Optical fiber bioluminescent whole-cell microbial biosensors to genotoxicants
Conservation of water resources calls for ever stricter regulatory measures and better monitoring systems. Whole-cell bacterial sensors have been genetically engineered to react to target toxicants by the induction of a selected promoter and the subsequent production of bioluminescent light through...
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| Veröffentlicht in: | Water science and technology 2000-01, Vol.42 (1-2), p.305-311 |
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| container_title | Water science and technology |
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| creator | POLYAK, B BASSIS, E NOVODVORETS, A BELKIN, S MARKS, R. S |
| description | Conservation of water resources calls for ever stricter regulatory measures and better monitoring systems. Whole-cell bacterial sensors have been genetically engineered to react to target toxicants by the induction of a selected promoter and the subsequent production of bioluminescent light through a recombinant lux reporter. In order to create a one-step assay, we have designed a new, self-contained, disposable optical fiber sensor module and a customized photodetector system that integrates these microorganisms. A photon-counting photomultiplier tube-based instrument was constructed. Optical fiber tip cores were covered with adlayer films consisting of calcium alginate containing bioluminescent bacterial sensors of genotoxicants. Multiplying these steps thickened the adlayer in increments, increasing the number of bacterial reporters attached to the optical fiber transducer. These whole cell optrodes are responsive to external traces of DNA damaging agents such as mitomycin C. Light production was shown to be dose-dependent and proportional to the number of bacterial layers. |
| doi_str_mv | 10.2166/wst.2000.0330 |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 0273-1223 |
| ispartof | Water science and technology, 2000-01, Vol.42 (1-2), p.305-311 |
| issn | 0273-1223 1996-9732 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_21449424 |
| source | Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Alginic acid Applied sciences Bacteria Bioassay Bioluminescence Biosensors Calcium Calcium alginate Calcium compounds Customization Damage Deoxyribonucleic acid DNA DNA damage Exact sciences and technology Genetic engineering Genotoxicity Luminescence Microorganisms Mitomycin C Monitoring systems Natural water pollution Optical fibers Photodetectors Photomultiplier tubes Photomultipliers Pollution Recombinants Seaweed meal Sensors Toxic materials Toxicants Toxicity Transducers Water conservation Water resources Water treatment and pollution |
| title | Optical fiber bioluminescent whole-cell microbial biosensors to genotoxicants |
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