Determination of squalene in olive oil using fractional crystallization for sample preparation
A simple, rapid method for the determination of squalene in virgin olive oil was developed using RP‐HPLC with detection at 208 nm. Fractional crystallization from methanol/acetone (7∶3, vol/vol) was applied to obtain squalene in the liquid fraction of the oil prior to HPLC. Elution of squalene was t...
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| Veröffentlicht in: | Journal of the American Oil Chemists' Society 2002-03, Vol.79 (3), p.257-259 |
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| container_title | Journal of the American Oil Chemists' Society |
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| creator | Nenadis, Nikolaos Tsimidou, Maria |
| description | A simple, rapid method for the determination of squalene in virgin olive oil was developed using RP‐HPLC with detection at 208 nm. Fractional crystallization from methanol/acetone (7∶3, vol/vol) was applied to obtain squalene in the liquid fraction of the oil prior to HPLC. Elution of squalene was then carried out isocratically with acetone/acetonitrile (40∶60 vol/vol) within 11 min. The detection limit was 23 mg/kg, and the limit of quantification 79 mg/kg. The precision of the crystallization procedure (CV%=3.76, n=7) and the mean recovery (92.5 and 81.5% for the 7,000 and 700 mg/kg levels of addition, respectively) were satisfactory. The method is easily applicable to fulfill future needs for nutrition labeling. |
| doi_str_mv | 10.1007/s11746-002-0470-1 |
| format | Article |
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Fractional crystallization from methanol/acetone (7∶3, vol/vol) was applied to obtain squalene in the liquid fraction of the oil prior to HPLC. Elution of squalene was then carried out isocratically with acetone/acetonitrile (40∶60 vol/vol) within 11 min. The detection limit was 23 mg/kg, and the limit of quantification 79 mg/kg. The precision of the crystallization procedure (CV%=3.76, n=7) and the mean recovery (92.5 and 81.5% for the 7,000 and 700 mg/kg levels of addition, respectively) were satisfactory. The method is easily applicable to fulfill future needs for nutrition labeling.</description><identifier>ISSN: 0003-021X</identifier><identifier>EISSN: 1558-9331</identifier><identifier>DOI: 10.1007/s11746-002-0470-1</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer-Verlag</publisher><subject>Acetone ; Acetonitrile ; Biological and medical sciences ; Crystallization ; Fat industries ; Food industries ; Fractional crystallization ; Fractionation ; Fundamental and applied biological sciences. 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Psychology</subject><subject>General aspects</subject><subject>High performance liquid chromatography</subject><subject>Methanol</subject><subject>Methods of analysis, processing and quality control, regulation, standards</subject><subject>nutrition labeling</subject><subject>RP‐HPLC</subject><subject>squalene</subject><subject>virgin olive oil</subject><subject>Volume fraction</subject><issn>0003-021X</issn><issn>1558-9331</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqFkD1PwzAURS0EEqXwA9i8wBbws5M4GavyKVViACQmrIf7jIzcJLVbUPn1pKQSI5N9n-49w2HsFMQFCKEvE4DOy0wImYlciwz22AiKospqpWCfjYQQKhMSXg7ZUUoffayULEbs9YpWFBe-wZVvG946npZrDNQQ930M_pN46wNfJ9-8cxfRbnsYuI2btMIQ_PewdG3kCRddIN5F6jD-no_ZgcOQ6GT3jtnzzfXT9C6bPdzeTyezzOZQ6QzqvHS1dlL02dr-70BospVF9Yb0pnVOFSEoqOsaK1mVIEnPC4Q5FLl1aszOB24X2-Wa0sosfLIUAjbUrpORkJeilrovwlC0sU0pkjNd9AuMGwPCbE2awaTpTZqtSQP95mwHx2Qx9BIa69PfUBWllvmWrYfelw-0-R9sJg_TRyELrX4A2zCFoA</recordid><startdate>200203</startdate><enddate>200203</enddate><creator>Nenadis, Nikolaos</creator><creator>Tsimidou, Maria</creator><general>Springer-Verlag</general><general>Springer</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>200203</creationdate><title>Determination of squalene in olive oil using fractional crystallization for sample preparation</title><author>Nenadis, Nikolaos ; Tsimidou, Maria</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4187-1946f97f20c41cc6f9f107ec8ca3baeb774e8ea131999a828612e7d5a1d154cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Acetone</topic><topic>Acetonitrile</topic><topic>Biological and medical sciences</topic><topic>Crystallization</topic><topic>Fat industries</topic><topic>Food industries</topic><topic>Fractional crystallization</topic><topic>Fractionation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects</topic><topic>High performance liquid chromatography</topic><topic>Methanol</topic><topic>Methods of analysis, processing and quality control, regulation, standards</topic><topic>nutrition labeling</topic><topic>RP‐HPLC</topic><topic>squalene</topic><topic>virgin olive oil</topic><topic>Volume fraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nenadis, Nikolaos</creatorcontrib><creatorcontrib>Tsimidou, Maria</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><jtitle>Journal of the American Oil Chemists' Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nenadis, Nikolaos</au><au>Tsimidou, Maria</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Determination of squalene in olive oil using fractional crystallization for sample preparation</atitle><jtitle>Journal of the American Oil Chemists' Society</jtitle><date>2002-03</date><risdate>2002</risdate><volume>79</volume><issue>3</issue><spage>257</spage><epage>259</epage><pages>257-259</pages><issn>0003-021X</issn><eissn>1558-9331</eissn><abstract>A simple, rapid method for the determination of squalene in virgin olive oil was developed using RP‐HPLC with detection at 208 nm. Fractional crystallization from methanol/acetone (7∶3, vol/vol) was applied to obtain squalene in the liquid fraction of the oil prior to HPLC. Elution of squalene was then carried out isocratically with acetone/acetonitrile (40∶60 vol/vol) within 11 min. The detection limit was 23 mg/kg, and the limit of quantification 79 mg/kg. The precision of the crystallization procedure (CV%=3.76, n=7) and the mean recovery (92.5 and 81.5% for the 7,000 and 700 mg/kg levels of addition, respectively) were satisfactory. The method is easily applicable to fulfill future needs for nutrition labeling.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer-Verlag</pub><doi>10.1007/s11746-002-0470-1</doi><tpages>3</tpages></addata></record> |
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| ispartof | Journal of the American Oil Chemists' Society, 2002-03, Vol.79 (3), p.257-259 |
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| source | Wiley Online Library All Journals; SpringerLink Journals - AutoHoldings |
| subjects | Acetone Acetonitrile Biological and medical sciences Crystallization Fat industries Food industries Fractional crystallization Fractionation Fundamental and applied biological sciences. Psychology General aspects High performance liquid chromatography Methanol Methods of analysis, processing and quality control, regulation, standards nutrition labeling RP‐HPLC squalene virgin olive oil Volume fraction |
| title | Determination of squalene in olive oil using fractional crystallization for sample preparation |
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