RP-hPLC/ESI MS determination of acyl chain positions in phospholipids
Since phospholipids are used as excipients in many pharmaceutical products, there is a need for validated strategies to characterize the phospholipid constituents. We describe a reversed phase HPLC/electrospray ionization mass spectrometry method that is an alternative to the laborious phospholipase...
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| Veröffentlicht in: | Journal of separation science 2002-04, Vol.25 (5-6), p.285-289 |
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| container_end_page | 289 |
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| container_issue | 5-6 |
| container_start_page | 285 |
| container_title | Journal of separation science |
| container_volume | 25 |
| creator | Vernooij, Elisabeth A. A. M. Brouwers, Jos F. H. M. Kettenes-Van den Bosch, J. Jantina Crommelin, Daan J. A. |
| description | Since phospholipids are used as excipients in many pharmaceutical products, there is a need for validated strategies to characterize the phospholipid constituents. We describe a reversed phase HPLC/electrospray ionization mass spectrometry method that is an alternative to the laborious phospholipase A2 treatment commonly used to determine the acyl chain positions in phospholipids. This reversed phase HPLC/electrospray mass spectrometry system (a) shows good chromatographic resolution for phosphatidylcholine molecular species, (b) allows determination of the composition of complex mixtures of phosphatidylcholines, and, most important, (c) allows unequivocal assignment of the positions of the acyl chains on the glycerol backbone in mixtures of POPC and OPPC and in mixtures of POPE and OPPE. |
| doi_str_mv | 10.1002/1615-9314(20020401)25:5/6<285::AID-JSSC285>3.0.CO;2-U |
| format | Article |
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This reversed phase HPLC/electrospray mass spectrometry system (a) shows good chromatographic resolution for phosphatidylcholine molecular species, (b) allows determination of the composition of complex mixtures of phosphatidylcholines, and, most important, (c) allows unequivocal assignment of the positions of the acyl chains on the glycerol backbone in mixtures of POPC and OPPC and in mixtures of POPE and OPPE.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/1615-9314(20020401)25:5/6<285::AID-JSSC285>3.0.CO;2-U</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Analysis ; Analytical chemistry ; Biological and medical sciences ; Chemistry ; Chromatographic analysis ; Chromatographic methods and physical methods associated with chromatography ; Drug products ; Electrospray ionization ; Exact sciences and technology ; General pharmacology ; Glycerol ; HPLC ; Ionization ; Mass spectrometry ; Medical sciences ; Other chromatographic methods ; Pharmacology. 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This reversed phase HPLC/electrospray mass spectrometry system (a) shows good chromatographic resolution for phosphatidylcholine molecular species, (b) allows determination of the composition of complex mixtures of phosphatidylcholines, and, most important, (c) allows unequivocal assignment of the positions of the acyl chains on the glycerol backbone in mixtures of POPC and OPPC and in mixtures of POPE and OPPE.</description><subject>Analysis</subject><subject>Analytical chemistry</subject><subject>Biological and medical sciences</subject><subject>Chemistry</subject><subject>Chromatographic analysis</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Drug products</subject><subject>Electrospray ionization</subject><subject>Exact sciences and technology</subject><subject>General pharmacology</subject><subject>Glycerol</subject><subject>HPLC</subject><subject>Ionization</subject><subject>Mass spectrometry</subject><subject>Medical sciences</subject><subject>Other chromatographic methods</subject><subject>Pharmacology. 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Drug treatments</topic><topic>Phospholipids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vernooij, Elisabeth A. A. M.</creatorcontrib><creatorcontrib>Brouwers, Jos F. H. M.</creatorcontrib><creatorcontrib>Kettenes-Van den Bosch, J. Jantina</creatorcontrib><creatorcontrib>Crommelin, Daan J. A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vernooij, Elisabeth A. A. M.</au><au>Brouwers, Jos F. H. M.</au><au>Kettenes-Van den Bosch, J. Jantina</au><au>Crommelin, Daan J. A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RP-hPLC/ESI MS determination of acyl chain positions in phospholipids</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. 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| fulltext | fulltext |
| identifier | ISSN: 1615-9306 |
| ispartof | Journal of separation science, 2002-04, Vol.25 (5-6), p.285-289 |
| issn | 1615-9306 1615-9314 |
| language | eng |
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| source | Wiley Online Library Journals Frontfile Complete |
| subjects | Analysis Analytical chemistry Biological and medical sciences Chemistry Chromatographic analysis Chromatographic methods and physical methods associated with chromatography Drug products Electrospray ionization Exact sciences and technology General pharmacology Glycerol HPLC Ionization Mass spectrometry Medical sciences Other chromatographic methods Pharmacology. Drug treatments Phospholipids |
| title | RP-hPLC/ESI MS determination of acyl chain positions in phospholipids |
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