A Novel Activity of Immobilized Penicillin G Acylase: Removal of Benzyloxycarbonyl Amino Protecting Group
A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantita...
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| Veröffentlicht in: | Biocatalysis and biotransformation 2000, Vol.18 (3), p.253-258 |
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| container_end_page | 258 |
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| container_issue | 3 |
| container_start_page | 253 |
| container_title | Biocatalysis and biotransformation |
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| creator | Alvaro, Gregorio Feliu, Josep A. Caminal, Gloria López-santín, Josep Clapés, Pere |
| description | A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantitative deprotection yields were obtained for simple amino acids, while the efficiency with oligopeptides varied depending on the sequence. |
| doi_str_mv | 10.3109/10242420009015248 |
| format | Article |
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This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantitative deprotection yields were obtained for simple amino acids, while the efficiency with oligopeptides varied depending on the sequence.</description><identifier>ISSN: 1024-2422</identifier><identifier>EISSN: 1029-2446</identifier><identifier>DOI: 10.3109/10242420009015248</identifier><language>eng</language><publisher>Abingdon: Informa UK Ltd</publisher><subject>Amino acids ; Antibiotics ; benzyloxycarbonyl amino protecting group ; Bioconversions. Hemisynthesis ; Biological and medical sciences ; Biotechnology ; Enzyme kinetics ; Enzymes and enzyme reactions ; Escherichia coli ; Fundamental and applied biological sciences. Psychology ; Hydrolysis ; Methods. Procedures. Technologies ; oligopeptides ; Organic solvents ; penicillin G acylase ; Peptides and polypeptides ; Polypeptides ; Synthesis (chemical) ; Urethane protecting groups</subject><ispartof>Biocatalysis and biotransformation, 2000, Vol.18 (3), p.253-258</ispartof><rights>2000 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 2000</rights><rights>2001 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-187b8e36d2008db0623115203319eebf934ff867bb21074b74612400d6ab4d483</citedby><cites>FETCH-LOGICAL-c438t-187b8e36d2008db0623115203319eebf934ff867bb21074b74612400d6ab4d483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10242420009015248$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10242420009015248$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=907143$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Alvaro, Gregorio</creatorcontrib><creatorcontrib>Feliu, Josep A.</creatorcontrib><creatorcontrib>Caminal, Gloria</creatorcontrib><creatorcontrib>López-santín, Josep</creatorcontrib><creatorcontrib>Clapés, Pere</creatorcontrib><title>A Novel Activity of Immobilized Penicillin G Acylase: Removal of Benzyloxycarbonyl Amino Protecting Group</title><title>Biocatalysis and biotransformation</title><description>A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantitative deprotection yields were obtained for simple amino acids, while the efficiency with oligopeptides varied depending on the sequence.</description><subject>Amino acids</subject><subject>Antibiotics</subject><subject>benzyloxycarbonyl amino protecting group</subject><subject>Bioconversions. Hemisynthesis</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Enzyme kinetics</subject><subject>Enzymes and enzyme reactions</subject><subject>Escherichia coli</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrolysis</subject><subject>Methods. Procedures. Technologies</subject><subject>oligopeptides</subject><subject>Organic solvents</subject><subject>penicillin G acylase</subject><subject>Peptides and polypeptides</subject><subject>Polypeptides</subject><subject>Synthesis (chemical)</subject><subject>Urethane protecting groups</subject><issn>1024-2422</issn><issn>1029-2446</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><recordid>eNqFkV1LwzAUhoso-PkDvAsI3k3ztaZVb6boHIgO0euStKcaSZOZdNPu15u5KYig5CKH8LzvOedNkuwTfMQIzo8JpjwejHGOSZ_ybC3Zim95j3Kern_WPNaUbibbIbxgTBjNxVaiB-jWzcCgQdnqmW475Go0ahqntNFzqNAYrC61MdqiYYQ6IwOcoHto3EyaBXwOdt4Z996V0itnu2jVaOvQ2LsWoql9QkPvppPdZKOWJsDe6t5JHq8uHy6uezd3w9HF4KZXcpa1PZIJlQFLq7hLVimcUkbiQpgxkgOoOme8rrNUKEUJFlwJnhLKMa5SqXjFM7aTHC59J969TiG0RaNDCcZIC24aCko4EyzL_wWJEKwfvSNIlmDpXQge6mLidSN9VxBcLNIvfqUfNQcrcxlKaWovbanDtzDHIo4RqbMlpW3tfCPfnDdV0cqYp_-SsL-anP6QP4M07XP8Byhe3NTbGPMfI34Asfyn3Q</recordid><startdate>2000</startdate><enddate>2000</enddate><creator>Alvaro, Gregorio</creator><creator>Feliu, Josep A.</creator><creator>Caminal, Gloria</creator><creator>López-santín, Josep</creator><creator>Clapés, Pere</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T7</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>2000</creationdate><title>A Novel Activity of Immobilized Penicillin G Acylase: Removal of Benzyloxycarbonyl Amino Protecting Group</title><author>Alvaro, Gregorio ; Feliu, Josep A. ; Caminal, Gloria ; López-santín, Josep ; Clapés, Pere</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-187b8e36d2008db0623115203319eebf934ff867bb21074b74612400d6ab4d483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino acids</topic><topic>Antibiotics</topic><topic>benzyloxycarbonyl amino protecting group</topic><topic>Bioconversions. Hemisynthesis</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Enzyme kinetics</topic><topic>Enzymes and enzyme reactions</topic><topic>Escherichia coli</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrolysis</topic><topic>Methods. Procedures. Technologies</topic><topic>oligopeptides</topic><topic>Organic solvents</topic><topic>penicillin G acylase</topic><topic>Peptides and polypeptides</topic><topic>Polypeptides</topic><topic>Synthesis (chemical)</topic><topic>Urethane protecting groups</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Alvaro, Gregorio</creatorcontrib><creatorcontrib>Feliu, Josep A.</creatorcontrib><creatorcontrib>Caminal, Gloria</creatorcontrib><creatorcontrib>López-santín, Josep</creatorcontrib><creatorcontrib>Clapés, Pere</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>Biocatalysis and biotransformation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Alvaro, Gregorio</au><au>Feliu, Josep A.</au><au>Caminal, Gloria</au><au>López-santín, Josep</au><au>Clapés, Pere</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A Novel Activity of Immobilized Penicillin G Acylase: Removal of Benzyloxycarbonyl Amino Protecting Group</atitle><jtitle>Biocatalysis and biotransformation</jtitle><date>2000</date><risdate>2000</risdate><volume>18</volume><issue>3</issue><spage>253</spage><epage>258</epage><pages>253-258</pages><issn>1024-2422</issn><eissn>1029-2446</eissn><abstract>A novel activity of penicillin G acylase from E. coli is presented. This enzyme, immobilized onto agarose gels by multipoint covalent attachment, was used for removing the benzyloxycarbonyl (Z) amino protecting group from selected amino acids and oligopeptides in different reaction systems. Quantitative deprotection yields were obtained for simple amino acids, while the efficiency with oligopeptides varied depending on the sequence.</abstract><cop>Abingdon</cop><pub>Informa UK Ltd</pub><doi>10.3109/10242420009015248</doi><tpages>6</tpages></addata></record> |
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| ispartof | Biocatalysis and biotransformation, 2000, Vol.18 (3), p.253-258 |
| issn | 1024-2422 1029-2446 |
| language | eng |
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| source | Taylor & Francis Journals Complete |
| subjects | Amino acids Antibiotics benzyloxycarbonyl amino protecting group Bioconversions. Hemisynthesis Biological and medical sciences Biotechnology Enzyme kinetics Enzymes and enzyme reactions Escherichia coli Fundamental and applied biological sciences. Psychology Hydrolysis Methods. Procedures. Technologies oligopeptides Organic solvents penicillin G acylase Peptides and polypeptides Polypeptides Synthesis (chemical) Urethane protecting groups |
| title | A Novel Activity of Immobilized Penicillin G Acylase: Removal of Benzyloxycarbonyl Amino Protecting Group |
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