A preliminary study of space- and ground-grown insulin crystals by X-ray diffraction and by light scattering tomography
For the first time, both X-ray diffraction and light scattering tomography were used to study the same set of space- and ground-grown protein crystals. X-ray diffraction of the insulin crystals grown from T6 insulin solutions by temperature reduction showed that space-grown crystals diffract to high...
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| Veröffentlicht in: | Journal of crystal growth 2001-11, Vol.232 (1), p.511-519 |
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| container_title | Journal of crystal growth |
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| creator | De Mattei, R.C Feigelson, R.S Bray, T.L DeLucas, L.J Symersky, J |
| description | For the first time, both X-ray diffraction and light scattering tomography were used to study the same set of space- and ground-grown protein crystals. X-ray diffraction of the insulin crystals grown from T6 insulin solutions by temperature reduction showed that space-grown crystals diffract to higher resolution then those grown on the ground. The light scattering tomographs revealed that the space-grown crystals contained very few spherical micro-defects while the ground-grown ones had large numbers of extended micro-defects and dislocations. The tomographs also gave insight into the methods of defect formation. This study marks the beginning of qualification of light scattering tomograph as a tool for determining the suitability of a protein for use in X-ray diffraction studies. |
| doi_str_mv | 10.1016/S0022-0248(01)01159-9 |
| format | Article |
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X-ray diffraction of the insulin crystals grown from T6 insulin solutions by temperature reduction showed that space-grown crystals diffract to higher resolution then those grown on the ground. The light scattering tomographs revealed that the space-grown crystals contained very few spherical micro-defects while the ground-grown ones had large numbers of extended micro-defects and dislocations. The tomographs also gave insight into the methods of defect formation. This study marks the beginning of qualification of light scattering tomograph as a tool for determining the suitability of a protein for use in X-ray diffraction studies.</description><subject>Aminoacids, peptides. Hormones. Neuropeptides</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Crystal defects</subject><subject>Crystal growth</subject><subject>Deformation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Light scattering</subject><subject>Molecular crystals</subject><subject>Proteins</subject><subject>Tomography</subject><subject>X ray diffraction analysis</subject><issn>0022-0248</issn><issn>1873-5002</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r3DAQhkVpoNu0P6GgQwnpQenow7V9KiE0HxDoIQn0JhRpvFHxSo4kJ_jfR7sbmmNPc3jfR6N5CPnC4YQD__H9BkAIBkJ1x8C_AedNz_p3ZMW7VrKmhu_J6l_lA_mY81-ACnJYkedTOiUc_cYHkxaay-wWGgeaJ2ORURMcXac4B8fqeA7UhzyPPlCbllzMmOn9Qv-wZBbq_DAkY4uPYYfVYPTrh0KzNaVg8mFNS9zEdTLTw_KJHAwVx8-v85Dcnf-6Pbtk178vrs5Or5lVUhZmxKCsE53s5D22TiJIpbZ_V8IictVLYYC7GjVigNaAE6I2RCNMB6pyh-Ro_-6U4uOMueiNzxbH0QSMc9aCq5ZDvy02-6JNMeeEg56S31QnmoPeatY7zXrrUAPXO826r9zX1wWmHjpWBcH6_AZzydsWau3nvob12CePSWfrMVh0PqEt2kX_n0Uv0e6RvA</recordid><startdate>20011101</startdate><enddate>20011101</enddate><creator>De Mattei, R.C</creator><creator>Feigelson, R.S</creator><creator>Bray, T.L</creator><creator>DeLucas, L.J</creator><creator>Symersky, J</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20011101</creationdate><title>A preliminary study of space- and ground-grown insulin crystals by X-ray diffraction and by light scattering tomography</title><author>De Mattei, R.C ; Feigelson, R.S ; Bray, T.L ; DeLucas, L.J ; Symersky, J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-a2f4cd28383be7d3e0344001642cee14932a01de7d52f07a0d22344252a804283</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Aminoacids, peptides. Hormones. Neuropeptides</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Crystal defects</topic><topic>Crystal growth</topic><topic>Deformation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Light scattering</topic><topic>Molecular crystals</topic><topic>Proteins</topic><topic>Tomography</topic><topic>X ray diffraction analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>De Mattei, R.C</creatorcontrib><creatorcontrib>Feigelson, R.S</creatorcontrib><creatorcontrib>Bray, T.L</creatorcontrib><creatorcontrib>DeLucas, L.J</creatorcontrib><creatorcontrib>Symersky, J</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of crystal growth</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>De Mattei, R.C</au><au>Feigelson, R.S</au><au>Bray, T.L</au><au>DeLucas, L.J</au><au>Symersky, J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A preliminary study of space- and ground-grown insulin crystals by X-ray diffraction and by light scattering tomography</atitle><jtitle>Journal of crystal growth</jtitle><date>2001-11-01</date><risdate>2001</risdate><volume>232</volume><issue>1</issue><spage>511</spage><epage>519</epage><pages>511-519</pages><issn>0022-0248</issn><eissn>1873-5002</eissn><coden>JCRGAE</coden><abstract>For the first time, both X-ray diffraction and light scattering tomography were used to study the same set of space- and ground-grown protein crystals. X-ray diffraction of the insulin crystals grown from T6 insulin solutions by temperature reduction showed that space-grown crystals diffract to higher resolution then those grown on the ground. The light scattering tomographs revealed that the space-grown crystals contained very few spherical micro-defects while the ground-grown ones had large numbers of extended micro-defects and dislocations. The tomographs also gave insight into the methods of defect formation. This study marks the beginning of qualification of light scattering tomograph as a tool for determining the suitability of a protein for use in X-ray diffraction studies.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0022-0248(01)01159-9</doi><tpages>9</tpages></addata></record> |
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| ispartof | Journal of crystal growth, 2001-11, Vol.232 (1), p.511-519 |
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| language | eng |
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| source | ScienceDirect Journals (5 years ago - present) |
| subjects | Aminoacids, peptides. Hormones. Neuropeptides Analytical, structural and metabolic biochemistry Biological and medical sciences Crystal defects Crystal growth Deformation Fundamental and applied biological sciences. Psychology General aspects, investigation methods Light scattering Molecular crystals Proteins Tomography X ray diffraction analysis |
| title | A preliminary study of space- and ground-grown insulin crystals by X-ray diffraction and by light scattering tomography |
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