Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)

Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)

The intracellular localization of merocyanine 540 (MC540), a photosensitizer commonly used in the photo-inactivation of leukemia cells, was studied using confocal laser scanning microscopy. It was found for the first time that MC540 not only being localized in the plasma membrane but also in the cyt...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:CHIN J LASERS B 2000-06, Vol.B9 (3), p.226-232
Hauptverfasser: Chen, J Y, Cheng, N H, Chen, S C, Fung, M C, Leng, W N, Mak, N K
Format: Artikel
Sprache:eng
Schlagworte:
Carcinogens
Cells
Microscopic examination
Oncology
Scanning
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 232
container_issue 3
container_start_page 226
container_title CHIN J LASERS B
container_volume B9
creator Chen, J Y
Cheng, N H
Chen, S C
Fung, M C
Leng, W N
Mak, N K
description The intracellular localization of merocyanine 540 (MC540), a photosensitizer commonly used in the photo-inactivation of leukemia cells, was studied using confocal laser scanning microscopy. It was found for the first time that MC540 not only being localized in the plasma membrane but also in the cytoplasm and the nuclear membrane of the murine myeloid leukemia M1 and WEHI 3B (JCS) cells. Exposure of MC540 treated leukemia cells to light under conditions that could cause photobleaching did not cause the redistribution of cell-bound MC540. Rapid localization of MC540 in the cytoplasm was observed for 5 minutes after exposure of leukemia cell to MC540, it indicates that MC540 could promptly be internalized by these two leukemia cell lines. In contrast, localization of MC540 was limited only to the plasma membrane of erythrocytes. These results suggest that the binding pattern of MC540 is cell type dependent and may be related to the efficacy of photosensitization in photodynamic therapy.
format Article
fullrecord <record><control><sourceid>proquest</sourceid><recordid>TN_cdi_proquest_miscellaneous_21458541</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>515005</sourcerecordid><originalsourceid>FETCH-LOGICAL-p99t-eed82e6c4a87f0f7ec3e398f1bfb69e924d1bf7bc7ebda29b104ee5bac09df3c3</originalsourceid><addsrcrecordid>eNotjr1qwzAUhT200NDmHe5UksEgy3IsjcX0DxI6JHu4kq-KiCy5lj24b9C3rkM7ncMH5-PcZKuCMZFzyfldtk7JacZ5WVRSqlX2cxyndoYYYOpHvBBgaMFHg9594-gWHi10NEQzY3CBoBIMNodmiS24AN00XGk3k49uWdJ0oc4hGPI-gZ7BxGCvOvCYaIBkMCyeT-icGWIysZ9h0-yPh-1DdmvRJ1r_5312enk-NW_5_uP1vXna571SY07USk47I1DWltmaTEmlkrbQVu8UKS7apdba1KRb5EoXTBBVGg1TrS1NeZ89_mn7IX5NlMZz59L1LQaKUzrzQlSyEkX5CwFbYXA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>21458541</pqid></control><display><type>article</type><title>Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)</title><source>Alma/SFX Local Collection</source><creator>Chen, J Y ; Cheng, N H ; Chen, S C ; Fung, M C ; Leng, W N ; Mak, N K</creator><creatorcontrib>Chen, J Y ; Cheng, N H ; Chen, S C ; Fung, M C ; Leng, W N ; Mak, N K</creatorcontrib><description>The intracellular localization of merocyanine 540 (MC540), a photosensitizer commonly used in the photo-inactivation of leukemia cells, was studied using confocal laser scanning microscopy. It was found for the first time that MC540 not only being localized in the plasma membrane but also in the cytoplasm and the nuclear membrane of the murine myeloid leukemia M1 and WEHI 3B (JCS) cells. Exposure of MC540 treated leukemia cells to light under conditions that could cause photobleaching did not cause the redistribution of cell-bound MC540. Rapid localization of MC540 in the cytoplasm was observed for 5 minutes after exposure of leukemia cell to MC540, it indicates that MC540 could promptly be internalized by these two leukemia cell lines. In contrast, localization of MC540 was limited only to the plasma membrane of erythrocytes. These results suggest that the binding pattern of MC540 is cell type dependent and may be related to the efficacy of photosensitization in photodynamic therapy.</description><identifier>ISSN: 1004-2822</identifier><language>eng</language><subject>Carcinogens ; Cells ; Microscopic examination ; Oncology ; Scanning</subject><ispartof>CHIN J LASERS B, 2000-06, Vol.B9 (3), p.226-232</ispartof><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids></links><search><creatorcontrib>Chen, J Y</creatorcontrib><creatorcontrib>Cheng, N H</creatorcontrib><creatorcontrib>Chen, S C</creatorcontrib><creatorcontrib>Fung, M C</creatorcontrib><creatorcontrib>Leng, W N</creatorcontrib><creatorcontrib>Mak, N K</creatorcontrib><title>Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)</title><title>CHIN J LASERS B</title><description>The intracellular localization of merocyanine 540 (MC540), a photosensitizer commonly used in the photo-inactivation of leukemia cells, was studied using confocal laser scanning microscopy. It was found for the first time that MC540 not only being localized in the plasma membrane but also in the cytoplasm and the nuclear membrane of the murine myeloid leukemia M1 and WEHI 3B (JCS) cells. Exposure of MC540 treated leukemia cells to light under conditions that could cause photobleaching did not cause the redistribution of cell-bound MC540. Rapid localization of MC540 in the cytoplasm was observed for 5 minutes after exposure of leukemia cell to MC540, it indicates that MC540 could promptly be internalized by these two leukemia cell lines. In contrast, localization of MC540 was limited only to the plasma membrane of erythrocytes. These results suggest that the binding pattern of MC540 is cell type dependent and may be related to the efficacy of photosensitization in photodynamic therapy.</description><subject>Carcinogens</subject><subject>Cells</subject><subject>Microscopic examination</subject><subject>Oncology</subject><subject>Scanning</subject><issn>1004-2822</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNotjr1qwzAUhT200NDmHe5UksEgy3IsjcX0DxI6JHu4kq-KiCy5lj24b9C3rkM7ncMH5-PcZKuCMZFzyfldtk7JacZ5WVRSqlX2cxyndoYYYOpHvBBgaMFHg9594-gWHi10NEQzY3CBoBIMNodmiS24AN00XGk3k49uWdJ0oc4hGPI-gZ7BxGCvOvCYaIBkMCyeT-icGWIysZ9h0-yPh-1DdmvRJ1r_5312enk-NW_5_uP1vXna571SY07USk47I1DWltmaTEmlkrbQVu8UKS7apdba1KRb5EoXTBBVGg1TrS1NeZ89_mn7IX5NlMZz59L1LQaKUzrzQlSyEkX5CwFbYXA</recordid><startdate>20000601</startdate><enddate>20000601</enddate><creator>Chen, J Y</creator><creator>Cheng, N H</creator><creator>Chen, S C</creator><creator>Fung, M C</creator><creator>Leng, W N</creator><creator>Mak, N K</creator><scope/></search><sort><creationdate>20000601</creationdate><title>Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)</title><author>Chen, J Y ; Cheng, N H ; Chen, S C ; Fung, M C ; Leng, W N ; Mak, N K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p99t-eed82e6c4a87f0f7ec3e398f1bfb69e924d1bf7bc7ebda29b104ee5bac09df3c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Carcinogens</topic><topic>Cells</topic><topic>Microscopic examination</topic><topic>Oncology</topic><topic>Scanning</topic><toplevel>online_resources</toplevel><creatorcontrib>Chen, J Y</creatorcontrib><creatorcontrib>Cheng, N H</creatorcontrib><creatorcontrib>Chen, S C</creatorcontrib><creatorcontrib>Fung, M C</creatorcontrib><creatorcontrib>Leng, W N</creatorcontrib><creatorcontrib>Mak, N K</creatorcontrib><jtitle>CHIN J LASERS B</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chen, J Y</au><au>Cheng, N H</au><au>Chen, S C</au><au>Fung, M C</au><au>Leng, W N</au><au>Mak, N K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)</atitle><jtitle>CHIN J LASERS B</jtitle><date>2000-06-01</date><risdate>2000</risdate><volume>B9</volume><issue>3</issue><spage>226</spage><epage>232</epage><pages>226-232</pages><issn>1004-2822</issn><abstract>The intracellular localization of merocyanine 540 (MC540), a photosensitizer commonly used in the photo-inactivation of leukemia cells, was studied using confocal laser scanning microscopy. It was found for the first time that MC540 not only being localized in the plasma membrane but also in the cytoplasm and the nuclear membrane of the murine myeloid leukemia M1 and WEHI 3B (JCS) cells. Exposure of MC540 treated leukemia cells to light under conditions that could cause photobleaching did not cause the redistribution of cell-bound MC540. Rapid localization of MC540 in the cytoplasm was observed for 5 minutes after exposure of leukemia cell to MC540, it indicates that MC540 could promptly be internalized by these two leukemia cell lines. In contrast, localization of MC540 was limited only to the plasma membrane of erythrocytes. These results suggest that the binding pattern of MC540 is cell type dependent and may be related to the efficacy of photosensitization in photodynamic therapy.</abstract><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1004-2822
ispartof CHIN J LASERS B, 2000-06, Vol.B9 (3), p.226-232
issn 1004-2822
language eng
recordid cdi_proquest_miscellaneous_21458541
source Alma/SFX Local Collection
subjects Carcinogens
Cells
Microscopic examination
Oncology
Scanning
title Study on uptake and localization of merocyanine 540 (MC540) in murine myeloid leukemia cells by confocal laser scanning microscopy (CLSM)
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-10T14%3A19%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Study%20on%20uptake%20and%20localization%20of%20merocyanine%20540%20(MC540)%20in%20murine%20myeloid%20leukemia%20cells%20by%20confocal%20laser%20scanning%20microscopy%20(CLSM)&rft.jtitle=CHIN%20J%20LASERS%20B&rft.au=Chen,%20J%20Y&rft.date=2000-06-01&rft.volume=B9&rft.issue=3&rft.spage=226&rft.epage=232&rft.pages=226-232&rft.issn=1004-2822&rft_id=info:doi/&rft_dat=%3Cproquest%3E515005%3C/proquest%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=21458541&rft_id=info:pmid/&rfr_iscdi=true