LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants
Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number of genes, including many virulence factors. Most studies of QS in P. aeruginosa have been performed in clinical isolates and have therefore focused on its role in pathogenicity. P. ae...
Gespeichert in:
| Veröffentlicht in: | Applied and Environmental Microbiology 2009-08, Vol.75 (15), p.5131-5140 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5140 |
|---|---|
| container_issue | 15 |
| container_start_page | 5131 |
| container_title | Applied and Environmental Microbiology |
| container_volume | 75 |
| creator | Steindler, Laura Bertani, Iris De Sordi, Luisa Schwager, Stephan Eberl, Leo Venturi, Vittorio |
| description | Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number of genes, including many virulence factors. Most studies of QS in P. aeruginosa have been performed in clinical isolates and have therefore focused on its role in pathogenicity. P. aeruginosa, however, is regarded as a ubiquitous organism capable of colonizing many different environments and also of establishing beneficial associations with plants. In this study we examined the role of the two N-acyl homoserine lactone systems known as RhlI/R and LasI/R in the environmental rice rhizosphere isolate P. aeruginosa PUPa3. Both the Rhl and Las systems are involved in the regulation of plant growth-promoting traits. The environmental P. aeruginosa PUPa3 is pathogenic in two nonmammalian infection models, and only the double las rhl mutants are attenuated for virulence. In fact it was established that the two QS systems are not hierarchically organized and that they are both important for the colonization of the rice rhizosphere. This is an in-depth genetic and molecular study of QS in an environmental P. aeruginosa strain and highlights several differences with QS regulation in the clinical isolate PAO1. |
| doi_str_mv | 10.1128/AEM.02914-08 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_21479072</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67523865</sourcerecordid><originalsourceid>FETCH-LOGICAL-c456t-ae915b66577b69f439f69dc825a999cde6f4d7bdb319e491ad231f637307858a3</originalsourceid><addsrcrecordid>eNqF0c1rFDEYBvBBFLut3jxrEPTktPn-uBRqqVpYsXbtyUN4ZyazG5lJajJT8b836y5VT57yQn48yctTVc8IPiaE6pOzi4_HmBrCa6wfVAuCja4FY_JhtcDYmJpSjg-qw5y_YYw5lvpxdUCMoIIqsai-LiFfnlwjCB263gzb8fMc0zyilQvZhzXyAQFaTQnKEHt0ld3cxTEGyAhcmtc-xAzorQuu962HAU0RXQ0QpvyketTDkN3T_XlU3by7-HL-oV5-en95frasWy7kVIMzRDRSCqUaaXrOTC9N12oqwBjTdk72vFNN1zBiHDcEOspIL5liWGmhgR1Vp7vc27kZXde6UH472NvkR0g_bQRv_70JfmPX8c5SRQXXvAS83gek-H12ebKjz60byhYuztlKJSjTUvwXUsKVwYoW-GYH2xRzTq6__w3BdlubLbXZ37VZrAt__vcGf_C-pwJe7QHkFoY-QWh9vneUKKMJJcW93LmNX29--OQs5NGCG60SlggrCNuiFzvUQ7SwTiXoZkUxYZhIoXl57hfIRLOc</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21479072</pqid></control><display><type>article</type><title>LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants</title><source>MEDLINE</source><source>American Society for Microbiology Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>EZB Electronic Journals Library</source><creator>Steindler, Laura ; Bertani, Iris ; De Sordi, Luisa ; Schwager, Stephan ; Eberl, Leo ; Venturi, Vittorio</creator><creatorcontrib>Steindler, Laura ; Bertani, Iris ; De Sordi, Luisa ; Schwager, Stephan ; Eberl, Leo ; Venturi, Vittorio</creatorcontrib><description>Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number of genes, including many virulence factors. Most studies of QS in P. aeruginosa have been performed in clinical isolates and have therefore focused on its role in pathogenicity. P. aeruginosa, however, is regarded as a ubiquitous organism capable of colonizing many different environments and also of establishing beneficial associations with plants. In this study we examined the role of the two N-acyl homoserine lactone systems known as RhlI/R and LasI/R in the environmental rice rhizosphere isolate P. aeruginosa PUPa3. Both the Rhl and Las systems are involved in the regulation of plant growth-promoting traits. The environmental P. aeruginosa PUPa3 is pathogenic in two nonmammalian infection models, and only the double las rhl mutants are attenuated for virulence. In fact it was established that the two QS systems are not hierarchically organized and that they are both important for the colonization of the rice rhizosphere. This is an in-depth genetic and molecular study of QS in an environmental P. aeruginosa strain and highlights several differences with QS regulation in the clinical isolate PAO1.</description><identifier>ISSN: 0099-2240</identifier><identifier>EISSN: 1098-5336</identifier><identifier>EISSN: 1098-6596</identifier><identifier>DOI: 10.1128/AEM.02914-08</identifier><identifier>PMID: 19525275</identifier><identifier>CODEN: AEMIDF</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - physiology ; Biological and medical sciences ; Caenorhabditis elegans - microbiology ; Clinical isolates ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Genetics and Molecular Biology ; Ligases - genetics ; Ligases - physiology ; Microbiology ; Molecular Sequence Data ; Moths - microbiology ; Oryza - microbiology ; Oryza sativa ; Plant Roots - microbiology ; Pseudomonas aeruginosa - genetics ; Pseudomonas aeruginosa - pathogenicity ; Pseudomonas aeruginosa - physiology ; Pseudomonas Infections - microbiology ; Quorum Sensing ; Sequence Analysis, DNA ; Survival Analysis ; Trans-Activators - genetics ; Trans-Activators - physiology ; Transcription Factors - genetics ; Transcription Factors - physiology ; Virulence</subject><ispartof>Applied and Environmental Microbiology, 2009-08, Vol.75 (15), p.5131-5140</ispartof><rights>2009 INIST-CNRS</rights><rights>Copyright © 2009, American Society for Microbiology</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c456t-ae915b66577b69f439f69dc825a999cde6f4d7bdb319e491ad231f637307858a3</citedby><cites>FETCH-LOGICAL-c456t-ae915b66577b69f439f69dc825a999cde6f4d7bdb319e491ad231f637307858a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725484/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725484/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,723,776,780,881,3175,3176,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21798121$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19525275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Steindler, Laura</creatorcontrib><creatorcontrib>Bertani, Iris</creatorcontrib><creatorcontrib>De Sordi, Luisa</creatorcontrib><creatorcontrib>Schwager, Stephan</creatorcontrib><creatorcontrib>Eberl, Leo</creatorcontrib><creatorcontrib>Venturi, Vittorio</creatorcontrib><title>LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants</title><title>Applied and Environmental Microbiology</title><addtitle>Appl Environ Microbiol</addtitle><description>Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number of genes, including many virulence factors. Most studies of QS in P. aeruginosa have been performed in clinical isolates and have therefore focused on its role in pathogenicity. P. aeruginosa, however, is regarded as a ubiquitous organism capable of colonizing many different environments and also of establishing beneficial associations with plants. In this study we examined the role of the two N-acyl homoserine lactone systems known as RhlI/R and LasI/R in the environmental rice rhizosphere isolate P. aeruginosa PUPa3. Both the Rhl and Las systems are involved in the regulation of plant growth-promoting traits. The environmental P. aeruginosa PUPa3 is pathogenic in two nonmammalian infection models, and only the double las rhl mutants are attenuated for virulence. In fact it was established that the two QS systems are not hierarchically organized and that they are both important for the colonization of the rice rhizosphere. This is an in-depth genetic and molecular study of QS in an environmental P. aeruginosa strain and highlights several differences with QS regulation in the clinical isolate PAO1.</description><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - physiology</subject><subject>Biological and medical sciences</subject><subject>Caenorhabditis elegans - microbiology</subject><subject>Clinical isolates</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetics and Molecular Biology</subject><subject>Ligases - genetics</subject><subject>Ligases - physiology</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Moths - microbiology</subject><subject>Oryza - microbiology</subject><subject>Oryza sativa</subject><subject>Plant Roots - microbiology</subject><subject>Pseudomonas aeruginosa - genetics</subject><subject>Pseudomonas aeruginosa - pathogenicity</subject><subject>Pseudomonas aeruginosa - physiology</subject><subject>Pseudomonas Infections - microbiology</subject><subject>Quorum Sensing</subject><subject>Sequence Analysis, DNA</subject><subject>Survival Analysis</subject><subject>Trans-Activators - genetics</subject><subject>Trans-Activators - physiology</subject><subject>Transcription Factors - genetics</subject><subject>Transcription Factors - physiology</subject><subject>Virulence</subject><issn>0099-2240</issn><issn>1098-5336</issn><issn>1098-6596</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c1rFDEYBvBBFLut3jxrEPTktPn-uBRqqVpYsXbtyUN4ZyazG5lJajJT8b836y5VT57yQn48yctTVc8IPiaE6pOzi4_HmBrCa6wfVAuCja4FY_JhtcDYmJpSjg-qw5y_YYw5lvpxdUCMoIIqsai-LiFfnlwjCB263gzb8fMc0zyilQvZhzXyAQFaTQnKEHt0ld3cxTEGyAhcmtc-xAzorQuu962HAU0RXQ0QpvyketTDkN3T_XlU3by7-HL-oV5-en95frasWy7kVIMzRDRSCqUaaXrOTC9N12oqwBjTdk72vFNN1zBiHDcEOspIL5liWGmhgR1Vp7vc27kZXde6UH472NvkR0g_bQRv_70JfmPX8c5SRQXXvAS83gek-H12ebKjz60byhYuztlKJSjTUvwXUsKVwYoW-GYH2xRzTq6__w3BdlubLbXZ37VZrAt__vcGf_C-pwJe7QHkFoY-QWh9vneUKKMJJcW93LmNX29--OQs5NGCG60SlggrCNuiFzvUQ7SwTiXoZkUxYZhIoXl57hfIRLOc</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Steindler, Laura</creator><creator>Bertani, Iris</creator><creator>De Sordi, Luisa</creator><creator>Schwager, Stephan</creator><creator>Eberl, Leo</creator><creator>Venturi, Vittorio</creator><general>American Society for Microbiology</general><general>American Society for Microbiology (ASM)</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090801</creationdate><title>LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants</title><author>Steindler, Laura ; Bertani, Iris ; De Sordi, Luisa ; Schwager, Stephan ; Eberl, Leo ; Venturi, Vittorio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c456t-ae915b66577b69f439f69dc825a999cde6f4d7bdb319e491ad231f637307858a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - physiology</topic><topic>Biological and medical sciences</topic><topic>Caenorhabditis elegans - microbiology</topic><topic>Clinical isolates</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetics and Molecular Biology</topic><topic>Ligases - genetics</topic><topic>Ligases - physiology</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Moths - microbiology</topic><topic>Oryza - microbiology</topic><topic>Oryza sativa</topic><topic>Plant Roots - microbiology</topic><topic>Pseudomonas aeruginosa - genetics</topic><topic>Pseudomonas aeruginosa - pathogenicity</topic><topic>Pseudomonas aeruginosa - physiology</topic><topic>Pseudomonas Infections - microbiology</topic><topic>Quorum Sensing</topic><topic>Sequence Analysis, DNA</topic><topic>Survival Analysis</topic><topic>Trans-Activators - genetics</topic><topic>Trans-Activators - physiology</topic><topic>Transcription Factors - genetics</topic><topic>Transcription Factors - physiology</topic><topic>Virulence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steindler, Laura</creatorcontrib><creatorcontrib>Bertani, Iris</creatorcontrib><creatorcontrib>De Sordi, Luisa</creatorcontrib><creatorcontrib>Schwager, Stephan</creatorcontrib><creatorcontrib>Eberl, Leo</creatorcontrib><creatorcontrib>Venturi, Vittorio</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Applied and Environmental Microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steindler, Laura</au><au>Bertani, Iris</au><au>De Sordi, Luisa</au><au>Schwager, Stephan</au><au>Eberl, Leo</au><au>Venturi, Vittorio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants</atitle><jtitle>Applied and Environmental Microbiology</jtitle><addtitle>Appl Environ Microbiol</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>75</volume><issue>15</issue><spage>5131</spage><epage>5140</epage><pages>5131-5140</pages><issn>0099-2240</issn><eissn>1098-5336</eissn><eissn>1098-6596</eissn><coden>AEMIDF</coden><abstract>Pseudomonas aeruginosa possesses three quorum-sensing (QS) systems which are key in the expression of a large number of genes, including many virulence factors. Most studies of QS in P. aeruginosa have been performed in clinical isolates and have therefore focused on its role in pathogenicity. P. aeruginosa, however, is regarded as a ubiquitous organism capable of colonizing many different environments and also of establishing beneficial associations with plants. In this study we examined the role of the two N-acyl homoserine lactone systems known as RhlI/R and LasI/R in the environmental rice rhizosphere isolate P. aeruginosa PUPa3. Both the Rhl and Las systems are involved in the regulation of plant growth-promoting traits. The environmental P. aeruginosa PUPa3 is pathogenic in two nonmammalian infection models, and only the double las rhl mutants are attenuated for virulence. In fact it was established that the two QS systems are not hierarchically organized and that they are both important for the colonization of the rice rhizosphere. This is an in-depth genetic and molecular study of QS in an environmental P. aeruginosa strain and highlights several differences with QS regulation in the clinical isolate PAO1.</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>19525275</pmid><doi>10.1128/AEM.02914-08</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0099-2240 |
| ispartof | Applied and Environmental Microbiology, 2009-08, Vol.75 (15), p.5131-5140 |
| issn | 0099-2240 1098-5336 1098-6596 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21479072 |
| source | MEDLINE; American Society for Microbiology Journals; PubMed Central; Alma/SFX Local Collection; EZB Electronic Journals Library |
| subjects | Animals Bacterial Proteins - genetics Bacterial Proteins - physiology Biological and medical sciences Caenorhabditis elegans - microbiology Clinical isolates DNA, Bacterial - chemistry DNA, Bacterial - genetics Fundamental and applied biological sciences. Psychology Genetics and Molecular Biology Ligases - genetics Ligases - physiology Microbiology Molecular Sequence Data Moths - microbiology Oryza - microbiology Oryza sativa Plant Roots - microbiology Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - pathogenicity Pseudomonas aeruginosa - physiology Pseudomonas Infections - microbiology Quorum Sensing Sequence Analysis, DNA Survival Analysis Trans-Activators - genetics Trans-Activators - physiology Transcription Factors - genetics Transcription Factors - physiology Virulence |
| title | LasI/R and RhlI/R Quorum Sensing in a Strain of Pseudomonas aeruginosa Beneficial to Plants |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-29T12%3A51%3A36IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=LasI/R%20and%20RhlI/R%20Quorum%20Sensing%20in%20a%20Strain%20of%20Pseudomonas%20aeruginosa%20Beneficial%20to%20Plants&rft.jtitle=Applied%20and%20Environmental%20Microbiology&rft.au=Steindler,%20Laura&rft.date=2009-08-01&rft.volume=75&rft.issue=15&rft.spage=5131&rft.epage=5140&rft.pages=5131-5140&rft.issn=0099-2240&rft.eissn=1098-5336&rft.coden=AEMIDF&rft_id=info:doi/10.1128/AEM.02914-08&rft_dat=%3Cproquest_pasca%3E67523865%3C/proquest_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21479072&rft_id=info:pmid/19525275&rfr_iscdi=true |