volume><issue>4</issue><spage>850</spage><epage>856</epage><pages>850-856</pages><issn>0021-8995</issn><eissn>1097-4628</eissn><coden>JAPNAB</coden><abstract>This project was aimed at illustrating the potential use of poly‐DL‐lactide–poly(ethylene glycol) (PELA) microspheres as a hepatitis B surface antigen (HBsAg) delivery system following subcutaneous (s.c.) or oral immunization over the current injection of an alum‐absorbed antigen. The antigen‐loading microspheres were elaborated by the solvent‐extraction method based on the formation of modified multiple w/o/w emulsion. The microspheres were characterized by their particle size, HBsAg entrapment, and in vitro HBsAg release behavior. Balb/c mice were immunized with an s.c. injection and oral administration of a single dose and two doses of a microsphere formulation. For comparison, the alum‐absorbed HBsAg‐immunized mice had a following intramuscular (i.m.) injection at weeks 0 and 4. At weeks 8, 10, 14, and 24 postadministration, the blood and saliva samples were collected and detected by the enzyme‐linked immunosorbed assay (ELISA) method. A single injection of HBsAg/PELA microspheres could induce a serum lgG antibody level comparable to the two‐injection alum‐absorbed HBsAg at the 14th week and higher than that at the 24th week. The saliva lgA of peroral groups was significantly higher than that of the s.c. injection of a microsphere formulation and i.m. injection of soluble antigen. These preliminary results demonstrated the potential of oral administration of antigen‐loading microspheres in the induction of a secretory immune response, and it is suggested that a single‐dose s.c. injection of antigen‐loading microspheres would be an efficient immunization schedule to elicit a protective response. © 2002 Wiley Periodicals, Inc. J Appl Polym Sci 83: 850–856, 2002</abstract><cop>New York</cop><pub>Wiley Periodicals, Inc</pub><doi>10.1002/app.10081</doi><tpages>7</tpages></addata></record>
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subjects Antibodies
Antigens
Applied sciences
biodegradable
Biodegradation
Controlled drug delivery
drug delivery systems
Emulsions
Enzymes
Exact sciences and technology
Immunization
microencapsulation
Organic polymers
Particle size analysis
Physicochemistry of polymers
polyester
Properties and characterization
protein
Proteins
Solution and gel properties
Solvent extraction
title Poly-DL-lactide-poly(ethylene glycol) microspheres as oral and parenteral delivery systems for hepatitis B surface antigen
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